Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,623,038 A→G *397* (TAA→TAG)  ydeA → L‑arabinose MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,623,0380AG99.6% 2317.4 / NA 671*397* (TAA→TAG) ydeAL‑arabinose MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (309/357);  minor base T (0/3);  total (310/361)
Fisher's exact test for biased strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 678 total aligned reads displayed.

GTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCC  >  NZ_CP025268/1622901‑1623174
                                                                                                                                         |                                                                                                                                        
gtaggtaATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCACTAGtt                                                                                                                                        >  1:6285037/1‑140 (MQ=255)
  aggtaATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGa                                                                                                                                      <  1:5521208/140‑1 (MQ=255)
  aggtaATCAGGTGAGTTTGCACTGGTCAAGGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTAGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGa                                                                                                                                      <  2:5311786/140‑1 (MQ=255)
   ggtaATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGaa                                                                                                                                     <  2:1479133/140‑1 (MQ=255)
       aTCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGc                                                                                                                                 <  1:8930485/140‑1 (MQ=255)
        tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGcc                                                                                                                                >  1:9694991/1‑140 (MQ=255)
        tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGcc                                                                                                                                >  1:3122221/1‑140 (MQ=255)
        tCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGcc                                                                                                                                >  1:11006736/1‑140 (MQ=255)
          aGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGGTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGTCGCTATAGTTGAAAGGCCCa                                                                                                                              >  2:490755/1‑140 (MQ=255)
           ggTGAGTTAGCACTGGTCAATGTCGATGATTGGTTATGTGGGCCCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCATTGACACTCGAAGAACAGACGCAATAGTTGTAAGGCCCAt                                                                                                                             >  2:6230062/1‑140 (MQ=255)
               aGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTCATTTGGTCAATGATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCACTAGTTGAAAGGCCCagtggg                                                                                                                         >  2:8330986/1‑135 (MQ=255)
               aGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCgg                                                                                                                         <  2:4476540/140‑1 (MQ=255)
                  ttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGcc                                                                                                                      <  2:2587885/140‑1 (MQ=255)
                  ttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGcc                                                                                                                      <  1:6043511/140‑1 (MQ=255)
                  ttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGcc                                                                                                                      <  2:4274863/140‑1 (MQ=255)
                  ttGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGcc                                                                                                                      <  1:10838976/140‑1 (MQ=255)
                   tGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGTACAGACGCAATAGTTGAAAGGCCCATTCGGGCCt                                                                                                                     >  2:2027780/1‑140 (MQ=255)
                         ggTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCTATAGTTGAAAGGCCCAGTCGGGCCTTTTTTa                                                                                                               >  1:2921391/1‑140 (MQ=255)
                         ggTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTa                                                                                                               >  1:942972/1‑140 (MQ=255)
                         ggTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTa                                                                                                               >  1:3457973/1‑140 (MQ=255)
                         ggTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTa                                                                                                               >  1:164547/1‑140 (MQ=255)
                         ggTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTa                                                                                                               >  1:2065031/1‑140 (MQ=255)
                         ggTCAATGACGATGATAGGGTATGTGGGCGCGCTGCCTGCTTTTGCAGCGGTAATTTGGTCCAAAATTAGATGTCGCCGCTGGCCCGTGACACACGAAGAACAGACGCAATAGATGAAAGGCCCATTCGGGCCtttttat                                                                                                               >  2:5951655/1‑138 (MQ=255)
                          gTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                              >  1:7051469/1‑140 (MQ=255)
                          gTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTaa                                                                                                              >  2:8107125/1‑140 (MQ=255)
                              aTGTCGATGATTGGTTATGTGGGCGCTGTGCCTGAGTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAATACCAGCCGCAATAGTTGAAAGGCCCATTCCGGCCTTAATTAATGGt                                                                                                          <  2:8686642/140‑1 (MQ=255)
                                    atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACtttt                                                                                                    >  2:7560155/1‑140 (MQ=255)
                                    atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGttt                                                                                                    >  1:3891067/1‑140 (MQ=255)
                                    atgatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGttt                                                                                                    >  2:1830128/1‑140 (MQ=255)
