BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,426,468	+C	pseudogene (249/275 nt)	yjfY ←	DUF1471 family protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,426,468	1	.	C	99.7%	2051.2 / ‑5.4	592	pseudogene (249/275 nt)	yjfY	DUF1471 family protein YjfY
Reads supporting (aligned to +/- strand): ref base . (0/1); major base C (314/275); minor base A (1/1); total (315/277)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 608 total aligned reads displayed.

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

        cctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGc                                                                                                                                >  1:6682046/1‑140 (MQ=255)

              gagaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTAc                                                                                                                          <  1:2682143/140‑1 (MQ=255)

                gaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTc                                                                                                                        >  1:2932328/1‑140 (MQ=255)

                  tAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTccc                                                                                                                      <  2:4971009/140‑1 (MQ=255)

                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTcccc                                                                                                                     <  2:1493012/140‑1 (MQ=255)

                    aCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCAATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCg                                                                                                                    >  2:11563197/1‑140 (MQ=255)

                    aCTGGTCTAAGTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCg                                                                                                                    <  2:10553649/140‑1 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:8092445/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTc                                                                                                                >  2:955496/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTc                                                                                                                >  2:9083752/1‑140 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:5989367/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  2:2245057/140‑1 (MQ=255)

                             aTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCa                                                                                                           >  1:5876372/1‑140 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          <  1:8507641/140‑1 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          <  2:6920666/140‑1 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          >  1:11590643/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATACATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     <  1:10700396/140‑1 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  2:685713/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCGGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  2:108712/1‑140 (MQ=255)

                                      tctattttAAAAACAAAGCCCCCTTCGAAGGGTTCAAAGCAACCTATTGCAATGTTTCGTTGGTTATACCTGGTTCTAGGGTTTAATTTTCGAGGTGGCGTGAATATTGGACTTACTCCTAGGTTCACTCATCATAATTa                                                                                                  <  2:3978831/136‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCATAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCAGCGCTGGCGTGCATATTGCAGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:11271473/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:5181917/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:1628503/140‑1 (MQ=255)

                                        gattttCAAAAAAAAGCCCCCTGCGATGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:10115348/138‑1 (MQ=255)

                                        gTTTTTCAAAACAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3781369/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:11315630/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:4250962/140‑1 (MQ=255)

                                        gTTATTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:10293480/140‑1 (MQ=255)

                                           tttCAAAAACAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTGATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTgg                                                                                             <  1:3637052/140‑1 (MQ=255)

                                           tttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTgg                                                                                             <  1:11200649/140‑1 (MQ=255)

                                                aaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtcgta                                                                                        >  2:7909922/1‑140 (MQ=255)

                                                   caaaGCCCCCTGCGAAGGGGGCAAAGCAACCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGt                                                                                     <  2:7044689/139‑1 (MQ=255)

                                                   aaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGt                                                                                     >  1:8915433/1‑140 (MQ=255)

                                                         ccccTGCGAAGGGGGCAAAGCAAACTCTGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGcc                                                                               <  2:10645141/140‑1 (MQ=255)

                                                         ccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGcc                                                                               <  1:4937597/140‑1 (MQ=255)

                                                            cagCGACGGGGGCAAAGCCCACTCTCGCCATGTTTCGTTGGTAATACCTGGTGCTAGCGATAACTATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCAACAGAATTACGTGGTCGGACGTTGCGCCTTg                                                                            <  2:11394129/138‑1 (MQ=255)

                                                            cTGCGCAGGGGGCAAAGCAAACTATCGCAATTTTTCGTTGGTTATCCCAGGTTCTAGCGAGAAAAATCCGCGCTTGCGTGCATATTGCCGTTACGCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                                            <  2:6056359/140‑1 (MQ=255)

                                                            cTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                                            <  1:8740928/140‑1 (MQ=255)

                                                                     gggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGt                                                                   >  2:7726265/1‑140 (MQ=255)

                                                                      ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                                                  >  1:9396623/1‑140 (MQ=255)

