BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,386,542	T→C	91 (TAA→TAG)	yhcO ←	barstar family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,386,542	0	T	C	99.7%	1022.2 / NA	295	91 (TAA→TAG)	yhcO	barstar family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (180/114); minor base A (1/0); total (181/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

AGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NZ_CP025268/3386405‑3386675

agagAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTa                                                                                                                                     <  1:9243483/140‑1 (MQ=255)

      aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACg                                                                                                                               >  1:1666714/1‑140 (MQ=255)

      aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACg                                                                                                                               <  2:2663407/140‑1 (MQ=255)

         cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGTCGATTTTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGTCGAAc                                                                                                                            >  1:9518645/1‑140 (MQ=255)

         cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAAc                                                                                                                            >  2:2056190/1‑140 (MQ=255)

         cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAAc                                                                                                                            >  2:7767833/1‑140 (MQ=255)

           cAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACa                                                                                                                           >  2:10473706/1‑140 (MQ=255)

           cAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACAt                                                                                                                          >  2:9137901/1‑140 (MQ=255)

            aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACAtt                                                                                                                         >  2:7863134/1‑140 (MQ=255)

            aaCGGCCTACCAGAGTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑‑TTTTTTTGCGCTAATGACGAACATTa                                                                                                                        >  1:5471073/1‑140 (MQ=255)

              cGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                       <  1:2325034/140‑1 (MQ=255)

                gCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTaaaa                                                                                                                     <  1:5619001/140‑1 (MQ=255)

                   tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGc                                                                                                                  >  1:11166267/1‑140 (MQ=255)

                   tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGc                                                                                                                  >  2:6194507/1‑140 (MQ=255)

                       aGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAAt                                                                                                              <  1:9099129/140‑1 (MQ=255)

                          ttACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGcc                                                                                                           >  1:11440295/1‑140 (MQ=255)

                          ttACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGcc                                                                                                           >  1:8735849/1‑140 (MQ=255)

                           tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                          >  1:7990161/1‑140 (MQ=255)

                           tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                          >  1:9700155/1‑140 (MQ=255)

                           tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                          >  2:3568537/1‑140 (MQ=255)

                             cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                        >  1:4259032/1‑140 (MQ=255)

                             cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                                                        >  1:10893983/1‑140 (MQ=255)

                               gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                      <  1:4737947/140‑1 (MQ=255)

                               gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                      <  1:6086901/140‑1 (MQ=255)

                                aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                                     >  1:4928364/1‑140 (MQ=255)

                                aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                                     >  1:5379241/1‑140 (MQ=255)

                                   cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  >  1:2968235/1‑140 (MQ=255)

                                   cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  >  2:5603878/1‑140 (MQ=255)

                                   cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  <  2:7996196/140‑1 (MQ=255)

                                   cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGCCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                  >  1:1220217/1‑140 (MQ=255)

                                    tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                                 <  1:6550222/140‑1 (MQ=255)

                                     cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                                >  2:2214988/1‑140 (MQ=255)

                                       tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATCTTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                              >  1:6017448/1‑140 (MQ=255)

                                       tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCtt                                                                                               >  2:2811045/1‑140 (MQ=255)

                                       tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                              >  1:509476/1‑140 (MQ=255)

                                        aGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                                             >  2:631243/1‑140 (MQ=255)

                                         gCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                            <  1:7132071/140‑1 (MQ=255)

                                         gCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                            <  1:770817/140‑1 (MQ=255)

                                            gTGACCCCGGGCTCGCTACGGCTGAACTGTACAAATAAACCCTCAGCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGTC‑TTTATTTTGCGCTAATGACGCACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                         <  2:10243679/140‑1 (MQ=255)

                                               acacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTGTTTTTGTGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                      >  1:10520778/1‑140 (MQ=255)

                                                cacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtc                                                                                     >  2:759788/1‑140 (MQ=255)

                                                 acCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                                                    <  1:2729597/140‑1 (MQ=255)

                                                    tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                 <  2:9522656/140‑1 (MQ=255)

                                                    tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                 <  2:4572855/140‑1 (MQ=255)

                                                    tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                 <  2:8447929/140‑1 (MQ=255)

                                                     ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                 >  1:58613/1‑140 (MQ=255)

                                                     ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                                >  1:7327990/1‑140 (MQ=255)

                                                     ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                                <  1:3876527/140‑1 (MQ=255)

                                                       cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                                              <  2:9848620/140‑1 (MQ=255)

                                                         cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                            <  2:8841636/140‑1 (MQ=255)

                                                           cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                          >  1:6239176/1‑140 (MQ=255)

                                                           cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                          >  1:8172686/1‑140 (MQ=255)

