Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,915,245 A→G C115R (TGT→CGT)  glmS ← glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,915,2450AG99.7% 3623.2 / NA 1040C115R (TGT→CGT) glmSglutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (575/461);  minor base T (0/3);  total (576/464)
Fisher's exact test for biased strand distribution p-value = 8.87e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1048 total aligned reads displayed.

CACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATA  >  NZ_CP025268/3915108‑3915381
                                                                                                                                         |                                                                                                                                        
    gTACCGTACTCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTTTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCTGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    <  2:9467325/140‑1 (MQ=255)
    gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    <  2:5328535/140‑1 (MQ=255)
    gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    >  1:4097389/1‑140 (MQ=255)
    gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                    >  1:7477596/1‑140 (MQ=255)
     tACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtttt                                                                                                                                   >  1:6799203/1‑140 (MQ=255)
     tACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtttt                                                                                                                                   >  1:3377582/1‑140 (MQ=255)
      aCCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                  <  1:8649043/140‑1 (MQ=255)
       ccGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCATGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTACAGACACGAAGGTAAAGCCACGCGCTTATAg                                                                                                                                 >  2:3992997/1‑140 (MQ=255)
        cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                                <  1:9124354/140‑1 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCTGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTGAGAAACGTAGGTATAGCCTCGCGCTTTTAGctc                                                                                                                              >  2:1617051/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGCTGGGCAATCACGTCGGTGGCGGTGTCAGAAACGAAGGTATAGCCACGCGCTGTTAGctc                                                                                                                              >  2:11472025/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:3576847/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:5552293/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:4150314/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:4261070/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:1065747/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:6252093/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:7210116/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:235606/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  1:2143741/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:1178327/1‑140 (MQ=255)
          tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTACACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                              >  2:1949278/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:10919287/1‑140 (MQ=255)
             gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                           >  1:8296323/1‑140 (MQ=255)
               accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                         >  2:10115683/1‑140 (MQ=255)
               accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                         >  1:10392984/1‑140 (MQ=255)
                 cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                       >  2:5414624/1‑140 (MQ=255)
                 cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTAGAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                       >  1:11229317/1‑140 (MQ=255)
                  acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                      <  1:1892992/140‑1 (MQ=255)
                  acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCGTTTAGCTCTTCACGCa                                                                                                                      >  2:11216766/1‑140 (MQ=255)
                   cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                     <  2:2356533/140‑1 (MQ=255)
                   cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                     <  1:5009470/140‑1 (MQ=255)
                   cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                     >  1:10525542/1‑140 (MQ=255)
                    gCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGc                                                                                                                    >  2:8186476/1‑140 (MQ=255)
                        cTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGtt                                                                                                                >  2:6756110/1‑140 (MQ=255)
                         tGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTCTAGCCACGCGCGTTTAGCTCTTCACGCAGCGGTTc                                                                                                               >  1:6499197/1‑140 (MQ=255)
                                  gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                      >  1:6006508/1‑140 (MQ=255)
                                  gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGGTTTc                                                                                                      >  2:7802942/1‑140 (MQ=255)
                                     cGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCTTGGTTTTCgat                                                                                                   >  1:1170032/1‑140 (MQ=255)
                                       cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                 <  2:8417852/140‑1 (MQ=255)
                                        aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGGTTCAGAAACGAAGGTTGTGCCACGCGGGTTTAGCGCTTCTCGCAGCGGTTCTTGGTTTGCgatgag                                                                                                >  2:11007249/1‑139 (MQ=255)
                                         gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                               <  1:9357979/140‑1 (MQ=255)
                                          aaCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCCGAAACGAAGGTATAGCCACGCGCGTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGc                                                                                              >  2:8068640/1‑140 (MQ=255)
                                          aaCGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGc                                                                                              >  2:7157142/1‑140 (MQ=255)
                                            cGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCg                                                                                            <  2:10775468/140‑1 (MQ=255)
                                                 tCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGGTCCCCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTAGAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATg                                                                                       <  2:514089/140‑1 (MQ=255)
                                                  cACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGc                                                                                      >  2:10575926/1‑140 (MQ=255)
                                                   aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                     <  2:3659885/140‑1 (MQ=255)
                                                    cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                    <  1:8781394/140‑1 (MQ=255)
                                                    cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                    <  1:294299/140‑1 (MQ=255)
                                                    cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                    <  2:10240009/140‑1 (MQ=255)
                                                        gagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacc                                                                                <  1:10730895/140‑1 (MQ=255)
                                                           tCCCCCCTTGATTCAGCCCCCAGGTCACCCGATCGGCACTCACTGCGGTGTCGGTTACAGAAACGAAGGTAGAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGATTTCGAAGATGCCGTTATGCACCACcaca                                                                             <  2:10435682/140‑1 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTCTAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                           >  2:2440155/1‑140 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                           <  1:10179852/140‑1 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                           <  1:7826734/140‑1 (MQ=255)
