BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,844,378	T→C	445 (TAA→TAG)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,844,378	0	T	C	99.9%	2379.7 / NA	682	445 (TAA→TAG)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (286/395); minor base A (1/0); total (287/395)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 689 total aligned reads displayed.

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCG  >  NZ_CP025268/3844242‑3844514

aaTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTaa                                                                                                                                       <  2:2657182/140‑1 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  2:5492725/140‑1 (MQ=255)

             gCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCCGCGTGGCGAGCCTTATCCAGATCATGAGTTATCCTGCGTAATCAGAACTAAAGATATCTGGAAACCCACGCCCGTTAATAATCCACCAGaataa                                                                                                                          >  2:2255677/1‑140 (MQ=255)

                ccGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCt                                                                                                                       <  2:4992500/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACCCGACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  1:975477/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  2:6257467/140‑1 (MQ=255)

                    aTGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAg                                                                                                                   >  1:5981204/1‑140 (MQ=255)

                     tGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGt                                                                                                                  <  1:4597179/140‑1 (MQ=255)

                         aGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaa                                                                                                              >  1:8568850/1‑140 (MQ=255)

                          gCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                             <  1:11508992/140‑1 (MQ=255)

                          gCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                             <  1:1704312/140‑1 (MQ=255)

                           cACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAAc                                                                                                            >  2:1550625/1‑140 (MQ=255)

                           cACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAAc                                                                                                            <  2:1106350/140‑1 (MQ=255)

                            aCCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACa                                                                                                           <  2:4033386/140‑1 (MQ=255)

                               cGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACagag                                                                                                        <  2:10272659/140‑1 (MQ=255)

                                gAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGc                                                                                                       >  2:10018667/1‑140 (MQ=255)

                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                      <  1:11169936/140‑1 (MQ=255)

                                   aaccaaccACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCCGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGc                                                                                                    >  2:10438140/1‑140 (MQ=255)

                                      cacccccaAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAAACAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                                 <  2:10573566/134‑1 (MQ=255)

                                      caaccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                                 <  1:9526435/140‑1 (MQ=255)

                                      caaccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                                 <  2:7972152/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  2:4626485/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  1:10689570/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               >  1:3817439/1‑140 (MQ=255)

                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                              <  1:10221999/140‑1 (MQ=255)

                                            caAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTACTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCACACAGAGCTGCGaccaccac                                                                                           >  2:4787092/1‑140 (MQ=255)

                                              aaCACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                         <  2:1392098/140‑1 (MQ=255)

                                                 acTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacac                                                                                      >  2:900662/1‑140 (MQ=255)

                                                  cgcccGCCAGCGTGGCGAGCCTTATCCAGCTCAGGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  2:9790439/137‑1 (MQ=255)

                                                  ccaCCGCCAGCGTGGCGCGCATTATCCAGCTCAGGAGTTCGCCTGCGTAATCAGCACTCATGATATCTCGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  2:7042116/138‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  1:1500999/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     >  1:9177591/1‑140 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  1:3390548/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  1:6934196/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTACCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  2:3478585/140‑1 (MQ=255)

                                                  cTACAGCCAGCGTCTCGTGCATTATCCAGATCATGAGTTCTCCTGGTTAATCATAAAACATGATATGTGTAATCCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  2:2493175/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                    <  1:724941/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                    <  1:614502/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                    >  1:10373976/1‑140 (MQ=255)

                                                       gCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGt                                                                                >  1:5878844/1‑140 (MQ=255)

                                                       gCCAGCGTGGCGAACATTAACCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATCTCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCACACAGTGCTGAGACCACCACCACACACAGGt                                                                                >  2:7371930/1‑140 (MQ=255)

                                                           gCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATTATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCTAACAGAGCTGCGACCACCACCACACACAGGTTCAg                                                                            >  2:7595420/1‑140 (MQ=255)

                                                             gTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGAt                                                                          <  1:10018667/140‑1 (MQ=255)

                                                                 cGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgcg                                                                      >  1:4726059/1‑140 (MQ=255)

                                                                 cGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgcg                                                                      >  1:8137743/1‑140 (MQ=255)

                                                                    gCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCa                                                                   <  1:7584385/140‑1 (MQ=255)

                                                                        tctCCAGATCATGCGTTCTCCGGCGTAATCAGAACTCATGAGATCGGGAAACCCTCGCCAGTTACTCCTCCACCAGAATAATCTTCAGTGCAATCAGAGCTGCGACCACCAACACACACAGGTTCAGATCGCGCCAgcgc                                                               <  2:10802524/138‑1 (MQ=255)

