Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,414,7050TG20.9% 976.4 / 72.9 460V131V (GTT→GTG) yjfMDUF1190 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (122/242);  new base G (91/5);  total (213/247)
Fisher's exact test for biased strand distribution p-value = 6.10e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 469 total aligned reads displayed.

ACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCA  >  NZ_CP025268/4414566‑4414844
                                                                                                                                           |                                                                                                                                              
   agtGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCt                                                                                                                                             <  2:6561710/138‑1 (MQ=255)
    ttGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTg                                                                                                                                            <  1:11414898/140‑1 (MQ=255)
       cgcgcgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑t                                                                                                                                       <  1:2666628/140‑1 (MQ=255)
           cgTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATAGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑ttttt                                                                                                                                   >  2:4759755/1‑140 (MQ=255)
            gTGGCTGGAACAACGCCAAAACGGCATGGTATGCCGATGTTCCGAAGAATCTGACTCAGCAGAACTGTCAGTCTAAGTACGAAAAGTGCAACTACGACAATGTGGAACTGAGTTGGATCCCGGGCGTGTCTGG‑A‑ttggtg                                                                                                                                  >  2:8146490/1‑136 (MQ=255)
              ggCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGtt                                                                                                                                <  1:9427266/140‑1 (MQ=255)
              ggCTGGAACAACGCCAAAACGGCATGTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACACTGTTGAACAGAGTTGGATCCCGGTCGTTTCTTG‑A‑TTTTTGtt                                                                                                                                >  2:1603511/1‑140 (MQ=255)
               gCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGG‑G‑TTTTTGttt                                                                                                                               >  1:1233411/1‑139 (MQ=255)
                  ggAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGc                                                                                                                            <  1:4547437/140‑1 (MQ=255)
                    aacaacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCg                                                                                                                          >  2:10011762/1‑140 (MQ=255)
                       aacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGt                                                                                                                       <  1:10226516/140‑1 (MQ=255)
                       aacGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGt                                                                                                                       <  2:1954999/140‑1 (MQ=255)
                        acGCCAAAACGGCATTTTATGCCGATGTTCCGACGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑T‑TTTTTGTTAAGCCGGGtt                                                                                                                      >  2:6024374/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATCTGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGGTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑A‑TTTTGTTTAAGCCGGGGTa                                                                                                                     >  2:3554119/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGTACTGTCAGTCTAAGTACGAAAATTGCTACTACGACACTGTTGAACAGAGTTGGATCCCGGTCGGTGCTGG‑A‑TTTTTTTTAAGGCGGGttt                                                                                                                     >  2:4814643/1‑139 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTTAGCCGGGTTa                                                                                                                     >  2:5022330/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTa                                                                                                                     <  2:11846/140‑1 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTa                                                                                                                     >  2:10248431/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTa                                                                                                                     <  2:8252222/140‑1 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTa                                                                                                                     <  2:5782834/140‑1 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑T‑TTTTTGTTAAGCCGGGTTa                                                                                                                     >  1:823257/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑G‑TTTTTGTTTAGCCggggtt                                                                                                                     >  1:3386572/1‑137 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑ATTTTTTGTTATACCGGGtt                                                                                                                      >  1:5371019/1‑140 (MQ=255)
