Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,529,477 G→A P140L (CCG→CTG)  sgcA ← SgcA family PTS sugar transporter subunit IIA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,529,4770GA99.9% 2769.6 / NA 723P140L (CCG→CTG) sgcASgcA family PTS sugar transporter subunit IIA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (417/305);  minor base T (0/1);  total (417/306)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 723 total aligned reads displayed.

CTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCGGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAAGCAGCCA  >  NZ_CP025268/4529340‑4529613
                                                                                                                                         |                                                                                                                                        
ctgctgTCCTCAATATCCAGATGCAGGCTCCCAAACTCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgc                                                                                                                                        <  2:3233511/140‑1 (MQ=255)
ctgctgACCTCAATATCCAGATGCAGGCTCCCAACATCCAGATGGTCCAATGCCGGCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAgc                                                                                                                                        <  1:7560487/140‑1 (MQ=255)
 tgctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATAATAAGCGCCTCCGTTAACCTGATACCAgcg                                                                                                                                       <  1:4556280/140‑1 (MQ=255)
   ctgTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCa                                                                                                                                     >  2:3406612/1‑140 (MQ=255)
     gTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCaaa                                                                                                                                   >  2:8788910/1‑140 (MQ=255)
      tCCTCAATCTCCAGATGCAGGCTCCCCAAATCCAGATTGTCCAAGGCCGTCAGCTCCCGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAAc                                                                                                                                  <  1:9699331/140‑1 (MQ=255)
      tCCTCAATATCCAGAGGCAGGCTCCCAAACTCCAGATTGTCCCATGCCGTCAGCTCACGACCATCGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAAc                                                                                                                                  <  2:2494126/140‑1 (MQ=255)
        cTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAg                                                                                                                                >  2:235619/1‑140 (MQ=255)
        cTCAATAGCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGTGAGACGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAg                                                                                                                                <  1:4239184/140‑1 (MQ=255)
          cAATATCCAGATGCTGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATGTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGGTAACCTGATACCAGCGCAAACAGTg                                                                                                                              >  2:9710975/1‑140 (MQ=255)
             tatCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCAt                                                                                                                           >  2:3835027/1‑140 (MQ=255)
                ccAGATGCAGGCTCCCAAACTCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        <  1:10902874/140‑1 (MQ=255)
                ccAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        <  1:4895206/140‑1 (MQ=255)
                ccAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGt                                                                                                                        <  2:9260227/140‑1 (MQ=255)
                   gATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCtg                                                                                                                     >  1:9344385/1‑140 (MQ=255)
                    aTGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCtgt                                                                                                                    >  2:11014306/1‑140 (MQ=255)
                      gCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGc                                                                                                                  <  1:2366949/140‑1 (MQ=255)
                       cAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCt                                                                                                                 >  1:3608899/1‑140 (MQ=255)
                       cAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCt                                                                                                                 >  1:7358999/1‑140 (MQ=255)
                        aGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTg                                                                                                                <  1:4020309/140‑1 (MQ=255)
                        aGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTg                                                                                                                <  2:4967219/140‑1 (MQ=255)
                           cTCCCACAATCCACATGCGCCAATGCCCTCAGCTCACGACCATCGTGCAGAGGATTTGCGCGGGCGAGAGCAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCa                                                                                                             <  2:10858956/140‑1 (MQ=255)
                           cTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCa                                                                                                             <  2:7714763/140‑1 (MQ=255)
                           cTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCa                                                                                                             <  1:8326791/140‑1 (MQ=255)
                              ccAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCAttt                                                                                                          >  2:8574951/1‑140 (MQ=255)
                              ccAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCACCGTCTGTGCTGGc                                                                                                              <  1:9970684/136‑1 (MQ=255)
                                aaaaTCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTcc                                                                                                        <  1:7447808/140‑1 (MQ=255)
                                    tCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACt                                                                                                    >  2:1815758/1‑140 (MQ=255)
                                     ccAGATTGTCCAATGCCGTCAGCTCACGACCAGAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTg                                                                                                   <  1:1065806/140‑1 (MQ=255)
                                        gATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCg                                                                                                >  2:8340712/1‑140 (MQ=255)
                                        gATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCg                                                                                                >  2:9876650/1‑140 (MQ=255)
                                          ttGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGtt                                                                                              <  2:529040/140‑1 (MQ=255)
                                              ccAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGc                                                                                          >  2:3744815/1‑140 (MQ=255)
                                                aaTGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGt                                                                                        <  2:1572354/140‑1 (MQ=255)
                                                     cGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTgg                                                                                   >  1:10124344/1‑140 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 >  2:2672169/1‑140 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 <  1:8512682/140‑1 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 >  2:7974038/1‑140 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 >  1:9186346/1‑140 (MQ=255)
                                                       tCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCg                                                                                 >  1:11624415/1‑140 (MQ=255)
                                                                   cATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACt                                                                     <  2:10416857/140‑1 (MQ=255)
                                                                     tAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATCAGCGCCTCCGTTCACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTgg                                                                   >  2:10663990/1‑140 (MQ=255)
                                                                     tAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGGTGTGGCGAATCAATAAACTgg                                                                   >  1:1722468/1‑140 (MQ=255)
                                                                        tGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTg                                                                <  2:2208368/140‑1 (MQ=255)
                                                                          cAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGAt                                                              <  1:1909424/140‑1 (MQ=255)
                                                                             agGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATgcg                                                           >  1:2486857/1‑140 (MQ=255)
