Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4120AC25.4% 260.6 / 22.8 197intergenic (+47/‑149)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (63/84);  new base C (9/41);  total (72/125)
Fisher's exact test for biased strand distribution p-value = 2.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 199 total aligned reads displayed.

CAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGG  >  NZ_CP025268/164273‑164551
                                                                                                                                           |                                                                                                                                           
  aaaaGTGTTGATTGGGCGTTATCCAAAAAATTTCTGGCCGTACGACCGGGAAATTATTGCACCGACGCGACGGACGAAAATTTATTGGTTTGGTTGGTTTTCTCCCTCTCCCTGTGGTTGTGGGCCGGGGTGTGGGCATc                                                                                                                                           <  2:6024615/140‑1 (MQ=255)
     agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCCAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgc                                                                                                                                        >  1:7379370/1‑140 (MQ=255)
        gaTGAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAATTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGATAGGGCCGGGTTGAGGGCATCAgcgcg                                                                                                                                     <  2:6893262/140‑1 (MQ=38)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                     >  1:421307/1‑140 (MQ=255)
        gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                     >  2:4921273/1‑140 (MQ=255)
        caTGAACGGGCGTGCCCCCAAACAGGTCGGGCCGGCCGACCGGGCAAAACCTGCCCGGACGCGACGCACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                                     <  2:10309892/139‑1 (MQ=21)
          tcaaaGGCCGTAAACCCAACCTTTTCTGCCCGGACCACCCGTAAAAGACCCCACCGCCGCGACGGACGAAACACTATTCGTAAGGTCGTTTTCCAACCTCTCCCTGAGGCATATGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                                   <  2:1816622/138‑1 (MQ=255)
              tgggCGTAATCCAAAACTTGCGTGGCCGTACGACCCTGAAAATACTCAACCGACGCGACGTACTAATAATTATTGTTATGGTTGGTTTTCTCCTTCTCCTTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                                               <  2:7171028/139‑1 (MQ=16)
              cgggCGTAACCCCAACCATGGCGGGCCGGACGACCCGGCAACTCCGCCCCCGAGGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCCCCCCCTCCCTGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGtt                                                                                                                               <  2:5469688/139‑1 (MQ=255)
                  cctccccccAACCATCCCTCGCCGGACGACCCGGCAAACACCGCCCCGACGCTCCGCACGAAACAGTAATCGTAAGGTTGGTTTTCTCCCTCCCCCGCTGGGAGAGGGCCGGGGTGAGGGCATCATCGCGCACGGtcacc                                                                                                                           <  2:7457907/134‑1 (MQ=19)
                  cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACgggcgcc                                                                                                                           >  1:6554977/1‑134 (MQ=255)
                  cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcc                                                                                                                           >  2:1042027/1‑135 (MQ=255)
                   gTTATCCCAAACATGTCTGGCCGGACGTCCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGGACGtggagcc                                                                                                                          >  2:8225001/1‑134 (MQ=38)
                   gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                          <  2:7706253/140‑1 (MQ=255)
                   gTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                          <  1:10757488/140‑1 (MQ=255)
                   gTTAACCCAAACTTGTCGGGCGGTACGACCGCGCAAATCCGGCCCCGACCCTACGGACGAAAATGTTTTCTTTAGGTTGTTTTTCTCCCTCACCCTGGGGGCGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTtcaccc                                                                                                                          <  2:8943213/140‑1 (MQ=255)
                    ttATCCCAAACTTGTGGGGGCGGACGCCCGGGAAAATACGCCACCGACGCGTCGGACGAAAATTTTTTGGTTTGGTTGTTTTTCGCCCTCGCCGTGGGGGTGTGGGCGGGGGTGGGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  1:4091803/140‑1 (MQ=255)
                    ttATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:5029559/140‑1 (MQ=255)
                    ttATCCCAAACATTTCTGGCCGGACGACCCGCCAAATAGGGCACCGACGCGACGGACGAAAAAGTATACGTATGGTTGGTTTTCTCCCACTCCCTGGGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:10275313/140‑1 (MQ=38)
                    ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:8406460/140‑1 (MQ=255)
                    ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:7050495/140‑1 (MQ=255)
                    ttAGCCCAACCATGTCTGGCCGGACGCCCCGGCAAATCCTCCCCCGACGCGACGGACGAAAAAGTATGCGTACGGTTGTTTTTCCCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:9379598/140‑1 (MQ=37)
                    ttAGCCCAAACAGGACTGGCCGGACGACCCGGCAAACCCTGCACCGACGCCCCGGACGAACAAGTATACGAAAGTTTGGTTTTCCCCCTCTCCCTGGGGGAGAGGGCGGGGGTGAGGCCCTCAGCGCCCACGTtcaccct                                                                                                                         <  2:6134533/140‑1 (MQ=255)
                    taaTCCAAACCATGTGTGGCCGCACGCCCCGGAAAATACTCCACCGACGCGAGGGACTAAAATGTTTTCGTATGGTTGGTTTTCACCCTCACCCTGGGGGTGTGGGCGGGGGGGTGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  2:2478625/138‑1 (MQ=255)
                      cacccAACCATCTCTGCCCGGCCCACCCGCCAACTCCTGCCCCGACGCGACGGACGAAAAACTATCCGAAAGGTTGGTTTACTCCCTCTCCCTGTGGGATAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTtcaccctca                                                                                                                       <  2:10065731/138‑1 (MQ=255)
