BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,119,045	A→G	C34R (TGC→CGC)	glpK ←	glycerol kinase GlpK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,119,045	0	A	G	99.6%	2850.5 / ‑1.6	819	C34R (TGC→CGC)	glpK	glycerol kinase GlpK
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (325/491); minor base T (2/1); total (327/492)
Fisher's exact test for biased strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

Only 100 of 828 total aligned reads displayed.

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTT  >  NZ_CP025268/4118909‑4119181

ccGATAGCTTCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                                                                                                                                       >  2:4730736/1‑140 (MQ=255)

ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTGTTGGGTAGATTTGCTCAAATTCGCGCTg                                                                                                                                       >  1:10002896/1‑140 (MQ=255)

ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCGGGTTTTGGGTAGATTTGCTCAAATTCGCGCTg                                                                                                                                       >  2:4208683/1‑140 (MQ=255)

  gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCCCAACCTGGTTTTGGGTCGATTTGCTCAAATTCGCGCTg                                                                                                                                       >  1:8508709/1‑140 (MQ=255)

   atcgCTGCAAGTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTGTGGGTGGCCCAGATTTCCATTGGGTCGGGTTCTA‑‑CCCAACCTGGTGTTGGGTAGATTTGCTCAACTGCGCGCTGCGa                                                                                                                                    >  2:6102897/4‑140 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGNGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTCGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    >  1:6160190/1‑140 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    <  2:3281406/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    <  1:6401898/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    <  1:4537274/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAAACTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    >  2:4039982/1‑140 (MQ=255)

    tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                   <  1:7769342/140‑1 (MQ=255)

    tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                   >  1:1296731/1‑140 (MQ=255)

    tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGac                                                                                                                                   <  1:7477277/140‑1 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGaca                                                                                                                                  <  1:5397674/140‑1 (MQ=255)

        tGCAATGTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTGGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGc                                                                                                                               <  1:7662252/140‑1 (MQ=255)

          cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                             <  1:9649168/140‑1 (MQ=255)

          cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTa                                                                                                                             <  1:9996856/140‑1 (MQ=255)

           aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTaa                                                                                                                            <  1:3474462/140‑1 (MQ=255)

            aTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                                                                                           <  2:4413937/140‑1 (MQ=255)

            aTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAAt                                                                                                                           <  1:5016100/140‑1 (MQ=255)

                 aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCg                                                                                                                                     <  2:1557265/125‑1 (MQ=255)

                 aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGatat                                                                                                                      >  2:2482821/1‑140 (MQ=255)

                 aTCGGAACGGATATCGGCTTTCGCCCGCACTTCTACCAGCGTGGAGCTTGGGGTGGCCCAGATTTCCAGTGGGGCGTGTTCTA‑‑ACCAACCTGGTTTTGGGTAGAGTTGCTCAAATTCGCGCTGCGACACGCTACTGagat                                                                                                                      >  2:1152003/1‑140 (MQ=255)

                    ggAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTgg                                                                                                                   <  1:5099574/140‑1 (MQ=255)

                     gAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGc                                                                                                                  <  1:2451352/140‑1 (MQ=255)

                      aaCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCa                                                                                                                 <  1:923785/140‑1 (MQ=255)

                        cTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATc                                                                                                               <  2:8980707/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                           <  1:4208856/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                           <  1:8069188/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                           <  1:6777432/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATGGGGTCGTGTTCGA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                           <  2:8437392/140‑1 (MQ=255)

                            tatCGGCGTTCGCCAGCACTTCTACCCGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                           <  2:7738380/140‑1 (MQ=255)

                            gagcGGCTCTCGCCAGCACTTCTCCCCGCGTGGAGCTTTGGGTGGCCCAGAATTCCATTGGGTCGGGTTCTA‑‑CCCAACCTGGTTTAGGGTAGATTTGCTCCAATTCGCGCTGCGACACGCAACTGCTATTCGCATCGTGa                                                                                                           <  2:107510/137‑1 (MQ=255)

                                ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCa                                                                                                       >  1:7393271/1‑140 (MQ=255)

                                ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCa                                                                                                       >  2:2584378/1‑140 (MQ=255)

                                ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCa                                                                                                       >  1:3778608/1‑140 (MQ=255)

                                ggCTTTCGACAGGTCTTCTACCAGCGTGGAGCGTTGGGTGGCCCAGATTTCCTTTGGGTCGTGTTCTA‑‑CCCAACCTGGTTGTGGGTTGATTTGGTCATATTCGCGCTGCGACACGCTAATGAGATTGGCGGCGTGATcgg                                                                                                       >  2:2005168/1‑138 (MQ=255)

                                      cGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGa                                                                                                 <  1:11521207/140‑1 (MQ=255)

                                           gCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                            <  2:7318442/140‑1 (MQ=255)

                                           gCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                            <  2:9697345/140‑1 (MQ=255)

                                           gCACTTCTACCAGCGTGGAGCATTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                            <  2:7148017/140‑1 (MQ=255)

                                            cACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGa                                                                                                                                    <  2:7539106/99‑1 (MQ=255)

                                            cACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgc                                                                                           <  1:1675784/140‑1 (MQ=255)

                                            cACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTGTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATAGTGGCATCGTGATCCATTACGACcgcgc                                                                                           >  1:3605503/1‑140 (MQ=255)

                                             aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                          <  1:8783687/140‑1 (MQ=255)

                                                tCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGa                                                                                       <  2:5472589/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTGTTGGTGGCCCAGATTTCCATTGCGTCGTGTTCTA‑‑CCCAACCTGTTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACTCTAAGGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                     <  2:11172031/140‑1 (MQ=255)

