BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	99.8%	1862.9 / NA	535	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (275/259); minor base A (0/1); total (275/260)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 535 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

  aaGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACg                                                                                                                                       >  2:4604755/1‑140 (MQ=255)

  aaGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACg                                                                                                                                       >  2:5979614/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                    >  1:8600701/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGTATTTATGTTCCTACGTgag‑c                                                                                                                                 >  2:8244672/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                     >  2:2105011/1‑137 (MQ=255)

        gAAAAAAGCCGGAGTGATCAAAAAAAAAGGCGTATATTTGCGATGTGAATGGTTGACAAAATATGAAATAGAATACCTTTTGTCAGCTGACACTTCATCTTATCTTATTGATATAATGTATTTATGTTACTACTTGCG‑c‑c                                                                                                                               >  2:10090500/1‑140 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                        <  2:9738047/140‑6 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccccagcc                                                                                                                        <  1:6493474/140‑6 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCg                                                                                                                       <  2:10610077/140‑1 (MQ=255)

                    aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                    <  1:5532148/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaaca                                                                                                                  <  2:8456081/140‑1 (MQ=255)

                        aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCC‑CCCAGCCCGCAACAAt                                                                                                                <  2:7744936/140‑1 (MQ=255)

                           acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGAt                                                                                                             <  1:5349399/140‑1 (MQ=255)

                             aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCa                                                                                                           <  1:8755815/140‑1 (MQ=255)

                                  aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGATCGCC‑CCCAGCCCGCAACAATGATCAACATg                                                                                                      >  2:8827538/1‑140 (MQ=255)

                                      cGTATATTTGAGCTGTGAATGGTTGACAAAATATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCATACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                  <  1:3184617/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 <  2:5730675/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 >  2:9229732/1‑140 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 >  1:2531714/1‑140 (MQ=255)

                                          tatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAg                                                                                              <  2:2350199/140‑1 (MQ=255)

                                             ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAATATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                           <  2:511194/140‑1 (MQ=255)

                                             ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCACCAATGATCAACATGCCGCACAgcgc                                                                                           >  2:6690018/1‑140 (MQ=255)

                                             ttGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                           <  1:2830734/140‑1 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                  >  1:6933256/1‑126 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                    >  2:2466020/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACTTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  2:2924973/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  1:8365553/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  1:467324/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  2:3994096/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   >  1:3661804/1‑140 (MQ=255)

                                                       aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                 >  2:4741057/1‑140 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                             >  2:349218/1‑140 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                             >  2:4840955/1‑140 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCTGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:1972561/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:1771114/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:6688922/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:2385430/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:8891893/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:5388866/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:5560670/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:6266412/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  2:2749119/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         <  1:10110298/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  1:11499140/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  1:7468394/1‑140 (MQ=255)

                                                                 aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                       <  2:9516967/140‑1 (MQ=255)

                                                                    acaTGAAATAGAATACCTTTTGACAGCTGACACTTCCTCTTATCTTATTGATAAAATGTATTTATGTTCCTACGTGCG‑CACACAGCCCGCAACAATGATCAACATGCCGCAAATCGCAATCAACGCACCCTTCCAGTCAt                                                                    <  2:9094927/138‑1 (MQ=255)

                                                                     gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                   <  2:4462744/140‑1 (MQ=255)

                                                                     gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                   <  1:4840955/140‑1 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                  <  1:10598935/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                             <  1:7696832/140‑1 (MQ=255)

                                                                           aTAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                             <  1:10518354/140‑1 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                        <  1:3420117/140‑1 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                        >  1:9612600/1‑140 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                        >  1:6070415/1‑140 (MQ=255)

                                                                                 tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGt                                                                            <  1:507306/119‑1 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  1:4499054/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  1:7886988/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  1:913215/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  2:4376144/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  1:10195346/1‑140 (MQ=255)

                                                                                         gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                               >  2:5861864/1‑140 (MQ=255)

                                                                                         gTCAGCTGACACTTCCACTTATCTTCGTGTTCCAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCTAAGAGCAATCATAGCACCCGTCCAGTCATAAAGAGTCAGGTTCCCGCCAt                                               >  2:7954603/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAATTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCCCCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcacg                                          >  2:11643294/1‑139 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                          >  2:751737/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGACATCcaca                                          >  2:1549705/1‑140 (MQ=255)

                                                                                                  cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                      <  1:8492455/140‑1 (MQ=255)

                                                                                                   acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                     <  2:4128190/140‑1 (MQ=255)

                                                                                                    cTTCCTCTTATCTTATTGATAAAATGGATTTATGTTACTCCGTGCGCC‑CCCTGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCATTCATAAAGAGTCAGTTTCACGCCATCCACAACgcgc                                    <  1:3013268/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCATTTTCACGCCATCCACAACGCGCAGc                                 <  1:2096793/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:10315040/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:11157158/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:9836536/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:7261286/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:6873031/140‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  2:385425/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  2:3186496/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  1:5964963/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  1:10356522/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  1:1728937/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTCATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  1:7581653/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      <  1:8079657/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  2:6381780/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  1:5798467/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                       <  1:4110790/90‑1 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  1:10046879/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  1:1847311/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  1:9305548/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     <  2:6064067/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                  <  2:7164455/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                  <  2:10867802/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                >  1:5828029/1‑140 (MQ=255)

                                                                                                                           aTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGAc             <  2:1365558/140‑1 (MQ=255)

                                                                                                                                  tctGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCATATCAACGGCGTGCTGACATAAACg      <  2:7596909/138‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:1076501/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:2652591/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:4293916/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:3843545/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:1493458/140‑1 (MQ=255)

                                                                                                                                      ttCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCCACGCCGTGCAGACATAAACGccac  >  2:11306919/1‑140 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`