                      aTCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgca                                                                                                                       >  1:2942920/1‑140 (MQ=255)
                      aTCCAAAACTTGTGTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACTAAAATTTATTCGTATGGTTGTTTTTCTCCCCCTCCGTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca                                                                                                                       <  1:6776158/140‑1 (MQ=37)
                         caaaaCTTGTCGGGCCGGCCCACCCGGAAAACACTCCACCGCCGCGCCGGACCAAAATGTATTCGTAAGGTTGGTTTACCCCCTCACCCTGTGGGAGTGGGCCGGGGTGCGGCCATCAGCGCGCACGTtcaccctcaccc                                                                                                                    <  1:11237427/138‑1 (MQ=255)
                          cAAACAGGCCCGGCCGTACGCCCCGGCACATACTGCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTtcaccctcacccg                                                                                                                   <  2:4021796/140‑2 (MQ=37)
                           aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGCCGGACGAAAAAGTATTCGAAAGGTTGGTGTTCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTa                                                                                                                  <  2:2068174/140‑1 (MQ=38)
                           aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccacacccac                                                                                                                  >  2:4178025/1‑138 (MQ=255)
                            aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGCACCCTCCCCCTaa                                                                                                                 >  2:11260853/1‑140 (MQ=255)
                               atttCTGGCGGGACGACCCGCAAAATACTGCACCGTTGCTACGGACTAAATTTTTTTCTTAGGTTTGTTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                              <  1:4998889/137‑1 (MQ=255)
                               aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                              <  2:11303699/140‑1 (MQ=255)
                                 ggcgggCCGGACGACCCGGCACCTACTGCACCGACGCGACGGACGAAACAGGATTCGTCAGGTTGGTGTTCTCCCTCTCCCGGTGGGCGAGGGCCGGGGGGGGGGCATCAGCGCGCACGGGCGGCCGCACCCTGAcccgg                                                                                                            >  2:5151435/5‑138 (MQ=255)
                                 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGCGGGCCGGGGTGAGGGCAGCCGCGCGCACGTTCACCCACCCCCAAAACctc                                                                                                            >  2:8990686/1‑140 (MQ=38)
                                 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCCCACCCAAAacccc                                                                                                            >  1:827853/1‑138 (MQ=37)
                                 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACACCCAAAaccac                                                                                                            >  1:3867459/1‑138 (MQ=37)
                                 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCTAACCctc                                                                                                            >  2:9251952/1‑140 (MQ=255)
                                 gTCGGGCCGGCCGCCCCGGCAAATACTGCACCCACGCGAGGGACGAAAATGTATTCGTAAGTTTGGTTTTCGCCCTCTCCGTGTGGTTGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  2:5718077/140‑1 (MQ=255)
                                 atcgggCGGGCCGACCCGCAAAATCCTCCACCGACGCGAGGGACTAAAATTTTTTCTTATGGTTGGTTTACCCCCTCTCCGTGTGGGAGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                            <  1:5494758/136‑1 (MQ=255)
                                   ggggCCGGACGACCCGTCAAATACTGCCCCGACGCGACGGACGAACAATTATTCGTATGTTTGGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                          <  2:2645259/138‑1 (MQ=38)
                                   cTGGCCGGACGACCCGGAAAATACTGCACCGACGCGAGGGAGGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                          <  2:309511/140‑1 (MQ=38)
                                    gggCCGCACGACCCGGCAAATACGGCACCCCCGCGACGGACCAAAAAGTATTCGTACGGTTGTTTTTCGCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                         <  1:10956881/139‑1 (MQ=37)
                                      gCCGGCCCACCCGCAAAATCCTCCCCCCACGCGCCGGAATAAAAATTTTTCTTATGTTTGTTTTTCCCCCACTCCGTGGGTGTGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                       <  1:10872455/140‑1 (MQ=255)
                                        cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGTCACCCGCACCCTAACCCTCTCCCGCa                                                                                                     >  2:6394636/1‑140 (MQ=38)
                                          gactacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:9556331/140‑1 (MQ=255)
                                          gacgccCCGGCAAATCCTGCACCGACGCGACGGCCGAAAACGTATTCGTATGGTTGTTTTTCGCCCTCTCCCGGGGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:5864867/140‑1 (MQ=255)
                                          gacgacCCGGCAAATACTCCACCGACGCGCCGTACGAAAAATTATTCGTAAGTTTGGTTTACTCCCTCACCCTGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  2:5865312/140‑1 (MQ=38)
                                          gacgacCCGGAAAATCCTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGTTGTTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                   <  1:8816661/140‑1 (MQ=38)
                                              ccccGGAAAATACTGCACCTCCGCGACGTACTAAAATGTATTCGTAAGGTTGTTTTTCCCCCTCACCCTGTGGGTGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  1:8930186/139‑1 (MQ=255)