                                      gatTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTa                                                                                                  <  1:4682299/140‑1 (MQ=255)
                                        ttGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAAt                                                                                                <  1:1063634/140‑1 (MQ=255)
                                                gtgGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCACTAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCa                                                                                        >  2:10102275/1‑140 (MQ=255)
                                                gtgGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCa                                                                                        >  1:4541913/1‑140 (MQ=255)
                                                  gggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAgg                                                                                      <  1:8821284/140‑1 (MQ=255)
                                                   ggCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGa                                                                                     >  1:4775684/1‑140 (MQ=255)
                                                   ggCGAGTTGCCTGATTTTGCCGCGATAAGTAGGTCAATCCTTATAATTCTCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATACTTGAAAGGCCCAGTCGGGACTGATTGAATGGTCCGTTTTCATGCTATCCAGGa                                                                                     >  2:11565793/1‑140 (MQ=255)
                                                     cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCACTAGTTGAAAGGCCCATTCGGTCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                                   >  2:6444652/1‑140 (MQ=255)
                                                     cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                                   >  2:11355695/1‑140 (MQ=255)
                                                     cgcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATg                                                                                   <  1:6831169/140‑1 (MQ=255)
                                                      gcgGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGc                                                                                  >  2:4133293/1‑140 (MQ=255)
                                                         gTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCgg                                                                               >  1:11256508/1‑139 (MQ=255)
                                                         gTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                               <  2:7902861/140‑1 (MQ=255)
                                                         gTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGt                                                                               <  1:4830576/140‑1 (MQ=255)
                                                              tGCTTTTTCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaata                                                                          <  2:1644389/140‑1 (MQ=255)
                                                                cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataat                                                                        <  1:3218678/140‑1 (MQ=255)
                                                                cTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataat                                                                        <  2:7050104/140‑1 (MQ=255)
                                                                 ttttGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataata                                                                       <  1:11610482/140‑1 (MQ=255)
                                                                 tagtGCCGCGTTAATTTGGTCAATCATGATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGATTTAATGATTTCCAGGATGCCGTtaataata                                                                       <  2:9692881/137‑1 (MQ=255)
                                                                    tGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTtaataat                                                                        >  2:5236129/1‑136 (MQ=255)
                                                                    tGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAAc                                                                    >  2:9791763/1‑140 (MQ=255)
                                                                      ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                                  <  1:375269/140‑1 (MQ=255)
                                                                      ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                                  <  2:10775898/140‑1 (MQ=255)
                                                                      ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                                  <  1:10164387/140‑1 (MQ=255)
                                                                      ccGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                                  <  1:10339442/140‑1 (MQ=255)
                                                                      ccGAGTTAATTTGGTCACTCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTg                                                                  <  2:9566611/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTTTCCAGTGACACTCGAAGAACAGACGCAATTGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGCTGCCGTTAATAATAAACTGca                                                                <  2:677822/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  2:5718574/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  1:5838052/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  1:8589804/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  2:7241109/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  1:7416556/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  1:7192650/140‑1 (MQ=255)
                                                                        gcgTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                <  2:9207297/140‑1 (MQ=255)
                                                                          gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                              <  1:9906518/140‑1 (MQ=255)
                                                                          gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                              >  1:6409109/1‑140 (MQ=255)
                                                                          gTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGcaca                                                              >  1:6660361/1‑140 (MQ=255)
                                                                           ttAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                                                             >  1:9150117/1‑140 (MQ=255)
                                                                              aTTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCca                                                          <  1:9231830/140‑1 (MQ=255)
                                                                              aTTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCca                                                          <  1:4018700/140‑1 (MQ=255)
