BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,675,399	A→G	R295G (AGA→GGA)	ynfM →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,675,399	0	A	G	99.5%	2790.7 / NA	803	R295G (AGA→GGA)	ynfM	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (403/396); minor base T (2/2); total (405/398)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 803 total aligned reads displayed.

GCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATT  >  NZ_CP025268/1675263‑1675535

    aTGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCAt                                                                                                                                    >  1:4153982/1‑140 (MQ=255)

       gggTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGac                                                                                                                                 <  1:11096650/140‑1 (MQ=255)

         gTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGacca                                                                                                                               <  1:4044859/140‑1 (MQ=255)

         gTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGacca                                                                                                                               <  2:6231388/140‑1 (MQ=255)

           cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                             >  2:10027051/1‑140 (MQ=255)

           cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                             <  1:1877735/140‑1 (MQ=255)

           cgttcgtCACGCTGTATACTTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                             <  1:7989283/140‑1 (MQ=255)

           cgttcgtCACGATGTTTAATTACATCTTCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATATGACCGGTACATGGAGCTCACCCAAAGCCGCAACCATGaccacc                                                                                                                             <  2:7777818/140‑1 (MQ=255)

           cggtcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                             <  2:5111897/137‑1 (MQ=255)

                 tCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTAt                                                                                                                       <  1:851500/140‑1 (MQ=255)

                 tCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTAt                                                                                                                       <  2:1509022/140‑1 (MQ=255)

                   aCGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATgg                                                                                                                     <  2:211475/140‑1 (MQ=255)

                   aCGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGGACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATgg                                                                                                                     <  2:3077574/140‑1 (MQ=255)

                    cGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATggg                                                                                                                    >  1:2845866/1‑140 (MQ=255)

                      cTGTTTAATTCCATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                  <  1:7243076/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                  <  2:3161249/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTGGGCTTATTATCGCGGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                  <  2:10451851/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGGTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGCCCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCGATGGGCg                                                                                                                  <  1:4477126/140‑1 (MQ=255)

                      cTGTTTAAGGACCTCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                  <  2:11283618/140‑1 (MQ=255)

                      cTGTTTAAATACATCGGCTATCGGTTTATGCTCTCCCCCTGGCATGTCAGACATGCCGTGTTTTGCTTTTTATCGCTGGCTTATTTGTCCGGTACATGGAGCACACCCTAAGCCGGATCCATGACCACCCGCTATGGGCg                                                                                                                  <  2:11419964/140‑1 (MQ=255)

                          ttAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGt                                                                                                              >  1:1706296/1‑140 (MQ=255)

                           tAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                             <  1:6793385/140‑1 (MQ=255)

                            aaTTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTcc                                                                                                            <  1:3919658/140‑1 (MQ=255)

                                 caacGGCTATCTGTTGATGCTCTCACCCTGTAATGTCAGTCAGGCCGTGGTTGGCATATTATCGCTGGCTTATTTGAACGGTACATGGAGCTCAAACAAAGCCGGAACCAAGACAACCAGCTATCGGATGGGTCAAGTGa                                                                                                       >  2:8496009/4‑140 (MQ=255)

                                 cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGa                                                                                                       >  1:2712548/1‑140 (MQ=255)

                                 cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGa                                                                                                       >  2:2854519/1‑140 (MQ=255)

                                 cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAAACATGACCACCCGCTATGTGCGTGGTCCAGTGa                                                                                                       >  2:9181950/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                     >  2:11094888/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                     >  1:9548386/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTAGGGGCGTGGTCCAGTGAtg                                                                                                     >  1:7088034/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACACAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                     >  2:2247130/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCGTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                     >  2:4003593/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGAAGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                     >  2:1178792/1‑140 (MQ=255)

                                        tATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATgttgtt                                                                                                >  2:5815019/1‑140 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTCTCGCTGGCTTATTTGCCCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                          <  2:6476702/140‑1 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                          <  2:1993545/140‑1 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                          <  2:7166187/140‑1 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                          <  1:4605085/140‑1 (MQ=255)

                                                    ctctCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGt                                                                                   >  1:5155454/1‑140 (MQ=255)

                                                      ctcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTa                                                                                 <  1:369487/140‑1 (MQ=255)

                                                      ctcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTa                                                                                 >  1:3442489/1‑140 (MQ=255)

                                                      ctcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTa                                                                                 <  1:323851/140‑1 (MQ=255)

                                                       tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTAt                                                                                >  1:10690990/1‑140 (MQ=255)

                                                       tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTAt                                                                                >  1:2611157/1‑140 (MQ=255)

                                                       tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTAt                                                                                >  1:7348406/1‑140 (MQ=255)

                                                       tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCGATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTAt                                                                                >  1:6526080/1‑140 (MQ=255)

                                                             tGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGtt                                                                          <  1:2174525/140‑1 (MQ=255)