                                              ccccGCCAACTACTCCACCGACGCGCCGGACTAAACAGTATTCGTACGGTGGGTTTTCCCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCCACAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  1:7792762/139‑1 (MQ=37)
                                              acCCGGCAAATACGGCACCGACGCGACGTAAGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCACCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                               <  2:520847/140‑1 (MQ=38)
                                               cccGGAAAATACTGCCCCGACGCGACGGCCGAAAAATTATGCGTAGGGTGGGTTTACTCCCTCACCCTGTGGGAGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga                                                                                              <  1:3888652/140‑1 (MQ=255)
                                                ccGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGagag                                                                                             <  2:10198764/140‑1 (MQ=255)
                                                     aattACTCCACCGCCGCGCGGTACTAAAAATTATTCTTATGTTTGTTTTTCACCCCCCCCTTGTGGTTTCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                        <  1:9056838/137‑1 (MQ=255)
                                                     aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGGTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCCCGGGAAACCGCACCCCAAACCACGCCCTCCAGGGAGGGGGGAc                                                                                        >  2:2939820/1‑140 (MQ=25)
                                                         aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCTCACCCTCTCCCGCACGGGAGTGGGGGCCggt                                                                                    >  2:9106997/1‑138 (MQ=37)
                                                         aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCAAAACCACACCCGCAAGGGGGAGGGGGCCgct                                                                                    >  1:7622857/1‑138 (MQ=37)
                                                         aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCACCAGGGAGCGGGGTCCGaa                                                                                    >  2:9424437/1‑139 (MQ=37)
                                                          cTGCACCGACGCGACGGACGTAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGGTCCCCCTCACCCAAACCCTCTCCCTCAAGGGCGAGGGGACCGagc                                                                                   >  2:7764670/1‑138 (MQ=37)
                                                             cACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCTCACCCTAAACCACTCCCTCTAGGGTGAGGGGACCGATagag                                                                                >  2:1907864/1‑140 (MQ=37)
                                                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCCCCCAAAACCACACCCACAACGGGGAGGGGGcccaaagag                                                                                >  2:2247674/1‑133 (MQ=21)
                                                             cACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCAGCCGCGCGCACGTTCACCCGCACCCGAACCCTCTCCCTCAAGGGCGCGGGGACCGAGCgcg                                                                                >  2:10684986/1‑138 (MQ=37)
                                                                cgacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGACCCCCACCCAAACCCACTACCACCCGGGAGGGGGGGCCGCTCGGGccc                                                                             >  2:4312453/1‑138 (MQ=21)
                                                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCGAACCCACACCCACAAGGGGGAGGGGACCCATCGAGCACaa                                                                           >  2:4723994/1‑140 (MQ=37)
                                                                     cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtt                                                                        >  2:8559451/1‑140 (MQ=255)
                                                                       cctgacgAAAAATTATTCTGATGGTTGGTTTTCGCCCTCTCCGTGTGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                      <  1:173692/137‑1 (MQ=37)
                                                                       acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCAACCGCACCCGAAACCTCTCCCACAAGGGAGATGGGACCGATCGAGGCCAAAtttt                                                                      >  2:1655457/1‑140 (MQ=37)
                                                                            cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                                 <  2:10994185/140‑1 (MQ=255)
                                                                               caatgTATTGGTACGGTGGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGata                                                              <  1:151059/136‑1 (MQ=38)
                                                                                acaGTATTCGTCCGGTTGGTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGagat                                                             <  2:1559684/138‑1 (MQ=38)
                                                                                aatgTATCCTTACGGTTGGTTTACCCCCTCTCCCTGGGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                                             <  1:5458394/137‑1 (MQ=38)
                                                                                 aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc                                                            <  2:2595759/140‑1 (MQ=255)
                                                                                     aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCCCAAAGGAGCGGGGACCCCACGCGCACAAAAATTGTGAGataaaataa                                                        >  1:9624178/1‑134 (MQ=18)
                                                                                      ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                       <  2:6319513/140‑1 (MQ=255)
