Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2480GC22.5% 445.2 / 81.2 215intergenic (+148/+225)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base G (72/93);  new base C (48/0);  total (122/93)
Fisher's exact test for biased strand distribution p-value = 9.95e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 224 total aligned reads displayed.

TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACG  >  NZ_CP025268/2233111‑2233387
                                                                                                                                          |                                                                                                                                           
tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCggg                                                                                                                                           <  1:5082078/140‑1 (MQ=255)
 gTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCATGGGGCGGCCAAACGCCCCCGCCCCC‑AGCACCCCCGGGc                                                                                                                                          >  2:1486806/1‑140 (MQ=255)
  tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑GGCAACCCCCGGcg                                                                                                                                         >  1:9678615/1‑139 (MQ=255)
     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTg                                                                                                                                      <  1:11104206/140‑1 (MQ=255)
     tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCGCCCCC‑AGCACCCCCCGGCTCTg                                                                                                                                      >  2:5037330/1‑140 (MQ=255)
         gTCCGAAAATAACGAGAATATTTCAGTCTCTGCTCCTGTTGCGCTCCTGTCATGTGCATTGCGTCATATAATCACTGGCGCCAGGGGCGCGGAGGGGGCGGCCCTTCGCCGCCGCCCCG‑TGCACCCCCGGGGGCTGGCgg                                                                                                                                  >  2:7213671/1‑139 (MQ=255)
          tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGGCCATCGCCGCCGCCCCC‑AGCACCCCCCGGCGCTGGCGaa                                                                                                                                 >  2:11104205/1‑140 (MQ=25)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGCCCATCCCCCCCCCCCCC‑CGCACCCCCCGGCGCGGGCGAACa                                                                                                                               >  1:4085178/1‑140 (MQ=25)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGGCCCACGCCGCCCCCCCC‑AGCACCCCCCGGCGCTGGCGcaca                                                                                                                               >  1:9196456/1‑140 (MQ=25)
                   aaCGTGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCAATCGCCGCCCCCCCCGGGCACCCCC‑GGCGCGGGCGCACAAAAAcgc                                                                                                                        >  1:847071/1‑140 (MQ=21)
                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGGGGCCAAAAGCCCCCCCCCCC‑CGCACCCCCCCGCTCAGGCGGACaaaaaaacc                                                                                                                       >  1:6135615/1‑135 (MQ=255)
                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGCCCATACCCCCCGCCCCC‑CGCACCCCCCGGCTCTGGCGCACAAAATagcc                                                                                                                       >  1:7690772/1‑140 (MQ=21)
                         aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCCCCGCCCCC‑AGCCCCCCCGGGCTCAGGCGAACAAAATagccccagc                                                                                                                  >  2:1459432/1‑135 (MQ=21)
                                cAGGCTCGCAGCCGGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGGCACTCGGCGGCGCCCCC‑GGCACGCCCGGGCTCTGGCGGACATAATCGCGGGGGCGCGGTGc                                                                                                           >  2:8112137/1‑140 (MQ=18)
                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                         <  2:3316720/140‑1 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCAGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCAGGCGAAAAAAATAGCCGCTGCGCGGTGccca                                                                                                        >  2:6724952/1‑139 (MQ=18)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGCGGCCCCACCACCCCCCCCCC‑CCCACCCCCCCCCACAGGCGAACCAAAACACCCCAGCGCGGGGcccaccgc                                                                                                    >  1:2942681/1‑135 (MQ=16)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                                                    <  2:3944956/140‑1 (MQ=255)
                                           ccTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                                                <  2:8877993/140‑1 (MQ=255)
