BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,309,553	A→G	C19C (TGT→TGC)	nlpI ←	lipoprotein NlpI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,309,553	0	A	G	99.8%	2182.2 / NA	627	C19C (TGT→TGC)	nlpI	lipoprotein NlpI
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (370/256); minor base T (0/1); total (370/257)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 634 total aligned reads displayed.

AAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCC  >  NZ_CP025268/3309416‑3309689

aaaaGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCa                                                                                                                                         <  2:8745002/140‑1 (MQ=255)

aaaaGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCa                                                                                                                                         <  2:7358610/140‑1 (MQ=255)

aaaaGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCa                                                                                                                                         <  2:5414293/140‑1 (MQ=255)

aaaaGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCa                                                                                                                                         <  2:3154792/140‑1 (MQ=255)

    gCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCt                                                                                                                                     >  1:1027607/1‑140 (MQ=255)

     cTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACAGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTg                                                                                                                                    <  1:9060580/140‑1 (MQ=255)

      tgtgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGc                                                                                                                                   <  2:11018104/140‑1 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGANTCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  1:6177630/1‑140 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCGCTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  1:2756376/1‑140 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  2:10180065/1‑140 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  1:3220208/1‑140 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  1:2582791/1‑140 (MQ=255)

        tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCaa                                                                                                                                 >  1:171556/1‑140 (MQ=255)

          cgcgTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGc                                                                                                                               >  1:7386943/1‑140 (MQ=255)

          cgcgTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGc                                                                                                                               <  2:8911605/140‑1 (MQ=255)

               tCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAg                                                                                                                          >  2:5503909/1‑140 (MQ=255)

               tCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAg                                                                                                                          >  1:2919659/1‑140 (MQ=255)

               tCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAg                                                                                                                          >  2:8173321/1‑140 (MQ=255)

                  tCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGc                                                                                                                       >  2:9632091/1‑140 (MQ=255)

                  tCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGc                                                                                                                       >  1:6673074/1‑140 (MQ=255)

                  tCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGGATTACTGCATCCTGCAAGCGTAAGTGc                                                                                                                       >  1:5358332/1‑140 (MQ=255)

                       ggTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCg                                                                                                                  >  2:9323612/1‑140 (MQ=255)

                       ggTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCg                                                                                                                  <  2:6769310/140‑1 (MQ=255)

                        gTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                                 <  2:11286745/140‑1 (MQ=255)

                        gTTAAAGCCCGACTGGCAAGGATTTGCTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                                 <  2:1368918/140‑1 (MQ=255)

                           aaaGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAAc                                                                                                              >  2:4410996/1‑140 (MQ=255)

                                 cGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAaca                                                                                                        >  2:7761558/1‑140 (MQ=255)

                                 cGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAaca                                                                                                        >  2:3962491/1‑140 (MQ=255)

                                   aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGACACAcc                                                                                                      >  1:1204000/1‑140 (MQ=255)

                                   aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACTcc                                                                                                      >  1:4983453/1‑140 (MQ=255)

                                   aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACAcc                                                                                                      >  1:3686729/1‑140 (MQ=255)

                                   aCTGGCAAGGAGTTGTTCCAGACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACAcc                                                                                                      >  1:6084240/1‑140 (MQ=255)

                                      ggCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAgc                                                                                                   <  1:2128093/140‑1 (MQ=255)

                                                      aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCAt                                                                                  >  2:4229637/1‑140 (MQ=255)

                                                      aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCAt                                                                                  >  1:926523/1‑140 (MQ=255)

                                                      aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCAt                                                                                  >  1:2415377/1‑140 (MQ=255)

                                                      aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCAt                                                                                  >  1:4588249/1‑140 (MQ=255)

                                                       tACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCAGGTTTACGCCAGGAAGTATTAATGCATACTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGAGCAC‑AAAAGGCGTCagg                                                                                 >  2:6613947/1‑138 (MQ=255)

                                                       tACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAC‑AAAAGGCTTCAtt                                                                                 >  2:6887178/1‑140 (MQ=255)

                                                       tACGGGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCCAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAATAAAAGGCtaaat                                                                                  >  2:2244248/1‑136 (MQ=255)

                                                          gTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTcc                                                                              <  1:6892656/140‑1 (MQ=255)

                                                          gTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTcc                                                                              <  1:7087392/140‑1 (MQ=255)

                                                            gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCa                                                                            <  2:7061480/140‑1 (MQ=255)

                                                            gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATGTCCCa                                                                            >  2:2960547/1‑140 (MQ=255)

                                                             ccAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCAc                                                                           <  1:9817221/140‑1 (MQ=255)

                                                                  aTCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCg                                                                      <  1:2984787/140‑1 (MQ=255)

                                                                  aTCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCg                                                                      <  2:786680/140‑1 (MQ=255)

                                                                    cACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGaa                                                                    <  1:1589980/140‑1 (MQ=255)

                                                                     aCTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAg                                                                   >  1:1000480/1‑140 (MQ=255)

                                                                      cTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGa                                                                  >  2:8004808/1‑140 (MQ=255)

                                                                      cTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGa                                                                  >  2:6515039/1‑140 (MQ=255)

                                                                       ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGAc                                                                 >  1:11003287/1‑140 (MQ=255)

