BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	160,613	T→C	235 (TAA→TAG)	sfsA ←	DNA/RNA nuclease SfsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	160,613	0	T	C	99.7%	2143.9 / ‑5.0	615	235 (TAA→TAG)	sfsA	DNA/RNA nuclease SfsA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (365/248); minor base A (1/1); total (366/249)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 640 total aligned reads displayed.

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCGG  >  NZ_CP025268/160476‑160749

ggggACAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                        >  1:1043186/1‑140 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                        >  2:5559634/1‑140 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                        <  2:4785114/140‑1 (MQ=255)

ggcgAAATATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                        <  1:10029195/137‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                      <  1:7464652/140‑1 (MQ=255)

    aaaaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAAt                                                                                                                                    >  2:10716866/1‑140 (MQ=255)

            ggCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCgg                                                                                                                            <  1:5960217/140‑1 (MQ=255)

             gCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTAAAATGTAACCGGc                                                                                                                           >  2:7475589/1‑140 (MQ=255)

              ccGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCa                                                                                                                          <  1:5807157/140‑1 (MQ=255)

                gCTATAAATAGCAGAGGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAAGGTAACCGGCAGt                                                                                                                        <  2:7656534/140‑1 (MQ=255)

                   ataAATTGGAGATGGTTTTCCGGAGATCAATTATCTATTCGTTACTCTTGACACCTGTGTGAGGAAAATCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAt                                                                                                                     <  1:7941142/140‑1 (MQ=255)

                   ataAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAt                                                                                                                     <  1:2440540/140‑1 (MQ=255)

                   ataAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAt                                                                                                                     <  1:7716381/140‑1 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                             >  2:5206348/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                             >  2:2724742/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                             >  2:832313/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTAACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                             >  1:10756488/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAAAGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATAAACCAGTTACTTACATTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                             >  2:1948156/1‑140 (MQ=255)

                            aGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAaga                                                                                                            >  2:8526510/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTCTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCTCGCGACCAGAATGTATATTAACCAGTTACTTTCTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  2:4694749/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  1:8479496/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  2:3579410/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  1:660397/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  1:3936477/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTGACTGACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  2:4362378/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAAGTATCTAAACGTAACACTTGACCACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                          >  2:7797273/1‑140 (MQ=255)

                                gCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa                                                                                                        >  2:7965780/1‑140 (MQ=255)

                                   tttCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGc                                                                                                     >  1:1473203/1‑140 (MQ=255)

                                      ccGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCtt                                                                                                  <  1:9888330/140‑1 (MQ=255)

                                            aGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagcag                                                                                            >  1:11361858/1‑140 (MQ=255)

                                                   aTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCATGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                                     >  1:10204293/1‑140 (MQ=255)

                                                     cTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                   <  2:9206931/140‑1 (MQ=255)

                                                     cTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                   <  2:10298903/140‑1 (MQ=255)

                                                              acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                          >  2:1757957/1‑140 (MQ=255)

                                                                acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                        <  1:3825706/140‑1 (MQ=255)

                                                                acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                        <  1:311264/140‑1 (MQ=255)

                                                                  ttGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAg                                                                      <  2:9374223/140‑1 (MQ=255)

                                                                    gACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                                                                    >  1:3620897/1‑140 (MQ=255)

                                                                    gACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                                                                    <  1:8885655/140‑1 (MQ=255)

                                                                    gACAACTGNGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTNACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGaa                                                                    >  1:6186640/1‑140 (MQ=255)

                                                                     aCAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAAt                                                                   <  1:2849476/140‑1 (MQ=255)

                                                                          tgtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                              >  1:7061416/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCCCGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTGTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            >  2:8114103/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  1:3414129/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            >  1:4084599/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  1:11231591/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  1:10978065/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  2:2458676/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  2:2879183/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            >  1:68619/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            <  1:6486479/140‑1 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                            >  1:5494377/1‑140 (MQ=255)

                                                                              tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcccc                                                          >  2:2066641/1‑140 (MQ=255)

                                                                              tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcccc                                                          >  1:1317307/1‑140 (MQ=255)

                                                                               gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                         <  1:9668975/140‑1 (MQ=255)

                                                                               gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                         <  1:7251476/140‑1 (MQ=255)

                                                                                 ggACAAGCGTAGTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCct                                                       >  2:6523306/1‑140 (MQ=255)

                                                                                 ggAAAAGCGTATTTGCGCACGCGACCAGAATGTTAATTAACCAGTTACTTACTTTACGACCATGTAACCGGCAGTGCTTTTTTAAGCGCCATGCCTTCCGCAGAAATTTCCGCATTGTAAGCCAGAATTTCGCCCCCCct                                                       <  2:956238/140‑1 (MQ=255)

                                                                                  gAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCctc                                                      <  1:44072/140‑1 (MQ=255)

                                                                                    aaaGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTg                                                    >  2:10930967/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAACTTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  1:9481404/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  2:11421251/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  1:9270649/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  1:2554222/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  1:2677863/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  >  1:10650722/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                  <  2:8715200/140‑1 (MQ=255)

                                                                                       gCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTg                                                 <  1:5115351/140‑1 (MQ=255)

                                                                                         gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                               <  2:10059298/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                            <  2:9665042/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                            >  1:2113876/1‑140 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                            <  2:4022010/140‑1 (MQ=255)

                                                                                              tGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTTCTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGGTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCt                                          <  2:6315900/140‑1 (MQ=255)

                                                                                                       gACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGt                                 >  1:6347453/1‑140 (MQ=255)

                                                                                                        aCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGtt                                <  1:9015101/140‑1 (MQ=255)

                                                                                                          cAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTgc                              <  1:1671677/140‑1 (MQ=255)

                                                                                                             aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg                           >  2:8175326/1‑140 (MQ=255)

                                                                                                             aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg                           <  2:1331337/140‑1 (MQ=255)

                                                                                                             aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg                           <  2:7175015/140‑1 (MQ=255)

                                                                                                               tGTAAATTAACCAGTTACGTCCTTTACTACAATGTAACCGGCAGTGATGTTTTCAGAGCCATGCCTTCAGCAGAACTTTCAGCTTTGTAAGCCAGAATTTATACCCCCCTCTGTTGAGCGTCGCACAATAGTTGcgcgga                         >  2:8938913/1‑138 (MQ=255)

                                                                                                                gTAAATTAACCAGTTACTTACTTTACTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                        <  2:2306527/140‑1 (MQ=255)

                                                                                                                gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                        <  2:6689406/140‑1 (MQ=255)

                                                                                                                   aaTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtc                     >  1:301061/1‑140 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                               <  2:3045361/113‑1 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtct                    <  1:4914427/140‑1 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTa                                                                            <  2:5936182/81‑1 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                            >  1:8299488/1‑113 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCt                                             <  1:866166/112‑1 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGTCAATAGTTGCGCGTATTTCTCAt                 >  2:11313328/1‑140 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                 >  2:11148766/1‑140 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAAGAGTTGCGCGTATTtctctt                 >  2:6140454/1‑138 (MQ=255)

                                                                                                                        aCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATc                <  1:1895984/140‑1 (MQ=255)

                                                                                                                         ccAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCg               <  2:3247729/140‑1 (MQ=255)

                                                                                                                           aGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAt             >  2:2195847/1‑140 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt           >  2:6845913/1‑140 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  1:4736933/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  2:6568378/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  2:1650253/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  2:3961319/140‑1 (MQ=255)

                                                                                                                                    cttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcgc    <  2:4971589/140‑1 (MQ=255)

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCGG  >  NZ_CP025268/160476‑160749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`