BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,331,820	(G)_7→6	pseudogene (473/817 nt)	rdcB →	YjcZ‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,331,814	0	G	.	99.0%	3459.2 / 16.1	727	pseudogene (467/817 nt)	rdcB	YjcZ‑like family protein
Reads supporting (aligned to +/- strand): ref base G (1/6); new base . (311/407); total (313/414)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 747 total aligned reads displayed.

GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949

  aaaGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTggg                                                                                                                                       <  1:10322895/140‑1 (MQ=255)

           aaCGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTg                                                                                                                             >  2:3186366/1‑140 (MQ=255)

             cGCAACGCGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTCTCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGc                                                                                                                           <  1:1549942/140‑1 (MQ=255)

             cGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGc                                                                                                                           >  2:7496877/1‑140 (MQ=255)

              gCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCg                                                                                                                          <  2:4492840/140‑1 (MQ=255)

                 aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtg                                                                                                                       >  1:9579140/1‑140 (MQ=255)

                 aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtg                                                                                                                       >  1:2487458/1‑140 (MQ=255)

                 aCGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtg                                                                                                                       >  1:6547348/1‑140 (MQ=255)

                      gCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcctgc                                                                                                                  <  1:9718716/140‑1 (MQ=255)

                       ccTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGAtgcctgcc                                                                                                                 <  2:8644384/140‑1 (MQ=255)

                        cTACTGGTGCAGCCAGGTGATCAGAGCGAGCAAATTTTTTCCCGCTGGCGTGCTGGGTCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCg                                                                                                                <  2:10985055/140‑1 (MQ=255)

                          aCTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTc                                                                                                              >  2:5019907/1‑140 (MQ=255)

                               tGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGa                                                                                                         <  2:1356194/140‑1 (MQ=255)

                                gcaggcagGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGaa                                                                                                        >  1:2667429/1‑140 (MQ=255)

                                gcaggcagGTGATCAGAGCGAGCAAATTTTTACCCGCTGGAGTGCTGGGGAGTATAACGCGTGGTAGTTGGATGTGCGCTGTAGTAGCGATATGGTGGAGTTGCGCT‑GGGGGGCGTTTGGGGATGCATGCGGTCTggggg                                                                                                        >  2:9598457/1‑138 (MQ=255)

                                 caggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAg                                                                                                       <  1:5647316/140‑1 (MQ=255)

                                   ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  2:8847037/1‑140 (MQ=255)

                                   ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  1:4825064/1‑140 (MQ=255)

                                   ggcaggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGcc                                                                                                     >  1:3663332/1‑140 (MQ=255)

                                     caggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCg                                                                                                   >  1:9944819/1‑140 (MQ=255)

                                      aggTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGc                                                                                                  >  1:9035235/1‑140 (MQ=255)

                                        gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:2248780/1‑140 (MQ=255)

                                        gTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCaa                                                                                                >  1:5830711/1‑140 (MQ=255)

                                         tGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGGTTGATCGTGCTGGCGGAGGTGCGCT‑GGGGGGCATTGGGCGATGCCTGCCGTCTGGGAAGCCCGCAAg                                                                                               <  2:414027/140‑1 (MQ=255)

                                            tCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCgg                                                                                            <  1:2916276/140‑1 (MQ=255)

                                              agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          >  1:3444369/1‑140 (MQ=255)

                                              agagCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTg                                                                                          >  1:5140392/1‑140 (MQ=255)

                                                agcgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGc                                                                                        >  2:913344/1‑140 (MQ=255)

                                                  cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:5978021/1‑140 (MQ=255)

                                                  cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  2:2569689/1‑140 (MQ=255)

                                                  cgagcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgt                                                                                      >  1:6281801/1‑140 (MQ=255)

                                                     gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  1:6105659/1‑140 (MQ=255)

                                                     gcAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgt                                                                                   >  1:6827313/1‑140 (MQ=255)

                                                      cAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCgttgtt                                                                                  >  1:11444894/1‑140 (MQ=255)

                                                        aatgagtACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGc                                                                                <  1:7234813/134‑1 (MQ=255)

                                                                cccGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGAt                                                                        <  1:7796243/140‑1 (MQ=255)

                                                                   gCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTg                                                                     >  1:2771428/1‑140 (MQ=255)

                                                                   gCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTg                                                                     >  1:931881/1‑140 (MQ=255)

                                                                      ggCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTc                                                                  <  1:8187179/140‑1 (MQ=255)

                                                                          tGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCgaga                                                              <  2:2793826/140‑1 (MQ=255)

                                                                           gCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGTCGATGACTGCAGTCTGGTAAGCCCGCAAGCGTTGGCGTTGTTGCTGGGTGATTTGCTCGTGaa                                                             <  1:4135863/140‑1 (MQ=255)

                                                                             tGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAg                                                           <  1:497109/140‑1 (MQ=255)

                                                                               gggCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCg                                                         >  2:3310252/1‑140 (MQ=255)

                                                                                 gCGTAGAACGCCGGGTCGGTGCCTGGGCGCTGTGTTATCGTGCTGGCGGAGTTGCGCT‑GGGGGGCATTTGGCGAGGCCGGCCGGCTGGGAAGCCCGCAAGCGGTGGCGTGGTTGCTGGGGGATTTGCTCGAGAAAGCgcc                                                       <  2:4328682/140‑3 (MQ=255)

                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  1:8823055/1‑140 (MQ=255)

