BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,690,360	(C)_5→6	pseudogene (1047/1403 nt)	fumC ←	class II fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,690,355	1	.	C	98.4%	2554.4 / 27.0	744	pseudogene (1052/1403 nt)	fumC	class II fumarate hydratase
Reads supporting (aligned to +/- strand): ref base . (8/4); new base C (324/406); total (332/412)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 810 total aligned reads displayed.

TTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NZ_CP025268/1690216‑1690489

    cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑ccc                                                                                                                                    <  2:5389912/140‑1 (MQ=255)

      cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑ccccc                                                                                                                                  <  1:2247449/140‑2 (MQ=255)

          aTTCGGTTCAATACCCACTGCGCAGTGTTTGTTTAAACTTTCCATGCCAGCTGCCAGCAAGCGCACCGATTGCTGGAAATTGTGGATCTCGATTGGCCGGAAGACGGTCAGGGCACAGTTAGCGGTCGC‑G‑c‑cccccgtgg                                                                                                                              >  2:8242893/1‑135 (MQ=255)

               gTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATc                                                                                                                          >  2:11586008/1‑140 (MQ=255)

               gTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATc                                                                                                                          >  1:3111370/1‑140 (MQ=255)

                 tCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGc                                                                                                                        <  2:6518046/140‑1 (MQ=255)

                 tCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGc                                                                                                                        <  2:9398292/140‑1 (MQ=255)

                   aaTACCCACTGCGCAGTGTTTGTTAAAACTATCCCTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CGCCCCATGATGATCGccc                                                                                                                      >  2:11294704/1‑139 (MQ=255)

                     tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCAcg                                                                                                                    <  1:3424673/140‑1 (MQ=255)

                         cACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCAcgtcgt                                                                                                                >  1:929886/1‑140 (MQ=255)

                          aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGtt                                                                                                               >  2:6737480/1‑140 (MQ=255)

                          aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGtt                                                                                                               >  1:9139581/1‑140 (MQ=255)

                            tGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCTC‑C‑CCCCATGTTGATCGCCACGTCGTTcc                                                                                                             >  1:6267778/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAATCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGTAAGACGTTCAGTACAAATTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            <  2:8849888/140‑1 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAACTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:8269282/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:2612036/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:5218519/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  1:4994930/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  2:2270802/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            <  2:5728391/140‑1 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTccc                                                                                                            >  2:3457621/1‑140 (MQ=255)

                             gcgcAGTGTTTGTTAAAACTTTCCATGCCAGCTGCCAGCACGCGCACCGATGGCCGGAAATTGTGGCTCACCAGTGGACGGAAGACGTTCAGTTCAATGTTACGGGATGC‑GCC‑CCCCATGGTGATCGCCACGTCGTTccc                                                                                                            >  2:7612611/1‑140 (MQ=255)

                                ccgtgtTTGTTAAACCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGACGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCAt                                                                                                         <  2:2053250/138‑1 (MQ=255)

                                   tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCAc                                                                                                      >  1:7483330/1‑140 (MQ=255)

                                   tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCAc                                                                                                      >  1:11434529/1‑140 (MQ=255)

                                     tttgttACACCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCAccg                                                                                                    <  2:1471443/140‑2 (MQ=255)

                                     tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                                    >  1:6447881/1‑140 (MQ=255)

                                      ttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTg                                                                                                   >  1:2772607/1‑140 (MQ=255)

                                       tgttAAAACTTTCCATGCCATCTGCCAGCAAGCTCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCAGCACCTGa                                                                                                  <  2:8115264/140‑1 (MQ=255)

                                       tgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                                  <  2:4012429/140‑1 (MQ=255)

                                        gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGTCGACGTTCAGTTCAAAGTTAGCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAc                                                                                                 >  2:10006033/1‑140 (MQ=255)

                                          tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  1:200428/140‑1 (MQ=255)

                                             aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGTAAGC‑GCC‑CCCCATGTTGATCTCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  2:10709362/1‑140 (MQ=255)

                                             aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  1:11260724/1‑140 (MQ=255)

                                             aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  1:9649204/1‑140 (MQ=255)

                                               cTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag                                                                                          <  1:3250413/140‑1 (MQ=255)

                                                tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  2:1885105/140‑1 (MQ=255)

                                                tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  2:550590/140‑1 (MQ=255)

                                                tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:7704237/140‑1 (MQ=255)

                                                gttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:8963182/139‑1 (MQ=255)

                                                 ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                        >  1:10027008/1‑140 (MQ=255)

