Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2250TA23.0% 349.2 / 43.6 197intergenic (+125/+248)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (54/97);  new base A (45/0);  total (99/98)
Fisher's exact test for biased strand distribution p-value = 8.89e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 200 total aligned reads displayed.

AAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGG  >  NZ_CP025268/2233086‑2233364
                                                                                                                                           |                                                                                                                                            
  gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcg                                                                                                                                            <  1:9473853/140‑1 (MQ=255)
        ctttaAAAATTAATTAATGACCTCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccg                                                                                                                                      <  2:11341692/139‑1 (MQ=255)
        atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccgccg                                                                                                                                      <  2:8219742/140‑1 (MQ=255)
          tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGGCCATAgccgccccc                                                                                                                                    >  1:7531260/1‑140 (MQ=39)
          tttAAAATTAATTAATGACATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGGTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                                                    <  2:3935814/140‑1 (MQ=255)
                attaattaATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAACCCCGCCGCCCCC‑AGc                                                                                                                             >  1:11611196/1‑140 (MQ=255)
                 ttaattcaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTAATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCa                                                                                                                            <  2:8322677/140‑1 (MQ=255)
                         tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCggg                                                                                                                    <  1:5082078/140‑1 (MQ=255)
                           tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGct                                                                                                                  <  2:1331104/140‑1 (MQ=255)
                              tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTg                                                                                                               <  1:11104206/140‑1 (MQ=255)
                              tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTg                                                                                                               >  2:5037673/1‑140 (MQ=255)
                                  gTCCGAAAATAACGAGAATATTTCAGTCTCTGCTCCTGTTGCGCTCCTGTCATGTGCATTGCGTCATATAATCACTGGCGCCAGGGGCGCGGAGGGGGCGGCCCTTCGCCGCCGCCCCG‑TGCACCCCCGGGGGCTGGCgg                                                                                                           >  2:7213671/1‑139 (MQ=255)
                                   tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCATCCCCCCCCCCCCC‑AGCACCCCCCCGCTCAGGCgga                                                                                                          >  1:7498636/1‑138 (MQ=25)
                                    ccGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGCCCAAACCCGCCGCCCCC‑AGCACCCCCCGGCGCTGGCGAAc                                                                                                         >  1:9227459/1‑140 (MQ=25)
                                     cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGCCCATCCCCCCCCCCCCC‑CGCACCCCCCGGCGCGGGCGAACa                                                                                                        >  1:4085178/1‑140 (MQ=25)
                                     cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACa                                                                                                        >  2:9708205/1‑140 (MQ=255)
                                     cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAACCCCCCCCCCCC‑ACCACCCCCCCGCACAGGCGAACa                                                                                                        >  1:11452839/1‑140 (MQ=25)
                                       aaaaTAACGAGACTCTTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATAGCCGCCGCCCCC‑GGCACCCCCGGGCGCTGGCGAACaaa                                                                                                      >  2:11077431/1‑140 (MQ=21)
                                       aaaaTAACGAGAATCTTTCAGTCTGTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCTAGGAGCGCGCTGGGGGCGGGCCTTCGCCGCCGCCCCG‑AGCACCCCCGGGCTCTGGCGACCaca                                                                                                      >  2:10085779/1‑138 (MQ=21)
                                            aaCGTGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCAATCGCCGCCCCCCCCGGGCACCCCC‑GGCGCGGGCGCACAAAAAcgc                                                                                                 >  1:847071/1‑140 (MQ=21)
                                             aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGGGCCAAAAGCCCCCCCCCCC‑CGCACCCCCCCGCTCAGGCGGACaaaaaaacc                                                                                                >  1:6135615/1‑135 (MQ=255)
                                                  aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCC‑TGCACCCCCCGGCTCTGGCGAACAAAAAAGCCGCGgc                                                                                           >  2:10786141/1‑140 (MQ=21)
                                                       ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt                                                                                      <  2:7886966/140‑1 (MQ=255)
                                                         cAGGCTCGCAGCCGGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGGCACTCGGCGGCGCCCCC‑GGCACGCCCGGGCTCTGGCGGACATAATCGCGGGGGCGCGGTGc                                                                                    >  2:8112137/1‑140 (MQ=18)
                                                           gtatctcAGCCTGTTGGGCTCCTGTAATGTGATTTGCTTCATATAATCACTGGGGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                  <  1:1405286/137‑1 (MQ=17)
                                                           gTCTCTCATCCTGTTGCGCTCCTGTCCTGTGCATTGCTTCATATAATCACTGGCGCCAGGAGCGCGCCGGGGGCGGCCACGCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGccc                                                                                  <  2:1627125/140‑1 (MQ=18)
                                                           gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                  <  2:3316720/140‑1 (MQ=255)
                                                            tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGCGGCCAAACGCCCCCCCCCCC‑AACACCCCCCGGCACAGGCCAAAAAAAACGCCCCAGCGCCGTGcccc                                                                                 >  1:6983980/1‑139 (MQ=255)
                                                            tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCAGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCAGGCGAAAAAAATAGCCGCTGCGCGGTGccca                                                                                 >  2:6724952/1‑139 (MQ=18)
                                                            tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGCCAAAAGCCGCCGCCCCC‑AGCCCCCCCCCGCACAGGCGAACAAAAAAGCCGCAGCGCGGTGccca                                                                                 >  2:1501377/1‑139 (MQ=18)
                                                            tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAAACGCCGCAGCGCGGTGCCCt                                                                                 >  2:6986245/1‑140 (MQ=255)
                                                                tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                             <  1:6474130/140‑1 (MQ=255)
                                                                tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                             <  2:3944956/140‑1 (MQ=255)
                                                                   tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                          <  1:4708925/140‑1 (MQ=255)
                                                                    ccTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                         <  2:8877993/140‑1 (MQ=255)
                                                                      tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                       <  1:8094552/140‑1 (MQ=255)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGCGGCCCAAAACCGCCCCCCCC‑AGCCCCCCCCGGCACATGCGAACAAAAAAGCCGCAGCGCCGTGGCCACCGCGTATcccg                                                                     >  2:1467783/1‑139 (MQ=255)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATAGCCGCCCCCCCC‑AGCCCCCCCCGGCACAGGCGAACAAAAACCCCGCTGCGCGGTGCCCACGGCATATacca                                                                     >  2:5724595/1‑139 (MQ=18)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCCGGCTCTGGCGAACAAAAAACCCGCTGCGCGGTGCCCACGGCTTATccca                                                                     >  2:7548053/1‑139 (MQ=18)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCCCCCCC‑AGCACCCCCCCGCGCAGGCGAACAAAAAAGCCCCAGCGCGGTGCCCGCGGCGTATacca                                                                     >  1:10699196/1‑139 (MQ=18)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCCATAGCCGCCGCCCCC‑AGCACCCCCCGGCGCTGGCGAAAAAAAAAGCCCCTGAGCGGTggcctaggcctaaaccg                                                                     >  2:9094380/1‑135 (MQ=17)
                                                                        ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAAGCCGCCGCCCCC‑AGCACCCCCGCGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATccca                                                                     >  2:9341460/1‑139 (MQ=18)
                                                                          gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                   <  1:10035075/140‑1 (MQ=255)
                                                                          gcgcTCCTGTCATGTGCAATGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGGCGCCCCCCCC‑ATCAACCCCGGGCAAGGTCGAAACACAACGACGCAGCCCGGTGGCCACGGCGTATCCCATa                                                                   >  2:370083/1‑140 (MQ=12)
                                                                             cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACgg                                                                >  2:11501089/1‑140 (MQ=255)
                                                                             cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCGCCCCC‑AGCACCCCCCGGCACTGGCGAACAAAAACGCCGCTGCGCGGTGCCCACGGCATATCCCATAccg                                                                >  2:8548971/1‑138 (MQ=18)
                                                                             cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCCGCCAATAGCCGCCGCCCCC‑ATCACCCCCGGGCACTGGCGAACAAAAACGCCGCTGCGCAGTGCCCTCGGCGTATCCCGAACgg                                                                >  2:9738391/1‑140 (MQ=18)
                                                                                cTGTCATGTGCATTGCTTCATATAATCACTGGCGCTAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATAGCCGCTGCGCGGTGCCCACGGCTTATGCCTTACGGCTa                                                             >  2:11112568/1‑140 (MQ=17)
                                                                                  gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGGCAAAATCCCCCGCCCCC‑ACCCCCCCCCGGCGCTTGCGGACCAAAAAACCCCAGCGCCGGGCCCACGGCGTATACCATAAGGCAAcc                                                           >  2:5421685/1‑140 (MQ=255)
