BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,204,490	(A)_7→8	pseudogene (175/3632 nt)	CXP41_RS11705 →	DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,204,483	1	.	A	93.9%	2011.3 / 104.6	560	pseudogene (168/3632 nt)	CXP41_RS11705	DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base . (15/19); new base A (254/270); total (270/290)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 600 total aligned reads displayed.

ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204344‑2204617

ctAACGCCCAACTGGAACTGAAATATAAACAAGGCAACACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGagaag                                                                                                                                             <  1:1459656/139‑1 (MQ=255)

  aaCGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGTATTGCTACAAAAAGCTGAgaag‑a‑a                                                                                                                                         >  2:9143038/1‑140 (MQ=255)

      cccAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑aa                                                                                                                                    >  1:6474774/1‑140 (MQ=255)

         aaCTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑a‑aaa‑aa‑aac                                                                                                                                >  2:223212/1‑138 (MQ=255)

              gAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGt                                                                                                                           >  1:324897/1‑140 (MQ=255)

                          aaaaaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATga                                                                                                               <  2:9833725/140‑1 (MQ=255)

                          aaaaaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATga                                                                                                               >  1:2783358/1‑140 (MQ=255)

                             aaaGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGaa                                                                                                            <  2:5245742/140‑1 (MQ=255)

                               aGGCAAAACTCCGCACAGTCATCGTCGCTGGCCGGGCGATCCAGTGTCCTTTATCACTGGAAGTCTCATCCAGACATTGGGTGATTAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑AA‑ATCATTGTCTGGCGTTATGAGAAtt                                                                                                          <  2:8126475/140‑1 (MQ=255)

                                 gCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAAtttt                                                                                                        >  1:8084630/1‑140 (MQ=255)

                                     aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGATCCAGTGTCAGTTATCACTGGAAGTCTCATCCAGACATTGTGTGATGAATTGCTACAAAAAGCTGATAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  1:11072425/140‑1 (MQ=255)

                                     aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCAc                                                                                                    <  1:4984314/140‑1 (MQ=255)

                                     aaCGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGAGTTATGAGAATTTTTCAc                                                                                                    <  1:1318355/140‑1 (MQ=255)

                                         ccgcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGa                                                                                                <  2:7769949/140‑1 (MQ=255)

                                           gcTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGt                                                                                              <  2:1572854/140‑1 (MQ=255)

                                                 tCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGt                                                                                        >  1:1002386/1‑140 (MQ=255)

                                                  cATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTc                                                                                       <  1:10529932/140‑1 (MQ=255)

                                                  cATCGTCGCTGGCCGGGAGAACCAGTGTCAGTTATCACAGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATTAGAATTTTTCACTGGAGTGGCAGTc                                                                                       <  2:3006796/140‑1 (MQ=255)

                                                       tcgCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCa                                                                                  >  2:4285452/1‑140 (MQ=255)

                                                         gCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACAGTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATc                                                                                >  2:6599899/1‑140 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:1845027/1‑140 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  2:451289/1‑140 (MQ=255)

                                                           tGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCAc                                                                              >  1:4873713/1‑140 (MQ=255)

                                                            ggCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACg                                                                             <  1:5658638/140‑1 (MQ=255)

                                                             gCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGc                                                                            <  2:9653196/140‑1 (MQ=255)

                                                               cGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAg                                                                          >  2:9532175/1‑140 (MQ=255)

                                                                 ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGACGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGc                                                                        >  1:5717444/1‑140 (MQ=255)

                                                                 ggCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACAGTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGc                                                                        >  1:11416968/1‑140 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  1:604731/1‑140 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  2:5732091/1‑140 (MQ=255)

                                                                  gCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGcc                                                                       >  2:6099127/1‑140 (MQ=255)

                                                                       ccAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCaa                                                                  >  1:8599898/1‑140 (MQ=255)

                                                                          gtgtCCGTTATCACTGGCAGTCTCATCCAGCCATTGGGTGAGGAATTGCTACAAAAAGCTGAGAAG‑ACAAA‑AA‑A‑CATTGTCGGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACtt                                                               <  2:6295462/140‑1 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:3490890/1‑140 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:2541547/1‑140 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  2:10576878/1‑140 (MQ=255)

                                                                                gTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCgg                                                         >  1:11583437/1‑140 (MQ=255)

                                                                                  taccACTGCACGTCTCCGCCAGACCTTGGGGGATGAATTGCTACCAAAAGCCCAGAAC‑A‑AAACCA‑C‑CCGTGTCTCGCGTTAAGAGAAATTTTCACTGGAGTGGCAGTCCGCCATCACGCCGGCCATCACCTAGATCGGCg                                                       <  2:7912076/137‑1 (MQ=255)

                                                                                  tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       >  1:4365171/1‑140 (MQ=255)

                                                                                  tATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCg                                                       <  1:7499123/140‑1 (MQ=255)

                                                                                    tCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGaa                                                     <  1:3661949/140‑1 (MQ=255)

                                                                                     cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCCTCAACTTGATCGGCGAac                                                    >  2:1398798/1‑140 (MQ=255)

                                                                                     cACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAac                                                    >  2:2338759/1‑140 (MQ=255)

                                                                                       cTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCCTCAACTTGATCGGCGAacac                                                  >  1:8332257/1‑140 (MQ=255)

                                                                                         ggAAGTCTCTTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGATTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                <  2:4635563/140‑1 (MQ=255)

                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                <  2:4800554/140‑1 (MQ=255)

