BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	442,101	T→C	197 (TAA→TAG)	yajL ←	protein deglycase YajL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	442,101	0	T	C	99.8%	1462.7 / NA	422	197 (TAA→TAG)	yajL	protein deglycase YajL
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (239/182); minor base A (0/1); total (239/183)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

tgtgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTa                                                                                                                                       >  2:3879990/1‑140 (MQ=255)

  tgAGCGCGGGGTGTTGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGCCactact                                                                                                                                     >  1:2525984/1‑140 (MQ=255)

  tgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTCTATCGCCCGTAATTTGTGGTTTGTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACactact                                                                                                                                     >  1:72773/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  1:8137166/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  1:8716887/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  1:6893059/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:709038/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTGTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:7653/1‑140 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTNGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  1:6182980/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCTTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  2:3098231/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  1:4311862/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  1:2163705/1‑140 (MQ=255)

             tgatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATta                                                                                                                          <  1:7545614/140‑1 (MQ=255)

               atgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATtata                                                                                                                        <  1:8757997/140‑1 (MQ=255)

                 gaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATaaa                                                                                                                      <  1:657633/140‑1 (MQ=255)

                  cgCCGTCTGCAGGCGCACTATCTGCGCGAGCAGGGCTTTAACACTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCAACGGGTCAGAGGCGACGTTTGCCGCATCCGACACTACTCGTAATACTGATAAAt                                                                                                                     <  2:1143086/139‑1 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGGTTGCCGCATCCGACACTACTCGTAATAATTATAAATccc                                                                                                                  >  2:3480864/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                <  1:6418868/140‑1 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                <  2:2262629/140‑1 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  1:4291732/1‑140 (MQ=255)

                         tGCAGGCGCGCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTACTTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCACCCGACACTACTCGTAATCATTATAAATCCCTgcc                                                                                                              >  1:4522630/1‑140 (MQ=255)

                          gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccg                                                                                                             <  2:913160/140‑1 (MQ=255)

                            aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                           <  2:2416728/140‑1 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:1557863/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      <  1:7322119/140‑1 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:9655482/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:2384365/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGATTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      <  1:5665671/140‑1 (MQ=255)

                                 ctcgATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAAGTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACGACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:4415772/1‑140 (MQ=255)

                                      tCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGACAGAGGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGtt                                                                                                 <  2:2195804/140‑1 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  2:6585264/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCAGTAATTTGTGGTGTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGa                                                                                             >  2:2637970/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTAAACAATGTGAAGGTATATCGCCCGTAATTTGTGGGTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  2:1732489/1‑140 (MQ=255)

                                           gcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGAt                                                                                            >  1:10438540/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                           <  1:4133179/140‑1 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATg                                                                                           >  1:1036398/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGACGCCATCACCAGTTGTGATg                                                                                           >  1:11538097/1‑140 (MQ=255)

                                            cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGGAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGGTTGCCGCATCCGACACTACTCGTAATAATTATAACTCCCTGCCGCCATCACCAGTTGTGATg                                                                                           >  2:4675841/1‑140 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                         >  2:1894442/1‑140 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                         >  1:1076230/1‑140 (MQ=255)

                                                cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACCCTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                                                                                       >  1:8345659/1‑140 (MQ=255)

                                                cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                                                                                       >  1:3003904/1‑140 (MQ=255)

                                                    gCTTTAACACTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                                   <  2:6389996/140‑1 (MQ=255)

                                                     cTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCAt                                                                                  <  2:7225036/140‑1 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:6009837/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:3710238/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCCTCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  1:10195773/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  1:7881457/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  2:2116415/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  2:11187980/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  1:10880607/1‑140 (MQ=255)

                                                                tgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCa                                                                       >  1:3885556/1‑140 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  2:592560/140‑1 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  1:2906014/140‑1 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGATTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  1:9084925/140‑1 (MQ=255)

                                                                   aGGTATATCGCCCGTAAATTGAGGATTTTACGACGCATCTGGTCAGAAGCGACGTTTGCCGCAGCCGACACTACTCGTAAGAATTATAAATCCCTGCCCCCATCACCAGTTGTGATGCCACATCATGGGCTATTACACGc                                                                    <  2:360862/140‑1 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  2:2824870/1‑140 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  1:11144218/1‑140 (MQ=255)

                                                                         atCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacc                                                              >  1:9420047/1‑140 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           >  2:4836902/1‑140 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           <  2:8217807/140‑1 (MQ=255)

                                                                              ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAg                                                         >  1:2766620/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCACTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCACAACCAACAgg                                                        >  2:8831784/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       <  2:6978796/140‑1 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       >  1:1661949/1‑140 (MQ=255)

                                                                                     ttGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                                  <  1:5371038/140‑1 (MQ=255)

                                                                                       gtgGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCAACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAt                                                >  1:4802063/1‑140 (MQ=255)

                                                                                        tgGGTTTTACGTCGCTTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACGTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtt                                               >  2:7425107/1‑140 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACCGGTCGATAAtttt                                             <  2:9783637/140‑1 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  1:1894442/140‑1 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  2:501464/140‑1 (MQ=255)

                                                                                             tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAg                                          >  1:6043070/1‑140 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:813932/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:8528054/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  1:3262231/140‑1 (MQ=255)

                                                                                                   tCGCATCTGGTGAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATTATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCTCGCCCAACCAACCGGTCGATAATTTTCAGACCaat                                    <  2:8413965/140‑2 (MQ=255)

                                                                                                   tCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCaaa                                    <  2:4291732/140‑1 (MQ=255)

                                                                                                     gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGt                                  >  1:9768923/1‑140 (MQ=255)

                                                                                                      cATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAAGTATAAATCCCTGCCGCCATCACCATTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTc                                 <  1:9884253/140‑1 (MQ=255)

                                                                                                       aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCCACAGGTCGATAATTTTCAGACCAAAGTCg                                <  2:1036983/140‑1 (MQ=255)

                                                                                                       aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCg                                <  1:5514470/140‑1 (MQ=255)

                                                                                                          tGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATa                             <  1:7016474/140‑1 (MQ=255)

                                                                                                                gATGCGACGTTTGCCGCATCCTACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACACACGTCGATAGCTGTa                       >  2:1778539/1‑140 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:10189740/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:9480339/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:7979318/140‑1 (MQ=255)

                                                                                                                   gCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                    >  1:1394155/1‑140 (MQ=255)

                                                                                                                   gCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                    <  2:8322701/140‑1 (MQ=255)

                                                                                                                   gCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                    <  1:10010412/140‑1 (MQ=255)

                                                                                                                      aCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGc                 >  2:1425819/1‑140 (MQ=255)

                                                                                                                       cGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGcc                <  1:5105701/140‑1 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  2:8064764/1‑140 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  1:7750565/1‑140 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTGGGTCACGCCCAACCAACCGGTCGATCATTTTCAGACCAATGTCGAGGGCTGTACCCGGCCCctg            >  1:8624356/1‑140 (MQ=255)

                                                                                                                                cATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggctgg       >  2:5374929/1‑140 (MQ=255)

                                                                                                                                 aTCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt      >  2:10900874/1‑140 (MQ=255)

                                                                                                                                 aTCCGACACTACTCGTAATAATTATAAATCCCCGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt      <  1:9298377/140‑1 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAACTCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACCGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  2:7850305/140‑1 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  2:4121337/140‑1 (MQ=255)

                                                                                                                                     gACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGc  <  1:5016032/140‑1 (MQ=255)

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`