BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,075,358	A→G	58 (TAA→TAG)	ymdF →	general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,075,358	0	A	G	99.6%	1922.4 / NA	553	58 (TAA→TAG)	ymdF	general stress protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (230/320); minor base T (2/0); total (232/321)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 553 total aligned reads displayed.

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAA‑T‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075221‑1075491

   gaCCTCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                     <  1:5378038/140‑1 (MQ=255)

   gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                     <  1:4919115/140‑1 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                  >  1:2391110/1‑140 (MQ=255)

      cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                  >  1:11628319/1‑140 (MQ=255)

               gCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGAc                                                                                                                         <  1:2734434/140‑1 (MQ=255)

                cATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACg                                                                                                                        >  1:3859255/1‑140 (MQ=255)

                 aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGa                                                                                                                       <  2:2547554/140‑1 (MQ=255)

                 aTCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGa                                                                                                                       <  1:9538622/140‑1 (MQ=255)

                          aGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAtttt                                                                                                             <  1:6461228/140‑1 (MQ=255)

                          aGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAtttt                                                                                                             >  2:9612448/1‑140 (MQ=255)

                          aGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAtttt                                                                                                             <  1:8891734/140‑1 (MQ=255)

                          aGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAtttt                                                                                                             <  1:11178589/140‑1 (MQ=255)

                           ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAttttt                                                                                                            >  2:1542603/1‑140 (MQ=255)

                           ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAttttt                                                                                                            >  2:7500235/1‑140 (MQ=255)

                                    ggTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGc                                                                                                   <  2:7781436/140‑1 (MQ=255)

                                     gTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCt                                                                                                  >  2:1275915/1‑140 (MQ=255)

                                      tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTc                                                                                                 >  1:1845698/1‑140 (MQ=255)

                                      tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTc                                                                                                 >  2:5036221/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCaaaa                                                                                          >  2:11323069/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCaaaa                                                                                          >  2:4512780/1‑140 (MQ=255)

                                             cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCaaaa                                                                                          <  1:11109603/140‑1 (MQ=255)

                                                  cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCa                                                                                     >  2:3194543/1‑140 (MQ=255)

                                                     gggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCa                                                                                  >  2:10132224/1‑140 (MQ=255)

                                                      ggTAATTTCAAGATTGACCCGCAGCGCGCATCTGAAGCTGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAc                                                                                 <  1:8374041/140‑1 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  2:5287196/1‑140 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                <  2:3586147/140‑1 (MQ=255)

                                                       gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:7413446/1‑140 (MQ=255)

                                                         aaTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACGACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgc                                                                              <  1:11019309/140‑1 (MQ=255)

                                                            ttCAAGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                           >  1:4766018/1‑140 (MQ=255)

                                                            ttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                           <  1:10917542/140‑1 (MQ=255)

                                                               aaGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTa                                                                        >  1:5816349/1‑140 (MQ=255)

                                                                aGAATGACCCGCCGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAc                                                                       >  1:11454609/1‑140 (MQ=255)

                                                                 gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  1:4512780/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:9640177/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:10031542/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:5722752/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:3425293/140‑1 (MQ=255)

                                                                   aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:106782/140‑1 (MQ=255)

                                                                     gACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggt                                                                  <  2:5806205/140‑1 (MQ=255)

                                                                      aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  2:10032204/1‑140 (MQ=255)

                                                                          gCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTg                                                             <  2:4094758/140‑1 (MQ=255)

                                                                          gCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTg                                                             <  2:9509447/140‑1 (MQ=255)

                                                                           cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                            >  2:10330884/1‑140 (MQ=255)

                                                                           cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                            <  1:7155731/140‑1 (MQ=255)

                                                                             gcgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                          <  1:7302271/140‑1 (MQ=255)

                                                                               gcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                        >  1:8647859/1‑140 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATTCtg                                                       >  2:7092775/1‑140 (MQ=255)

                                                                                cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       >  2:6109441/1‑140 (MQ=255)

