BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,013,520	+G	pseudogene (463/542 nt)	zapC →	cell division protein ZapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,013,518	1	.	G	98.1%	2025.0 / 25.0	592	pseudogene (461/542 nt)	zapC	cell division protein ZapC
Reads supporting (aligned to +/- strand): ref base . (5/6); new base G (293/288); total (298/294)
Fisher's exact test for biased strand distribution p-value = 7.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 631 total aligned reads displayed.

ATGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑G‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NZ_CP025268/1013379‑1013651

 tGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g                                                                                                                                        >  1:5314848/1‑140 (MQ=255)

 tGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g                                                                                                                                        >  1:11314348/1‑140 (MQ=255)

    cGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g‑ggc                                                                                                                                    >  2:11137318/1‑138 (MQ=255)

     gggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g‑ggcg                                                                                                                                   >  1:5105662/1‑137 (MQ=255)

      ggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAGATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGTTGCTGT‑T‑g‑ggcgt                                                                                                                                  >  1:7214302/1‑136 (MQ=255)

      ggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g‑ggcga                                                                                                                                  >  1:10279052/1‑136 (MQ=255)

       gCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATATGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑g‑ggcgac                                                                                                                                 >  1:1941498/1‑135 (MQ=255)

        cGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACg                                                                                                                                >  1:9430427/1‑140 (MQ=255)

        cGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACg                                                                                                                                >  1:37565/1‑140 (MQ=255)

          aTGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCCGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCCCTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCCGT‑TGG‑G‑CGACggc                                                                                                                              >  2:9148435/1‑138 (MQ=255)

           tGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCa                                                                                                                             >  1:9580921/1‑140 (MQ=255)

           tGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCa                                                                                                                             >  2:5059824/1‑140 (MQ=255)

                        tGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGaa                                                                                                                >  2:5888852/1‑140 (MQ=255)

                          gTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACg                                                                                                              >  1:3162051/1‑140 (MQ=255)

                           ttGAGCGATACTCACGAGCAGGTCAATGTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGa                                                                                                             <  2:4199071/140‑1 (MQ=255)

                              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:6946696/1‑140 (MQ=255)

                              aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAg                                                                                                          >  1:10700903/1‑140 (MQ=255)

                                 gATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCt                                                                                                       <  1:3330350/140‑1 (MQ=255)

                                 gATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCt                                                                                                       <  1:1558782/140‑1 (MQ=255)

                                   tACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGa                                                                                                     <  1:8308985/140‑1 (MQ=255)

                                    aCTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGaa                                                                                                    >  1:4496230/1‑140 (MQ=255)

                                    aCTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGaa                                                                                                    >  1:4322671/1‑140 (MQ=255)

                                       cACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAAcc                                                                                                 <  2:4978489/140‑1 (MQ=255)

                                        ccGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTTTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCTTGAACGACAGGCTGAAACCg                                                                                                <  1:5531648/139‑1 (MQ=255)

                                        aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:7201767/140‑1 (MQ=255)

                                        aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                >  1:10401830/1‑140 (MQ=255)

                                        aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:8280771/140‑1 (MQ=255)

                                          gAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGGTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCa                                                                                              >  1:2145791/1‑140 (MQ=255)

                                             cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGATCGACAGGCTGAAACCGCAGGt                                                                                           >  2:11275993/1‑140 (MQ=255)

                                             cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:2089921/1‑140 (MQ=255)

                                               ggTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         >  2:5297248/1‑140 (MQ=255)

                                                gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:10016882/1‑140 (MQ=255)

                                                gTCAATTTGCTGGTCGGTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTat                                                                                        >  2:1259808/1‑139 (MQ=255)

                                                       tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCTGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  2:10836208/140‑1 (MQ=255)

                                                       tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:11138622/140‑1 (MQ=255)

                                                       tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:9139904/140‑1 (MQ=255)

                                                       tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:8569727/140‑1 (MQ=255)

                                                          tGGTCGTTGAATCTGGCGAACATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGc                                                                              >  2:795465/1‑140 (MQ=255)

                                                          tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTa                                                                                         >  1:9825137/1‑129 (MQ=255)

                                                          tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGc                                                                              >  1:3463816/1‑140 (MQ=255)

                                                            gTCGTTGAATCTGGCGACAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCtt                                                                            <  2:11506887/140‑1 (MQ=255)

                                                               gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAg                                                                         >  1:4782210/1‑140 (MQ=255)

                                                               gTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGGTGTTATTGCGGGTCGCGCGTTGCAGTGG‑G‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACAGCAGGTTTATGTTGAAAGCTTCAg                                                                         >  2:3857368/1‑140 (MQ=255)

                                                                ttGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGc                                                                        <  2:5695747/140‑1 (MQ=255)

                                                                 tGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                            >  2:890999/1‑119 (MQ=255)

                                                                 tGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                            <  1:890999/119‑1 (MQ=255)

                                                                       tGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAAc                                                                 <  2:2143416/140‑1 (MQ=255)

                                                                       tGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAAc                                                                 >  1:5940223/1‑140 (MQ=255)

                                                                        ggCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACa                                                                >  1:5133453/1‑140 (MQ=255)

                                                                         gCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAg                                                               >  1:6007272/1‑140 (MQ=255)

                                                                          cGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg                                                              <  1:7065559/140‑1 (MQ=255)

                                                                          cGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑GTG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg                                                              <  2:4365597/140‑1 (MQ=255)