                                                                                        tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGCGGCCAAAAGCCGCCCCCCCC‑CGCACCCCCGCGCTCTGGCGAACAAACAACCCCCAGCGCCGTGCCCACGGCATAACACAGACGGCGAACCGGTCg                                                     >  2:6328184/1‑140 (MQ=9)
                                                                                        tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCCGCTTATCCCGTACGGCTACCGGGTCg                                                     >  2:10385932/1‑140 (MQ=255)
                                                                                        tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACCCCCCCCCCCCC‑CGCACCCCCCGGCTCTGGCGCACACAATCACCGCAGCGCGGTGGCCGCGGCGGAACCCATACGGCTACCCGGTCg                                                     >  2:10578448/1‑140 (MQ=14)
                                                                                          cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCAATCTCCGCCCCCCCC‑CGCCACACCCGGCGCTTGCGAACAAAAAAGGCCCGTTGCGGGGGCCCAGGCGTATCCCGTAAGGCTACCGGGtgcgg                                                   >  2:101971/1‑136 (MQ=255)
                                                                                          cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                   <  1:8934118/140‑1 (MQ=255)
                                                                                          cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                   <  2:2105165/140‑1 (MQ=255)
                                                                                            ttGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                 <  2:9227386/140‑1 (MQ=255)
                                                                                             tGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCACCCCCCCGCACATGCGAACAAAAAATCCCCGGCGCGGTGCCCACCGCATATAACATAACGCTAACCGGGCGGgcgc                                                >  2:8073454/1‑140 (MQ=255)
                                                                                             tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  2:1264491/140‑1 (MQ=255)
                                                                                             tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  1:1873184/140‑1 (MQ=255)
                                                                                             tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                <  1:6048318/140‑1 (MQ=255)
                                                                                             tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCCCCGCCCCC‑TTCACCCCCCGGCTCTGGCGAACAAAATCGCCGCGGCGCGGGGCCCGCGGCTTATACCATACGGCAACCCGGGCGGgcgc                                                >  2:10301479/1‑140 (MQ=14)
                                                                                             tGCGTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCACTCGCCGCCGCCCCC‑TGCACCCCCGGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCCTACGGCTACCGGGTCGGgcgc                                                <  2:5963683/140‑1 (MQ=255)
                                                                                              ccTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                               <  2:1405358/139‑1 (MQ=255)
                                                                                               cTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCCAAAGCCCCCGCCCCC‑AGCAACCCCCGGCAAAGGCGCACAAAAAACCCCCAGCGCGGGGGCCACAGCGTATACCATACGGCGAACCGGGCGGGGGCGa                                              >  2:5604228/1‑140 (MQ=255)
                                                                                               cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                              <  2:2814840/140‑1 (MQ=255)
                                                                                               cTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAATAGCCCCCCCCCCC‑TGCACCCCCGGGGGCTGGCGCACAAAATCGCCGCTGCGCGGTGCCCTCGGCGGATCCCTTACGGCGACCCGGTCGGGcgcgc                                              >  2:5053298/1‑139 (MQ=14)
                                                                                                 tCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAACGCCCCCGCCCCC‑AGCACCCCCCGGCTCTGGCGAACAAAATAGCCGCATCGCGGTGCCCACCGCATATCCCATACGGCGACCCGGTCGGGcgcgccg                                            >  2:687809/1‑137 (MQ=14)
                                                                                                  cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACCAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCGTACGGCTACCCGGTCGGGCGCGAggg                                           >  2:7717734/1‑139 (MQ=14)
                                                                                                    tataATCACTGGCGCAAGGAGCGCGCCGGGGGCCGCCAAACGCCGCCCCCCCC‑AGCACCCCCCGGCTAAGGCGAACCAAATAGCCGCAGCGCCGTGCCCACCGCGTTAACCATACGGGAACCCGGACGGGCGCGAGGTaa                                         >  2:9227535/1‑140 (MQ=2)
                                                                                                      taATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                       <  1:2877769/140‑1 (MQ=35)
                                                                                                       aaTCACTGGCGCAAGGAGTGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                      <  2:9987460/140‑1 (MQ=21)
                                                                                                        aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                     <  1:5037331/140‑1 (MQ=33)
                                                                                                        aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                     <  1:8892645/140‑1 (MQ=33)
                                                                                                         tCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAAGCCCCCCCCCCC‑AGCCCCCCCCCGCACTGGCGCACAAAATACCCCCGGCGCGGGGGCCACCGCATATCCCAAAACGCAACCCGGTCCGGCGCGCGGTAACAAcc                                    >  2:4816052/1‑140 (MQ=2)
                                                                                                              ggCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCATACGGCTACCCGGTCGGGCGCGAGGGAACATCCCTGTa                               >  2:7672569/1‑140 (MQ=11)