                                             tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                                              <  1:8094552/140‑1 (MQ=255)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATAGCCGCCCCCCCC‑AGCCCCCCCCGGCACAGGCGAACAAAAACCCCGCTGCGCGGTGCCCACGGCATATacca                                                                                            >  2:5724595/1‑139 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCCCCCCCCCC‑AGCACCCCCCGGCACAAGCGAACAAAAACACCCCAGCGCGGGGCCCACGGCatataaca                                                                                            >  2:1277278/1‑136 (MQ=16)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                                            <  1:4907055/140‑1 (MQ=255)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCCGGCTCTGGCGAACAAAAAACCCGCTGCGCGGTGCCCACGGCTTATccca                                                                                            >  2:7548053/1‑139 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGAGGCCAAAAGACCCCGCCCCC‑AGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGGGGTGGCCTCGGCTTTTGCCt                                                                                            >  2:8354540/1‑140 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAAAGCCGCCGCCCCC‑AGCACCCCCGCGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATccca                                                                                            >  2:9341460/1‑139 (MQ=18)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                                          <  1:10035075/140‑1 (MQ=255)
                                                 gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATAGCCGAGCCCCAC‑AGAACCTCCGGGCTATGTGGAACAAAATAGCCGGTCCGCGGTGCCCAAGGCTTATACCATa                                                                                          >  2:1565086/1‑140 (MQ=16)
                                                    cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTATCCCTTACgg                                                                                       >  2:11501089/1‑140 (MQ=255)
                                                     tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCTGGGGGGGGGCCATCGCCCCCGCCCCCATCCACCCCC‑GGCGCTGGCGTAAAAAATAGCCGCAGGGCGGTGGCCACGGCTTTTACCTTAgggg                                                                                      >  2:1376588/1‑139 (MQ=12)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGGGGGCAAAATCCCCCGCCCCC‑ACCCCCCCCCGGCGCTTGCGGACCAAAAAACCCCAGCGCCGGGCCCACGGCGTATACCATAAGGCAAcc                                                                                  >  2:5421685/1‑140 (MQ=255)
                                                             tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGCTACCgggg                                                                              >  2:8580440/1‑139 (MQ=255)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGCGGCCAAAAGCCGCCCCCCCC‑CGCACCCCCGCGCTCTGGCGAACAAACAACCCCCAGCGCCGTGCCCACGGCATAACACAGACGGCGAACCGGTCg                                                                            >  2:6328184/1‑140 (MQ=9)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACCCCCCCCCCCCC‑CGCACCCCCCGGCTCTGGCGCACACAATCACCGCAGCGCGGTGGCCGCGGCGGAACCCATACGGCTACCCGGTCg                                                                            >  2:10578448/1‑140 (MQ=14)
                                                                 cagtGCTTCATATAATCACTGGCGCACGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                          <  1:3134229/137‑1 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCAATCTCCGCCCCCCCC‑CGCCACACCCGGCGCTTGCGAACAAAAAAGGCCCGTTGCGGGGGCCCAGGCGTATCCCGTAAGGCTACCGGGtgcgg                                                                          >  2:101971/1‑136 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                          <  1:2299058/140‑1 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                          <  2:2105165/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGCGGAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCACCCCCCCGCACATGCGAACAAAAAATCCCCGGCGCGGTGCCCACCGCATATAACATAACGCTAACCGGGCGGgcgc                                                                       >  2:8073454/1‑140 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGGGGCCCAACGCCGCCGCCCCC‑AGCACCCCCCGGCTCTGGGGCACAAAAACGCCGCGGCGCGGTGCCCGAGGCTTTTACCATAAGGCTACCCGGTCgggggg                                                                       >  2:9068543/1‑137 (MQ=9)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                       <  1:6048318/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCCCCGCCCCC‑TTCACCCCCCGGCTCTGGCGAACAAAATCGCCGCGGCGCGGGGCCCGCGGCTTATACCATACGGCAACCCGGGCGGgcgc                                                                       >  2:10301479/1‑140 (MQ=14)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGCCCAAAGCCCCCGCCCCC‑AGCAACCCCCGGCAAAGGCGCACAAAAAACCCCCAGCGCGGGGGCCACAGCGTATACCATACGGCGAACCGGGCGGGGGCGa                                                                     >  2:5604228/1‑140 (MQ=255)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                                                     <  1:1212306/140‑1 (MQ=255)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑AGCACCCCCGGGCGCTTGCGAACAAAATCGCCGCAGCCCGGTGCCCTCGGGGTATCCCTTACGGCGACCGGGGCGGGcgcgg                                                                     >  2:5053252/1‑139 (MQ=14)
                                                                         cATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCCGGCTATGGCCAAAAAAATAGCCGCAGCGCGGGGCCCACGGCATATCCCATAAGGCTACCGGGTCGGGCGCGAGGt                                                                  >  2:7717648/1‑140 (MQ=14)
                                                                           tataATCACTGGCGCAAGGAGCGCGCCGGGGGCCGCCAAACGCCGCCCCCCCC‑AGCACCCCCCGGCTAAGGCGAACCAAATAGCCGCAGCGCCGTGCCCACCGCGTTAACCATACGGGAACCCGGACGGGCGCGAGGTaa                                                                >  2:9227535/1‑140 (MQ=2)
                                                                           tataATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                                <  1:656888/140‑1 (MQ=37)
                                                                             taATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                                              <  1:4754967/140‑1 (MQ=35)
                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                             <  2:3914946/140‑1 (MQ=34)
