BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	306,029	C→T	V786M (GTG→ATG)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	306,029	0	C	T	98.4%	2255.7 / NA	608	V786M (GTG→ATG)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (298/299); minor base G (3/7); total (301/307)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 613 total aligned reads displayed.

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

    gccCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCAt                                                                                                                                    >  2:11193194/1‑140 (MQ=255)

    gccCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTGTATTGCCACTGTAGCGAAAATCGCTAGTGGGGTATGTCTTATCCATGTCCAt                                                                                                                                    >  2:6289090/1‑140 (MQ=255)

                  gCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTGTTATTGCCACTGTAGCGAAATTCGATCGTGGGGTATGTCTTATCCATGTCCAGGACAAACGCGCGGt                                                                                                                      >  2:6512720/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                  >  2:3932402/1‑140 (MQ=255)

                      cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGGTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTGTc                                                                                                                  >  1:1959552/1‑140 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  2:5617806/140‑1 (MQ=255)

                        gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                <  1:516350/140‑1 (MQ=255)

                            aCCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggt                                                                                                            <  2:5768436/140‑1 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  1:1514013/1‑140 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                       >  2:6194920/1‑140 (MQ=255)

                                 aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTAGCCATGTCCATGACAAACTCGCCGTTTTCTtcgggtcggg                                                                                                       >  2:9531195/1‑140 (MQ=255)

                                  tCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggt                                                                                                      <  1:10894936/140‑1 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     >  1:4408844/1‑140 (MQ=255)

                                   cACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggtt                                                                                                     <  1:11043312/140‑1 (MQ=255)

                                     ccGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgggttcg                                                                                                   <  1:7873313/140‑1 (MQ=255)

                                      cGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGgttcgggttcgg                                                                                                  >  1:4970767/1‑140 (MQ=255)

                                       gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                 <  2:7175899/140‑1 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  2:3365462/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:550151/1‑140 (MQ=255)

                                        aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                >  1:9614506/1‑140 (MQ=255)

                                          ccAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGa                                                                                              >  1:9935037/1‑140 (MQ=255)

                                          ccAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAGAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGGTCGGCCGa                                                                                              >  2:3013104/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                             >  1:1706671/1‑140 (MQ=255)

                                           cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCATATCCATGTCCATGACAAACTCGCCGTTTTCATAGGTTCGGGTTCGGCCGat                                                                                             >  1:8019984/1‑140 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:1162822/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:3874354/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  2:2489571/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:3005981/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:645706/140‑1 (MQ=255)

                                             gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           <  1:11255122/140‑1 (MQ=255)

                                                  cACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttg                                                                                      <  2:3196703/140‑1 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  1:6738594/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                     >  1:7060725/1‑140 (MQ=255)

                                                   aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTGTTCATCGGTTCGGGTTCGGCCGTTATGgttgt                                                                                     >  1:2443093/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:8905480/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  1:3432366/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:11367993/1‑140 (MQ=255)

                                                       cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                 >  2:944965/1‑140 (MQ=255)

                                                        gcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATc                                                                                <  1:10283188/140‑1 (MQ=255)

                                                        gcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATc                                                                                <  1:9393355/140‑1 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  1:10671326/140‑1 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  2:2066493/140‑1 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  2:1567684/140‑1 (MQ=255)

                                                         cgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                               <  2:814298/140‑1 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            <  1:407/140‑1 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            >  1:5365892/1‑140 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            <  2:10676548/140‑1 (MQ=255)

                                                            cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGAAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                            >  2:5027717/1‑140 (MQ=255)

                                                              ggTTGAGTTCCAGAGCCACTTCGCAGNTTTTATTNCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGNTATGGTTGTTAATCCGTGCg                                                                          <  1:6101649/140‑1 (MQ=255)

                                                                ttGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGGTCGGCCGATATGGTTGTTAATCCGTGCGtt                                                                        >  2:4265164/1‑140 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  <  2:7250225/140‑1 (MQ=255)

                                                                      tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTGTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                  >  2:10799969/1‑140 (MQ=255)