                                                                                     cAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCatacatac                                                   <  2:360334/140‑1 (MQ=255)
                                                                                            cagatTTCGCCCCTCCCCAGTGACACTCGACGCACAGACGCAAAAGTTTAAAGCCCCATCCGGGACTTGTTTAATGGTACCTTTGAATGAATTCCAGGATGCCGTTACTAATAAACTGCACACCCATACATACCAGCAgg                                            <  2:11005216/137‑1 (MQ=255)
                                                                                              tatTGCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTACTGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGaa                                          <  2:2945648/140‑1 (MQ=255)
                                                                                                  tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATccc                                      >  1:2375864/1‑140 (MQ=255)
                                                                                                  tCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAGGTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTGATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCAATACATACCAGCAGGAATcac                                      >  2:5109887/1‑138 (MQ=255)
                                                                                                   cgtcgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa                                     >  2:5496812/4‑140 (MQ=255)
                                                                                                   cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa                                     >  2:3762577/1‑140 (MQ=255)
                                                                                                   cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa                                     >  2:10393369/1‑140 (MQ=255)
                                                                                                   cgccgcTGGCCAGTGACACTCGAAGAACAGACGCAAGAGTTGAAAGGCCCAGTCGGGCCTTTTTTCATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCa                                     >  2:9404654/1‑140 (MQ=255)
                                                                                                    gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCag                                    >  1:10013565/1‑139 (MQ=255)
                                                                                                    gccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCAt                                    <  2:3457973/140‑1 (MQ=255)
                                                                                                     ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATc                                   <  1:4413909/140‑1 (MQ=255)
                                                                                                     ccgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATc                                   >  2:2385751/1‑140 (MQ=255)
                                                                                                      cgcTGGCCAGTGACACTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCacac                                                             >  1:3651248/1‑113 (MQ=255)
                                                                                                                    acTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCt                    <  1:1144041/140‑1 (MQ=255)
                                                                                                                     cTCGAAGAACATACGCAATAGTTGTAAGGCCCATGCGGGACTTTTTTAATGGTACGTTTTAAAGATTTACAGTATGCAGTTAATAATAAACTGCACACCAATACAAACCAGCAGGAATCCCATCAGACGGGCGATCGCtt                   >  2:8882325/1‑140 (MQ=255)
                                                                                                                     cTCGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCtt                   <  2:3280848/140‑1 (MQ=255)
                                                                                                                       cGAAGAACAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCa                 >  1:1242631/1‑140 (MQ=255)
                                                                                                                          agaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATg              >  2:8454641/1‑140 (MQ=255)
                                                                                                                          agaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATg              >  2:4728108/1‑140 (MQ=255)
                                                                                                                           gaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGc             <  1:51833/140‑1 (MQ=255)
                                                                                                                           gaaCAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGc             <  2:8990125/140‑1 (MQ=255)
                                                                                                                              cAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTa                                                                                                         >  2:385693/1‑45 (MQ=255)
                                                                                                                              cAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGca                                                                >  1:4206975/1‑86 (MQ=255)
                                                                                                                              cAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAc          >  1:8165428/1‑140 (MQ=255)
                                                                                                                              cAGACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAc          <  1:8380264/140‑1 (MQ=255)
                                                                                                                                gACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAccc        <  1:2497991/140‑1 (MQ=255)
                                                                                                                                gACGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCAccc        >  1:2649687/1‑140 (MQ=255)
                                                                                                                                 aCGCAATAGTTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCt       <  1:7685825/140‑1 (MQ=255)
                                                                                                                                      atagtTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGccc  <  1:179718/140‑1 (MQ=255)
                                                                                                                                      atagtTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGccc  >  2:10494484/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTCAATCATTATATTTCGCCGCTGGCCAGTGACACTCGAAGAACAGACGCAATAATTGAAAGGCCCATTCGGGCCTTTTTTAATGGTACGTTTTAATGATTTCCAGGATGCCGTTAATAATAAACTGCACACCCATACATACCAGCAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCC  >  NZ_CP025268/1622901‑1623174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