BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	87.7%	360.8 / 2.3	177	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (13/6); major base A (97/39); minor base T (11/8); total (124/53)
Fisher's exact test for biased strand distribution p-value = 2.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATG  >  NZ_CP025268/1293054‑1293298

gttcagcgTTCCGGTAATTTTGAAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                            <  1:3922243/133‑1 (MQ=11)

  tgagaGTTCAGGGACTTTTGAAAGTGATGTTGGTGGGGGAAGGATTAATCAGCGCTTCGCGCTTCGCCCATCGGGTCGTTTCAATCGGCAACGCACTCTCGCTGGCGCTCGATTCGAACCATGGTCGAAGCTTCtcctcc                                                                                                          >  2:5974209/5‑140 (MQ=21)

           aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCgat                                                                                                 <  2:2183076/140‑3 (MQ=11)

             ggACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCCAAGGGTTCTGGCTGGCGCGCGAGTCGTACCGGGGTCGCGGCGTCTCATCCTTACCCGCGTg                                                                                               >  1:5439908/1‑140 (MQ=2)

                   ctgcaaGTGATGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCTAACCTGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                         <  1:9029054/136‑1 (MQ=11)

                    tGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCTCGCTTGCGCTAGAGTGGTACCTTGGTCGAAGCTTAGCAACCTTACCCCCATggggcgaa                                                                                        >  1:284684/1‑135 (MQ=11)

                                                    aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAG‑AGTTCAgg                                                       <  1:959587/140‑1 (MQ=11)

                                                    aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGGACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTTATTGCGGATTCGTTTGGG‑AGTTCAgg                                                       >  1:7742628/1‑140 (MQ=11)

                                                     gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAggg                                                      <  2:4442422/140‑1 (MQ=21)

                                                     gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTGATTGCGGATTCGCTTGAG‑AGTTCAggg                                                      <  1:6508258/140‑1 (MQ=255)

                                                     gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAggg                                                      <  2:3508420/140‑1 (MQ=21)

                                                     gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAggg                                                      <  1:5055538/140‑1 (MQ=21)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGCATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGa                                                     >  2:7360994/1‑140 (MQ=255)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGG‑AGTTCAGGGa                                                     >  2:1886626/1‑140 (MQ=255)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGa                                                     >  2:7082739/1‑140 (MQ=25)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAG‑AGTTCAGGGa                                                     >  2:5568785/1‑140 (MQ=255)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAAAATTTGATTGCGGATTGGGTTGTG‑AGTTAAGGGa                                                     >  1:11027605/1‑140 (MQ=11)

                                                      cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTCTCATCCATCCCCCCAAGGGCAGCATATTTAATTGGGGTTTAGGTTGGG‑AGTTCAgggc                                                     >  1:7170623/1‑139 (MQ=11)

                                                       gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGAc                                                    <  1:5343788/140‑1 (MQ=255)

                                                       gcTGCGCGCGTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGAc                                                    <  2:11317006/140‑1 (MQ=255)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCGGAATAGTTGATTGCGGATTCGCGTGAG‑AGTTCAGGGACtttt                                                >  2:11478787/1‑140 (MQ=11)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAAGATTTTATTGCGGATTCGGTTGTG‑AGTTCAGGGACtgtt                                                >  2:8898775/1‑140 (MQ=11)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTATTCGAAGCTTATCATACTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGAG‑AGTTCAGGGACtttt                                                >  2:4516743/1‑140 (MQ=11)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACttgt                                                >  2:9484299/1‑138 (MQ=255)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGAG‑AGTTCAGGGACtgtt                                                >  2:9687943/1‑140 (MQ=11)

                                                           cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACtttt                                                <  1:5316963/140‑1 (MQ=255)

                                                            gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTg                                               <  1:10058836/140‑1 (MQ=255)

                                                            gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCCGAATATTTGATTGCGGATTCGGTTGGG‑AGTTCAGGGACTTTTg                                               >  2:864805/1‑140 (MQ=11)

                                                            gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTg                                               <  1:10828833/140‑1 (MQ=255)

                                                            gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTg                                               <  1:8622310/140‑1 (MQ=255)

                                                             cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCTAGCCGCACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTGATTGCGGATTCGCTTGAG‑ACTTCAGGGCCTTTTGa                                              <  1:1989627/140‑1 (MQ=11)