                                                                                                              ggCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCGCGGCTTATACCTTACGGCGACCCGGGCGGGCGCGCGGGAACATCCCTgga                               >  2:7672511/1‑138 (MQ=11)
                                                                                                                  cAAGGAGCGCGCAGGGGGCGGCCAATCCCCGCCGCCCCC‑AGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCACGGCATATACCATACCGCAACCGGGTCGGGCGCGAGGGAACAACCCAGTAAAAc                           >  2:6215183/1‑140 (MQ=11)
                                                                                                                   aaGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                          <  2:9062081/140‑1 (MQ=32)
                                                                                                                    aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                         <  1:2459617/140‑1 (MQ=32)
                                                                                                                         cgcgcCGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  1:4816053/140‑1 (MQ=17)
                                                                                                                         cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  2:3512714/140‑1 (MQ=32)
                                                                                                                         cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  1:2986192/140‑1 (MQ=32)
                                                                                                                         cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                    <  1:3372444/140‑1 (MQ=32)
                                                                                                                           cgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCa                  <  1:3285458/140‑1 (MQ=32)
                                                                                                                             cAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                <  1:1993811/140‑1 (MQ=32)
                                                                                                                             cAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                <  1:7851726/140‑1 (MQ=32)
                                                                                                                              aGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc               <  2:6269377/140‑1 (MQ=32)
                                                                                                                                ggggCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca             <  2:2285983/140‑1 (MQ=32)
                                                                                                                                ggggCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca             <  1:5592359/140‑1 (MQ=32)
                                                                                                                                  ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca           <  1:6219560/140‑1 (MQ=32)
                                                                                                                                  ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca           <  1:9962259/140‑1 (MQ=32)
                                                                                                                                  ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca           <  1:4646747/140‑1 (MQ=32)
                                                                                                                                  ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca           <  1:9305293/140‑1 (MQ=32)
                                                                                                                                    cggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCGACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCCCATc         >  2:8623476/1‑140 (MQ=17)
                                                                                                                                     ggcCAAAAGCCGCCGCCCCC‑TGCACCCCCCGGCTCTGGCGAAAAAAAAACCCGCTGCGCGGTGCCCACGGCATATACCTTACGGCAACCCGGTCGGGCGCGAGGTAACATACCAGTAAAACGCGCCCACAGCCCACAAcc        >  1:6621536/1‑140 (MQ=11)
                                                                                                                                      gcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa       <  1:8827477/140‑1 (MQ=32)
                                                                                                                                      gcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa       <  1:7405965/140‑1 (MQ=32)
                                                                                                                                      gcCAATCGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATAAGGCTACCCGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa       >  1:3670131/1‑140 (MQ=255)
                                                                                                                                        cAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCGGGCGAACGGGATCGCCGCTGGGCGGTGCCCGCGGCTTATCCCGTACGGGTCCCGGGTCGGGGGCGAGGGAACATCCCGGTACTACGCGCCCTCAGCCCTCGTCCCtg     >  2:4313448/1‑140 (MQ=11)
                                                                                                                                          aTCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGAAAAACGCTCCCTCAGCCCACATCCAtgag   >  2:8738159/1‑138 (MQ=12)
                                                                                                                                           |                                                                                                                                            
AAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGG  >  NZ_CP025268/2233086‑2233364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