Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 489,335 T→C *176* (TAA→TAG)  priC ← primosomal replication protein N''

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268489,3350TC99.7% 2379.4 / NA 681*176* (TAA→TAG) priCprimosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (424/255);  minor base A (2/0);  total (426/255)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 681 total aligned reads displayed.

CATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489198‑489471
                                                                                                                                         |                                                                                                                                        
 aTAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAg                                                                                                                                       <  1:10897132/140‑1 (MQ=255)
 aTAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAg                                                                                                                                       <  2:5190569/140‑1 (MQ=255)
         gcattACAATATCCAGCGCGCGCAGCACGGTACGGGCAGGGATCTGATTTTCCTCCAGCAGCACACTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTaa                                                                                                                               <  2:8067254/137‑1 (MQ=255)
                 atatCCAGCGCGCGCAGCACGGGACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTa                                                                                                                       <  1:11226594/140‑1 (MQ=255)
                   atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAc                                                                                                                     >  2:5536249/1‑140 (MQ=255)
                       cgcgcgcgcAGCACGGTACTGGCCGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTGAGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATAGCTAGCGGGTTAAACGCGCTCACCTGt                                                                                                                 >  2:180432/2‑140 (MQ=255)
                         cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:4217982/1‑140 (MQ=255)
                         cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:2197578/1‑140 (MQ=255)
                         cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:4443978/1‑140 (MQ=255)
                         cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:1806524/1‑140 (MQ=255)
                         cgcgcgcAGCAAGGTACTGGCAGGGATCTGAGTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCCGGGGCATTTTCCCGTGACAGATTCTCTCCATTGCTAGCGGGTTAAACGCGCTACCCTGttt                                                                                                               >  2:3488177/1‑140 (MQ=255)
                            gcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCg                                                                                                            >  1:10405979/1‑140 (MQ=255)
                              gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAt                                                                                                          >  2:4283653/1‑140 (MQ=255)
                              gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAt                                                                                                          >  2:8453499/1‑140 (MQ=255)
                                  caCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAAACAACTCGACAGCCAGTTTGACAGCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAatttt                                                                                                      >  2:8810469/1‑140 (MQ=255)
                                   ccGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAACTCGACCGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAtttttt                                                                                                     <  1:6561821/139‑1 (MQ=255)
                                    cGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc                                                                                                    <  1:9623911/140‑1 (MQ=255)
                                       tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg                                                                                                 <  2:8914994/140‑1 (MQ=255)
                                           ggCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                             <  1:7627677/140‑1 (MQ=255)
                                           ggCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                             <  2:4094769/140‑1 (MQ=255)
                                           ggCAGGGATCTGATTGTCCTCCAGCAGCACACTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCGAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                             <  2:7875800/140‑1 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACCTAGTCTCTCCCTTGCTCGCGGGTTAAACGCGAGAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  2:9531646/1‑140 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAATCGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  2:4478484/1‑140 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:4182068/1‑140 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:11362071/1‑140 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:11550348/1‑140 (MQ=255)
                                            gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  2:3941963/1‑140 (MQ=255)
                                              aGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCAGTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCCTgg                                                                                          >  2:2721428/1‑140 (MQ=255)
                                               gggATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggc                                                                                         >  2:2495667/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACATCCAGTTTGACATCGTCAGGGGCATTTTCCATTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:5364019/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:9426236/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:9830543/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:8703041/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:9350320/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:7144436/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:378652/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:5480351/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:4983829/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:3224391/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:2887808/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:6584999/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:1929379/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:10195164/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGGTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:11147337/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCGGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:3175379/1‑140 (MQ=255)
                                                 gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCAATTGCTAGCGGGTTAAACGCGCTAACATGTTTTCGATTTTTTCCAGCGCATtgcgg                                                                                       >  1:6311121/1‑140 (MQ=255)
                                                 gATCTGAGTGTCCTCCAGCAGCACAATCAAATCGACAGCCCGTTTGACCTCGTCAGGGGCCTTGTCCAGTGTCCTATGCTCGCCATTGCTAGCGGGTTAAAAGCGCTCCCCGGGGTTCGCTGTTTTCCAGCGCCTggcgg                                                                                       >  2:10177551/1‑140 (MQ=255)
                                                 gATCTGAATTTCCTCCATCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTATCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTACATAGCATggcgg                                                                                       >  2:10909813/1‑140 (MQ=255)
                                                    cTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                                    <  2:566466/140‑1 (MQ=255)
                                                    cTGACTTTCCTCCACCAGCACAATCAAAGCGACAGCCAGTTTGACATCGACAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTATCGGGTTAAACGCGCTAACCTGTGTTCTATTTTTTCCAGCGCATGGCGGCAg                                                                                    <  2:5838979/140‑1 (MQ=255)
                                                      gATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCg                                                                                  >  1:6087468/1‑140 (MQ=255)
                                                         tttCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGc                                                                               <  1:8541291/140‑1 (MQ=255)
                                                          ttCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGcc                                                                              >  2:1123760/1‑140 (MQ=255)
                                                            cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg                                                                            >  1:3670796/1‑140 (MQ=255)
