BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	95.5%	2085.2 / 74.8	580	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (13/13); new base T (331/220); total (345/235)
Fisher's exact test for biased strand distribution p-value = 3.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 647 total aligned reads displayed.

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

    gACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tt                                                                                                                                   <  1:10342668/140‑1 (MQ=255)

     aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttt                                                                                                                                  >  1:5425644/1‑140 (MQ=255)

       aGCGACCCGTGGGCCTGAGAAGCGGCAACCCAGGCGTCGCATACAGTTGGCAACTGTAGGACGGGGGGCATATGACCGGGTGTATTGACCGGCGTCTGAACGGCAACAACAAACGCAGGGTGACTCGCCAGCG‑tt‑tttta                                                                                                                                <  2:1971471/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  1:3448737/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  1:8101536/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  1:7815000/140‑3 (MQ=255)

       aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑tttta                                                                                                                                <  1:4562208/140‑3 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              >  2:5197620/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  2:4698504/140‑5 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              >  1:2730424/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              >  1:5178548/1‑136 (MQ=255)

         cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑tt‑ttttacg                                                                                                                              <  2:5368041/140‑5 (MQ=255)

                tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTtat                                                                                                                        >  2:5859069/1‑140 (MQ=255)

                 gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTgata                                                                                                                       >  1:10956156/1‑140 (MQ=255)

                     cTGAGAAGCGGCAACACCGGCGTAGCATCCAGTTGGCAACTGTAGTACGGGGGGCATATGACCTGGATTATTGACCGGCGTCTGAACGGCAAAAACAACAGCCTGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   <  2:4453303/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   <  2:9005446/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   <  2:3746024/140‑1 (MQ=255)

                     cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgc                                                                                                                   <  2:8017126/140‑1 (MQ=255)

                       gagaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAgcgc                                                                                                                 <  1:5973158/140‑1 (MQ=255)

                          aaGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCaaa                                                                                                              <  1:11065641/140‑1 (MQ=255)

                             cGGCAACACAGGCGTAGCATACAGTTGGCAAATGAAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:10821244/1‑140 (MQ=255)

                               gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  1:9096581/140‑1 (MQ=255)

                                cAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAAc                                                                                                        <  1:4047147/140‑1 (MQ=255)

                                    acaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTc                                                                                                    <  2:2256908/140‑1 (MQ=255)

                                     caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:2486212/140‑1 (MQ=255)

                                        gCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGAtc                                                                                                >  2:4152549/1‑140 (MQ=255)

                                          gTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGAtctc                                                                                              >  1:7010592/1‑140 (MQ=255)

                                           tAGCATACAGTGGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  2:11299273/140‑1 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCACATTAAACAGTCGATCTCCGa                                                                                           >  2:1030057/1‑140 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           >  1:1900034/1‑140 (MQ=255)

                                             gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  1:6675283/140‑1 (MQ=255)

                                              cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  1:7421557/1‑140 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  2:491125/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1827273/140‑1 (MQ=255)

                                               aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:11605936/140‑1 (MQ=255)

                                                tACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGc                                                                                        >  1:2199118/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATACAGCGCAAATTTAACAGTCGATCTCCGAGGCCa                                                                                      >  2:8624281/1‑140 (MQ=255)

                                                  cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:6303134/1‑140 (MQ=255)

                                                   aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTCTAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCag                                                                                     >  2:6975813/1‑139 (MQ=255)

                                                   aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                     >  1:6570487/1‑140 (MQ=255)

                                                   aGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGGTATAATGCGCAAATTAAACAGTCGATCTCCGAGGCCAt                                                                                     >  2:11554437/1‑140 (MQ=255)

                                                    gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                    >  1:2637665/1‑140 (MQ=255)

                                                     ttGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATta                                                                                   >  1:4652818/1‑140 (MQ=255)

                                                      tGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaa                                                                                  <  1:9224217/140‑1 (MQ=255)

                                                       ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAATGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:7702558/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  2:1630856/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:10014176/1‑140 (MQ=255)

                                                        gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:3265016/1‑140 (MQ=255)

