BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	98.0%	1878.3 / 21.7	508	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (5/5); new base T (236/260); total (242/266)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 590 total aligned reads displayed.

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑TTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

aCGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑at‑a                                                                                                                                          <  1:7632921/140‑1 (MQ=255)

    ggcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACTAAGTATTTTGTGAAACCTGCCCGAAGCTGGACGCAGGTGGGCTATACCCTATATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑t                                                                                                                                    >  2:1652354/2‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑t                                                                                                                                    >  1:9607396/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑t                                                                                                                                    >  1:9514120/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑t                                                                                                                                    >  1:9159156/1‑140 (MQ=255)

    agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACGATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑t                                                                                                                                    >  2:2798140/1‑140 (MQ=255)

    agcaTTTTGTCGCGACCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAATCCTTGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑GT‑A‑ttt‑t                                                                                                                                    >  2:1802228/1‑140 (MQ=255)

      caTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑ttg                                                                                                                                  >  1:1875305/1‑138 (MQ=255)

        ttttGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑ttt‑ttggt                                                                                                                                <  2:5364294/140‑5 (MQ=255)

            gTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCt                                                                                                                             <  1:11110405/140‑1 (MQ=255)

                 gcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGaa                                                                                                                        <  1:10682588/140‑1 (MQ=255)

                     tGATACGCTGCATTGCAGTTACGTAGAATTTTGTGAAACCTGGCCGGAGCTGGACGCAGGTGGGCTATACCCAATATTTGGTCACTAGTGCCTGAGGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTa                                                                                                                    <  2:10286532/140‑1 (MQ=255)

                      gATACGCTGCATTGCAGTTACGTAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTAt                                                                                                                   <  2:7301105/140‑1 (MQ=255)

                      caTACGCTGCATTGCAGTTACGAAGAATTTCGTGAAACCTGGCCGCAGCTGGACGCAGGGGGGCGATACCCACAATTTGGACACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ACTTT‑TGGTGGCTGTGAATTTAt                                                                                                                   <  2:3612738/139‑1 (MQ=255)

                       aTACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCATGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTAtt                                                                                                                  >  2:1961015/1‑140 (MQ=255)

                            cTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCACAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTa                                                                                                             <  2:5563403/140‑1 (MQ=255)

                             tGCATTGCAGTTCCGAAGAAGTTGGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCCAAAGTTGGTCACGATTGCCTGATGCCACTGGCGGCGGACGGGC‑ATCT‑TTT‑TGGTGGCGGTGAATTTCTTCGttct                                                                                                            >  2:10943056/1‑138 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGTCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:3047581/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCt                                                                                                                             >  2:9289777/1‑123 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:256570/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:5225689/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:9765800/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  1:10876496/1‑140 (MQ=255)

                             tGCATTGCAGTTACGAAGAATGTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AA‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                            >  2:6959973/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:2621130/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:10360164/1‑140 (MQ=255)

                               cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                          >  1:5406183/1‑140 (MQ=255)

                                 ttGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACg                                                                                                        >  2:9520292/1‑140 (MQ=255)

                                   gcccttACGAACAATACTGTGACACCTCGCCGCAGCAGGACGCAGGTGGTCTATACCAAAAATTTGGTCACTATTGCCTGATGCCACTCGCGGCGGAAGGGCAAT‑A‑TTT‑TGCTGGCTGAGAATTTATTCGATATCACGAt                                                                                                      <  2:5161742/136‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:10669457/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:2796475/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:664796/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  2:7857840/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:847771/140‑1 (MQ=255)

                                   gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                      <  1:10029706/140‑1 (MQ=255)

                                     aGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCg                                                                                                    <  1:3620568/140‑1 (MQ=255)

                                         actaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:9886802/4‑140 (MQ=255)

                                         acgacgAATTTTGTGAAACCTGGCCGCAGCTGGACGCCGGTGGGCTATACCCAAACTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AG‑ATTTT‑TGGTGGCTGTGAATGTATTCGTTATGACGATCGcccc                                                                                                <  2:2138395/140‑1 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                <  2:2420156/140‑1 (MQ=255)

                                         aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                                >  2:3851915/1‑140 (MQ=255)

                                           gaagaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTg                                                                                              <  1:4094291/140‑1 (MQ=255)

                                               aaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGc                                                                                          >  1:2220900/1‑140 (MQ=255)

                                                 ttttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa                                                                                        >  1:6022382/1‑140 (MQ=255)

                                                   ttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                                      <  1:3370674/140‑1 (MQ=255)

                                                   ttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaa                                                                                      <  1:5787050/140‑1 (MQ=255)