                                                                                      tacGTAAGGTGGGTTTACGCCCTCTCCCTGTGGGAGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                                       <  1:10309565/138‑1 (MQ=39)
                                                                                       tcttAAGGTGGTTTGTCTCCCTATCCCTGTGGGATTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  1:4610587/137‑1 (MQ=38)
                                                                                       tCGTATGTTTGGTTTTCTCCCTCGCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  1:10494345/140‑1 (MQ=255)
                                                                                       tCGTATGTTTGGTTTTCTCCCACTCCCGGTGGGTGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  1:11414870/140‑1 (MQ=39)
                                                                                       tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  2:6582484/140‑1 (MQ=255)
                                                                                       gcGTATGGTTGGTTTCCCCCCTCTCCCTGGGGGTGAGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                                      <  1:190200/139‑1 (MQ=38)
                                                                                         ttaggggtggttTTCGCCCGCTCCGTGGGGGCGTGGGCCGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                    <  1:4520079/133‑1 (MQ=37)
                                                                                         gTAAGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                                    <  2:7518157/140‑1 (MQ=255)
                                                                                            aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                                 <  2:10362651/140‑1 (MQ=255)
                                                                                                  gttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAACGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTATGTCtttttaa                                           >  2:7941227/1‑135 (MQ=25)
                                                                                                       ctccctcgccctGGGGGAGAGGGCCGGGGTGCGGCCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag                                      <  1:8657591/140‑1 (MQ=39)
                                                                                                            tctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCACCAGGGAGAGGGGGCCGCTCGAGCCCCAATTTTGTGAGATATATTATTCTGTAGTGTAAAAGAATAACac                                 >  2:1756852/1‑139 (MQ=25)
                                                                                                                    ggggcgtgggCGGGGGGGCGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg                         <  1:2490347/133‑1 (MQ=38)
                                                                                                                        agagGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCGAAACCTATCCCACCAGGGTGAGGGGACCGCTCGAGCCCAAAATTTGTGAGATATATTATTAAGTGTTGTTACAGAAGGACAGGAAAACGGGcgt                     >  2:7922881/1‑138 (MQ=255)
                                                                                                                        agagGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                         <  1:9410773/40‑1 (MQ=255)
                                                                                                                        agagGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCTCCCCCAAAACCACACCCACAATGGTGAGGGGTCCCCGAGAGCACCAAATTTTTTAGATATATTTATATGTGTTGTTAAAAAAGAAAAGAAAATAgggggt                     >  2:2301036/1‑137 (MQ=255)
                                                                                                                         gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                    <  2:10315740/140‑1 (MQ=255)
                                                                                                                          agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCTATACCACAAGGGGGAGGGGTCCGATGGATCACAAATTTTTTTATATATAATATTATGTATTTTTACAGAATAAAAGAAAATAGGGCgttt                   >  2:11513070/1‑140 (MQ=255)
                                                                                                                            ggCCGGGGTGAGGGCATCAGCGCGCACGGGGACCCACACCCTAACCCACTCCCGCAAGGGCGGGGGGACCCATCGCGCACAAATTTTGAGAGCTATCTTATTGTGTGTGGGAACCGAAGAACAGCAAATAGGGCGTTTgc                 >  2:8837982/1‑140 (MQ=255)
                                                                                                                              ccGGGGTGCGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTTAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc               <  2:8374155/140‑1 (MQ=255)
                                                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTTGACCCCCCCCCGAACCCTCTACCGCCAGGGCGAGGGGACCCCTCGCGCGCCAAATTTGGGAGAAAAAATCATCTGTCTTGTTACAGAAGAACAGAAAATCTGGCtttttagc               >  2:11448207/1‑136 (MQ=255)
                                                                                                                                ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt             <  1:4793181/140‑1 (MQ=255)
                                                                                                                                ggggTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt             <  2:3867458/140‑1 (MQ=255)
                                                                                                                                     gAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata        <  1:6620488/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGG  >  NZ_CP025268/164273‑164551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