                                                              gcgtCGCCCTTCGGGTCGTTGCCTGCGGCTTCTCTCTGTCGCTGACGCTCGAGCGGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaa                                             <  1:3349209/137‑1 (MQ=11)

                                                              gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaa                                             <  2:7614383/140‑1 (MQ=255)

                                                              gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaa                                             <  2:5051563/140‑1 (MQ=255)

                                                              gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaa                                             <  1:852693/140‑1 (MQ=255)

                                                              gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaa                                             <  1:9825569/140‑1 (MQ=255)

                                                               cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaaa                                            <  1:10834998/140‑1 (MQ=255)

                                                               cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGaaa                                            <  1:7880098/140‑1 (MQ=255)

                                                                ttCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCGGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATACTTACCCGCATGGGCAGAATAATTGATTTCGGATTAGCTTGGG‑AGTTCAGGGTCTTTTTAAAg                                           >  1:6360171/1‑140 (MQ=11)

                                                                 tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAAGTTCAGGGACTTTTGAAAg                                           <  1:2737931/140‑1 (MQ=11)

                                                                   gCCCTTCGTGTCGTTGACTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  2:1098437‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCTAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  2:5025341‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  2:1942563‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  1:7517992‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  1:1448379‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  1:8904225‑M2/1‑138 (MQ=255)

                                                                   gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAGTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTga                                        >  2:3882627‑M2/1‑138 (MQ=255)

                                                                    cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgat                                       >  1:11149885‑M2/1‑137 (MQ=255)

                                                                     ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatg                                      >  2:3286670‑M2/1‑136 (MQ=255)

                                                                     ccTTCGGGTCGTGGCCTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGAGTGCGGCTTCGCGTGAG‑AGTTCAGGGAATATTGCAAGGGAtg                                      <  2:1459910/140‑1 (MQ=11)

                                                                        tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCGGGGGCAGAATTTGTGATTGCGGATTCGTGTGGG‑AGTTCAGGGGCTTTTGAAAGTgttggtg                                   <  2:8275216‑M2/140‑8 (MQ=255)

                                                                         cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtgg                                  <  2:5343580‑M2/140‑9 (MQ=255)

                                                                         cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtgg                                  >  2:7322560‑M2/1‑132 (MQ=255)

                                                                          gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTACTCATCCTTCCCCGCATGGGCAGAATATTAGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggt                                 >  1:10429725‑M2/1‑131 (MQ=255)

                                                                          gggTCGTTGCCTGCGGCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTAGTCGACGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGAATTTTGAAAGTgatggtggt                                 >  2:3233233‑M2/1‑131 (MQ=255)

                                                                             tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggg                              >  2:4791253‑M2/1‑128 (MQ=255)

                                                                             tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggg                              >  1:11446163‑M2/1‑128 (MQ=255)

                                                                               ggtggcTGCGTCCACGCTCTCGCTCTGACGCTCGCCCCGAACCTGCGTCGACGCTTCTCATCCTTCCCCGCTTGGGCAGACTATTTGATTGCGGAGTAGATTGAG‑TGTTCCTGTACTTATGAAAGTGATGGTGGTggggg                            <  1:8416662/135‑1 (MQ=2)

                                                                               ggtgcCTGCGGCAACGCTCTCTCGCTCTCGCTCGAGCCGAACCTTAGTCGTAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGAATTTTGAAAGTgatggtggtggggg                            >  2:6599027‑M2/3‑126 (MQ=255)

                                                                               ggtgcCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAACCTTAGTCGACGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  2:2130046‑M2/3‑126 (MQ=255)

                                                                               ggtgcCTGCGGCAACGCTCTCTCCCTGACGCACGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAANATTTTATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:6166068‑M2/3‑126 (MQ=255)

                                                                               ggtgcCCGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTGCTCATCCTTCCCCGCTTTGGCAGAATATTTGCTTGCGGATACGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  2:7812235‑M2/3‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:1340413‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:711929‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:8220916‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:5159034‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:4895165‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  1:7082739‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  2:1002434‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  2:8470042‑M2/1‑126 (MQ=255)

                                                                               gTTGCCTGCGGCAACGCTCTCTCGCGGTCGCTCCAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtggggg                            >  2:7115210‑M2/1‑126 (MQ=255)

                                                                                 tGCCTGCGGCACCGATCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTGATTGCGGATTAGCTTTAG‑AGTTCAGTGACTTTTGAAAGTgatggtggtgggggaa                          >  1:2068078‑M2/1‑124 (MQ=255)