                                                                           agcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttcttc                                                             >  1:3423985/1‑140 (MQ=255)

                                                                                aCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTa                                                        >  2:5854749/1‑140 (MQ=255)

                                                                                     ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                                                   >  2:7487255/1‑140 (MQ=255)

                                                                                       cAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTg                                                 >  1:7829906/1‑140 (MQ=255)

                                                                                       cAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTg                                                 >  2:9721654/1‑140 (MQ=255)

                                                                                        aaTGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGGATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGa                                                >  2:6714298/1‑140 (MQ=255)

                                                                                         aTGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGAc                                               >  2:3100505/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  1:721699/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  2:8947665/1‑140 (MQ=255)

                                                                                           gTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGt                                             <  2:3314183/140‑1 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  2:430237/1‑140 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  1:7444881/1‑140 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  2:11182898/1‑140 (MQ=255)

                                                                                             ttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGc                                           <  1:11074696/140‑1 (MQ=255)

                                                                                              tCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  2:293451/140‑1 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  <  2:6178438/140‑1 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  1:6834884/1‑140 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGATGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  2:1347557/1‑140 (MQ=255)

                                                                                                        taCCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTGCTCCCCTGTTCCCGCATCAGAATTGCGTGGTAGTAAGTTGCGCCGGGCGCCTCTGTGTCTTCATGTAATGCCTGTCGTGCTTCGCTGTCAg                                >  2:733279/1‑140 (MQ=255)

                                                                                                           cTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTgg                             <  1:6438623/140‑1 (MQ=255)

                                                                                                            tGGTGCTAGCGATAAATATCCGCGCGGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc                            <  1:8987315/140‑1 (MQ=255)

                                                                                                             ggTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCg                           <  1:3244136/140‑1 (MQ=255)

                                                                                                             ggTGCTAGCGATAAATATCCGAGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCGTGCGCATCTGTTTCTTCATTTAATGCATGACGTGCTTCGCTTTCAGTGGCg                           <  2:2069614/140‑1 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:1889121/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:2460241/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:5174900/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:8125818/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:8634035/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:5711343/1‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  1:8163652/1‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  1:4477839/4‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGATCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  1:4621888/4‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGAGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTAGCTTTCAGTGGCGaa                         >  2:9879385/4‑140 (MQ=255)

                                                                                                                  tAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  >  2:3535121/1‑64 (MQ=255)

                                                                                                                  tAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:3535122/64‑1 (MQ=255)

                                                                                                                   aGCGATAAATATCNGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGANGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:6258271/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  2:10757225/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    >  1:2516370/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGAGGCGAAATTAt                    >  1:3884859/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTGGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    >  1:3099964/1‑140 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:11534354/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:2732502/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:9319482/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:2134019/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:9855885/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:763088/140‑1 (MQ=255)

                                                                                                                        tAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgatt                <  1:6499402/140‑1 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  1:10504345/1‑140 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  1:5545201/1‑140 (MQ=255)

                                                                                                                              tCCGCGCTGGCGTGCATATTGCCGTTACTCCCGGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata          >  1:9630010/1‑140 (MQ=255)

                                                                                                                              tCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata          >  1:40388/1‑140 (MQ=255)

                                                                                                                              tCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGAtata          >  1:10859999/1‑140 (MQ=255)

                                                                                                                               ccGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaa         >  2:4839002/1‑140 (MQ=255)

                                                                                                                                cgcgCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa        >  1:8319924/1‑140 (MQ=255)

                                                                                                                                cgcgCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa        >  2:5924757/1‑140 (MQ=255)

                                                                                                                                cgcgCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATaaa        >  2:10330831/1‑140 (MQ=255)

                                                                                                                                 tcgcTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  2:4518262/139‑1 (MQ=255)

                                                                                                                                  cgcTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  2:8386362/1‑140 (MQ=255)

                                                                                                                                   gcTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCa     <  1:2051895/140‑1 (MQ=255)

                                                                                                                                    cTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTACATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc    >  2:6427917/1‑140 (MQ=255)

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`