                                                             cggCATGTCCGTCAGGCCGTGGTGGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAACGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGGTGTTTTCGAC‑GGGGGTTATGCTGtt                                                                          <  2:187295/139‑1 (MQ=255)

                                                               gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTg                                                                        >  2:5503530/1‑140 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGttt                                                                    >  1:2978099/1‑140 (MQ=255)

                                                                    tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTa                                                                   >  2:3343325/1‑140 (MQ=255)

                                                                     cagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTAc                                                                  >  1:8837911/1‑140 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACtga                                                               <  2:9582876/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACtga                                                               <  1:10315811/140‑1 (MQ=255)

                                                                          cggCCGTGGTTGGCTTATTATCGCTGGCTTAATTGACCGGGACATGGAGCTCACCCAAAGCCGGAACCCTGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTGACtgatg                                                             <  2:10229735/139‑1 (MQ=255)

                                                                          agGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACtgatg                                                             >  2:8105872/1‑140 (MQ=255)

                                                                              cGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCt                                                         >  1:10029057/1‑140 (MQ=255)

                                                                              cGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCt                                                         >  1:581851/1‑140 (MQ=255)

                                                                                 ggTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTAt                                                      <  2:8981423/140‑1 (MQ=255)

                                                                                  gTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTAtt                                                     >  1:427381/1‑140 (MQ=255)

                                                                                     ggCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAg                                                  <  1:8135673/140‑1 (MQ=255)

                                                                                     ggCTTATTATCGCTGGCTTATTTGACCGGGACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAg                                                  <  2:9745654/140‑1 (MQ=255)

                                                                                       cTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCt                                                <  2:2401796/140‑1 (MQ=255)

                                                                                        ttattaTCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTc                                               >  2:4586290/1‑140 (MQ=255)

                                                                                          attatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                             <  1:1299766/140‑1 (MQ=255)

                                                                                           tgatCGCTGGCTTATTTGACCGGTACCTGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCt                                            <  1:11320920/138‑1 (MQ=255)

                                                                                            tatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                           <  2:7403552/140‑1 (MQ=255)

                                                                                                     cTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATc                                  >  2:10853349/1‑140 (MQ=255)

                                                                                                            gACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                           >  2:3868307/1‑140 (MQ=255)

                                                                                                              ccGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGa                         >  1:1619844/1‑140 (MQ=255)

                                                                                                              acGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGa                         >  2:1673574/2‑140 (MQ=255)

                                                                                                               cGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGaa                        <  1:2486792/140‑1 (MQ=255)

                                                                                                               cGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGaa                        <  1:6341288/140‑1 (MQ=255)

                                                                                                                ggTACATGGAGCTCACCCAAAGCCGGAACCATTACCACCCGCTATGGGCGTGGTCCAGAGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCTCTGAGGCTGATCTTTGCCGGAAt                       >  1:1474835/1‑140 (MQ=255)

                                                                                                                ggTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAAt                       >  1:600762/1‑140 (MQ=255)

                                                                                                                 gTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTGTACTGATGACCTTCTTCAGCTCGCTGTGGCTGATCTTTGCCGGAATg                      <  2:4289216/140‑1 (MQ=255)

                                                                                                                  tACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTATGCCGGAATg                      >  2:6069020/1‑140 (MQ=255)

                                                                                                                    cATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                   >  1:7511896/1‑140 (MQ=255)

                                                                                                                    cATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                   >  2:4698901/1‑140 (MQ=255)

                                                                                                                    cATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                   <  1:10854461/140‑1 (MQ=255)

                                                                                                                    cATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                   >  1:5452667/1‑140 (MQ=255)

                                                                                                                      tGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTAct                 <  1:9732707/140‑1 (MQ=255)

                                                                                                                      tGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTAct                 <  1:4838093/140‑1 (MQ=255)

                                                                                                                       ggAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTActc                <  2:6843544/140‑1 (MQ=255)

                                                                                                                         aGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt              >  2:5347700/1‑140 (MQ=255)

                                                                                                                         aGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt              >  1:11594116/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  1:6981867/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGACCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTGGGGTTTACTGATGACCGTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  2:7917657/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGACTGTTACTCTtctc           >  2:2987054/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:2285089/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:6666964/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:2632531/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:8787623/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:4885379/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:7779568/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTGATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:9341459/1‑140 (MQ=255)

                                                                                                                              aCCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcag         >  1:9035705/1‑140 (MQ=255)

                                                                                                                                ccAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGCTGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcagca       <  2:3543915/140‑1 (MQ=255)

                                                                                                                                ccAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcagca       <  1:3116453/140‑1 (MQ=255)

                                                                                                                                     gCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGAtt  >  1:8336273/1‑140 (MQ=255)

GCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAC‑GGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATT  >  NZ_CP025268/1675263‑1675535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`