BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	95.7%	1851.5 / 70.1	560	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (4/20); new base C (246/288); total (250/310)
Fisher's exact test for biased strand distribution p-value = 5.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 581 total aligned reads displayed.

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑G‑C‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

 cgtGTCTGCTGGCGAATAATTACGCAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGCAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGGAGACGATCCTGCCCTATAGTTGGAGT‑G‑c‑c                                                                                                                                        <  2:4497322/138‑1 (MQ=255)

    gTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGTACATCATCCCCCATTTTGTGGACTGCCCCCCAGGTAGAGGATCCTGCCGTATGGTGGGTGT‑G‑c‑cc‑cc                                                                                                                                    >  1:599734/1‑139 (MQ=255)

                   atTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGAGGATCCTGCCCGATAGGTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTaa                                                                                                                     >  1:6752468/1‑140 (MQ=255)

                   atTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGGAGACGATCCTGCCCTATAGGTGGGGT‑GCC‑CC‑C‑GATTCTGGTGGCTaa                                                                                                                     >  1:6493967/1‑140 (MQ=255)

                          aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGc                                                                                                              >  1:8976137/1‑140 (MQ=255)

                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCa                                                                                                             <  1:5053798/140‑1 (MQ=255)

                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCa                                                                                                             <  1:7101095/140‑1 (MQ=255)

                           gTATCGAGGTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCa                                                                                                             <  2:5248685/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGCTCATGAAGAGGGTACCGAAACGAGGGACATCATCCCCCAATTTGTGGACTGCCCCCCCGGTAGACGATCCGGCCCTATAGTTGGTGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAAGTTAGCAAAt                                                                                                          <  2:8049819/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  1:4525389/140‑1 (MQ=255)

                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TAGTCAGGTGGCTAAATTTAGCAAATc                                                                                                         >  1:8469111/1‑140 (MQ=255)

                               cGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATc                                                                                                         >  1:2895894/1‑140 (MQ=255)

                                   tttctgctTTGTGAGATCAGGTTGAGGTTACCGAAACGATTGTCATCATAACCCATTTTGTGTAATGACCCCCCGGTAGACGATCCTGCCCTATAGTTGGTGT‑GAC‑CA‑C‑TATTCTGGTGGCTAAATTTAGCATATCAATa                                                                                                     <  2:6052955/137‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:7451995/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:866639/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:5509725/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:274371/140‑1 (MQ=255)

                                   gtgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑GATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:10835079/139‑1 (MQ=255)

                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATac                                                                                                    >  1:9515489/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATGTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGGTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  2:10473937/1‑140 (MQ=255)

                                        gctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACtt                                                                                                <  1:7994300/140‑1 (MQ=255)

                                          ttGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                              <  2:3701396/140‑1 (MQ=255)

                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                                                                          <  2:6357131/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TCTTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        >  1:4444932/1‑140 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:10557173/140‑1 (MQ=255)

                                                  ttCAGGAAGCGGCTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCACCCGCTATACGATCCTGCCATATAGTAGTAGT‑G‑C‑CC‑CATAGTCATGTGGCTAAATTTATCAAATCAATACCCTTCTGGTGGGta                                                                                      <  2:1516247/139‑1 (MQ=255)

                                                  aTCAGGCAGAGGGTACCGATACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTTGACGATACTGCCCTATAGTTGGTGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:9410665/140‑1 (MQ=255)

                                                  aTCAGGAAGAGTTTACCGAACCGATGGACATCATCCCCCATTATGTGGACTGACACCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTCGCAAATCAATACACTTCCGGGGGGta                                                                                      <  2:3075372/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAATCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:10240380/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  1:10365905/140‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATATTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      <  2:11196305/140‑1 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTTTCGTTGGAGT‑GCC‑CC‑C‑TGTTCTGGTGGGTACATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     >  1:3950686/1‑140 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     >  1:4112737/1‑140 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     <  1:758005/140‑1 (MQ=255)

                                                       gAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTc                                                                                               <  1:2265428/126‑1 (MQ=255)

                                                        aaGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTg                                                                                <  1:10224678/140‑1 (MQ=255)

                                                          gagGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa                                                                              <  2:1743249/140‑1 (MQ=255)

                                                            ggTTACCGAAACGCTGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGAGGATGCTGCCCGATAGTTGTTGT‑GCC‑CC‑C‑TATTCAGGTGGCTATATGTAGCAAATCAAGAGACGTCGGGGGGGTATTATTGTaga                                                                            >  2:976951/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:6926291/128‑1 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            <  2:4880971/140‑1 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  2:11403569/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  1:1717724/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  1:4664869/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga                                                                            >  2:5105850/1‑140 (MQ=255)

                                                            ggTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TAGTCAGGTGGCTAAATTTAGCAAATCAATACACGTCAGGGGGGTATTATTGTaga                                                                            >  1:4277334/1‑140 (MQ=255)

                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTagag                                                                           >  1:9873440/1‑140 (MQ=255)

                                                              ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                          <  1:11585849/140‑1 (MQ=255)

                                                              ttACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGt                                                                          <  1:3315818/140‑1 (MQ=255)

                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg                                                                                             >  2:6484876/1‑118 (MQ=255)

                                                                 ccGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTc                                                                       >  1:11062173/1‑140 (MQ=255)

                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TCTTCAGGTGGCTAAATTTAGCAAATCAATACACTTCCGGGGGGTAGTATTGTAGAGTTTcc                                                                      >  1:10016611/1‑140 (MQ=255)