                         cGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGAAGAACTGTCAGTCTAAGTACTAAAATTGCTAATACGACAATGTTGAACAGAGTTGGATCACGGTCGTTTATGG‑A‑TTTTTGTTAAGACGGTTTa                                                                                                                     >  2:10106899/1‑140 (MQ=255)
                         cGCCAAAACGGCATTGTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAAGGTTGAACAGAGTTGGATCCCGGTCGTGTCGGG‑A‑TTTGTGGTATGGCGGGGTa                                                                                                                     >  2:1076967/1‑140 (MQ=255)
                          gCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGG‑T‑TTTTTGTTTATCCggggttt                                                                                                                    >  1:2365682/1‑136 (MQ=255)
                          gCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑A‑TTTTTGTTAATCCGGGTTAt                                                                                                                    >  1:6819901/1‑140 (MQ=255)
                               aaCGTCATTTTATGCCGATGTTCCTAATAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTa                                                                                                               <  2:3114610/140‑1 (MQ=255)
                               aaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCTTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTa                                                                                                               <  1:9221896/140‑1 (MQ=255)
                               aaCGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTg                                                                                                                                  <  1:8927907/121‑1 (MQ=255)
                                   gCATTTTATGCCGTTGTTCCGAATTTTATGACTCAGCAGAACTGTCAGTCTAAGTACTAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCAGGTCGTTTCTGT‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGa                                                                                                           <  2:5240433/140‑1 (MQ=255)
                                         tATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTAGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGa                                                                                                     <  2:8007972/140‑1 (MQ=255)
                                           tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATg                                                                                                   <  1:11185132/140‑1 (MQ=255)
                                           ccccGCTGTACCGAACAATAGGCCTCAGCAGAACTCTCCGTAACAGTTCGAAAATTGCTACGACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGTGTTAGTCGTAAAGATCGCGATg                                                                                                   <  2:6457209/138‑1 (MQ=255)
                                              cGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGGGGTT‑TTTTTGTTATACGGGGTT‑TTGGTTAATATCGCGATG‑Agg                                                                                               >  1:2544615/1‑139 (MQ=255)
                                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑G‑TTTTTTTTAAGCCGGGTTATTCGTAAAGATAGCGAAG‑AGCCg                                                                                             >  2:4399663/1‑140 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGG‑A‑TTTTTGTTAATCCGGGTTATTAGTAAAGATAGCGATT‑AGCCgt                                                                                            >  2:5659950/1‑140 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCgt                                                                                            <  2:11429858/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCgt                                                                                            <  2:10475463/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCgt                                                                                            <  2:7809312/140‑1 (MQ=255)
                                                 tGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCgt                                                                                            <  2:9359657/140‑1 (MQ=255)
                                                         agaaTATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑NGCCGTTTGTTtat                                                                                    <  1:10011101/140‑1 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTTTTAAGCCGGGGTATTCGTTAAGATAGCGATG‑AGCCGGTTGTTTATAACa                                                                                >  1:10730173/1‑140 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGG‑A‑TTTTTTTTTATCCGGGTTAGTAGGAAAGATTGCGATG‑AGCCGTTTTTTTTTaaaa                                                                                >  1:372709/1‑138 (MQ=255)
                                                             tatGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGGGG‑A‑TTTTTTTTTATACGGGGGATTAGTTAAGATCGCGAAG‑AGCCGGTTGGTTTTaaaa                                                                                >  1:1088657/1‑138 (MQ=255)
                                                                 aCTCATCAGAACTGTCAGTCTAAGTAAGAATATTTCTACTACTACAATGTTGAACAGAGTTGGATCCAGTTCGTTTCTGG‑A‑TTTTTGTTAAGCCTGGTTATTCTTAAAGATATCTATG‑ATCCGTTTGTTTATAACAgagg                                                                            <  2:670636/140‑1 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGG‑T‑TTTTTGTTTTGCCGGGGTTTTGGTGAAGATAGCGGTGTGGCCTTTTGTTT‑TAAAATcggcggg                                                                        >  1:5841578/1‑139 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTGGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGt                                                                        <  2:10986747/140‑1 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑A‑TTTTTTTTAATCCGGGTTATTAGTAAAGATGGCGGTG‑TGGCGGTTTTTTATTACAGggggggg                                                                        >  1:10337674/1‑139 (MQ=255)