                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                <  2:5151431/140‑1 (MQ=255)

                                                                                         ggAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaa                                                >  2:10218785/1‑140 (MQ=255)

                                                                                          gAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaaa                                               >  1:7738983/1‑140 (MQ=255)

                                                                                          gAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACaaa                                               <  1:5274930/140‑1 (MQ=255)

                                                                                            aGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAcc                                             >  2:5440885/1‑140 (MQ=255)

                                                                                            aGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAcc                                             >  2:1848958/1‑140 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           >  2:10999846/1‑140 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑AATA‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACAGAAACCCt                                           >  2:9330701/1‑140 (MQ=255)

                                                                                              tctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑AATA‑CAGTGTCGGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCt                                           <  2:10825955/140‑1 (MQ=255)

                                                                                               ctcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTc                                          <  1:5862903/140‑1 (MQ=255)

                                                                                                tcATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCa                                         >  1:3345449/1‑140 (MQ=255)

                                                                                                  aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAAt                                       >  1:10145242/1‑140 (MQ=255)

                                                                                                  aTCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGAGCGGCGAACACAAACCCTCAAt                                       >  2:8424548/1‑140 (MQ=255)

                                                                                                   accAGACAGTGGGGGATGCAGTGCTACAAAAAGCAGAGAAGCA‑AAC‑CA‑C‑CCTGGTCTGGCGTTATGAGACTTTTTCACTGGAGTGGCAGTCCGCCCTCACGCAGGCCATCAACTTGCTCGGCGAACACACACCCTCaagc                                      <  2:1139126/139‑3 (MQ=255)

                                                                                                    ccAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcc                                     >  1:4066854/1‑140 (MQ=255)

                                                                                                      aGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccg                                   >  1:2904851/1‑140 (MQ=255)

                                                                                                      aGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccg                                   <  2:8648647/140‑1 (MQ=255)

                                                                                                        aCATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 >  1:3853239/1‑140 (MQ=255)

                                                                                                        aCATTGGGTGATGAAGTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggc                                 <  2:11130326/140‑1 (MQ=255)

                                                                                                         cATTGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcc                                <  1:2415641/140‑1 (MQ=255)

                                                                                                           ttGGGTTATGAATTTCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑AATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccg                              <  2:7286837/140‑1 (MQ=255)

                                                                                                           ttGGGTGATGAATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATcccggcccg                              <  1:4683145/140‑1 (MQ=255)

                                                                                                              ggTGATGAATTGCTACAAAAAGCTGAGTAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTAACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAAACCTCAATCACGGCCCGGAc                           >  2:11610372/1‑140 (MQ=255)

                                                                                                                  atgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACCATg                       >  1:8254897/1‑140 (MQ=255)

                                                                                                                   tgaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATgg                      <  2:8560689/140‑1 (MQ=255)

                                                                                                                    gaATTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggc                     >  1:258884/1‑140 (MQ=255)

                                                                                                                      aTTGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATggcgg                   >  2:203102/1‑140 (MQ=255)

                                                                                                                        tGCTACATCTAGCTGAGAAG‑ACCAA‑AA‑A‑CATTGTCTGGCTTGATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCTTCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:7040105/140‑1 (MQ=255)

                                                                                                                        tGCTACAAACAGCTGAGAAGAA‑AAA‑AA‑A‑CTTTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:6383564/140‑1 (MQ=255)

                                                                                                                        tGCTACAAACAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:546794/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:6916896/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:1641879/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:11236740/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:10264620/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:4549053/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:1070903/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:1002386/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:9691627/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:3287475/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:8767343/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:3697680/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:7247525/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  1:6706068/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGgcggcg                 <  2:5949975/140‑1 (MQ=255)

                                                                                                                        tGCTACAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACGGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCACTCCCGGCCCGGACAATGgcggcg                 <  1:901167/140‑1 (MQ=255)

                                                                                                                             cAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc            >  2:8917081/1‑140 (MQ=255)

                                                                                                                             cAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc            <  1:1962039/140‑1 (MQ=255)

                                                                                                                             cAAAAAGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGc            <  1:10839042/140‑1 (MQ=255)

                                                                                                                               aaaaGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCt          >  2:2671757/1‑140 (MQ=255)

                                                                                                                                 aaGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGt        >  1:939872/1‑140 (MQ=255)

                                                                                                                                  aGCTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCAGCAACTTGATCGGCGACCACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTa       >  2:7487977/1‑140 (MQ=255)

                                                                                                                                   gCTGAGAAGAA‑AAA‑AA‑A‑CATGGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTAt      <  1:6868277/140‑1 (MQ=255)

                                                                                                                                    cTGAGAAGAA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATc     >  1:92673/1‑140 (MQ=255)

                                                                                                                                       agaagaA‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATcgcg  <  1:2217301/140‑1 (MQ=255)

ATAACGCCCAACTGGAACTGAAATATAAAAAAGGCAAAACGCCGCTCAGTCATCGTCGCTGGCCGGGCGAACCAGTGTCCGTTATCACTGGAAGTCTCATCCAGACATTGGGTGATGAATTGCTACAAAAAGCTGAGAAG‑A‑AAA‑AA‑A‑CATTGTCTGGCGTTATGAGAATTTTTCACTGGAGTGGCAGTCCGCCATCACGCAGGCCATCAACTTGATCGGCGAACACAAACCCTCAATCCCGGCCCGGACAATGGCGGCGCTAGCCTGTATCGCG  >  NZ_CP025268/2204344‑2204617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`