                                                 ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                        <  1:3772419/140‑1 (MQ=255)

                                                 ttCCATGCCATATGACAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGAAGGAAGACGTTCTGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                        >  2:10271969/1‑140 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:2940370/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:10402699/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:1730131/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:7232016/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:6881246/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:917885/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:10768956/140‑1 (MQ=255)

                                                       gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAAGTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:10229270/140‑1 (MQ=255)

                                                         cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                <  2:4994929/140‑1 (MQ=255)

                                                          aTCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATg                                                                               >  2:6268137/1‑140 (MQ=255)

                                                          aTCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATg                                                                               <  1:8142823/140‑1 (MQ=255)

                                                            cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCACAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                             <  2:4731779/140‑1 (MQ=255)

                                                            cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                             <  2:58702/140‑1 (MQ=255)

                                                            cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                             <  2:11090126/140‑1 (MQ=255)

                                                                cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                         <  2:6394011/140‑1 (MQ=255)

                                                                cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                         <  1:2420335/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:4545318/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:8457469/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  2:3312297/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:7856330/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:6891352/140‑1 (MQ=255)

                                                                 aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:1152528/140‑1 (MQ=255)

                                                                     aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑ccc                                                                                                                                    <  1:8691165/75‑1 (MQ=255)

                                                                     aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑ccc                                                                                                                                    >  2:8691164/1‑75 (MQ=255)

                                                                       cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                  >  2:4340411/1‑140 (MQ=255)

                                                                       cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                  >  1:10100680/1‑140 (MQ=255)

                                                                        gcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGtt                                                                 <  1:3970556/140‑1 (MQ=255)

                                                                              aTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTc                                                           >  2:2865378/1‑140 (MQ=255)

                                                                              aTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTc                                                           >  1:9762359/1‑140 (MQ=255)

                                                                                    ggAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCTCTTTc                                                     >  2:2615121/1‑140 (MQ=255)

                                                                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCCTGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                <  1:11081190/140‑1 (MQ=255)

                                                                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                <  2:3116369/140‑1 (MQ=255)

                                                                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                <  1:7286304/140‑1 (MQ=255)

                                                                                         ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                <  1:712380/140‑1 (MQ=255)

                                                                                            tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCACCATCACCTGACAGCAGAGCAGGGTTATTGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCag                                             >  2:9060948/1‑139 (MQ=255)

                                                                                             ggATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCCTGGTTAATGCCTCACACTGTGTTGGGTGCACTTTCCCCGGCATg                                            >  2:4545337/1‑140 (MQ=255)

                                                                                             ggATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTGTCCCCGGCATg                                            <  2:10124852/140‑1 (MQ=255)

                                                                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                   >  1:4652740/1‑140 (MQ=255)

                                                                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                   >  1:8803704/1‑140 (MQ=255)

                                                                                                      ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                   >  1:5637066/1‑140 (MQ=255)

                                                                                                         gACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                                <  1:4047388/140‑1 (MQ=255)

                                                                                                             gAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCt                            >  1:538332/1‑140 (MQ=255)

                                                                                                              aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:3377732/1‑140 (MQ=255)

                                                                                                              aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:6070121/1‑140 (MQ=255)

                                                                                                              aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:10527354/1‑140 (MQ=255)

                                                                                                              aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGGTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:5868182/1‑140 (MQ=255)

                                                                                                               aGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCa                          <  2:5141026/140‑1 (MQ=255)

                                                                                                               aGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCa                          <  1:1349649/140‑1 (MQ=255)

                                                                                                                gACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAt                         >  2:7386180/1‑140 (MQ=255)

                                                                                                                     tcagttcaAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTcc                    <  2:7857567/140‑1 (MQ=255)

                                                                                                                        gttcaAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCggg                 >  1:6665317/1‑140 (MQ=255)

                                                                                                                          tcaAAGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAt               <  1:6050840/140‑1 (MQ=255)

                                                                                                                              aGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag           >  1:9488337/1‑140 (MQ=255)

                                                                                                                              aGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag           >  2:8456599/1‑140 (MQ=255)

                                                                                                                              aGTTACCGGAAGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag           >  1:4343457/1‑140 (MQ=255)

                                                                                                                                       aaGC‑GCC‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCa  <  1:702914/140‑1 (MQ=255)

                                                                                                                                       aaGC‑G‑CACCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCATCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAGTTCACCa  >  2:977266/1‑140 (MQ=255)

TTCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑CCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NZ_CP025268/1690216‑1690489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`