BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	832,209	A→G	V336A (GTG→GCG)	ybhR ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	832,209	0	A	G	99.9%	2259.4 / NA	650	V336A (GTG→GCG)	ybhR	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (366/283); minor base T (0/1); total (366/284)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 665 total aligned reads displayed.

CCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832072‑832345

cccggcGGTAGTGTCTTTGCTGGCGAAAGATACGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCCGACTcgca                                                                                                                                        >  1:7970014/1‑140 (MQ=255)

cccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTcgca                                                                                                                                        <  1:10084917/140‑1 (MQ=255)

cccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTcgca                                                                                                                                        >  1:6729187/1‑140 (MQ=255)

 ccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAt                                                                                                                                       >  2:4776982/1‑140 (MQ=255)

 ccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCAt                                                                                                                                       <  2:2908995/140‑1 (MQ=255)

    gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    <  1:11171592/140‑1 (MQ=255)

       gTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCa                                                                                                                                 <  1:1815630/140‑1 (MQ=255)

            gtCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                                                                            <  2:1508979/140‑1 (MQ=255)

            gtCTGTGCTGGCGAAAGATAAGAAGTGACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                                                                            <  1:10236619/140‑1 (MQ=255)

             tCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaa                                                                                                                           >  2:6247365/1‑140 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  2:5870244/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:1255660/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:9373252/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCGTACGTCTAACCATCGCGTACGCCGCTGACACGGTCGTGGCCGTTCTCACCAGTAGCGGCCACAAACTATTCCACCCAATATCCAGCCTCGCAGCCTTCaaataaat                                                                                                                          <  2:854550/140‑1 (MQ=255)

                ttGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCt                                                                                                                        <  2:1859795/140‑1 (MQ=255)

                 tGCTGGCGCAAGATAAGACGTTACCTCACCGACCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTCGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTg                                                                                                                       <  2:947014/140‑1 (MQ=255)

                  gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                                                                      >  1:9612499/1‑140 (MQ=255)

                     ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTg                                                                                                                   >  2:10817715/1‑140 (MQ=255)

                     ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTg                                                                                                                   >  1:4919856/1‑140 (MQ=255)

                       cGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGt                                                                                                                 <  1:5706788/140‑1 (MQ=255)

                         aaaGATAAGACGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  1:11108519/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAACCATCGCGTACGCCGCTGACCCGGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTAGTCCACACAATATCCCGACTCGCATCCTTCAACTAAATCTGCTTGGTaa                                                                                                               <  2:6603887/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAACTAAATCTGCTTGGTaa                                                                                                               <  2:1822256/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  1:3332880/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  2:220977/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  1:8513727/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  2:3683655/140‑1 (MQ=255)

                                 gaagTTACCTCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                       <  2:7038115/140‑1 (MQ=255)

                                 gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                       <  1:2689492/140‑1 (MQ=255)

                                      tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  2:5250154/140‑1 (MQ=255)

                                       aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  1:5593272/1‑140 (MQ=255)

                                       aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  1:7419645/1‑140 (MQ=255)

                                       aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCCTTATCACCAGTAGCGGCCACAAACTATTCCACACAAAATCCAGACTCGCATCCTTCAAATAAATCAGCTAGGTAATGTCCGTAAAGttg                                                                                                 >  1:7199712/1‑138 (MQ=255)

                                           cACCTTACGTCTCCACAGCCCCTCCGCCCCCGACCCTGTCGTCGCCGCTATCACCCCTAGCGGCCCCACACTATTCCACACACGATCCAGACCCGCATCCTTCAACAACAACGGCGAGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  2:510221/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:4452913/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  2:8196060/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:14100/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTGCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  2:10001502/140‑1 (MQ=255)

                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  1:3464460/140‑1 (MQ=255)

                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  2:679988/140‑1 (MQ=255)

                                              cTTACGTCTCAACCTCGCGAACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTCTTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAg                                                                                          <  2:6165043/140‑1 (MQ=255)

                                              cTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAg                                                                                          >  1:2024886/1‑140 (MQ=255)

                                                 aCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                                                                       <  1:10604036/140‑1 (MQ=255)

                                                  cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTCAAGTGGCGAATAGGGtt                                                                                      >  2:3082244/1‑140 (MQ=255)

                                                     cTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                                                                   >  1:8915562/1‑140 (MQ=255)

                                                       aaaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                                 >  2:737143/1‑140 (MQ=255)

                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa                                                                                >  2:3870949/1‑140 (MQ=255)

                                                        aaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCa                                                                                >  1:3549417/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  1:9240711/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  1:6918924/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  2:11011343/1‑140 (MQ=255)

