BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	345,338	C→T	L17L (TTG→TTA)	yahN ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	345,338	0	C	T	99.5%	2497.6 / NA	651	L17L (TTG→TTA)	yahN	LysE family transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (399/249); minor base G (2/1); total (401/250)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 651 total aligned reads displayed.

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  1:5400566/140‑1 (MQ=255)

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  1:9187731/140‑1 (MQ=255)

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  2:1274393/140‑1 (MQ=255)

cccAGCGCCACGCCCAGCCCGCTCAGCACCCCTGCGCGTCGACCGGAAGCCAGCCTGGTTTGTACTACCACACAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACCGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                        <  2:10955631/140‑1 (MQ=255)

   aGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCATACGGTCAGGTAAACTGCATGTAAAgg                                                                                                                                     >  1:8860235/1‑140 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                   <  2:3063958/140‑1 (MQ=255)

        cACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCa                                                                                                                                <  2:3277684/140‑1 (MQ=255)

        cACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCa                                                                                                                                <  1:10762648/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              <  1:6724874/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                              >  2:4559075/1‑140 (MQ=255)

               aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAt                                                                                                                         >  1:4746639/1‑140 (MQ=255)

               aGCCCGGTCAGCAACCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAt                                                                                                                         >  1:9985121/1‑140 (MQ=255)

                 cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAttt                                                                                                                       >  1:5092287/1‑140 (MQ=255)

                 cccGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAttt                                                                                                                       <  2:9336949/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                    <  1:7230313/140‑1 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                   >  2:10321304/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                   >  2:4668099/1‑140 (MQ=255)

                      tCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATcc                                                                                                                  >  2:7054165/1‑140 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCCGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGGCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                         >  1:10356344/1‑140 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCCGGCTGGTTTGGACTACCACCAAGAGATTGGCTCCCGGATTAAAAAAAGTCATCACGAACAGTCCTCCGGTCAGGTAAAGGGCATGTAAAGGTTCCATGGTGATGTCAGCCATGAGTAAg                                                                                                         >  2:4886403/1‑140 (MQ=255)

                                       cGACCGGAAGCCAGGCTTTTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTTATCACGAACAGTCCTACGGTCATGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                                 <  1:7331573/140‑1 (MQ=255)

                                          ccGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACGAACTg                                                                                              <  1:2721419/140‑1 (MQ=255)

                                             gAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcat                                                                                           <  1:3117399/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                               >  1:1633492/1‑140 (MQ=255)

                                                            tGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCaaa                                                                            >  1:6956522/1‑140 (MQ=255)

                                                              tactacCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAAAAGTCATACGGTCAGGTAAACGCCATGTAAACTATCCATACTCATTTCATCAAAAAATAAGTGAACTAACTGCATCATATATCTACCaaaaa                                                                          >  1:8031055/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcat                                                                                           >  2:3648045/1‑118 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:5691773/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:10984324/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:4387725/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  2:2383627/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     >  1:2615286/1‑140 (MQ=255)

                                                                    cacaAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcat                                                                                           >  2:8332689/1‑117 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  2:156288/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  2:10894768/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  2:10687713/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                                  >  1:4123895/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAtt                                                                                     >  2:5361149/1‑119 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                                >  1:5855137/1‑140 (MQ=255)

                                                                            gaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgag                                                            <  1:3444408/140‑1 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATc                                                                                                                                  >  2:9740089/1‑69 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:8630774/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:7404556/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:2269701/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  2:2154527/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:11212568/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:1001989/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                           >  1:10875399/1‑140 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                          <  1:4368128/140‑1 (MQ=255)

                                                                               tGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagaga                                                         >  1:755775/1‑140 (MQ=255)

                                                                                     ccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTACAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATCATCGGGTGCGAGAGAGATcac                                                   >  2:5594667/1‑140 (MQ=255)

                                                                                     ccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac                                                   >  1:8672890/1‑140 (MQ=255)

                                                                                     ccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac                                                   >  2:2526530/1‑140 (MQ=255)

                                                                                     ccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac                                                   >  1:8364138/1‑140 (MQ=255)

                                                                                       ggATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                 >  2:6632959/1‑140 (MQ=255)

                                                                                       ggATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                 <  1:7848491/140‑1 (MQ=255)

                                                                                       ggATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                 >  1:3289403/1‑140 (MQ=255)

                                                                                         aTTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAg                                               <  2:4100840/140‑1 (MQ=255)

                                                                                              aaaaaGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGGGTCt                                          <  2:2027259/140‑1 (MQ=255)

                                                                                                aaaGTAATCCCGACCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGGGATTTCATCCATAAATAAGTGTACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTa                                        <  1:9691437/140‑1 (MQ=255)

                                                                                                  aGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTagt                                      >  2:8016071/1‑138 (MQ=255)

                                                                                                  aGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAtt                                      >  1:3922434/1‑140 (MQ=255)

                                                                                                  aGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTAtt                                      >  2:4156378/1‑140 (MQ=255)

                                                                                                     aaTCACGAACAGTCCTACGGTCAGGTAAAAGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTACCTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTcc                                   <  2:11270234/140‑1 (MQ=255)

                                                                                                      aTCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCg                                  >  1:2156269/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAATGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:7486015/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                  <  1:1404816/113‑1 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:8713750/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:2097441/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:9779285/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  2:9009635/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:10635727/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                       >  1:2900241/1‑140 (MQ=255)

                                                                                                                  ccgaCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  2:11132375/137‑1 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                      <  2:11489353/140‑1 (MQ=255)

                                                                                                                   cTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGttt                     <  1:4558976/140‑1 (MQ=255)

                                                                                                                    tACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTa                    <  2:7851399/140‑1 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  1:8991308/1‑140 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  2:1361685/1‑140 (MQ=255)

                                                                                                                     aCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTAt                   >  1:11521365/1‑140 (MQ=255)

                                                                                                                      cGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATg                  >  2:3433503/1‑140 (MQ=255)

                                                                                                                       ggTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGc                 <  1:5273851/140‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGa                                                                                                      <  1:8230162/51‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:8538080/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:6008773/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:7593213/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:612930/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  2:230635/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:3638893/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg             >  1:2422107/1‑140 (MQ=255)

                                                                                                                            ggTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctga            <  1:1989144/140‑1 (MQ=255)

                                                                                                                                aaCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCATATTTCCGGTTACTGTCGGGTATGCCctgactga        >  1:6465327/1‑140 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                     <  2:618769/78‑1 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa       <  2:10573564/140‑1 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa       <  1:7730662/140‑1 (MQ=255)

                                                                                                                                  cGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAAc      >  1:10246622/1‑140 (MQ=255)

                                                                                                                                  cGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAAc      <  2:2705177/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt     >  2:816371/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATACATAGTGATTTCATCCATAAATAAGTCAACTAACTGCATCATATTTCTAACAAAAATAATCGGGTGCGAGTGAGATCACAAATTGTCTTATTTCCGGTTACTTGCGTTTATGCCCTGACTGAACt     <  2:10161160/140‑1 (MQ=255)

                                                                                                                                     cATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTaa   >  1:11325742/1‑140 (MQ=255)

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAAT  >  NZ_CP025268/345201‑345474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`