                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  1:4374407/1‑140 (MQ=255)

                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    >  2:2095265/1‑140 (MQ=255)

                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGAcaca                                                    <  1:1785166/140‑1 (MQ=255)

                                                                                    tataACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGGTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTGGCTCGAGAAAGCGAcaca                                                    <  2:8135627/140‑1 (MQ=255)

                                                                                        aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                                                <  1:10183949/140‑1 (MQ=255)

                                                                                        aCGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACAt                                                <  1:7635510/140‑1 (MQ=255)

                                                                                         cGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATc                                               >  1:1001214/1‑140 (MQ=255)

                                                                                            cTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTgg                                            >  2:2910326/1‑140 (MQ=255)

                                                                                             tGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGc                                           <  1:1160992/140‑1 (MQ=255)

                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          <  1:7479943/140‑1 (MQ=255)

                                                                                              ggTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCa                                          >  2:1454197/1‑140 (MQ=255)

                                                                                               gTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGAAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCag                                         >  2:3730993/1‑140 (MQ=255)

                                                                                                tCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga                                        <  1:5573731/140‑1 (MQ=255)

                                                                                                tCGTTGCCTGGGCGCTGATGTATCGTTCGGGAGGAGGTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCaga                                        <  2:11236711/140‑1 (MQ=255)

                                                                                                 cGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCagag                                       <  1:7986747/140‑1 (MQ=255)

                                                                                                   ttGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCagagag                                     >  2:356038/1‑140 (MQ=255)

                                                                                                    tGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGt                                    <  2:4934847/140‑1 (MQ=255)

                                                                                                      ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  1:854462/140‑1 (MQ=255)

                                                                                                      ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:9451556/140‑1 (MQ=255)

                                                                                                      ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:11334370/140‑1 (MQ=255)

                                                                                                      ccTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  >  1:5982372/1‑140 (MQ=255)

                                                                                                      ccTGGGCGCTGTATTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGATGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTAt                                  <  2:9748089/140‑1 (MQ=255)

                                                                                                         gggCGCTGTTTTATCGTTCGGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCCAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACCACATCTGGCAGAGAGTATCaa                               >  2:532127/1‑140 (MQ=255)

                                                                                                          ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              <  2:7095485/140‑1 (MQ=255)

                                                                                                          ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  1:11194864/1‑140 (MQ=255)

                                                                                                          ggCGCTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAAt                              >  2:7231343/1‑140 (MQ=255)

                                                                                                             gcTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCg                           <  2:1806994/140‑1 (MQ=255)

                                                                                                              cTGTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCgg                          >  1:3828070/1‑140 (MQ=255)

                                                                                                                gTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCa                        <  1:2109307/140‑1 (MQ=255)

                                                                                                                gTTTTATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCa                        >  2:7718210/1‑140 (MQ=255)

                                                                                                                    tATCGTTCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCa                        >  2:8336847/1‑136 (MQ=255)

                                                                                                                         ttCTGGAGGAGTTGCGCT‑TGGGGTCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGAAGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCATTGCGGCACCGAcgaag               >  2:638654/1‑136 (MQ=255)

                                                                                                                         ttCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcg               >  1:1204148/1‑140 (MQ=255)

                                                                                                                          tCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgc              <  2:6827312/140‑1 (MQ=255)

                                                                                                                          tCTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGGTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgc              <  2:1024162/140‑1 (MQ=255)

                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  2:6269306/1‑140 (MQ=255)

                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  2:7968529/140‑1 (MQ=255)

                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             >  1:11037033/1‑140 (MQ=255)

                                                                                                                           cTGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCAcgcg             <  1:10733742/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:6568636/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:4641360/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:3175122/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:711168/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  1:8678436/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:3986179/140‑1 (MQ=255)

                                                                                                                            tGGAGGAGTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGt            <  2:11570053/140‑1 (MQ=255)

                                                                                                                              gaggagTTGCGCT‑GGGGGGCTTTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGTTGTCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGTCACATCATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:5362094/140‑1 (MQ=255)

                                                                                                                              gaggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCa          <  1:9167863/140‑1 (MQ=255)

                                                                                                                                ggagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACt        <  2:10589397/140‑1 (MQ=255)

                                                                                                                                 gagTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGGTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTa       <  1:10575621/140‑1 (MQ=255)

                                                                                                                                   gTTGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAtt     <  1:645034/140‑1 (MQ=255)

                                                                                                                                    ttGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTa    >  2:238442/1‑140 (MQ=255)

                                                                                                                                     tGCGCT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTAc   <  2:5603966/140‑1 (MQ=255)

                                                                                                                                      gcgcT‑GGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTAATAcc  >  2:5874845/1‑140 (MQ=255)

                                                                                                                                       gcgcgggggggCATTTGGCGATGCCTGCCGTCTTGGAAGACCGCACGCGGTGGCGTTGTTGCTGTGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGATTATCAATGAGGCACCGACCACGCGTCACTATTAcc  <  1:1318745/136‑1 (MQ=255)

GAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGGAGTTGCGCTGGGGGGGCATTTGGCGATGCCTGCCGTCTGGGAAGCCCGCAAGCGGTGGCGTTGTTGCTGGGTGATTTGCTCGAGAAAGCGACACAACATCTGGCAGAGAGTATCAATGCGGCACCGACCACGCGTCACTATTACC  >  NZ_CP025268/4331675‑4331949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`