                                                                        tagccAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGACACCCTCGCCAGTTACTCATCCACCAGCATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                               <  2:431711/137‑1 (MQ=255)

                                                                        tATCCAGATCATGAGTTCTCCTGCGTGATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCCCCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                               <  1:8289534/140‑1 (MQ=255)

                                                                         aTCCCGACCCTGAGTTCTCCGGCGTAATCAGAACTCATGATATCTGGCAACCCTCGCCAGTTACTACTCCCCCCCAATAATCTGCAGTGCAAACAGAGCTGCGACCACCACCACACCCAGGTTCAGATCGCGCCAGCGcc                                                              <  2:11569203/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  1:7465703/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  2:8098712/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  2:949249/140‑1 (MQ=255)

                                                                           ccAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCg                                                            <  1:7013063/140‑1 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                        >  1:9046569/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                        >  1:1375404/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                        >  1:4036661/1‑140 (MQ=255)

                                                                                tCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                       <  1:5136575/140‑1 (MQ=255)

                                                                                tCAGGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                       <  2:9209229/140‑1 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:5584804/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  1:3630584/1‑140 (MQ=255)

                                                                                      gTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACttt                                                 <  1:7360815/140‑1 (MQ=255)

                                                                                       ttCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTc                                                >  1:889412/1‑140 (MQ=255)

                                                                                        tctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                               <  1:7668531/140‑1 (MQ=255)

                                                                                        tctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAAGCCCCCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                               <  2:604794/140‑1 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  2:1925544/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  2:2914172/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGGGCATACTTTCat                                              >  2:5235022/1‑140 (MQ=255)

                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  1:6364194/54‑1 (MQ=255)

                                                                                              gCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGTGCTGCGACCACCACCACACACAGGTTCAGCTCGCGCCAGCGCCCGGTGCATACTTTCatgtggc                                         >  2:8436503/1‑136 (MQ=255)

                                                                                              gCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGc                                         <  1:5416788/140‑1 (MQ=255)

                                                                                               cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCa                                        >  2:3831233/1‑140 (MQ=255)

                                                                                               cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCa                                        >  1:9436780/1‑140 (MQ=255)

                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGt                                      >  2:8708450/1‑140 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACCCAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                     <  2:3684752/140‑1 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                     <  1:2111009/140‑1 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGGTACTAATCCACCAGACTAATCTTCAGTGCAAACAGAGCTGCGACCACCACCCCACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                     <  2:6461098/140‑1 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  1:11297916/1‑140 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  2:2092558/1‑140 (MQ=255)

                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGc                          <  2:3082356/140‑1 (MQ=255)

                                                                                                              tGATATCTGGAAACCCTCTCCAGTAACTAATCCACAAGAATAATCTACAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGAGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                         <  2:11348236/140‑1 (MQ=255)

                                                                                                              tGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                         <  1:5775907/140‑1 (MQ=255)

                                                                                                              tGATATCTGGAAACCCACGCCAGTTACTCATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACGTTCATGATGCAGTACGACATAAAGcc                         <  2:6700422/140‑1 (MQ=255)

                                                                                                                  atCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCTACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAATCCGAGt                     >  1:6623985/1‑140 (MQ=255)

                                                                                                                  atCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGt                     >  2:7155104/1‑140 (MQ=255)

                                                                                                                    cTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGc                   <  1:3187720/140‑1 (MQ=255)

                                                                                                                     tGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa                  <  1:2914172/140‑1 (MQ=255)

                                                                                                                      ggAACCCCCCGCCAGTTACGCAGCCACCAGAATAATCTTCAGTGCACACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa                 <  2:578758/140‑1 (MQ=255)

                                                                                                                             cTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTc          >  1:4502584/1‑140 (MQ=255)

                                                                                                                              tCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCg         >  1:4334220/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCGGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  1:10881922/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  2:2790637/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  1:5514422/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  1:2650332/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  1:7854406/1‑140 (MQ=255)

                                                                                                                                 ccAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTg      >  1:1806329/1‑140 (MQ=255)

                                                                                                                                 ccAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTg      >  2:5954029/1‑140 (MQ=255)

                                                                                                                                   aGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATACAGCCGAGTGCAATCCCTTCGGTGAt    <  1:2344028/140‑1 (MQ=255)

                                                                                                                                   aGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGAt    <  2:1788431/140‑1 (MQ=255)

                                                                                                                                     ttactaATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCg  >  1:1182342/1‑140 (MQ=255)

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCG  >  NZ_CP025268/3844242‑3844514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`