BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	339,140	A→G	99 (TAA→TAG)	CXP41_RS01780 →	protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	339,140	0	A	G	99.8%	2031.1 / NA	601	99 (TAA→TAG)	CXP41_RS01780	protein YahH
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (268/331); total (270/331)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

GGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGC  >  NZ_CP025268/339003‑339275

   gTGGACGCCTTATCCGGCCTACGAGTGGCGCGCGCATCTGTAGGCCTGATAAGCGTAGCGCAACAGGCATTTGTCACCCTTGCCGGATGCGGCGTGAACGCCTTCTCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTg                                                                                                                                    <  2:6006930/140‑1 (MQ=14)

    tGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGa                                                                                                                                   <  2:2762328/140‑1 (MQ=255)

     ggACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGAt                                                                                                                                  <  1:3584684/140‑1 (MQ=255)

      gACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATa                                                                                                                                 <  2:9441503/140‑1 (MQ=255)

          ccTTCTCCGGCCCACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCCCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCg                                                                                                                             <  1:4390237/140‑1 (MQ=255)

           cTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGt                                                                                                                            >  2:4277212/1‑140 (MQ=255)

           cTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGt                                                                                                                            <  2:5929369/140‑1 (MQ=255)

              aTCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAgc                                                                                                                         >  2:2209855/1‑140 (MQ=255)

              aTCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAgc                                                                                                                         >  1:8014802/1‑140 (MQ=255)

                   gCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATc                                                                                                                    <  1:8377360/140‑1 (MQ=255)

                   gCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGGGTAT‑TGTAGGCCTGATAAGCGGAGCGCATc                                                                                                                    >  2:1521121/1‑140 (MQ=255)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                  >  1:1588600/1‑140 (MQ=255)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                  >  1:6312647/1‑140 (MQ=255)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGACGGATGCTGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                  >  2:7214837/1‑140 (MQ=255)

                      tACGAGTGGCGCTAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                 <  1:5985650/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                 <  1:8972534/140‑1 (MQ=255)

                      tACGAGTGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                 <  2:2004158/140‑1 (MQ=255)

                      aaCGAGTGGCGCGAGCCTCGGTAGGGCTGATTTGCGTAGCGCATCAGGCCTTTGTCCCCCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                 <  2:804448/139‑1 (MQ=14)

                             ggCGCGAGAATCTTTAGGCCTGATAAGCGTAGCGCATCAGGCAATTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                          <  2:2491593/140‑1 (MQ=255)

                             ggCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                          >  1:6257933/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                         >  2:8928226/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                         >  1:3755991/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                         >  2:8731211/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCAGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                         >  1:10418217/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGGCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                         >  2:733283/1‑140 (MQ=255)

                                gcaagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                       <  2:552158/137‑1 (MQ=255)

                                 cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                      <  1:3441586/140‑1 (MQ=255)

                                 cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                      <  1:8497676/140‑1 (MQ=255)

                                 agagtATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                      <  1:1365813/139‑1 (MQ=255)

                                  tagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGtt                                                                                                     <  1:1498789/139‑1 (MQ=255)

                                   agaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGACT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTg                                                                                                    >  2:6688176/1‑140 (MQ=255)

                                   agaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTg                                                                                                    >  1:2465275/1‑140 (MQ=255)

                                        cTGTTGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATGGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                               <  2:350800/140‑1 (MQ=255)

                                        cTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                               <  1:8752535/140‑1 (MQ=255)

                                         tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGACT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGGCATa                                                                                              >  1:10709031/1‑140 (MQ=255)

                                          gTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATaa                                                                                             <  1:2167250/140‑1 (MQ=255)

                                            aGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcc                                                                                           <  2:11553627/140‑1 (MQ=255)

                                            aGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcc                                                                                           <  2:10946395/140‑1 (MQ=255)

                                               ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGTTGCGGCGTGAACGCCTTATCCGGCCTACT‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                        <  2:9640160/140‑1 (MQ=255)

                                               agtgttAATCGTAGCGCATCATGCATTTGTGACGATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                        <  1:5425304/135‑1 (MQ=17)

                                                 tgtctaGCGTTGCGGATCAGGAAATTGTCCCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                      <  2:11401088/135‑1 (MQ=18)

                                                 tGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                      <  1:4347348/140‑1 (MQ=255)

                                                 tGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                      <  1:8141133/140‑1 (MQ=255)

                                                 tGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                      <  1:4084029/140‑1 (MQ=255)

                                                  gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                 <  2:1188855/112‑1 (MQ=38)

                                                  gATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑GGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCa                                                                                     >  2:5382408/1‑140 (MQ=255)

                                                   aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAc                                                                                    >  1:7834642/1‑140 (MQ=255)

                                                    tAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                                   <  1:6891470/140‑1 (MQ=255)

                                                      aGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                                 <  2:3318732/140‑1 (MQ=255)

                                                        cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCGACG‑AGTGGCGCGAGACT‑GGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCcgct                                                                               >  1:2325117/1‑140 (MQ=18)

                                                          tAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCTCCCTCTGt                                                                             >  1:2021891/1‑140 (MQ=255)