                                                     gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa                                                                                    <  1:8693511/140‑1 (MQ=255)

                                                     gtgAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTAGTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGaaaaa                                                                                    <  1:1268878/140‑1 (MQ=255)

                                                       gAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAaga                                                                                  <  1:6440465/140‑1 (MQ=255)

                                                       gAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAaga                                                                                  <  2:4151410/140‑1 (MQ=255)

                                                              ggCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAAt                                                                           <  2:8049753/140‑1 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGGCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  1:8423787/1‑140 (MQ=255)

                                                                ccGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCg                                                                         >  1:292133/1‑140 (MQ=255)

                                                                 cGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGt                                                                        <  1:2399584/140‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  2:5695194/140‑1 (MQ=255)

                                                                      cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                   <  1:10237967/140‑1 (MQ=255)

                                                                         gACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAAc                                                                >  1:8018535/1‑140 (MQ=255)

                                                                                gcgggCTATACCCAAAATTTGGTCACTATGGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGTTGGCTGTTAATTTATTCGTTATGACTATCGCCACTGGAGCGAAAAAGAGTTCAATCGTAAGCAAACATTTACg                                                         <  2:583585/138‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         <  2:7547486/140‑1 (MQ=255)

                                                                                gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                         >  1:11427335/1‑140 (MQ=255)

                                                                                        taCCCCAAAGTTGGTCCCTATTGCCTGTTGCCACTGGCGGCGGAAGGGC‑AT‑A‑TTTGTGGTGGCTGTGAATTTATTCGGTATGACTATCGCCCCTGGAGCGAAAAAGAGTTCAAGCGTCTGCAATCATGTACGCCGATCGt                                                 >  2:7106522/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGTGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:3801377/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  1:4024984/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  2:11453205/1‑140 (MQ=255)

                                                                                        taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                 >  2:11219722/1‑140 (MQ=255)

                                                                                          cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                               >  1:8137882/1‑140 (MQ=255)

                                                                                             aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAATAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTg                                            >  1:6602003/1‑140 (MQ=255)

                                                                                             aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTg                                            >  2:8738546/1‑140 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  1:7780492/140‑1 (MQ=255)

                                                                                              aaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgt                                           <  2:8261800/140‑1 (MQ=255)

                                                                                                    ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCGGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                     <  2:4359790/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:42892/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:11219723/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  1:8898036/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:10206677/140‑1 (MQ=255)

                                                                                                         cTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                                <  2:10795059/140‑1 (MQ=255)

                                                                                                                cTGATGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCa                         <  2:11065201/140‑1 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGACGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCGGCAAACATTTACGCCGATCGGTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:3346280/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGATGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:10520191/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:5615259/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:4908882/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  2:2921143/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:8264834/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:9317015/1‑140 (MQ=255)

                                                                                                                    tGCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                     >  1:6817921/1‑140 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  1:1612067/140‑1 (MQ=255)

                                                                                                                     gCCACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                    <  1:1056404/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TNGTGGCTGNGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCNCCGAACAAATCCAGAGCcgc                  <  1:6248543/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGATCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCTAACAAATCCAGAGCcgc                  <  2:6156087/140‑1 (MQ=255)

                                                                                                                       cACTGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgc                  <  1:9107452/140‑1 (MQ=255)

                                                                                                                          tGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgtc               <  1:310896/140‑1 (MQ=255)

                                                                                                                          tGGCGGCGGAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTACTGTCGTCACCGAACAAATCCAGAGCCGcgtc               <  2:8952917/140‑1 (MQ=255)

                                                                                                                             cggcggAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGtt            <  2:6022381/140‑1 (MQ=255)

                                                                                                                             cggcggAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGtt            >  1:5077104/1‑140 (MQ=255)

                                                                                                                              ggcggTAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           <  2:4033282/140‑1 (MQ=255)

                                                                                                                              ggcggAAGGGC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa           >  1:4719307/1‑140 (MQ=255)

                                                                                                                                     gggC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  2:6376830/140‑1 (MQ=255)

                                                                                                                                     gggC‑AT‑ATTTT‑TGGGGGCTGTGAATTTATTCGTTATGCCGTTCGCCCCTGGAGCGAAAAAGAGTTCCATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGt    <  2:9060397/140‑1 (MQ=255)

                                                                                                                                      ggC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTc   <  2:2617577/140‑1 (MQ=255)

                                                                                                                                      ggC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTc   >  2:1511885/1‑140 (MQ=255)

                                                                                                                                       gC‑AT‑ATTTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCg  >  1:7411422/1‑140 (MQ=255)

ACGCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGC‑AT‑A‑TTT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTCG  >  NZ_CP025268/2858609‑2858882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`