                                                                             agGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATgcg                                                           >  2:7528325/1‑140 (MQ=255)
                                                                             agGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATgcg                                                           >  1:5103671/1‑140 (MQ=255)
                                                                               gATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCt                                                         >  1:3408829/1‑140 (MQ=255)
                                                                                aTTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCAtt                                                                                                           >  1:1000527/1‑89 (MQ=255)
                                                                                 tttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGa                                                       <  1:5987890/140‑1 (MQ=255)
                                                                                 tttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGAGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGa                                                       <  2:2953328/140‑1 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGaa                                                      >  1:2192038/1‑140 (MQ=255)
                                                                                  ttGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGGTAAGCGTTGTGGCGTATCAATAAACTGGCTGAGGCGCTGag                                                      >  2:8169218/1‑139 (MQ=255)
                                                                                      gcTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGt                                                  <  1:7999885/140‑1 (MQ=255)
                                                                                      gcTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGt                                                  <  2:4500855/140‑1 (MQ=255)
                                                                                       cTGGCGAGAGAAGGGTTCAGGATCATAAGAGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAATCGTTGTGGCTAATCAATAAACTGGCTGATGCGCTGAATGGTc                                                 >  2:4220274/1‑140 (MQ=255)
                                                                                       cTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTc                                                 <  1:10536637/140‑1 (MQ=255)
                                                                                       cTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTc                                                 >  1:11052738/1‑140 (MQ=255)
                                                                                        tGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCa                                                <  1:1146404/140‑1 (MQ=255)
                                                                                        tGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCa                                                <  1:8981857/140‑1 (MQ=255)
                                                                                         ggCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCaa                                               >  1:6133113/1‑140 (MQ=255)
                                                                                            gagagaAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAat                                            <  2:127585/140‑1 (MQ=255)
                                                                                                 cagcctGCAGCATCAACTGCGCCTCCGTGAACCTGATCCCACCGCAAACAGGGCATCGTCTGAGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATAtgtg                                       <  2:10270737/135‑1 (MQ=255)
                                                                                                   gggTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCg                                     <  1:5841195/140‑1 (MQ=255)
                                                                                                      tGCAGGATCATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCATAATATGTGCGTTg                                  >  2:7137765/1‑140 (MQ=255)
                                                                                                              cATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGt                          <  1:6715762/140‑1 (MQ=255)
                                                                                                              cATAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGGCAAAATATGTGCGTTGGCATCCGt                          >  1:8836355/1‑140 (MQ=255)
                                                                                                               aTAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTg                         >  2:9432749/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGACTCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  1:9488999/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCGTCCGTgg                        >  2:6128095/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  2:6673340/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  1:6342503/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  2:903750/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  2:4047486/1‑140 (MQ=255)
                                                                                                                tAAGCGCCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTgg                        >  1:3183584/1‑140 (MQ=255)
                                                                                                                   gcgcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTCTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGgcgc                     <  1:272393/140‑1 (MQ=255)
                                                                                                                    cgcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCt                    <  1:7207783/140‑1 (MQ=255)
                                                                                                                     gcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTg                   >  2:187132/1‑140 (MQ=255)
                                                                                                                     gcCTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTg                   >  2:2853650/1‑140 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGa                  >  1:5490806/1‑140 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGa                  <  1:1378091/140‑1 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGa                  <  2:3505207/140‑1 (MQ=255)
                                                                                                                      ccTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATAAGTGCGTTGGCATCCGTGGCGCTGa                  >  1:6926291/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  2:6404584/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  2:5747768/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  1:2850359/1‑140 (MQ=255)
                                                                                                                       cTCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGAc                 >  2:5576730/1‑140 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACg                >  1:4700202/1‑140 (MQ=255)
                                                                                                                        tCCGTTAACCTGATACCAGCGCAAACAGAGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAACCGTAGAGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCAGACg                >  2:7316761/1‑140 (MQ=255)
                                                                                                                          cGTGAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCa              <  2:472334/140‑1 (MQ=255)
                                                                                                                            ttAACCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCaga            >  1:1585296/1‑140 (MQ=255)
                                                                                                                              aaCCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAgaga          <  1:9794505/140‑1 (MQ=255)
                                                                                                                               aCCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGaa         <  2:11441326/140‑1 (MQ=255)
                                                                                                                               aCCTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGaa         <  1:10425230/140‑1 (MQ=255)
                                                                                                                                 cTGATACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCGTCGGTGGCGCTGACGCTGAGAagc       >  1:6513863/1‑140 (MQ=255)
                                                                                                                                   gagaCCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcag     <  2:10118223/137‑1 (MQ=255)
                                                                                                                                    aTACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcagc    >  2:6578193/1‑140 (MQ=255)
                                                                                                                                    aTACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAagcagc    >  2:7025051/1‑140 (MQ=255)
                                                                                                                                     tACCAGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAAGCAGcc   <  2:4902415/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTGCTGTCCTCAATATCCAGATGCAGGCTCCCAAAATCCAGATTGTCCAATGCCGTCAGCTCACGACCATAGTGCAGAGGATTTGCGCTGGCGAGAGAAGGGTGCAGGATCATAAGCGCCTCCGTTAACCTGATACCGGCGCAAACAGTGCATCGTCTGTGCTGGCATTTCCCACTGCCGTTAAGCGTTGTGGCGAATCAATAAACTGGCTGATGCGCTGAATGGTCAAAATATGTGCGTTGGCATCCGTGGCGCTGACGCAGAGAAGCAGCCA  >  NZ_CP025268/4529340‑4529613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