                                                             ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                           <  1:7594306/140‑1 (MQ=255)
                                                              cGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAtg                                                                          <  2:6903510/140‑1 (MQ=255)
                                                               gCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCCCGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTGTTCGATGATGCCGTTATGCACCACcacacagt                                                                         >  2:8860456/1‑136 (MQ=255)
                                                                ccTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAtgtg                                                                        >  2:4652207/1‑140 (MQ=255)
                                                                     tttCAGCTCCCAGTTCACCAGATGGGCGATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                   >  1:3939628/1‑140 (MQ=255)
                                                                     tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                   >  2:4423149/1‑140 (MQ=255)
                                                                     tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                   >  1:9827977/1‑140 (MQ=255)
                                                                     tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTGTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGGTATGCACCACCACCCTGTGTGCAg                                                                   >  2:9010919/1‑140 (MQ=255)
                                                                           cTCCCAGTTCATCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACAt                                                             >  2:3163186/1‑140 (MQ=255)
                                                                           cTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACAt                                                             >  2:2822389/1‑140 (MQ=255)
                                                                               cAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCgg                                                         >  2:861408/1‑140 (MQ=255)
                                                                               cAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCgg                                                         >  2:9205337/1‑140 (MQ=255)
                                                                                   tCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACCTGCGGATgc                                                     >  1:7981185/1‑140 (MQ=255)
                                                                                   tCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATgc                                                     <  2:7681737/140‑1 (MQ=255)
                                                                                   tCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATgc                                                     <  1:142223/140‑1 (MQ=255)
                                                                                        aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACACTGTGTTCAGAAACATGCGGATGCGCAtt                                                >  2:7413635/1‑140 (MQ=255)
                                                                                            gggCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACt                                            <  2:6431208/140‑1 (MQ=255)
                                                                                            gggCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACt                                            >  1:2717205/1‑140 (MQ=255)
                                                                                            gggCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACt                                            >  1:8484526/1‑140 (MQ=255)
                                                                                                 aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                       <  1:4750216/140‑1 (MQ=255)
                                                                                                   cACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAg                                     >  1:9607621/1‑140 (MQ=255)
                                                                                                    aCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAgg                                    >  1:11014541/1‑140 (MQ=255)
                                                                                                     cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACACTGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                   <  2:3396319/140‑1 (MQ=255)
                                                                                                     cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                   <  2:2589826/140‑1 (MQ=255)
                                                                                                            gtCGGTTTCAGAAACGAAGGTATATCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATTCCGTTAAGCACCACCACCATGTGATCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGt                            >  2:3053076/1‑140 (MQ=255)
                                                                                                            gtCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGt                            >  1:11598099/1‑140 (MQ=255)
                                                                                                            gtCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGt                            >  1:5701020/1‑140 (MQ=255)
                                                                                                              cGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTgg                          <  2:3806414/140‑1 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        <  1:1606262/140‑1 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        <  1:5210596/140‑1 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        >  1:3157120/1‑140 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        <  1:5388752/140‑1 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        <  1:6999276/140‑1 (MQ=255)
                                                                                                                gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCACTTCACGCAGCGTTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCACAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                        <  2:5017805/140‑1 (MQ=255)
                                                                                                                  ttCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCg                      <  1:7210116/140‑1 (MQ=255)
                                                                                                                     aGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGccc                   >  2:5922216/1‑140 (MQ=255)
                                                                                                                         aCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCg               >  1:6289861/1‑140 (MQ=255)
                                                                                                                            aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt            <  2:2195991/140‑1 (MQ=255)
                                                                                                                             aGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgtg           <  1:3811723/140‑1 (MQ=255)
                                                                                                                               gTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg         <  1:458927/140‑1 (MQ=255)
                                                                                                                               gTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg         <  2:1449901/140‑1 (MQ=255)
                                                                                                                                 ataGCCACGCGCATTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAg       <  2:5768139/140‑1 (MQ=255)
                                                                                                                                   aGCCACGCGCTTTTAGCTATTCACGCAGCGGATCATGGTTTTCGATGATGCAGTTATGCACCACCACAAATGGTTCAGAAACATGCTGATTCGCATTCACTTCTTAATGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCa     >  2:9745087/1‑140 (MQ=255)
                                                                                                                                     ccACGCGCATTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAt   <  1:10051795/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAATA  >  NZ_CP025268/3915108‑3915381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