BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	543,804	T→C	H180R (CAT→CGT)	allC ←	allantoate deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	543,804	0	T	C	99.8%	1959.8 / NA	562	H180R (CAT→CGT)	allC	allantoate deiminase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (345/216); minor base A (1/0); total (346/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 571 total aligned reads displayed.

GGTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGAA  >  NZ_CP025268/543667‑543940

ggTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGc                                                                                                                                        >  1:7967465/1‑140 (MQ=255)

ggTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGc                                                                                                                                        <  2:490673/140‑1 (MQ=255)

 gTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATTTGCAGTTCAACAAAGGCTTTAATATCCTGAcgcg                                                                                                                                       >  2:8997832/1‑140 (MQ=255)

  tttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGTCTTTAATATCCTGACGCgg                                                                                                                                      <  2:3162079/140‑1 (MQ=255)

  tttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCgg                                                                                                                                      <  1:2935491/140‑1 (MQ=255)

  tttGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGGTCAACAAAGGCTTTAATATCCTGACGCgg                                                                                                                                      >  2:933933/1‑140 (MQ=255)

    tGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAg                                                                                                                                    <  1:5311710/140‑1 (MQ=255)

      aTTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGtt                                                                                                                                  >  2:9721506/1‑140 (MQ=255)

            cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTggg                                                                                                                            >  1:5998800/1‑140 (MQ=255)

            cGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTggg                                                                                                                            >  2:1740741/1‑140 (MQ=255)

               tCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCg                                                                                                                         <  1:3998938/140‑1 (MQ=255)

                    gTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGAGTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCGGACGCGGAGTTAGTGGGGCGTTCgg                                                                                                                    <  2:3874283/140‑1 (MQ=255)

                            ataACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTaa                                                                                                            <  2:2454285/140‑1 (MQ=255)

                             taACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGTTTGCCCATTACTTTCCAGCACACGGCCCGGGTCTAGATGCAGGTCAACATAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAGGAGGaag                                                                                                           >  2:4698103/1‑139 (MQ=255)

                             taACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTaaa                                                                                                           >  2:4571401/1‑140 (MQ=255)

                                cgacgCTGCCCGACAATTTCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCTGTTCAACAAAGGCTTTATTATCCTGACGCGGTGGTAGTGGGGCGTTCGGAAGAGTAATTcg                                                                                                        >  2:8016762/1‑139 (MQ=255)

                                     cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAg                                                                                                   >  2:6102094/1‑140 (MQ=255)

                                     cTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAg                                                                                                   >  2:2788177/1‑140 (MQ=255)

                                       gCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGcc                                                                                                 >  2:5045545/1‑140 (MQ=255)

                                        cccGACAATTGCATTGACCACGCCAATTGATTGCCCAATACTTTACAGCACACAGCCCTGATCAATATGCAGGTAAGCAAAGGCTTAATTATCATGACGCGGAGTGAGTGGGGCGTGCGGCCGAGTCAAGCAGCACGccg                                                                                                >  2:11205748/1‑139 (MQ=255)

                                             cAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCGGTTCAAGATGCAGTTCAACAAAGGCTTTCATATCCTGACGCGGAGTTTGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTGcatc                                                                                           >  2:811684/1‑140 (MQ=255)

                                                  gCATTCACCACGCCAATTTATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTGTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTCAATCCGCAAGCCTTcatcgcatc                                                                                      >  2:2209082/1‑140 (MQ=255)

                                                  gCATTCACCACGCCAATTGAGTGCCCATGACTGTCCAGCACACAGCCCTGGGCCATCTGCAGTTCAACAAAGGCTTGAATCGCCTGACGCGGCGTTAGAGGGGCGTGCGGAAGAGTCACTCCGCAAGCCTGcctcgcagc                                                                                      >  2:7706945/1‑138 (MQ=255)

                                                        accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaa                                                                                >  1:10909478/1‑140 (MQ=255)

                                                        accacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaa                                                                                >  1:8373905/1‑140 (MQ=255)

                                                          cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAAc                                                                              >  1:6326248/1‑140 (MQ=255)

                                                            cGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTa                                                                            >  2:5828223/1‑140 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                           >  2:425953/1‑140 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                           >  1:11240474/1‑140 (MQ=255)

                                                             gCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGAAGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAt                                                                           >  2:5369387/1‑140 (MQ=255)

                                                                  ttgattgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCt                                                                      >  1:464928/1‑140 (MQ=255)

                                                                      ttgCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                                  >  2:2313684/1‑140 (MQ=255)

                                                                      ttgCCCATTACTTTCCAGCACACAGCCCTGTTCAAGATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTgg                                                                  <  2:10380320/140‑1 (MQ=255)

                                                                         cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                               <  1:10025498/140‑1 (MQ=255)

                                                                         cccATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt                                                               <  1:10707340/140‑1 (MQ=255)

                                                                               aCTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAATACTATTTCCTTTGGCTTAACAGa                                                         >  2:10511488/1‑140 (MQ=255)