                                                                              aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCAACCCCGGGCTCTGGCGAACAAAAACGCCCCTGCGCCGTGCCCTCCGCTTATCCCGTACGGCAACCCGGTCGGGCCCGCGGTAACaa                                                             >  2:9838476/1‑139 (MQ=11)
                                                                               aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                                            <  1:8892645/140‑1 (MQ=33)
                                                                               aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                                            <  1:5037331/140‑1 (MQ=33)
                                                                                tCACTGGCGCAAGGAGCGCGCATGGGGCGGCCAAAAGCCGCACCCCCC‑AGCACCCCCCGGCACAGGCGAACAAAATAGCCGCTGCGCGGTGCCCACGGCATAAACCATACGGCTACCGCGTCGGGCGCGACGTAACATcc                                                           >  2:6887261/1‑140 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTATACCATACGGCAACCCGGTCGGGCGCGAGGTAACAAcc                                                           >  2:7873455/1‑140 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAAGCCCCCCCCCCC‑AGCCCCCCCCCGCACTGGCGCACAAAATACCCCCGGCGCGGGGGCCACCGCATATCCCAAAACGCAACCCGGTCCGGCGCGCGGTAACAAcc                                                           >  2:4816052/1‑140 (MQ=2)
                                                                                tCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCCCCCCCCCC‑AGCACCCCCCGGCGCTGGCGAACAAAAAACCCGCGGCGCGGGGCCCAAGGCTTATCCCATAAGGCTACCCGGTCGGGCGCGAGGTAAccacc                                                           >  2:11343101/1‑136 (MQ=11)
                                                                                tCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCCCCGCCCCC‑AGCACCCCCCGGCTCAGGCGAACAAAAAAGCCGCGGCGCGGGGCCCGCGGCTTATCCCATAAGGCGACCCGGTAGGGCGCGAGGTAACAAcc                                                           >  2:6219559/1‑140 (MQ=11)
                                                                                    tGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt                                                       <  1:4686689/140‑1 (MQ=32)
                                                                                     ggCGCAAGGAGCGCGCAGGGGGCGGCCAAACGCCGCCGCCCCC‑AGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCGGCGCGGTGCCCGCGGCTTATACCTTACGGCGACCCGGGCGGGCGCGCGGGAACATCCCTgga                                                      >  2:7672511/1‑138 (MQ=11)
                                                                                         cAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGACCCCCCC‑AGCAACCCCCGGCACTAGCGAACAAAATAGCCCCTGCGCGGTGCCCACCGCTTAACCCATAAGGCTAACGCGTCCGGCGCGAGGTAACATCCCATTAAAAc                                                  >  2:903414/1‑140 (MQ=2)
                                                                                          aaGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                                 <  1:1935014/140‑1 (MQ=32)
                                                                                           aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                                <  1:2459617/140‑1 (MQ=32)
                                                                                           aGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc                                                <  2:8642917/140‑1 (MQ=32)
                                                                                              aGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                             <  2:9094999/140‑1 (MQ=32)
                                                                                              aGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                                             <  2:4503204/140‑1 (MQ=32)
                                                                                                cgcgcCGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                           <  1:4816053/140‑1 (MQ=17)
                                                                                                cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                           <  1:3373015/140‑1 (MQ=32)
                                                                                                cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                           <  2:3512714/140‑1 (MQ=32)
                                                                                                cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                           <  2:2804687/140‑1 (MQ=32)
                                                                                                cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                           <  2:6135614/140‑1 (MQ=32)
                                                                                                 gcgcAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc                                          <  2:287789/140‑1 (MQ=32)
                                                                                                  cgcAGGGGGCGGCCACTCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGGGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCa                                         <  1:3285556/140‑1 (MQ=12)
                                                                                                      gggggCGGCCTATCGCCGCCGCCCCC‑TGCACCCCCGGGCTGTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc                                     <  1:1804471/140‑1 (MQ=17)
                                                                                                         ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                  <  2:5862039/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                  <  1:6219560/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                  <  1:9305293/140‑1 (MQ=32)