                                                                       ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGAc                                                                 <  2:4729018/140‑1 (MQ=255)

                                                                       ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGC‑A‑AAAAGGCTTCATTTCCCACTCCCGAAGAcc                                                                <  1:11390260/140‑1 (MQ=255)

                                                                        tCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTACACTTGTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGAcc                                                                <  2:7519158/140‑1 (MQ=255)

                                                                         ccTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCa                                                               <  1:8914364/140‑1 (MQ=255)

                                                                         ccTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCa                                                               <  1:3432621/140‑1 (MQ=255)

                                                                         ccTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCa                                                               >  1:10110799/1‑140 (MQ=255)

                                                                           tgctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACg                                                             >  2:5304514/1‑140 (MQ=255)

                                                                            gctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACgg                                                            <  1:11453281/140‑1 (MQ=255)

                                                                               gTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtg                                                         >  1:867762/1‑140 (MQ=255)

                                                                               gTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATGTCCCACTCCCGAAGACCACGGTtg                                                         <  2:1702440/140‑1 (MQ=255)

                                                                                 aaaGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                       >  1:10926233/1‑140 (MQ=255)

                                                                                 aaaGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                       <  2:10721385/140‑1 (MQ=255)

                                                                                 aaaGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                       <  2:2605338/140‑1 (MQ=255)

                                                                                 aaaGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                       <  1:3919025/140‑1 (MQ=255)

                                                                                     tCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaatgaa                                                   <  1:10670377/140‑1 (MQ=255)

                                                                                        gTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGt                                                >  2:3308281/1‑140 (MQ=255)

                                                                                        gTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGt                                                >  2:411560/1‑140 (MQ=255)

                                                                                        gTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGt                                                >  2:5832765/1‑140 (MQ=255)

                                                                                              aTGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGtt                                          >  1:1378702/1‑140 (MQ=255)

                                                                                                ggTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTcc                                        <  2:841227/140‑1 (MQ=255)

                                                                                                ggTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTcc                                        <  2:5562641/140‑1 (MQ=255)

                                                                                                 gTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTccc                                       >  1:7591773/1‑140 (MQ=255)

                                                                                                   aCCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGAAAGCGTAAGTGCTGTCGAAACGAAAAACCAGCGCAA‑AAAAGGCTTCATTTCACACTCCCGAAGACAACGGTTGAATGAACGTCCTGTTCCCgg                                     >  2:7618660/1‑140 (MQ=255)

                                                                                                      gcgAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTg                                  >  2:6037104/1‑140 (MQ=255)

                                                                                                      gcgAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTg                                  >  1:5078874/1‑140 (MQ=255)

                                                                                                       cgAGGACTTCACTTTTTCGCGAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGc                                 >  2:4970032/1‑140 (MQ=255)

                                                                                                       cgAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑TAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGc                                 >  1:3131626/1‑140 (MQ=255)

                                                                                                       cgAGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGc                                 >  1:5583118/1‑140 (MQ=255)

                                                                                                        gAGGACGTCACTTTTACGCCAGGAAGTAGTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCt                                <  2:8498594/140‑1 (MQ=255)

                                                                                                         aGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTa                               >  1:8224185/1‑140 (MQ=255)

                                                                                                         aGGACTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTa                               >  1:853009/1‑140 (MQ=255)

                                                                                                         aGGACTTCACTGTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCtc                               >  2:5670502/1‑139 (MQ=255)

                                                                                                         aGGACTTCACGTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGACGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTa                               >  2:2798231/1‑140 (MQ=255)

                                                                                                            aCTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACa                            >  1:4318718/1‑140 (MQ=255)

                                                                                                             cTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACaa                           <  1:6369060/140‑1 (MQ=255)

                                                                                                             cTTCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACaa                           <  1:4042736/140‑1 (MQ=255)

                                                                                                              gtCACTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAg                          <  1:4765802/139‑1 (MQ=255)

                                                                                                                 aCTTTTACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCg                       >  1:5169568/1‑140 (MQ=255)

                                                                                                                   ttttACGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTc                     <  2:3692986/140‑1 (MQ=255)

                                                                                                                      tACGCCAGGAACTATTACTGCATCCTGAAAGAGTAAGTGCTGTCGCAAACAAAAACCAGCGAAC‑AAAAGGATTCATATCCCAATCCAGAATAACACGGTTGAATCAACGGCCTGTTACCGGTTGCTAACAAGGCGTcatc                  >  2:9088957/1‑137 (MQ=255)

                                                                                                                        cGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc                <  2:6201451/140‑1 (MQ=255)

                                                                                                                        cGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc                >  1:7978952/1‑140 (MQ=255)

                                                                                                                        cGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc                <  2:10452552/140‑1 (MQ=255)

                                                                                                                        cGCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc                >  2:1872504/1‑140 (MQ=255)

                                                                                                                         gCCAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGccc               <  1:9451558/140‑1 (MQ=255)

                                                                                                                          ccAGGAAGTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCg              <  2:376919/140‑1 (MQ=255)

                                                                                                                                  tATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAg      <  2:1183976/140‑1 (MQ=255)

                                                                                                                                   aTTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGc     >  1:5926051/1‑140 (MQ=255)

AAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAA‑AAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCC  >  NZ_CP025268/3309416‑3309689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`