                                                            cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg                                                                            >  2:1917143/1‑140 (MQ=255)
                                                             cgccAGCAGCACACTCAAAGCCACAGCCAGATTGACACCGTCCGGGGCAGTTTCCCGAGACATAGTCTCTCCAGTGCGAGAGGGTTTCACGCGCTAACCGGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAgg                                                                           <  2:7737637/138‑1 (MQ=255)
                                                               ccAGCAGCACATTCAAATCGACAGCAAGTTTGACATCGTCAGGGGCATTTTCCAGGGACATATTATCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  1:10022236/140‑1 (MQ=255)
                                                               ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  1:5286243/140‑1 (MQ=255)
                                                               ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  1:1405111/140‑1 (MQ=255)
                                                               ccAGCAGCACAATCAAATCGACAGCCAGTTTGACAACGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATAGCTAGCGGGTTAAACCCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:488092/140‑1 (MQ=255)
                                                                 agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       <  2:3075697/140‑1 (MQ=255)
                                                                 agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTGTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       <  2:971932/140‑1 (MQ=255)
                                                                      cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  1:7802160/140‑1 (MQ=255)
                                                                      cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  1:3843430/140‑1 (MQ=255)
                                                                      cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  1:4570233/140‑1 (MQ=255)
                                                                        caATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATa                                                                <  1:9104934/140‑1 (MQ=255)
                                                                           tCATATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGc                                                             <  2:610907/140‑1 (MQ=255)
                                                                              aaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTc                                                          <  2:232896/140‑1 (MQ=255)
                                                                                   aCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACtt                                                     >  1:7302415/1‑140 (MQ=255)
                                                                                     aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   >  2:11614284/1‑140 (MQ=255)
                                                                                     aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   >  1:6787641/1‑140 (MQ=255)
                                                                                     aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   <  2:10721739/140‑1 (MQ=255)
                                                                                       caaGTTTGACATCGTAAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTTCCAGGCTCGCTTCATAGGCTTCCACTTCACg                                                 >  1:2449513/3‑140 (MQ=255)
                                                                                           tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGc                                             >  2:6858024/1‑140 (MQ=255)
                                                                                              gACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGc                                          <  1:10627592/140‑1 (MQ=255)
                                                                                                 aTCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGtt                                       >  1:8905843/1‑140 (MQ=255)
                                                                                                   cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTg                                     >  1:1683304/1‑140 (MQ=255)
                                                                                                   cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTg                                     <  2:8258938/140‑1 (MQ=255)
                                                                                                    gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCGAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGc                                    <  1:2208891/140‑1 (MQ=255)
                                                                                                     tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         >  1:7548214/1‑102 (MQ=255)
                                                                                                     tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:7547942/102‑1 (MQ=255)
                                                                                                     tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCt                                   >  1:5708966/1‑140 (MQ=255)
                                                                                                     tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCt                                   >  1:3407652/1‑140 (MQ=255)
                                                                                                      cAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                                  <  2:5970620/140‑1 (MQ=255)
                                                                                                         gggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTc                               >  2:10300032/1‑140 (MQ=255)
                                                                                                          ggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTcc                              >  2:7657200/1‑140 (MQ=255)
                                                                                                           gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCa                             <  2:5045770/140‑1 (MQ=255)
                                                                                                               tttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACgag                         <  2:1568448/140‑1 (MQ=255)
                                                                                                               tttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACgag                         >  1:8033193/1‑140 (MQ=255)
                                                                                                               tttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACgag                         >  1:5826449/1‑140 (MQ=255)
                                                                                                                 tCCTGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGAt                       <  2:7271730/140‑1 (MQ=255)
                                                                                                                    aGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCgg                    >  1:8220864/1‑140 (MQ=255)
                                                                                                                        aCATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAc                <  2:10631521/140‑1 (MQ=255)
                                                                                                                        aCATATTCTCTCCAGTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATATTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAc                <  1:11104976/140‑1 (MQ=255)
                                                                                                                              tctctcCAGTGCTAGCGGGTTAAACGCGCTAACCAGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCCTAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCgcgc          <  2:9145476/140‑1 (MQ=255)
                                                                                                                                tctcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTGTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCa        >  2:6413261/1‑140 (MQ=255)
                                                                                                                                  tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:2767063/1‑140 (MQ=255)
                                                                                                                                  tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      <  2:1357992/140‑1 (MQ=255)
                                                                                                                                  tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      <  1:4713100/140‑1 (MQ=255)
                                                                                                                                  tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:8373231/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489198‑489471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