                                                             tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  1:11356308/1‑140 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      <  2:3597538/140‑1 (MQ=255)

                                                                  taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                      >  1:5522070/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:9596694/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  1:7246702/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:1238998/1‑140 (MQ=255)

                                                                       ggggCATATGACCTGGTTTATTGACCGGCGTCTGAAAGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:2676196/1‑140 (MQ=255)

                                                                        gggCATATGACCTGGTTTATTGACCGGCGTCTGATCGGCAAAATCAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactga                                                                >  2:5721652/1‑140 (MQ=255)

                                                                         ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:2727429/1‑140 (MQ=255)

                                                                               tGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGACTCGCCAGCGTTT‑TTTACGCTGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                         <  2:3389650/140‑1 (MQ=255)

                                                                                 aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       <  1:8232732/140‑1 (MQ=255)

                                                                                 aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       >  1:9684695/1‑140 (MQ=255)

                                                                                 aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       <  1:6232933/140‑1 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAGAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:676644/1‑140 (MQ=255)

                                                                                     ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  1:8691536/1‑140 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCTATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:4883220/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:7637477/140‑1 (MQ=255)

                                                                                      gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:951202/140‑1 (MQ=255)

                                                                                        ttATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGaa                                                >  2:10485260/1‑140 (MQ=255)

                                                                                         tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               <  2:822077/140‑1 (MQ=255)

                                                                                         tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               <  2:9256169/140‑1 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:10768422/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             <  1:1630856/140‑1 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:3186847/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  2:4245174/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  2:11490414/1‑140 (MQ=255)

                                                                                           ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATGAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:99008/1‑140 (MQ=255)

                                                                                              aCCGGCGTCTGAACGGCAAAAACAAAAGCATGGAGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAAAAATAACCGTTCGGTGACTGACGTCGACAGCGCCGAATCCGTa                                          <  2:2965041/140‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATACAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:782516/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:3871294/91‑1 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  1:6851871/1‑140 (MQ=255)

                                                                                                cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        >  2:2405129/1‑140 (MQ=255)

                                                                                                 ggCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATcc                                       >  1:11469970/1‑140 (MQ=255)

                                                                                                   cGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                     >  2:8490125/1‑140 (MQ=255)

                                                                                                     tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                   >  2:11074601/1‑140 (MQ=255)

                                                                                                        gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:1413652/140‑1 (MQ=255)

                                                                                                         aaCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCAc                               >  1:2919099/1‑140 (MQ=255)

                                                                                                          aCGGCACACACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATATAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  1:8566628/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  1:2048561/140‑1 (MQ=255)

                                                                                                          aCGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                              <  2:7403615/140‑1 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  2:6017795/1‑140 (MQ=255)

                                                                                                             gCAAAAACAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  1:3757387/1‑140 (MQ=255)

                                                                                                               aaaaaCAACAGCCAGGTGACTCGCCACCG‑TTATTTACGCCGTTATACCGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGGGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                         <  2:9694730/140‑1 (MQ=255)

                                                                                                               aaaaaCAAAAGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATCCCCACGGAAGa                         <  2:6495222/140‑1 (MQ=255)

                                                                                                                     aaaaGCATGGTGAATCGCCAGCG‑TTATTTACGCCGGTAGAACGCGCAAATTATACAGTCGATCTCCGAGGCCATTAATACGCGTTCGGTGACTGACGTCGACAGCGGCCAATCCGTATCCATTCCCACGGACGAACTTAg                   >  2:7285208/1‑140 (MQ=255)

                                                                                                                        aGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                >  1:10510501/1‑124 (MQ=255)

                                                                                                                        aGCATGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGa                >  1:4251367/1‑140 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGGGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             <  2:3187775/140‑1 (MQ=255)

                                                                                                                             ggTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa           >  1:3590038/1‑140 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  1:10216797/140‑1 (MQ=255)

                                                                                                                                      ccAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGACACCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc  >  2:3964161/1‑140 (MQ=255)

                                                                                                                                      ccAGCGTTT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcatc  <  1:8897888/140‑1 (MQ=255)

CAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCG‑TT‑TTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873692‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`