                                                                       ccAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagc                                                                 <  2:2275896/140‑1 (MQ=255)

                                                                             ccACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGTGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTACGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGtgtg                                                           <  2:9224664/140‑1 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         >  2:8474361/1‑140 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         >  1:9853148/1‑140 (MQ=255)

                                                                               aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAGAGTGGGGTATTTCTTATCCATGTCCATGACAATCTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                         <  1:10040744/140‑1 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        >  2:2789301/1‑140 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        <  1:8400574/140‑1 (MQ=255)

                                                                                cTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCg                                                        >  2:7421047/1‑140 (MQ=255)

                                                                                    gCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCtt                                                    <  1:7415632/140‑1 (MQ=255)

                                                                                    gCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCtt                                                    <  1:3217629/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  2:7820234/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  1:8843710/140‑1 (MQ=255)

                                                                                        gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                <  2:6016568/140‑1 (MQ=255)

                                                                                         ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                               <  1:3631664/140‑1 (MQ=255)

                                                                                         ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                               <  1:2719948/140‑1 (MQ=255)

                                                                                          agtataGCCACTGTAGCGAAAATCGATAGGGGGGTATTGCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCAc                                              <  2:10481848/138‑1 (MQ=255)

                                                                                             agtGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                           <  2:7614777/138‑1 (MQ=255)

                                                                                             aTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                           <  1:2820279/140‑1 (MQ=255)

                                                                                               tGCCACTGTAGCGAACATCGAGAGTGGGGTAATTCTTACCCATGTCCATGACAAACTCGCCGTTTTCAACGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                         <  2:2162033/140‑1 (MQ=255)

                                                                                                  cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGa                                      >  2:11069543/1‑140 (MQ=255)

                                                                                                  cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGa                                      >  1:2075561/1‑140 (MQ=255)

                                                                                                   aCTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGAc                                     >  1:9099042/1‑140 (MQ=255)

                                                                                                     tGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCg                                   <  1:926210/140‑1 (MQ=255)

                                                                                                         gCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGGTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGgaga                               <  1:6287983/140‑1 (MQ=255)

                                                                                                               aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTaa                         >  2:9684571/1‑140 (MQ=255)

                                                                                                                 agATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAAcc                       <  1:5261886/139‑1 (MQ=255)

                                                                                                                  gagaGTGGGGTATTTCTGATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  2:10036245/137‑1 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  1:1262523/140‑1 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  1:3721311/140‑1 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  2:8646985/140‑1 (MQ=255)

                                                                                                                  gATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCa                      <  1:6015860/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                           >  2:11429361/1‑70 (MQ=255)

                                                                                                                   aTAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  2:5315052/140‑1 (MQ=255)

                                                                                                                   aTAGTGGGGTATATCGACACCATGTCCATGACAACCTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCAt                     <  2:6985524/140‑1 (MQ=255)

                                                                                                                       tGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGGGACGGTAACCATCTGc                 >  2:3825558/1‑140 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCTCGGATACGACCGGAGACGGTAACCATCTGCtt               >  2:10163114/1‑140 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               >  1:2620499/1‑140 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGTCCATGAAAATCTCGCCTATTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               <  1:8233861/140‑1 (MQ=255)

                                                                                                                         gggTATTTCTTATCCATGCCCATCACAAACTCGCCGTTTTCATCGGTTCGGGTTCTGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCtt               >  2:1663745/1‑140 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          >  1:11391877/1‑140 (MQ=255)

                                                                                                                              tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct          >  2:6789617/1‑140 (MQ=255)

                                                                                                                               ttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctc         >  1:8652913/1‑140 (MQ=255)

                                                                                                                                tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct        <  1:2365660/140‑1 (MQ=255)

                                                                                                                                 cTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTg       >  2:4575659/1‑140 (MQ=255)

                                                                                                                                   tATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTACACCTCTGGc     >  2:6666073/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  1:4697043/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  2:3539068/1‑140 (MQ=255)

                                                                                                                                    aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt    >  2:4885926/1‑140 (MQ=255)

CGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCA  >  NZ_CP025268/305892‑306165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`