                                                           aGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                            <  1:11644471/140‑1 (MQ=255)

                                                           aGCGCATCAGGAATTTGTCACCCTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                            <  2:10337217/140‑1 (MQ=255)

                                                            gcgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAG‑TGTAGGCCTGATAAGCGTATCGCATCAGGCAGTCTGGCGTTGGTCATATCCCCATCACCATCGGTAg                                                                           >  2:9863212/1‑140 (MQ=18)

                                                             cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGc                                                                          >  1:9811299/1‑140 (MQ=255)

                                                             cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGc                                                                          >  1:270797/1‑140 (MQ=255)

                                                                aTCAGGCATTTGTCCCCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGa                                                                       <  1:6596034/140‑1 (MQ=255)

                                                                aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCggt                                                                       >  2:8297767/1‑139 (MQ=255)

                                                                aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGa                                                                       >  1:7376906/1‑140 (MQ=255)

                                                                   aGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                                                    <  1:9448206/140‑1 (MQ=255)

                                                                   aGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACAt                                                                    <  2:1124900/140‑1 (MQ=255)

                                                                    ggCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATa                                                                   >  1:4764233/1‑140 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATag                                                                  <  1:11434930/140‑2 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                  <  2:4692056/140‑1 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                  <  1:2922950/140‑1 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                  <  2:7838833/140‑1 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                  <  1:9165232/140‑1 (MQ=255)

                                                                        tttGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaa                                                               <  1:3907232/140‑1 (MQ=255)

                                                                                acatGCCGGTTGCGGCGTGCACGCCTTATCCGGCCTACGTAGT‑GCGCATTTATATGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGaaa                                                        <  2:86100/137‑1 (MQ=18)

                                                                                 aTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑ATTGGCGAGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAaga                                                      <  1:1811783/140‑1 (MQ=255)

                                                                                 aTTGCCGCATGCGGCGTGAACCCCTTATCCGGCCTACG‑AGAGGCGCGTTATT‑TGTAGGCCGGCTCAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAaga                                                      <  2:10769195/140‑1 (MQ=37)

                                                                                  ttGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAagag                                                     >  1:10049798/1‑140 (MQ=255)

                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑TGTGGCGCGAGAAT‑TGTAGGCCTGTTAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAagagg                                                    >  2:7465578/1‑139 (MQ=255)

                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGc                                                    >  1:11232755/1‑140 (MQ=255)

                                                                                         aTGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTgg                                              <  1:7252235/140‑1 (MQ=255)

                                                                                          tGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                                             >  1:1011914/1‑140 (MQ=255)

                                                                                          tGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                                             >  1:5833347/1‑140 (MQ=255)

                                                                                           gcggcgTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTc                                            <  2:9261592/140‑1 (MQ=255)

                                                                                           gcggcgTGAACGACTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTc                                            <  1:4975142/140‑1 (MQ=255)

                                                                                                  gAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa                                     <  2:10538383/140‑1 (MQ=255)

                                                                                                  gAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa                                     <  2:64726/140‑1 (MQ=255)

                                                                                                  gAACGCATTTCCCGGCCACCT‑AGGGGGGGGAGAAA‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCa                                     <  2:5034725/140‑1 (MQ=37)

                                                                                                   aaCGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCAt                                    >  1:7176430/1‑140 (MQ=255)

                                                                                                    aCGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCAtt                                   >  1:11563346/1‑140 (MQ=255)

                                                                                                      gcattATCCGGCCTACG‑CGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtgg                                 <  1:7630844/137‑1 (MQ=255)

                                                                                                               ggCCTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAg                        <  2:3024867/140‑1 (MQ=255)

                                                                                                                 ccTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                      <  1:670383/140‑1 (MQ=255)

                                                                                                                 ccTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                      <  2:1391269/140‑1 (MQ=255)

                                                                                                                 ccTACG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                      <  1:10536263/140‑1 (MQ=255)

                                                                                                                 ccTAAG‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTTGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                      <  1:4579774/140‑1 (MQ=255)

                                                                                                                      g‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATc                 <  2:10425093/140‑1 (MQ=255)

                                                                                                                      g‑AGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATc                 <  1:5206019/140‑1 (MQ=255)

                                                                                                                        aGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGACAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCa                <  2:3824604/140‑1 (MQ=255)

                                                                                                                            gcgcGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa            >  1:10875927/1‑140 (MQ=255)

                                                                                                                            gcgcGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa            >  1:5752268/1‑140 (MQ=255)

                                                                                                                            gcgcGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa            >  2:10069524/1‑140 (MQ=255)

                                                                                                                            gcgcGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAACCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa            >  2:6968396/1‑140 (MQ=255)

                                                                                                                            gcgcGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTGGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa            >  2:11078868/1‑140 (MQ=255)

                                                                                                                                gagaAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAAGATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTCTCAAAGAttcc        >  2:9201630/1‑137 (MQ=255)

                                                                                                                                   aaT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACgcc     <  2:7650360/140‑1 (MQ=255)

GGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACG‑AGTGGCGCGAGAAT‑TGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGC  >  NZ_CP025268/339003‑339275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`