BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,504,638	A→G	262 (TAA→TAG)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,504,638	0	A	G	98.8%	1786.8 / NA	516	262 (TAA→TAG)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (188/322); minor base T (0/6); total (188/328)
Fisher's exact test for biased strand distribution p-value = 9.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 535 total aligned reads displayed.

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTCT  >  NZ_CP025268/3504501‑3504774

 tCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTggtaggta                                                                                                                                       <  2:4460216/140‑1 (MQ=255)

      acCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACg                                                                                                                                  >  1:10913333/1‑140 (MQ=255)

      acCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACg                                                                                                                                  <  1:11272517/140‑1 (MQ=255)

      acCATGGGTGCCGGAGATTCGTTCAGTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACg                                                                                                                                  <  2:3538748/140‑1 (MQ=255)

       ccATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGt                                                                                                                                 >  1:5708477/1‑140 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                                >  1:5155140/1‑140 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                                >  1:7414785/1‑140 (MQ=255)

        cATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGtt                                                                                                                                <  2:8720506/140‑1 (MQ=255)

          tGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTgg                                                                                                                              >  1:4603346/1‑140 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                             <  2:2676543/140‑1 (MQ=255)

           gggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGc                                                                                                                             <  1:9075823/140‑1 (MQ=255)

            ggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCg                                                                                                                            >  1:10003266/1‑140 (MQ=255)

            ggTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCg                                                                                                                            <  2:6023508/140‑1 (MQ=255)

                ccGGAGATTCGTTCAGTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAg                                                                                                                        <  2:4873465/140‑1 (MQ=255)

                 cGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGc                                                                                                                       <  1:11613415/140‑1 (MQ=255)

                        tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                                >  1:123634/1‑140 (MQ=255)

                        tCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCa                                                                                                                <  2:5754545/140‑1 (MQ=255)

                          ggtCATTGCCGGATTCAATTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                              <  2:774862/138‑1 (MQ=255)

                          gTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACg                                                                                                              <  1:2593587/140‑1 (MQ=255)

                           ttCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGc                                                                                                             <  2:2469966/140‑1 (MQ=255)

                             cagtGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                           <  2:6883836/137‑1 (MQ=255)

                             cATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                           <  2:10986935/140‑1 (MQ=255)

                             cATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCa                                                                                                           <  2:3353886/140‑1 (MQ=255)

                               ttGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAAc                                                                                                         <  2:5489770/140‑1 (MQ=255)

                                tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACg                                                                                                        >  1:1807715/1‑140 (MQ=255)

                                tGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGAAGGTATAACGTTGGCGTGAGCATCTTCACGCAAACg                                                                                                        >  1:8028413/1‑140 (MQ=255)

                                tGCCGGATTCCATTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATCGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACg                                                                                                        <  1:7699994/140‑1 (MQ=255)

                                  ccGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTg                                                                                                      >  2:7041878/1‑140 (MQ=255)

                                          cTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                              <  2:385577/140‑1 (MQ=255)

                                          cTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACt                                                                                              >  1:9577376/1‑140 (MQ=255)

                                            ttGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTg                                                                                            >  1:6071977/1‑140 (MQ=255)

                                            ttGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTg                                                                                            >  2:4652036/1‑140 (MQ=255)

                                               cGGCTGGTCGGCGGGGCTGCCAGGCCCGCAGGCGATCGCGCAGGGACCGGCGTGCGCGGCGACAACCATTCAGTCCCACGGGGCCTGGGCGGTACACCGGTGGCGTGAGCATCTGCACGCCAACGTGCTGTTACTTGCCg                                                                                         <  2:8020374/140‑1 (MQ=255)

                                                    ggTCTGGGGGGATGACATTACCGCAGGCGATAGCGCAGGTAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCTTGAGCTTCTTCACGCCAAGGTGCTGTTACTTGCCGGaaaa                                                                                    <  1:8624095/140‑1 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                    >  1:5941876/1‑140 (MQ=255)

                                                    ggTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGaaaa                                                                                    >  2:4028530/1‑140 (MQ=255)

                                                     gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAAc                                                                                   >  1:7826320/1‑140 (MQ=255)

                                                     gTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACG‑TGGCGTGTGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  2:4109073/140‑1 (MQ=255)

                                                      tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGTGCATCTTCACGCCAACGTGATGTTTCTTGCCGGAAAACg                                                                                  <  2:5851346/140‑1 (MQ=255)