                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  1:8158599/140‑1 (MQ=255)

                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTGGGAGT‑GCC‑CC‑C‑TAGTCAGGTGGCTAAATGTAGCAAATCAGTGCACTGCAGGGGGGGCTTAGTGGAGAGTGTcc                                                                      >  1:6448345/1‑140 (MQ=255)

                                                                  cGAAACGATGGACATCAACCCCCATTTTGTGGTCTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑G‑C‑CCAC‑TATTCAGGTGTCTAAATTTAGCAAATCAAGACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  2:7183730/140‑1 (MQ=255)

                                                                      aCGATGGACATCCTCCCCCATTTTGAGGACAGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGTAGT‑G‑C‑CC‑CATATTCATGTGGCTAAAATTAGCAAATCAATACACTTAAGGGTGGTCTTATTGTTGAGTTTCCCCat                                                                  >  2:10396113/1‑140 (MQ=255)

                                                                       cGATGGACATCATCCCCCATTTTGTGGACAGCCCCCCAGTTACACGCTCCTGCCCTATCTTTGGAGT‑GCC‑CC‑C‑TTTTCATGTGGCTAAACTTAGCAAATCTCTTCACTTCAGGGTGGTATTATTGTACAGTTTCCCCtta                                                                 <  2:2947183/140‑1 (MQ=255)

                                                                        gATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGCGT‑GCC‑CC‑C‑TATTCAGGTGGCTCAATTTAGCAAATCAATACACTTCAGGGGGGTCTTATTGTAGAGTTTCCCCatat                                                                >  2:10667380/1‑140 (MQ=255)

                                                                         aTGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATg                                                               <  1:3683147/140‑1 (MQ=255)

                                                                          tGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAACTTTAGCAAATCAATACACTTCCGGGGGGTATTATTGTAGAGTTTCCCCATATGt                                                              <  2:4137872/140‑1 (MQ=255)

                                                                              catcatCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCt                                                          <  2:8558101/140‑1 (MQ=255)

                                                                                tcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAt                                                        <  1:1903478/140‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTAAGCAAATCAATACACGTCAGGGGGTTAGTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       >  2:9254571/1‑140 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGGAGACGATCCTGCCCTATAGTTGGAGT‑G‑CACC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAAGACACTTCAGGGGGGTATTATTGTAGAGTATCCCCATATGTTTCTATg                                                       <  2:793361/140‑1 (MQ=255)

                                                                                  atcCCCCTTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCGCGAGAGTTGGTGTGG‑C‑GC‑G‑TAGTCAGGTGGCTGAATTTAGCATAGCAATACACGGGAGGGGGGTATTATTGTAGAGTTTCCGAATATGTGTGTATgg                                                      >  2:8349183/1‑140 (MQ=255)

                                                                                             tgtgGACTGCCCCCCCGGGAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TCTTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa                                           <  2:5584823/140‑1 (MQ=255)

                                                                                                 gACTGCCCCCCCGGTAGACGATCCTGCCGTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTAGTGTAGAGTTTCCCCATATGTTGCTATGGGATCCAGGAATTGAc                                       >  2:3277352/1‑140 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGACATGACAAt                                    >  1:9205776/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  1:250799/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  2:260931/1‑140 (MQ=255)

                                                                                                       ccccccGGTAGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg                                 <  1:3596968/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTCTTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAACTGACAATCAGGAGTTACaa                        >  2:8751711/1‑140 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:9388464/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:7249959/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:3800187/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        >  1:11207227/1‑140 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTGTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        >  1:5322385/1‑140 (MQ=255)

                                                                                                                   cGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCTATACACGTCAGGGGTGTATTATTGTAGAGGTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaaa                     <  2:7284106/140‑1 (MQ=255)

                                                                                                                   cGATCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaaa                     <  1:5832245/140‑1 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  2:132118/1‑140 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  1:3096287/1‑140 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCAGATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt                   >  2:460547/1‑140 (MQ=255)

                                                                                                                      tCCTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACtt                  >  2:2349966/1‑140 (MQ=255)

                                                                                                                       ccTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGNAATGACAATCAGGAGTTACAAAAACTTa                 >  1:6276433/1‑140 (MQ=255)

                                                                                                                       ccTGCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa                 >  2:4385484/1‑140 (MQ=255)

                                                                                                                          gCCCTATAGTTGGAGT‑GCC‑CC‑C‑TATTCACTTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGTTATTATTGTAGAGTTTCCCCATATGTTACTATGGTATCCAGGAAAAGACAATCAGGAGTTACAAAAACTTAAAt              <  2:9048627/140‑1 (MQ=255)

                                                                                                                            ccTATAGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCt            >  2:353095/1‑140 (MQ=255)

                                                                                                                              tataGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACGTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTgg          >  2:3952926/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             <  1:1374548/135‑1 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:9970075/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:2161352/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:564989/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:48245/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:7751648/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:8896822/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAACTGGTc        >  1:6382931/1‑140 (MQ=255)

                                                                                                                                    tGGAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAggg    <  1:11410730/140‑1 (MQ=255)

                                                                                                                                     ggAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  2:5884992/1‑140 (MQ=255)

                                                                                                                                     ggAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  2:10907584/1‑140 (MQ=255)

                                                                                                                                     ggAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:9697671/1‑140 (MQ=255)

                                                                                                                                      gAGT‑GCC‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:3500623/140‑1 (MQ=255)

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGT‑G‑C‑CC‑C‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`