                                                                  tGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                                   <  1:7429390/140‑1 (MQ=255)

                                                                    caCTGTACAAATAAACCCTCATCGACTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAAAATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                 >  2:1034346/2‑140 (MQ=255)

                                                                    aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                                             <  2:10741937/112‑1 (MQ=255)

                                                                    aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                 >  2:8221578/1‑140 (MQ=255)

                                                                    aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                 >  1:6940765/1‑140 (MQ=255)

                                                                    aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                                 >  1:4029079/1‑140 (MQ=255)

                                                                        gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGcc                                                             >  1:3238434/1‑140 (MQ=255)

                                                                         tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                            >  2:1325137/1‑140 (MQ=255)

                                                                         tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                            >  2:9283236/1‑140 (MQ=255)

                                                                         tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCACTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                            >  2:4798953/1‑140 (MQ=255)

                                                                                aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAATGGc                                                     <  2:10057326/140‑1 (MQ=255)

                                                                                aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGc                                                     <  2:4029079/140‑1 (MQ=255)

                                                                                  aCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAACCAGCAATATTAACGCGCCAAAACGGCGa                                                   <  1:8529499/140‑1 (MQ=255)

                                                                                  aCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa                                                   <  1:5931087/140‑1 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTACTGACGCACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCGGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCgtc                                                  >  1:430124/1‑138 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                  >  1:8378888/1‑140 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                  >  1:1955262/1‑140 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                  >  1:2836032/1‑140 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                  >  1:6345847/1‑140 (MQ=255)

                                                                                   cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTACCAGCTCTACCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAAAGGCGAc                                                  >  2:7131751/1‑140 (MQ=255)

                                                                                        aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                                             >  2:6636296/1‑140 (MQ=255)

                                                                                        aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                                             >  2:6559872/1‑140 (MQ=255)

                                                                                              ttGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                       >  2:3355431/1‑140 (MQ=255)

                                                                                              ttGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                       >  1:4516662/1‑140 (MQ=255)

                                                                                              ttGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                       <  1:1414458/140‑1 (MQ=255)

                                                                                              gtGTCCGACGATAGTGCCCCCGGTTCGGGGGC‑TATTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                       <  1:3431621/139‑1 (MQ=255)

                                                                                               tGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctctc                                      <  1:1486720/140‑1 (MQ=255)

                                                                                                gTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc                                     >  1:2857012/1‑140 (MQ=255)

                                                                                                 tCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTccc                                    >  1:10948336/1‑140 (MQ=255)

                                                                                                    gacgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGa                                 <  1:8333752/140‑1 (MQ=255)

                                                                                                     acgaTATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGAt                                >  1:6999722/1‑140 (MQ=255)

                                                                                                           ttGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                          >  2:3177761/1‑140 (MQ=255)

                                                                                                              cccccGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAAtt                       <  1:10035966/140‑1 (MQ=255)

                                                                                                              cccccGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAAtt                       <  2:10539572/140‑1 (MQ=255)

                                                                                                                  cGGTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACCTTAAAACGCAAATGCCCTTCCCGCTCGTCCTCTGCCTCATCACACAGCAATATTAACGCGCCAAAACGGCGACGCGGTTTCTCTCCCAGATGAACAAATTCAAt                   <  2:11347198/140‑1 (MQ=255)

                                                                                                                   ggTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                            >  2:1091688/1‑82 (MQ=255)

                                                                                                                   ggTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                  >  2:11611970/1‑140 (MQ=255)

                                                                                                                   ggTTCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                  >  2:4647328/1‑140 (MQ=255)

                                                                                                                     ttCGGGGGC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtctc                <  1:7094584/140‑1 (MQ=255)

                                                                                                                       cGGGGGC‑TATTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCaa              <  2:6135949/140‑1 (MQ=255)

                                                                                                                        gggggC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAg             >  1:2595329/1‑140 (MQ=255)

                                                                                                                            gC‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt         >  1:2479424/1‑140 (MQ=255)

                                                                                                                             c‑TTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTa        >  1:10725111/1‑140 (MQ=255)

                                                                                                                               ttttatttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAg       <  2:8762348/140‑1 (MQ=255)

                                                                                                                                tttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGc      >  2:5968959/1‑140 (MQ=255)

                                                                                                                                tttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGc      >  2:2594132/1‑140 (MQ=255)

                                                                                                                                    tttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCa  <  1:10193337/140‑1 (MQ=255)

                                                                                                                                    tttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCa  <  2:6405216/140‑1 (MQ=255)

AGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑TTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGGCA  >  NZ_CP025268/3386405‑3386675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`