                                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGATTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGt                                                                        <  2:6786864/140‑1 (MQ=255)
                                                                       cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑T‑TTTTTGTTAAGCCGGGTTATTAGTAAATATCGCGATG‑AGACGGTTGTTTATAACAGCGGCGGTTc                                                                      >  2:8628600/1‑140 (MQ=255)
                                                                       cagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTc                                                                      <  2:11102737/140‑1 (MQ=255)
                                                                             tGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCttt                                                                <  2:2453038/140‑1 (MQ=255)
                                                                              gTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCtttt                                                               <  1:5639054/140‑1 (MQ=255)
                                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTGTGG‑A‑TTTTTTTTAATCCGGGTTATTAGTAAATATCGCGATG‑AGGCGGTTTTTTATAACAGCGGCGGTTACTAttttt                                                              >  2:2764171/1‑139 (MQ=255)
                                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTGCTGG‑A‑TTTTTGTTAAGCCGGGGTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACATCGGCGGTTCCTCTTTTg                                                              >  2:8787867/1‑140 (MQ=255)
                                                                                 aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTGTGG‑A‑TTTTTTTTAATCCCGGTTTTTAGGAAATATCGCGATG‑AGCCGTTTGTTTTTAACAGCGGCGGTTACTCtttttcg                                                            >  1:10492989/1‑137 (MQ=255)
                                                                                  gTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCtt                                                           <  1:5014878/140‑1 (MQ=255)
                                                                                    cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAATCCGGGTTATTCTTAAAGTTAGCGAGT‑AGCAGTGTGTTTATAACAGCGGCGGTTACACTTTTTCttag                                                         >  2:7380660/1‑138 (MQ=255)
                                                                                    cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTAGTAAAGATAGCGATG‑ATCCGTTTGTTTATAACAGCGGCGGTTGCTCtttttcctgt                                                         >  2:8486005/1‑134 (MQ=255)
                                                                                    cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTATGG‑G‑TTTTTTTTTAGCCGGGGTATTGGTAAAGATCGCGATG‑AGCCGGTTTTTTATAAAAGCGGCGGGTCCACTTTTGCGTcg                                                         >  1:4070762/1‑140 (MQ=255)
                                                                                      aaGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTcgcg                                                       >  2:4513310/1‑140 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTTG‑A‑TTTTTGTTTAGCCGGGTTTTTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTACTATTTTTCTTAgcgc                                                      >  2:1739603/1‑140 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTTG‑A‑TTTTTGTTAAGCCGGGGTTTTCGGAAAGATCGCGATG‑AGCCCTTTGTTTATAACAGCGGCGGTTACTAATTTGCTTCgcgc                                                      >  2:3308910/1‑140 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGt                                                                        <  1:7985002/122‑1 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAATATAGCGATG‑ATCCGGTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCgcgc                                                      >  2:3768986/1‑140 (MQ=255)
                                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTGGGTGG‑A‑TTTTTTTTTAGCCGGGTTATTAGGTAATATCGCGATG‑AGGCGGGTGTTTTTAAAAGCGGCGGTTCGACTTTTTCTTCgcgc                                                      >  1:4752630/1‑140 (MQ=255)
                                                                                        gTACGCAAATTGCTACTCCGACAATGTTGAACATAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGATATTCGTAAAGATCGCGATG‑TGCCGTTTGATTATAACAGCGGCGGGTCCTCTTTTGCTTCGCGcc                                                     <  2:4124655/140‑1 (MQ=255)
                                                                                        gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:8546434/140‑1 (MQ=255)
                                                                                        gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:1503029/140‑1 (MQ=255)
                                                                                        gTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGcc                                                     <  1:7571396/140‑1 (MQ=255)
                                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGGTACACTTTTGCTTCGCGCCCAGTc                                                >  2:6092660/1‑140 (MQ=255)
                                                                                                aTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTgg                                             <  1:7216291/140‑1 (MQ=255)
                                                                                                   gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTTG‑A‑TTTTTGTTAAGCCGGGTTATTAGTAAAGATCGCGATG‑AGCCGGTTGTTTATAACAGCGGCGGTTACTATTTTTCGTGGCGCCCAGTCTGgcgc                                          >  2:3985492/1‑140 (MQ=255)
