BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	449,814	T→C	T283A (ACT→GCT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	449,814	0	T	C	100.0%	2694.0 / NA	770	T283A (ACT→GCT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (521/249); total (521/249)

Only 100 of 770 total aligned reads displayed.

TAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCT  >  NZ_CP025268/449677‑449950

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                        >  2:11597588/1‑140 (MQ=255)

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                        >  1:5331155/1‑140 (MQ=255)

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCAGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTGCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                        >  2:9551415/1‑140 (MQ=255)

 aaTTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCAt                                                                                                                                       <  1:4372244/140‑1 (MQ=255)

 aaTTGTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCAt                                                                                                                                       <  1:7136639/140‑1 (MQ=255)

  aTTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATa                                                                                                                                      <  1:1406628/140‑1 (MQ=255)

      tCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACt                                                                                                                                  >  1:6206064/1‑140 (MQ=255)

      tCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCGGGGTCATGTCCATGCTCTTAACGTGAGCCCTAAACt                                                                                                                                  >  2:8334696/1‑140 (MQ=255)

                    ttcttcCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCg                                                                                                                    <  1:3309580/140‑1 (MQ=255)

                         ccacAACCCCGTTCATGGGCGGATTCCGCGTGGCTCATGTCCAGGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCAATGCTCTTACCGTGAGCCATAATCTTGTTAATTACATCGGCtaa                                                                                                               >  2:2344260/4‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                               >  1:3918845/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                               >  2:3313815/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGATCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                               >  2:9113332/1‑140 (MQ=255)

                          cTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAAc                                                                                                              >  2:1560672/1‑140 (MQ=255)

                           tCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACa                                                                                                             <  1:6603269/140‑1 (MQ=255)

                                 ccTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTg                                                                                                       >  1:10421236/1‑140 (MQ=255)

                                   tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGt                                                                                                     >  2:580775/1‑140 (MQ=255)

                                   tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGt                                                                                                     >  1:2146418/1‑140 (MQ=255)

                                        tGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCAc                                                                                                <  1:4670076/140‑1 (MQ=255)

                                        tGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCAc                                                                                                <  2:5645940/140‑1 (MQ=255)

                                          ggCGGAGTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCAGGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                              <  1:10008354/140‑1 (MQ=255)

                                           gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGtt                                                                                             >  1:635234/1‑140 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                          <  1:563694/126‑1 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                            <  2:8184307/140‑1 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                            <  1:781218/140‑1 (MQ=255)

                                                ttCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGgaga                                                                                        <  2:7897168/140‑1 (MQ=255)

                                                 tCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGaa                                                                                       <  2:8754199/140‑1 (MQ=255)

                                                    gcgTGGCTCATGTCCATGCCTTCCCTACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAata                                                                                    <  1:9631874/140‑1 (MQ=255)

                                                         gCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTACa                                                                               >  1:1821442/1‑140 (MQ=255)

                                                          cTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGATCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc                                                                              <  2:6303374/140‑1 (MQ=255)

                                                              tGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                          <  2:4047827/140‑1 (MQ=255)

                                                              tGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                          <  2:3720305/140‑1 (MQ=255)

                                                                   aagCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTATTTACAACGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTa                                                                     >  2:9736634/3‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACACGTCTGGTTTCACGGTGGAGAAATATTCCACCTGGTTGTAt                                                                    >  1:11129342/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                    >  2:6800087/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                    >  1:6941804/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTGGTAt                                                                    >  1:8072907/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCGGGTTGTAt                                                                    >  1:2735476/1‑140 (MQ=255)

                                                                       cTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCg                                                                 >  1:2272999/1‑140 (MQ=255)

                                                                        ttCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGc                                                                >  1:375355/1‑140 (MQ=255)

                                                                        ttCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGc                                                                <  1:5872820/140‑1 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                               >  2:10788943/1‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                               >  1:9718717/1‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAGTCGCt                                                               >  2:11033830/1‑140 (MQ=255)

                                                                         tCCATACCGTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCAAACACTTGTTAATTACATCGGCAAACACGTCTGTTTTCACGATGGAGAAATCTTCCAACTGGGAGTATTCGca                                                               >  2:725992/1‑139 (MQ=255)

                                                                          ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTa                                                              >  1:4502593/1‑140 (MQ=255)