                                                      aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                                 <  1:1186953/140‑1 (MQ=255)

                                                       gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                <  2:597053/140‑1 (MQ=255)

                                                       gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                <  1:4371932/140‑1 (MQ=255)

                                                       gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                                >  1:5700280/1‑140 (MQ=255)

                                                        cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                               <  1:8631806/140‑1 (MQ=255)

                                                        cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                               <  2:5609505/140‑1 (MQ=255)

                                                        cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                               <  2:9406265/140‑1 (MQ=255)

                                                        cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                               >  1:5282052/1‑140 (MQ=255)

                                                             aGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                          <  2:3482417/140‑1 (MQ=255)

                                                             aGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAAGTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                          <  2:10297618/140‑1 (MQ=255)

                                                               cTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTg                                                                        >  2:2184046/1‑140 (MQ=255)

                                                                  tGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTc                                                                     >  1:11117239/1‑140 (MQ=255)

                                                                  tGGGTGGCCCAGATGTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTGTGGGTCGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGGGGTGCCCTGGTc                                                                     >  2:6202534/1‑140 (MQ=255)

                                                                       ggCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                                <  1:11349034/140‑1 (MQ=255)

                                                                        gCCCAGATTTCCATGGGGTCGTGTTCGA‑‑CCCAACCTGGTTTGGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcgc                                                               <  2:6656897/140‑1 (MQ=255)

                                                                         cccAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                              >  1:2741984/1‑140 (MQ=255)

                                                                           cAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGTGCGCAAc                                                            >  2:5077375/1‑140 (MQ=255)

                                                                                   cATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt                                                    >  1:9127819/1‑140 (MQ=255)

                                                                                        ggTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATAGTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttttttt                                               >  2:10829/1‑140 (MQ=255)

                                                                                         gTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttttttt                                              <  2:8769787/140‑1 (MQ=255)

                                                                                          tCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTc                                             >  1:4663635/1‑140 (MQ=255)

                                                                                            gtgtTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                           <  1:10448442/140‑1 (MQ=255)

                                                                                            gtgtTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                                           <  1:189011/140‑1 (MQ=255)

                                                                                            gtgtTCTA‑‑CCCAACCTGGTTTTGGGTAGATGTGCTCAAATTCGCGCTGCGACACGCTAATGAGAGTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGAGATATTTTTTTTCAg                                           <  2:6414718/140‑1 (MQ=255)

                                                                                              gtTCTA‑‑CCCAACATGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                                         <  2:6922447/140‑1 (MQ=255)

                                                                                                 cTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                      <  1:2977454/140‑1 (MQ=255)

                                                                                                        cAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                 <  2:1280693/140‑1 (MQ=255)

                                                                                                        cAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                 <  2:5809347/140‑1 (MQ=255)

                                                                                                        cAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                                 <  1:1562054/140‑1 (MQ=255)

                                                                                                          cccTGGTTTTGGGTAGATTTGCTCAACTTCGCGCTGCGACACGCTAATGTTATTGGCTTCGTGATCCATTACGACCGCGCGGGAGCGGGTGGTGCCCTGGTCGAGCGCAACGCTATATTTTTTTTCAGTCATGTTTAAtt                               <  2:9113659/139‑1 (MQ=255)

                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                              >  2:8196920/1‑140 (MQ=255)

                                                                                                           ccTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                              >  1:2209960/1‑140 (MQ=255)

                                                                                                             tGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTc                            <  2:11086299/140‑1 (MQ=255)

                                                                                                               gTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc                          <  2:8012689/140‑1 (MQ=255)

                                                                                                                 tttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                        <  2:11407604/140‑1 (MQ=255)

                                                                                                                 tttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                        <  2:493400/140‑1 (MQ=255)

                                                                                                                 tttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                        <  1:9384463/140‑1 (MQ=255)

                                                                                                                 tttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                        <  1:6625033/140‑1 (MQ=255)

                                                                                                                 tttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTAAGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAAAGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                        <  2:4279109/140‑1 (MQ=255)

                                                                                                                  ttGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATCTATTTTTTTTCAGTCATGTTTAATTGTCCCGTa                       >  2:2970566/1‑140 (MQ=255)

                                                                                                                   tGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                      <  2:10542068/140‑1 (MQ=255)

                                                                                                                   tGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                      <  2:1720903/140‑1 (MQ=255)

                                                                                                                      gTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCa                   <  1:8286086/140‑1 (MQ=255)

                                                                                                                         gATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCatat                >  1:7965559/1‑140 (MQ=255)

                                                                                                                          aTTTGCTCAAATTCGCGCTGCGACACTCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt               <  1:7014381/140‑1 (MQ=255)

                                                                                                                          aTTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt               <  1:5927723/140‑1 (MQ=255)

                                                                                                                                tCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCg         <  2:6849505/140‑1 (MQ=255)

                                                                                                                                   aaTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAg      <  1:1412083/140‑1 (MQ=255)

                                                                                                                                    aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc     >  1:10873503/1‑140 (MQ=255)

                                                                                                                                    aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc     >  1:3364420/1‑140 (MQ=255)

                                                                                                                                    aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc     >  1:9904316/1‑140 (MQ=255)

                                                                                                                                    aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCCGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc     >  1:3101898/1‑140 (MQ=255)

                                                                                                                                       cgcgCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttt  <  2:3833579/140‑1 (MQ=255)

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTA‑‑CCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTT  >  NZ_CP025268/4118909‑4119181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`