                                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCgcag                                        <  1:65030/140‑1 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTTTTAAGCCCGGGTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATTACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 >  2:9721175/1‑140 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 <  2:6321263/140‑1 (MQ=255)
                                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCt                                 <  1:7690563/140‑1 (MQ=255)
                                                                                                             cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTg                                <  1:974774/140‑1 (MQ=255)
                                                                                                             cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTg                                <  1:2037206/140‑1 (MQ=255)
                                                                                                              aaTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTTTTTAGCCGGGGTATTAGTAAATATCGCGATG‑AGCCGGTTGTTTATAAAAACGGCGGGTCCACTTTTTGTTCGCGCCCCGTCTGGGGCCGCACTTGTgg                               >  1:6034035/1‑140 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCTCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:7521912/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  1:7340250/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  1:9277371/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:5449630/140‑1 (MQ=255)
                                                                                                                  ttGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGATTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:8938074/140‑1 (MQ=255)
                                                                                                                  tagAACAGAGTTGGATCCCGGTCGTTTCAGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                           <  2:9106248/138‑1 (MQ=255)
                                                                                                                    gAACAGAGTTGGATCCCGGTCGTTTCGGG‑A‑TTTTTTTTAAGCCCGGGTATTCGTAAATATCCCGATT‑ATCCCGTTGTTTATAAAATCCGCCGGTCCACATTTGCTTCGCGCCCCATCGGGCGCCGCACATAAGGTGAATa                         >  1:3094793/1‑140 (MQ=255)
                                                                                                                      aCAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACt                       <  2:7121960/140‑1 (MQ=255)
                                                                                                                          agTTGGATCCCGGTCGTTTATTG‑A‑TTTTTTTTAAGCCGGGTTATTAGTAAAGCACGCGATG‑AGCCGTTTTTTTATAACATCGGCGGGTCCTCATTTTATTCGCGCCCAGTCTGGCGCCGCACTTCGGGTGGTTTCTccgg                   >  2:7261652/1‑138 (MQ=255)
                                                                                                                            ttGGTTCCCGGTCGTTTCTGG‑A‑TTTTTGTTAATGCGGGGTTTTGGTATAGTTAGCGATG‑TGGCGTTTGTTTATAACAGCGGCGGGTGCTCTTTTGGTTCGCGCCCAGTGAGGGGCAGCCCGTATGGGGATTAATCCAGgc                 >  2:2261388/1‑140 (MQ=255)
                                                                                                                            ttGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTGATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGGTCCTCTTTTGCGTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACACCTGgc                 >  2:3052536/1‑140 (MQ=255)
                                                                                                                                    cGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTACTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGt         >  2:11169995/1‑140 (MQ=255)
                                                                                                                                    cGGTCGTGTGTGG‑A‑TTTTTGTTAATCCGGGTTATTAGTAAAGATAGCGATG‑ATCCCTTTGTTTTTAACAACGGCGGGTCCTCTTTTGCTTCGCGCCCAGTCTTGAGCCGCACATCAGGTGTTTAATACAGGCGCTCCGGt         >  2:1409807/1‑140 (MQ=255)
                                                                                                                                    cGGTCGTGTCTGG‑A‑TTTTTGTTAATCCGGGGTATTAGTAAATATAGCGATG‑AGCCGTTTTTTTATAACAGCGGCGGGTACAATTTTGCTTCGCGCCCAGTCTGGCGCAGCAAGTCTGGTGATTACTCCAGGCGCTCCGGt         >  2:10869254/1‑140 (MQ=255)
                                                                                                                                      gTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTc       <  2:10664318/140‑1 (MQ=255)
                                                                                                                                          tttCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGc   <  1:9648818/140‑1 (MQ=255)
                                                                                                                                          tttCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGc   <  2:5105894/140‑1 (MQ=255)
                                                                                                                                           ttCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCa  <  1:1837845/140‑1 (MQ=255)
                                                                                                                                           ttCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCa  <  1:6101469/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
ACATTTGCGCGCGTGGCTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGG‑A‑TTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATG‑AGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCA  >  NZ_CP025268/4414566‑4414844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