                                                                                 tGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGTACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTGGATTGCGGATTCGCGGGAG‑AGGTCAGGGACTGTTGGTAGTgatggtggtgggggac                          <  2:7697097‑M2/140‑17 (MQ=255)

                                                                                  gCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaag                         >  2:9059105‑M2/1‑123 (MQ=255)

                                                                                    cTGCGTCAACGCTCTCTCGCTGACGCTCTTGCCGAACCTTTGTCGTAGCTTCTGATCCTTCCCCGATTGGGCAGATTATTTTATTTCGTATTAGCTTGAG‑TGTTCTGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                       <  1:4320238/140‑1 (MQ=11)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGATTATTTGATTGCGGATTAGGTTTAT‑ATTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  1:7730680‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑TGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  1:1778465‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  2:3162780‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  1:4018277‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  1:7425999‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTCGTCGAAGCGTCTCCGCCGTCCCCGCATGGGCAGACTATTTGAGTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGACAGTgatggtggtgggggaagga                       >  2:10281486‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACATTAGTAGATGATTCTCATCCTTCCCCGAATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       >  2:8764892‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCTCTCTCGCTGACGATCTAGCCTAACCTTTTTCGAATCTTATCATCCTTCCCCGCATGGGCAGATTATTTGATTGCGGATTCGCTTGAG‑TTTTCAGTGACTTTTGAAAGTgatggtggtgggggaagga                       >  2:3999388‑M2/1‑121 (MQ=255)

                                                                                    cTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGCACCTGAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAACGTgatggtggtgggggaagga                       >  2:5717363‑M2/1‑121 (MQ=255)

                                                                                      gCGGCAACGCTCTCTCGCTGACGCTCGATCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTTATTGCGGATTCGGTTGAG‑AGTTCAGGGACATTTGAAAGTGAAGGTTGTGGGGGAAGGAtt                     >  2:1751789/1‑140 (MQ=11)

                                                                                      gCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatt                     >  1:8087587‑M2/1‑119 (MQ=255)

                                                                                       cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGTTTGCGGATTCGCTTGTG‑AGTTCCGGGACTTGTGAAAGTgatggtggtgggggaaggatta                    <  2:3686073‑M2/140‑23 (MQ=255)

                                                                                       cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                       <  2:11370572‑M2/137‑20 (MQ=255)

                                                                                       cGGCAACGCTCTCTCGCAGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCCCATGGGCCGAATATGTGATTTCGGATTCGGTTGAG‑AGTTCAGGGACATTTGAAAGTGATGGTGGTGGGGGAAGGAtta                    >  1:10131060/1‑139 (MQ=11)

                                                                                        ggCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGATTGTT‑AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCg                   <  2:1043652/140‑1 (MQ=11)

                                                                                         gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCCTCCGTCCCCGCCTGGGCAGACTATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                  >  1:8139214‑M2/1‑116 (MQ=255)

                                                                                         gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                  >  1:3876256‑M2/1‑116 (MQ=255)

                                                                                             cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc              <  1:4356129‑M2/140‑29 (MQ=255)

                                                                                                tctctcGCTGGCGCTCGAGTCGACCCTTGTTCGAAGCTTCTCTTACTTCACCTCATGGGCAGTATTTTTAATTGCGTATTCGTTGGTT‑TGTTCAGGTAATTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg           <  1:5705748/140‑1 (MQ=2)

                                                                                                  tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagagctgc         <  2:11409888‑M2/140‑34 (MQ=255)

                                                                                                   ctcGCTGTCGCTCTAGGCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCTGATTCGCTTGAG‑ATTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcg        >  2:7793906‑M2/1‑106 (MQ=255)

                                                                                                   ctcGCTGCCGCTCCAGGCGAACCTTCGTCGAAGCGTCTCCTCCTTCCCCGCTGGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGTCTTTTGAAAGTgatggtggggggggaaggattactcagcgctgcg        >  1:318126‑M2/1‑106 (MQ=255)

                                                                                                   ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcg        <  2:9269392‑M2/140‑35 (MQ=255)

                                                                                                     cGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAGTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcg      >  1:10170750‑M2/1‑104 (MQ=255)

                                                                                                        tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGTCTTTTGAACGTgatggtggtgggggaaggattactcagcgctgcgcgctt   >  2:2869913‑M2/1‑101 (MQ=255)

TTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAG‑AGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATG  >  NZ_CP025268/1293054‑1293298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`