                                                                               aTGCCGCACTCTGCCTGCTGGCACAACCCGGCGTGGTTATTGCGGGTCGCGCGAGGCAGT‑TGG‑G‑CGACGCCATTAAACTCATGATCGACAGGCTGAAACCGCAGGTTCATGGTGACGGCTTCAGCGTCGCACAGGCCGtg                                                         >  2:69376/1‑139 (MQ=255)

                                                                                        tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGcc                                                <  1:7362062/140‑1 (MQ=255)

                                                                                        tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGcc                                                >  2:6085712/1‑140 (MQ=255)

                                                                                             ctgctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGTCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACtt                                           <  1:10947686/140‑1 (MQ=255)

                                                                                             ctgctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACtt                                           <  2:3254980/140‑1 (MQ=255)

                                                                                               gctgGCACAACCCTGCGTTGTTTTTGAGCGTCGCGCGATGAAGT‑TGG‑G‑CGACGGCATTAAATTCATGAACGACAGGCTGAAACCGCAGTTTAATGTGGACAGCGTCAGCCTCGAACTGGCTGTCTTTCGCGCGAAcgtta                                         >  2:9526792/1‑136 (MQ=255)

                                                                                               gctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTaa                                         >  2:1666962/1‑140 (MQ=255)

                                                                                                ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                        >  2:4813535/1‑140 (MQ=255)

                                                                                                ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                        >  2:3496892/1‑140 (MQ=255)

                                                                                                  ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                      <  2:1489738/140‑1 (MQ=255)

                                                                                                  ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                      >  2:2926596/1‑140 (MQ=255)

                                                                                                  ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAAAGACAGGCTGATCCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                      >  2:7469462/1‑140 (MQ=255)

                                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    <  1:3823118/140‑1 (MQ=255)

                                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    <  2:8742281/140‑1 (MQ=255)

                                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    <  2:3133104/140‑1 (MQ=255)

                                                                                                    cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  2:6806845/1‑140 (MQ=255)

                                                                                                      caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  2:2017695/140‑1 (MQ=255)

                                                                                                      caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  2:4074950/140‑1 (MQ=255)

                                                                                                      caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  1:8804320/140‑1 (MQ=255)

                                                                                                      caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  2:8519912/140‑1 (MQ=255)

                                                                                                        aCCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAATTCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCt                                >  1:2726828/1‑140 (MQ=255)

                                                                                                         cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               >  2:2781962/1‑140 (MQ=255)

                                                                                                         cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               >  1:7598143/1‑140 (MQ=255)

                                                                                                         cccTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:305468/140‑1 (MQ=255)

                                                                                                            tGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCgg                            <  2:553104/140‑1 (MQ=255)

                                                                                                              cGTTGTTATTGCGGGTCGCGAGATGCAGT‑T‑G‑GTCGACGCCATTAAAATCATGTACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCAAACTTAAGTGCAGTATTCGGTa                          >  1:1711581/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:10000064/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:6555503/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:8983674/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:2412843/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  1:6645933/1‑140 (MQ=255)

                                                                                                                   ttATTGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  1:4711716/1‑140 (MQ=255)

                                                                                                                      ttGCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                  <  2:2341840/140‑1 (MQ=255)

                                                                                                                        gCGGGTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTCATGTTGACAGCTTCAGCCTCGAACAGGCAGGCTAACGCGCCAACTTAAGTGCAGTCTGCGGTACACAGCTACa                >  1:3977034/1‑140 (MQ=255)

                                                                                                                        gCGGGTCGCGCGAGGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGTCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACACGCAGTCTAAAGCGCCAACTTAAGTGCAGTCTGCGGTACACAGCTACa                >  2:5170329/1‑140 (MQ=255)

                                                                                                                           ggTCGCGCGATGCAGT‑TGG‑G‑CGACGCTATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTTCACAGCTACAGCa             <  1:6263053/140‑1 (MQ=255)

                                                                                                                           ggTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             <  2:1912372/140‑1 (MQ=255)

                                                                                                                           ggTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             <  1:7987131/140‑1 (MQ=255)

                                                                                                                            gTCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaa            <  1:6431796/140‑1 (MQ=255)

                                                                                                                             tCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           >  1:2624161/1‑140 (MQ=255)

                                                                                                                             tCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           <  1:4449578/140‑1 (MQ=255)

                                                                                                                             tCGCGCGATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           >  2:775559/1‑140 (MQ=255)

                                                                                                                                cgcgATGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAAAGATTCAGCCTCTAACAGTCAGTCTAACGCGCCACCTAAAGAGCATTCTAAGGTACACAGATACAGCAAAGaa        >  2:9803421/1‑140 (MQ=255)

                                                                                                                                cgcgATGCAGT‑TGG‑G‑AGACGCCATTAAAATCATGAACGCCAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa        <  2:4508730/140‑1 (MQ=255)

                                                                                                                                     tGCAGT‑TGG‑G‑CGACGCCATTCAAATCATGAACGCCAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCCGTCTTCGGTACACAGCGACAGCAAAGAATGGTg   >  2:4689425/1‑140 (MQ=255)

                                                                                                                                     tGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAATGAATGGTg   >  2:10443046/1‑140 (MQ=255)

                                                                                                                                     tGCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg   <  1:11594237/140‑1 (MQ=255)

                                                                                                                                      gCAGT‑TGG‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGc  <  2:3730952/140‑1 (MQ=255)

ATGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGT‑T‑G‑G‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NZ_CP025268/1013379‑1013651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`