                                                      tCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACg                                                                                  <  2:9835578/140‑1 (MQ=255)

                                                           ggggATGACATTACCGCAGGCGATTGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTTGGTTTAAGGTTGGCGTGTGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCt                                                                             <  1:732372/140‑1 (MQ=255)

                                                               aTGACATTACCGCAGGCGATAGCGCAGGGACCGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                                         >  1:3039740/1‑140 (MQ=255)

                                                               aTGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATaa                                                                         >  2:7609228/1‑140 (MQ=255)

                                                                tGACATTACCGCTGGCGATAGCGCAGGGAACGGGGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACTTTGGCGTGAGAATCTTCACGCCAACTTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                                        <  2:619584/140‑1 (MQ=255)

                                                                tGACATTACCGCAGGCGTTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATTACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAAt                                                                        <  2:5691683/140‑1 (MQ=255)

                                                                tGACATTACCGCAGGCGATCGCGCAGGGAACGGCGTGCGCGGCTAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTTTTACTTGCCGGAAAACGACCCTATAAt                                                                        <  1:232275/140‑1 (MQ=255)

                                                                tGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCAGTTACTTGCCGGAAAACGACCCTATAAt                                                                        >  1:8279998/1‑140 (MQ=255)

                                                                    aTTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCTAAAACCATTCAGTACCACGGTGCCTGTTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGa                                                                    <  1:5858101/140‑1 (MQ=255)

                                                                          gCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGTTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAatt                                                              <  1:4873182/140‑1 (MQ=255)

                                                                            aGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattca                                                            >  1:427047/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcat                                                           >  1:1498784/1‑140 (MQ=255)

                                                                             ggCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGG‑AAACGACCCTATAATCCGAGTAattcatt                                                          <  1:1638968/140‑1 (MQ=255)

                                                                              gCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAattcatt                                                          >  1:9877589/1‑140 (MQ=255)

                                                                                gagcgcgcAGGGCACGGCGGGCGCGGCGAAAACCATGCAGTACCACGGGGCCTGGTAGGTATACCGATGGCGTGACCCACTTCACGCCAACGTGCTGTGACTGGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCt                                                        <  2:11217332/136‑1 (MQ=255)

                                                                                 aTAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCtt                                                       <  1:11420103/140‑1 (MQ=255)

                                                                                      gcAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGTTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTAttt                                                  <  2:10181502/140‑1 (MQ=255)

                                                                                       cAGGGACCGCCGTGCGCGGCGACAACCATGCCGGACCCCGGTGCCGGGGAGGTAAAACGTTGGCGTGCGCATCTTCACGCCAACGTGCTGTTACTGGCCGTAAAACGACCCTCTAATCCGAGTAAATCATTCTTTAttgc                                                 <  2:1183313/140‑3 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCa                                                >  2:6613441/1‑140 (MQ=255)

                                                                                        aGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGAAAGTCATTCTTTATTTCa                                                >  2:2014505/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAgg                                              >  1:1914050/1‑140 (MQ=255)

                                                                                          ggAACGGCGTGCGCGGCGAAAACCATTCAGGACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCTAGTAATTCATTCTTTATTTCAgg                                              <  1:8852395/140‑1 (MQ=255)

                                                                                           gAAGGGCGTGCGCGGCGAAAACCATTCATTACCACGGTGCCTGTTAGGTATAAGGTGGGCGTGAGCTTCTTCACGCCAACTTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAggg                                             <  1:5397623/140‑1 (MQ=255)

                                                                                           gAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCGGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCACCGTGCTGTTACTTGCCGGAAAACGACCCTATCATCCGAGTAATTCAGTCTTGATTTCAggg                                             <  2:7446352/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGa                                         <  1:11156973/140‑1 (MQ=255)

                                                                                               ggCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCGTCACGCCAACGTGCTGTAACTTGCCGGAACACTACCCTATACTCCGAGTAATTCATTCTTTATTTCAGGGTAGa                                         <  2:5181876/140‑1 (MQ=255)

                                                                                                 cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                       >  2:10507734/1‑140 (MQ=255)

                                                                                                 cGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGAtt                                       >  2:8218441/1‑140 (MQ=255)

                                                                                                   tGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTAt                                     >  2:920446/1‑140 (MQ=255)