                                                                 tACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                                                                       >  1:702067/1‑140 (MQ=255)

                                                                 tACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGtt                                                                       >  1:10383057/1‑140 (MQ=255)

                                                                       gcTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCCCAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCGTGGTAAGGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTGTTGCAg                                                                 >  2:9261377/1‑140 (MQ=255)

                                                                           aCCCTTACGTGGCCGATATCACCAGAAGAGGCCACAAACTATTCCCCACAATATCCAGACTCGCATCCTTCAAAAAAATCTGCGTGGTAATGTCCGTAAAGTGGCGACTAGGGGTAATCAACGTCAGGTGTTGCAGCCAt                                                             >  2:11626109/1‑140 (MQ=255)

                                                                            cccTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAAACTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATa                                                            <  1:2873202/140‑1 (MQ=255)

                                                                              cTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATAcc                                                          >  1:4219158/1‑140 (MQ=255)

                                                                              cTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGACATAcc                                                          >  2:8971434/1‑140 (MQ=255)

                                                                               tGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCg                                                         >  1:6001712/1‑140 (MQ=255)

                                                                                     ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAATTAAATCTGCTTGGTACTGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCAGGt                                                   >  2:8410948/1‑140 (MQ=255)

                                                                                     ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt                                                   >  1:11592252/1‑140 (MQ=255)

                                                                                      gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGtt                                                  >  2:105182/1‑140 (MQ=255)

                                                                                      gCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGtt                                                  >  2:760714/1‑140 (MQ=255)

                                                                                         gTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTc                                               >  2:5707727/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:4046469/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:6408382/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:9318673/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:10714136/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAAGGTCCGTATAGTGGCGAATAGGGGTAATCAACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:3308980/1‑140 (MQ=255)

                                                                                            aTCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCAc                                            <  2:1246491/140‑1 (MQ=255)

                                                                                                    tAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGaaa                                    >  1:236838/1‑140 (MQ=255)

                                                                                                     aGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                   <  1:11426436/140‑1 (MQ=255)

                                                                                                      gCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACg                                  >  2:11285009/1‑140 (MQ=255)

                                                                                                       cGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAATCGt                                 >  1:8338418/1‑140 (MQ=255)

                                                                                                       cGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGt                                 <  1:381393/140‑1 (MQ=255)

                                                                                                         gacacaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTTCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               <  2:7896542/138‑1 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTATAGTGGCGAATAGGGTTAATCCGCGTCAGGTGTTGCAGCCATAGCGGCATGTTTTCCACCGGTGAAGCggtc                               >  1:6381283/1‑137 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  2:4016842/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  1:2533158/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  1:8169972/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               <  1:2058625/140‑1 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACGCGCATCCTTCAAATAGATCTGCTGGGTACTGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCCTGTGTTCCACCGGAGAACCGtcc                               >  1:4746075/1‑138 (MQ=255)

                                                                                                           cacaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAAcc                             >  2:2678161/1‑140 (MQ=255)

                                                                                                            acaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCg                            <  1:191692/140‑1 (MQ=255)

                                                                                                               aaCTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAACGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGaa                         <  1:1614805/140‑1 (MQ=255)

                                                                                                                    ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  1:5341806/1‑140 (MQ=255)

                                                                                                                    ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  2:8820614/1‑140 (MQ=255)

                                                                                                                    ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  2:4564459/1‑140 (MQ=255)

                                                                                                                     tCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgaga                   >  1:5169476/1‑140 (MQ=255)

                                                                                                                        acacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAACGGAGAATg                >  2:2068023/1‑140 (MQ=255)

                                                                                                                         cacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGACACGTAACCGGAAAGGAGAATgg               >  2:1765773/1‑140 (MQ=255)

                                                                                                                         cacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATgg               >  1:9106870/1‑140 (MQ=255)

                                                                                                                          acaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTACCCGGACAGGAGAATGGc              >  2:7186396/1‑140 (MQ=255)

                                                                                                                          acaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGc              <  1:9764908/140‑1 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAANGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            >  1:10775088/1‑140 (MQ=255)

                                                                                                                                tCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcat        >  2:4310731/1‑140 (MQ=255)

                                                                                                                                 ccAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatc       >  1:3131880/1‑140 (MQ=255)

                                                                                                                                    gACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             <  2:4138421/99‑1 (MQ=255)

                                                                                                                                    gACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATa    <  1:8818743/140‑1 (MQ=255)

                                                                                                                                      cTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATaaa  >  2:2522350/1‑140 (MQ=255)

CCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832072‑832345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`