                                                                          ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCAATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTa                                                              >  2:2230079/1‑140 (MQ=255)

                                                                           cATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAg                                                             >  2:3574177/1‑140 (MQ=255)

                                                                                  tCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGc                                                      >  2:10400148/1‑140 (MQ=255)

                                                                                  tCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGc                                                      >  1:1086744/1‑140 (MQ=255)

                                                                                   cGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGcc                                                     <  2:9869079/140‑1 (MQ=255)

                                                                                    gtgtGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCa                                                    >  2:6047998/1‑140 (MQ=255)

                                                                                     tgtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAg                                                   >  2:11420368/1‑140 (MQ=255)

                                                                                      gtgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGt                                                  >  1:11402522/1‑140 (MQ=255)

                                                                                         cgcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGtttt                                               <  1:4980264/140‑1 (MQ=255)

                                                                                          gcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACTTCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTCTTCGCTAGGCGCGGCCAGttttt                                              >  2:7466195/1‑140 (MQ=255)

                                                                                          gcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCAGGTTGTATTCGCTCGGCGCGGCCAGttttt                                              >  2:4660259/1‑140 (MQ=255)

                                                                                              tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTATGCGCGGCCAGTTTTTCGaa                                          >  2:10517521/1‑140 (MQ=255)

                                                                                               gCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCACACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGacc                                         <  1:6783711/140‑3 (MQ=255)

                                                                                                cTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACg                                        >  1:738022/1‑140 (MQ=255)

                                                                                                  cACCTTCTGCCTGGCTCATGACCATGCTCATACCGTGAGCCATCAACTTTTTAATTACATCGGCACACAAGTCATGTGTCACGATGGAGAAATATTACACCTGTTCGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCa                                      <  2:658065/140‑1 (MQ=255)

                                                                                                   cccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                     <  2:7105667/139‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                     <  2:1350301/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                     <  2:4886861/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                     <  1:7633077/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCAGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAAGTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATAGTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                     <  1:8983833/140‑1 (MQ=255)

                                                                                                     cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGcc                                   <  2:5300459/140‑1 (MQ=255)

                                                                                                     cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGcc                                   <  1:2946513/140‑1 (MQ=255)

                                                                                                     cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGcc                                   <  1:10189274/140‑1 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                                >  1:2585545/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                                >  1:3411515/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                                >  1:8740853/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                                >  1:8348893/1‑140 (MQ=255)

                                                                                                          ggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTc                              <  1:3549438/140‑1 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                         >  1:9964094/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                         >  1:5469140/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                         >  2:1998364/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                         >  1:7960671/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAAATTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                         >  2:2703530/1‑140 (MQ=255)

                                                                                                                gTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACAt                        <  2:1017879/140‑1 (MQ=255)

                                                                                                                 tCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGAGAGGCCAGTTTTTCGATCGCAGCCATGTCAGACATg                       >  2:7568087/1‑140 (MQ=255)

                                                                                                                  cATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATgg                      >  2:11192379/1‑140 (MQ=255)

                                                                                                                    tGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGgtg                    <  1:6069515/140‑1 (MQ=255)

                                                                                                                     gTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGATCGCAGCCATGTCAGACATGgtgt                   >  2:7774167/1‑140 (MQ=255)

                                                                                                                     gTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGgtgt                   >  2:5047213/1‑140 (MQ=255)

                                                                                                                         aTGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCgg               >  2:5550902/1‑140 (MQ=255)

                                                                                                                          tGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGc              >  2:4399242/1‑140 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg             >  1:9181438/1‑140 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg             <  1:9478698/140‑1 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg             >  1:3032539/1‑140 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg             >  1:6431580/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                              <  1:1201100/54‑1 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc        >  1:4176046/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc        >  1:4886351/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc        >  2:4506329/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGCTCGGCGACTGc        >  1:1468854/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAACTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc        >  2:2921963/1‑140 (MQ=255)

                                                                                                                                 aCCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAGATATTCCACATGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCt       >  2:1720454/1‑140 (MQ=255)

                                                                                                                                    gTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCg    >  2:3069484/1‑140 (MQ=255)

                                                                                                                                     tGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGc   <  2:9359388/140‑1 (MQ=255)

TAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCT  >  NZ_CP025268/449677‑449950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`