                                                                                                     cgcgGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAATCGACCCTATAATCCGAGTAATTCATCCTTTATTTCAGGGTCGATTATGt                                   >  1:346852/1‑140 (MQ=255)

                                                                                                     cgcgGAGAAAACCTTTCAGTACCACGGTGCGTGTTAGGAATAACGTTGGCGTGAGCATATTCACGCCAACGTGCTGTTACTTTCCGGAAAACGACCATATAATCCGATTAATTCATTCTTTATTTCAGGGTCGATTATGt                                   <  2:3058522/140‑1 (MQ=255)

                                                                                                      gcggcgAAAACCATTCAGTACCACGGTGCCTGGTTGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTc                                  <  1:3719898/140‑1 (MQ=255)

                                                                                                      gcggcgAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTc                                  >  1:9486072/1‑140 (MQ=255)

                                                                                                            caaaCCATTCAGGACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTAc                            <  1:6877879/139‑1 (MQ=255)

                                                                                                             caaCCATTCAGTACCCCGGTGCCTGGTATGTATAACGTTGGCGTGAGCATCTACACGCCATCGTGCTGTTACTTGCCGGAAAACGACACTATAATCCGCGGAATTCATTCTTTTTTTCAGGGTCGATTATGTCAGCTAct                           <  1:10597657/139‑2 (MQ=255)

                                                                                                             acaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGACAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  2:7275200/138‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTCCCACGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:10121168/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:10360082/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  2:1677961/140‑1 (MQ=255)

                                                                                                             aaaCCATTCAGTACCACGGTGCCTGGTAGGTATAACGTGGGCGTGAGCATCTACACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACg                           <  1:7456864/140‑1 (MQ=255)

                                                                                                                ccATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGAc                        <  1:7113790/140‑1 (MQ=255)

                                                                                                                  aTTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCg                      <  2:286253/140‑1 (MQ=255)

                                                                                                                     cAGTACCCCGGTGCCTGGTAGGTATACCGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTGGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  1:3154493/140‑1 (MQ=255)

                                                                                                                     cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  1:6440230/140‑1 (MQ=255)

                                                                                                                     cAGTACCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTa                   <  1:7609228/140‑1 (MQ=255)

                                                                                                                         aCCACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTActct               >  1:10674323/1‑140 (MQ=255)

                                                                                                                           cACGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAAGTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtca             >  1:8361176/1‑140 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGGTGTTCCTTGCCGGAAATCGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc           <  2:11554539/140‑1 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc           >  1:3829059/1‑140 (MQ=255)

                                                                                                                             cGGTGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCtcatc           >  2:8991583/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc        >  1:3657665/1‑140 (MQ=255)

                                                                                                                                tGCCTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAAc        >  1:2798383/1‑140 (MQ=255)

                                                                                                                                  ccTTGTAGGTATAACGTTGGCTTGAGCATCTTCACGCCAACGTGCTGTTAGTTGCCGTAAAACGACCCTATAATCCGAGTAATTCATTATTTATTTCAGGGTCGATTATGTCATCTACGGACCGCTACTCTCATCAActc      <  1:9317512/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      <  1:3346335/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      >  2:9673947/1‑140 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      <  1:6271115/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCAAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      <  1:2418663/140‑1 (MQ=255)

                                                                                                                                  ccTGGTAGGTAAAACGTTGCCGTGAGCCTCTTCACGCCAACGTGCTGTTACTTGCCGGAACACGACCCTATAATCCGAGTAATTCATTCTGTCTTTCAGGGTCGATTATGTCAGCTCCGGACCGCGACTCACATCAActc      >  2:3260195/1‑140 (MQ=255)

                                                                                                                                  ccTGCTAGGTATAACGTTGGCGTGAGCATCTTCACGCCACCGTGCTGTTACTTGCCGGAAAACGACCCTATCATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActc      <  1:6661218/140‑1 (MQ=255)

                                                                                                                                    tGGTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAActcct    <  2:3778629/140‑1 (MQ=255)

                                                                                                                                      gTAGGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCctct  >  2:10090962/1‑140 (MQ=255)

ATCGACACCATGGGTGCCGGAGATTCGTTCATTGCCGGATTCCTTTGCGGCTGGTCTGCGGGGATGACATTACCGCAGGCGATAGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAAGTATAACGTTGGCGTGAGCATCTTCACGCCAACGTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGACCGCTACTCTCATCAACTCCTCT  >  NZ_CP025268/3504501‑3504774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`