                                                                                 gcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtga                                                      <  2:4614661/140‑1 (MQ=255)

                                                                                   aTCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAACAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatg                                                    <  2:2084597/140‑1 (MQ=255)

                                                                                    tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatga                                                   <  1:6816618/140‑1 (MQ=255)

                                                                                    tCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatga                                                   <  2:11119788/140‑1 (MQ=255)

                                                                                     cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  2:2253642/140‑1 (MQ=255)

                                                                                         aGCAGGTCAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  2:10921399/140‑1 (MQ=255)

                                                                                         aGCAGGTACAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACGAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  1:8526473/140‑1 (MQ=255)

                                                                                         aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACTGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAATTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  2:6279661/140‑1 (MQ=255)

                                                                                         aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  2:6811608/140‑1 (MQ=255)

                                                                                         aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  1:9460931/140‑1 (MQ=255)

                                                                                         aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  1:5042434/140‑1 (MQ=255)

                                                                                         aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  2:2934206/140‑1 (MQ=255)

                                                                                          gCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATg                                             <  1:151913/140‑1 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAATAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:7638517/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:9097212/1‑140 (MQ=255)

                                                                                             ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:5480065/1‑140 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCTACAACTAGCAGGGCTAATCAATGACGAA‑TGAATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         >  1:7827167/1‑140 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  2:10575597/140‑1 (MQ=255)

                                                                                              gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  2:1745346/140‑1 (MQ=255)

                                                                                                      ggtggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTg                                 <  1:3132528/140‑1 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTggg                               >  1:6234483/1‑138 (MQ=255)

                                                                                                        tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  2:2758251/1‑140 (MQ=255)

                                                                                                         ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  1:10252931/1‑140 (MQ=255)

                                                                                                         ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              <  1:2152515/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTTCCTGATgcg                            <  1:138115/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:7400578/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:256712/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:9163915/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:7571426/140‑1 (MQ=255)

                                                                                                           tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGATAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:6394380/140‑1 (MQ=255)

                                                                                                           gaaGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:1399214/139‑1 (MQ=255)

                                                                                                              gagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGa                         <  2:8292190/140‑1 (MQ=255)

                                                                                                               agCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGAt                        >  2:10700104/1‑140 (MQ=255)

                                                                                                                 cAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACt                                                        <  2:6454640/106‑1 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   <  2:3859255/140‑1 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   >  1:5898829/1‑140 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   >  2:1162331/1‑140 (MQ=255)

                                                                                                                    tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGACAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   <  1:366114/140‑1 (MQ=255)

                                                                                                                     cACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                  >  2:8796639/1‑140 (MQ=255)

                                                                                                                       cGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCa                <  2:9871190/140‑1 (MQ=255)

                                                                                                                         gCAAAATCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:1725627/140‑1 (MQ=255)

                                                                                                                         gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  2:3302662/140‑1 (MQ=255)

                                                                                                                          cAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGc             <  1:8136469/140‑1 (MQ=255)

                                                                                                                              aGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         <  2:5531325/140‑1 (MQ=255)

                                                                                                                              aGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         >  1:4154281/1‑140 (MQ=255)

                                                                                                                                cGACAACTCGCCGGGCTAATCACTGACGAA‑TGCATTTTTGTCTGTAGCACCTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAACTGGAAGCTGGCCAGATGCGCGATGCTTATCAGGCCTAccc       <  2:8916650/140‑2 (MQ=255)

                                                                                                                                 gACAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCag      <  2:7893971/140‑1 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACGAAGG‑CATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  1:10109485/140‑1 (MQ=255)

                                                                                                                                   cAACTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  2:1835657/140‑1 (MQ=255)

                                                                                                                                    aaCTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaag   >  1:2882077/1‑140 (MQ=255)

                                                                                                                                     aCTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:334585/140‑1 (MQ=255)

                                                                                                                                     aCTAGCCGGGCTAATCAATGACGAA‑TGCATTTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  2:9372766/140‑1 (MQ=255)

GAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAA‑T‑CATTTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075221‑1075491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`