                                                                               aCTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGa                                                         >  2:10147261/1‑140 (MQ=255)

                                                                                   tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATAtt                                                     >  2:6899908/1‑140 (MQ=255)

                                                                                     cAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTcc                                                   >  1:7164156/1‑140 (MQ=255)

                                                                                       gCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc                                                 >  1:4382412/1‑140 (MQ=255)

                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                                <  1:1740741/140‑1 (MQ=255)

                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                                <  1:2263923/140‑1 (MQ=255)

                                                                                        cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCa                                                <  2:11386323/140‑1 (MQ=255)

                                                                                         acacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAc                                               <  2:9585004/140‑1 (MQ=255)

                                                                                          cacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcg                                              >  2:7120253/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  1:6654814/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  2:10194484/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  1:7566729/1‑140 (MQ=255)

                                                                                             aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTAATTCCTTTGGCATCACAGATATTCCGCAcgtcg                                           >  1:9638757/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:9221831/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  1:11081388/1‑140 (MQ=255)

                                                                                               cccTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGGTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACgtcgtc                                         >  2:2861480/1‑140 (MQ=255)

                                                                                                  tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAgg                                      >  1:7021393/1‑140 (MQ=255)

                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc                                                 >  1:2464989/1‑127 (MQ=255)

                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                    >  2:2077179/1‑140 (MQ=255)

                                                                                                    ttCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGAt                                    >  1:8552772/1‑140 (MQ=255)

                                                                                                        atatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc                                <  1:7367055/140‑1 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGNGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:6171365/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:2006358/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  2:626/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  1:8226830/1‑140 (MQ=255)

                                                                                                         tatGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGcc                               >  2:8679936/1‑140 (MQ=255)

                                                                                                            gCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGc                            >  2:1879310/1‑140 (MQ=255)

                                                                                                            gCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGc                            <  1:8080879/140‑1 (MQ=255)

                                                                                                            gCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGc                            >  2:356396/1‑140 (MQ=255)

                                                                                                              aGTTCTACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGTCGTTCGGTAGAGTAAATCCGAAAGCCTTCATCGGATCGACAAATCTATTTCCGTTGGCATCACAGATCTTGAGATCGTCGTCAGGATTCGCCAGccg                          >  2:6777123/1‑139 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:129250/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:3180890/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  2:2601450/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:3349850/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:6543105/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:5470317/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  1:5769915/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGccc                          >  2:1222260/1‑140 (MQ=255)

                                                                                                              aGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTAGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTACGAACGTCGTCAGGATTCTCCAGccc                          >  2:6460871/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATca                                                             <  2:6609528/103‑1 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                        >  1:3861740/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                        >  1:9313339/1‑140 (MQ=255)

                                                                                                                ttCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa                        >  2:1481056/1‑140 (MQ=255)

                                                                                                                 tCAACAAAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaa                       <  2:8480438/140‑1 (MQ=255)

                                                                                                                   aacaaAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaaaaa                     >  2:7330267/1‑140 (MQ=255)

                                                                                                                     caaAGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAata                   >  1:8765782/1‑140 (MQ=255)

                                                                                                                       aaGGCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtt                 >  2:10575146/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:10175967/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:11561422/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:9904347/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:4828753/1‑140 (MQ=255)

                                                                                                                          gCTTTAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCATCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAttttt              >  1:11390308/1‑140 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  2:11464280/140‑1 (MQ=255)

                                                                                                                            tttAATATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTAc            <  2:3740856/140‑1 (MQ=255)

                                                                                                                               aaTATCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCGTCATCGCATCGACAAATCTATTTCCTTTGGCCTCTCAGATATGCCGCACGTCGTGAGGTTTCGCCAGCCCAATAATATTTTTACTGc         >  2:10902367/1‑140 (MQ=255)

                                                                                                                                 tatCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGccc       <  1:3726511/140‑1 (MQ=255)

                                                                                                                                 tatCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGccc       <  2:3440622/140‑1 (MQ=255)

                                                                                                                                   tCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa     <  2:5601860/140‑1 (MQ=255)

                                                                                                                                   tCCTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa     <  1:2678325/140‑1 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag    >  2:4065452/1‑140 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCag    <  1:9075278/140‑1 (MQ=255)

                                                                                                                                    ccTGACGCGGAGTTAGTGGGGCGTTAGGAAGAGTAAATCCGCAAGCCTACATCGCATCGACAAAACTAATTCCTTTGTCATAACAGATATTCCGCACGACGTCAGGATTCGCAAGCCCAAAAATATATTTACTGCCCCag    >  2:1628303/1‑140 (MQ=255)

                                                                                                                                     cTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCaga   <  1:1644184/140‑1 (MQ=255)

                                                                                                                                     cTGACGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCaga   <  1:10495058/140‑1 (MQ=255)

GGTTTGATTCGCCGTTCAGCGTTACCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCAGAA  >  NZ_CP025268/543667‑543940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`