                                                                                                         ggcggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                                  <  1:9962259/140‑1 (MQ=32)
                                                                                                            ggcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc                               >  2:10910540/1‑140 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                              <  1:7405965/140‑1 (MQ=32)
                                                                                                             gcCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa                              <  1:8827477/140‑1 (MQ=32)
                                                                                                              ccAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCGTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                             >  1:6733382/1‑140 (MQ=17)
                                                                                                                 aTCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                          >  2:9721659/1‑140 (MQ=32)
                                                                                                                   cgccgccgcCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTGATCCCTTACGGCTCCCGGGTCGGGCGCGAGGTAACCTCCCTGTAAATCGCGCCCTCAGCCCACAGCCATGTggg                        >  2:1731802/1‑140 (MQ=11)
                                                                                                                   cgccgccgcCCCC‑GGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg                        <  2:8311361/140‑1 (MQ=17)
                                                                                                                      cgccgccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTg                     >  1:10751714/1‑140 (MQ=32)
                                                                                                                        ccgccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTATATGGCGCCGTCGGCCCACTTCCATGTGGGCGGcc                   >  2:11618378/1‑140 (MQ=11)
                                                                                                                        ccgccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc                   <  1:7162617/140‑1 (MQ=32)
                                                                                                                        ccgccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc                   >  1:5569855/1‑140 (MQ=32)
                                                                                                                         cgccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGccc                  <  1:5635849/140‑1 (MQ=32)
                                                                                                                          gccCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc                 <  1:10042595/140‑1 (MQ=32)
                                                                                                                             ccc‑TGCACCCCCGGGCTCTGGCGTACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc              >  2:4144906/1‑140 (MQ=17)
                                                                                                                               c‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCt            <  1:3980960/140‑1 (MQ=32)
                                                                                                                                       cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                                       >  1:3345121/1‑74 (MQ=34)
                                                                                                                                       cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGa     >  2:10697599/1‑140 (MQ=32)
                                                                                                                                       cccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGCCTACCCCGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCGTCAGGGa     >  2:10098844/1‑140 (MQ=255)
                                                                                                                                        ccGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGaa    >  1:7802339/1‑140 (MQ=32)
                                                                                                                                         cGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCTGGGAAc   >  2:6693107/1‑140 (MQ=17)
                                                                                                                                          gggCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                                >  1:11395883/1‑78 (MQ=34)
                                                                                                                                          gggCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGACCg  >  2:10310637/1‑140 (MQ=17)
                                                                                                                                          |                                                                                                                                           
TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCC‑TGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTTCAGGGAACG  >  NZ_CP025268/2233111‑2233387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