BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	95.9%	1877.8 / 65.4	568	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (10/13); new base C (213/328); total (225/343)
Fisher's exact test for biased strand distribution p-value = 8.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 605 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑CCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTC  >  NZ_CP025268/2292451‑2292725

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                           >  2:4727887/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                           >  1:4924369/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                           >  2:6819424/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                           >  1:7966678/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                           >  1:9599455/1‑140 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑ccccc                                                                                                                                     <  1:2306379/140‑1 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGAGGTAGTTTTAATAGCACCGCCTGCTTACTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCGTGTCGATAGCGACAAAGAC‑GG‑ccccc                                                                                                                                     >  2:11559751/1‑140 (MQ=255)

         gTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑cccccccat                                                                                                                                 <  2:4005454/140‑4 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGG‑CCCCCCA‑Tc                                                                                                                                >  1:8324519/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑cccccccatc                                                                                                                                >  1:5274726/1‑136 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑cccccccatc                                                                                                                                >  1:10024247/1‑136 (MQ=255)

             gTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCttt                                                                                                                             <  1:8772787/140‑1 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAAc                                                                                                                       <  2:10827442/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGcgcc                                                                                                            <  1:8848343/140‑1 (MQ=255)

                              ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGcgcc                                                                                                            <  1:432570/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTCCTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATACCCCCTCTTTAACCTACAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGcgccc                                                                                                           <  2:7857225/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGcgccc                                                                                                           <  1:9028891/140‑1 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCACCCTCTTTAACCTCCCGTAGTCCTCCCTTTTCGATAGCGACCAAGAC‑GGCCCCCCCA‑TCTTTGTCAACGAGGAAGcgcccggc                                                                                                        >  2:11120391/1‑138 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACACAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGcgcccgccgt                                                                                                      >  2:8553760/1‑138 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                      >  1:703951/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                      >  1:4512226/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                     >  2:9375533/1‑140 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTACTCg                                                                                                <  2:7356612/140‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                                <  2:2596002/140‑1 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                                <  2:5559947/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                              <  2:6123972/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                              <  1:11061534/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                              <  2:7491244/140‑1 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                         >  1:3631750/1‑140 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                         >  1:8418897/1‑140 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                     >  2:4394316/1‑140 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTAAACCATTTAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                     >  2:5317931/1‑140 (MQ=255)

                                                      aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                                                                    >  1:6645385/1‑140 (MQ=255)

                                                      aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCg                                                                                    >  1:9352856/1‑140 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTCACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTATTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                                 <  2:3804166/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCATTTCGATAGCGACAAAGAC‑GGCCCCCCCC‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                                 <  2:1422613/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCACATCCCCCCTCGTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGACCCCCCA‑TCTTTTTCAACGATTAATCGCCCGCCTGTAATCGTGGTATCATTCGCTgc                                                                                >  2:3129966/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                                >  1:5963371/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                            <  1:11006029/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                            <  2:9419212/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                            <  2:9114981/140‑1 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑ACTTTTTCAACGATTAAGCGCCCGCCATTAATCGTGGTATCATTCGCTGCGCCa                                                                            <  1:7578184/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCTGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                           <  1:2491676/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                           <  1:2900890/140‑1 (MQ=255)

                                                               tttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt                                                                           <  2:3394672/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                         <  1:7201412/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTGTTTCAACGATTAAGCGCCCGCCTTTAAGCGTGGTATCATTCGCTGCGCCATaa                                                                         >  2:9031871/1‑140 (MQ=255)

                                                                   tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                       <  1:6819425/140‑1 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                  >  2:4185520/1‑140 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                  >  2:8748359/1‑140 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                  >  2:274062/1‑140 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCCGTAGTCCTCCCTTTTCGATAGCGCCAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                 <  1:1237829/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                 <  1:7446173/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                                 <  1:10795495/140‑1 (MQ=255)

                                                                           ctcaaatgcaaatCCCCCCTCTTTAACCTCCATTAGTCCTCCCTTTTCGAGAGCGCCAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                               <  2:5079519/138‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                               <  1:11515166/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                               <  2:9599454/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                               <  1:6107225/140‑1 (MQ=255)

                                                                           cGCAAAGGCAAATCCCCCCTCGTTACCCCCCACTACTCCGCCCGGATCGATAGCGCCAAAGAC‑GGCCCCCCCA‑GCAGTTTCCACGATTAAGCGCCCGCCTTTAATCGTGGTCTCATTCGCTGCGCCATACACCTGTAGca                                                               <  2:8399885/140‑1 (MQ=255)

                                                                             caaatgcaactCCCCCCTCTTTACCCTCCAGTAGTCCTCCCTTTGCGATAGCGACAAAGAC‑GG‑CCCCCCACTCTTTTTCAACGATTAAGCGCCCGCCATTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                             <  2:6489325/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                             <  2:10092982/140‑1 (MQ=255)

                                                                               aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc                                                           >  1:9714186/1‑140 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                          <  2:2130600/140‑1 (MQ=255)

                                                                                    aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                      <  1:10253310/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                   <  2:6643049/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                   <  2:4512225/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                                <  1:1737733/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCAGTAGCACACCATTATTGAGCg                                                <  2:9269698/140‑1 (MQ=255)

                                                                                           cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt                                               >  1:7368449/1‑140 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                               >  1:3741491/1‑123 (MQ=255)

                                                                                              tctTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCCTTATTGAGCGTAGt                                            >  2:1329308/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                  >  2:1037441/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                  >  2:6253518/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                                 <  2:9714209/140‑1 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                                 >  1:3120054/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAATAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                                >  2:8857313/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTCGTCTGGTTTGCcgc                                >  2:2422077/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCagc                                >  2:10226974/1‑140 (MQ=255)

                                                                                                              gtCCTCCCTTTCCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCATTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                            <  1:10535513/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTGTCGCTAGCGACCACGAC‑GGCCCCCCCA‑GCTTTTTCAACGATTAAGCGCCCGCCTTTCATCGTGGTCTCCGTCGCTGCGCCATAAACCTGTAGCACACCATGATTGAGCGTCGTCTGGTTTGCCGCGCCa                            <  2:714307/140‑1 (MQ=255)

                                                                                                                cctccCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc                          >  1:415120/1‑140 (MQ=255)

                                                                                                                cctccCTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAcc                          >  1:473995/1‑140 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                       <  1:3386268/140‑1 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                    >  2:5919918/1‑140 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCAGTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                    >  1:3965119/1‑140 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                  <  2:7769676/140‑1 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                  <  2:2932992/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc                 <  1:9118689/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc                 <  2:11368058/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCANAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCNATTTGAAc                 <  1:6323261/140‑1 (MQ=255)

                                                                                                                                  aCAAAGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATa        >  2:3605399/1‑140 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:5459702/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:6421010/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:4665543/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:7750626/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:9872668/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  2:10992741/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:3187145/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:1337779/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCCCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg    <  1:11230812/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑CCCCCCA‑TCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCGTC  >  NZ_CP025268/2292451‑2292725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`