Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 49,832 C→T S3S (AGC→AGT)  folA → type 3 dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP02526849,8320CT99.6% 1929.4 / NA 508S3S (AGC→AGT) folAtype 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (266/238);  minor base A (0/2);  total (268/240)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 508 total aligned reads displayed.

CGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAAT  >  NZ_CP025268/49695‑49968
                                                                                                                                         |                                                                                                                                        
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                                                                                                                                      >  1:8580508/1‑140 (MQ=255)
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                                                                                                                                      >  2:6571427/1‑140 (MQ=255)
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                                                                                                                                      >  1:5893336/1‑140 (MQ=255)
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                                                                                                                                      >  2:7272069/1‑140 (MQ=255)
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACAACATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGa                                                                                                                                      >  2:7173180/1‑140 (MQ=255)
  aGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAAGATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACCATTTTTTTTATCGGGACCTCTCAAGGATCAGTCTGa                                                                                                                                      >  2:3670292/1‑140 (MQ=255)
    tCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGATTACGCGTTACGTATAGTTGCGACAATTTTTTTTATCGGGAAATCTCCATGATAAGTCTGagt                                                                                                                                    >  2:4391405/1‑138 (MQ=255)
    tCCAGGGAGAGAGCGTGGAATCGCCAGCAGAATATAAAATTTTCCTCAACATAATCCTAGCACCATTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATAGGGAAATCTCAATGATCAGTCTGAtt                                                                                                                                    >  1:1298695/1‑140 (MQ=255)
      cAGGGAGAGAGCCTGGACTCGCCAGCAGAATATACAATGGTCCTCAACCTCCTCCACGCACCAGTCGACGACGGTTTACGCTGTACGTATAGTGGCGACACTTTTTTTTATCGGGAAAGCTCAAGGCTCAGTCTGATTgc                                                                                                                                  <  2:9215044/140‑1 (MQ=255)
       aGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCACCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                                                                                                 <  2:9838609/140‑1 (MQ=255)
       aGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcg                                                                                                                                 <  2:2382935/140‑1 (MQ=255)
           agagcgCGTGGACTGGCCAGCAGAATATCTCATGTTCCTCCACCTCCTCGTCGCCCCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAATTTTTTTATCGGGAAATCACACTGATCAGACTCTTTGCGGCGt                                                                                                                             <  2:3834067/140‑1 (MQ=255)
           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                                                                                             <  1:1110300/140‑1 (MQ=255)
           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                                                                                             <  2:5258891/140‑1 (MQ=255)
           agagagCGTGGACTCGCCAGCACAATATAAAATTGTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                                                                                             <  1:9127829/140‑1 (MQ=255)
             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGttt                                                                                                                           >  1:2453524/1‑139 (MQ=255)
             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa                                                                                                                           >  2:5538652/1‑140 (MQ=255)
              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                                                                                          >  1:231266/1‑140 (MQ=255)
              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                                                                                          >  2:11290786/1‑140 (MQ=255)
              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                                                                                          >  2:10090609/1‑140 (MQ=255)
              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                                                                                          >  1:4322516/1‑140 (MQ=255)
              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                                                                                          >  1:5399630/1‑140 (MQ=255)
               agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                                                                                         >  2:8362180/1‑140 (MQ=255)
               agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGc                                                                                                                         >  1:678469/1‑140 (MQ=255)
                   tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTa                                                                                                                     >  2:6431704/1‑140 (MQ=255)
                   tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTa                                                                                                                     >  1:5528464/1‑140 (MQ=255)
                   tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAGTGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAGCAGTCTGATTGCGGCGTTAGCGGtc                                                                                                                     >  2:10949211/1‑139 (MQ=255)
                    ggACTCGCCAGCAGAATATAAAATTTTCCTCCCCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAg                                                                                                                    <  1:8358769/140‑1 (MQ=255)
                          gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATcgcg                                                                                                              >  1:9330025/1‑140 (MQ=255)
                             atcagAATATAAAAATTTCCTCCACATCATCCTCGCTCCAGTCGACGACGGTTTACGCTTTACGGATAGTGTCGTCTATTTATTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTa                                                                                                           <  2:7559203/138‑1 (MQ=255)
                              gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                                                                                          <  2:3353826/140‑1 (MQ=255)
                                   atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa                                                                                                     <  1:1508682/140‑1 (MQ=255)
                                   atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa                                                                                                     <  2:9088952/140‑1 (MQ=255)
                                    tataAAATTTTCCTCCACATCATCCTCGCACCAGTAGACGACGGTTTACGATTTAAGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCTGTCTGATTGCGGCGTTAGCTGTAGATCGCGTTATCGGCAt                                                                                                    <  2:2116844/140‑1 (MQ=255)
                                    tataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt                                                                                                    >  1:10698180/1‑140 (MQ=255)
                                    tataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt                                                                                                    >  2:10715176/1‑140 (MQ=255)
                                       aaaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGa                                                                                                 <  2:1584851/140‑1 (MQ=255)
                                         aaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaaa                                                                                               >  1:3716547/1‑140 (MQ=255)
                                            ttgccTCAACCTCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                                                                                            <  2:8581176/137‑1 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  2:9506409/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  1:7913630/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  2:3077273/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  1:6465492/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  2:6753282/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  1:9812473/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  1:3898083/1‑140 (MQ=255)
                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAGCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                          >  2:2942021/1‑140 (MQ=255)
                                               ccTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCa                                                                                         <  2:2697612/140‑1 (MQ=255)
                                               ccTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCa                                                                                         <  2:7050856/140‑1 (MQ=255)
                                                cTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt                                                                                        <  2:1474413/140‑1 (MQ=255)
                                                    aCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg                                                                                    <  2:10302202/140‑1 (MQ=255)
                                                          tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                              >  1:5999257/1‑140 (MQ=255)
                                                          tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                              <  1:5059871/140‑1 (MQ=255)
                                                          tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAc                                                                              >  2:8081567/1‑140 (MQ=255)
                                                            cTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcct                                                                            <  2:7798959/140‑1 (MQ=255)
                                                                cACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                        >  1:9414338/1‑140 (MQ=255)
                                                                    aGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCATTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCg                                                                    <  2:5416154/140‑1 (MQ=255)
                                                                    aGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCg                                                                    >  1:2651790/1‑140 (MQ=255)
                                                                     gtcgacgacGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAATCGCCATGCCGTGGAACCTGCCTGCCGa                                                                   >  1:5447048/4‑140 (MQ=255)
                                                                     gtcgacgacGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGa                                                                   >  1:3549174/4‑140 (MQ=255)
                                                                      tcgacgacGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAt                                                                  >  2:5141519/3‑140 (MQ=255)
                                                                           gacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                                             <  1:4742541/140‑1 (MQ=255)
                                                                           gacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                                             <  1:2002349/140‑1 (MQ=255)
                                                                            acgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGcc                                                            <  2:7432606/140‑1 (MQ=255)
                                                                             cgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGccc                                                           <  2:8823547/140‑2 (MQ=255)
                                                                             cgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGccc                                                           >  1:9591602/1‑139 (MQ=255)
                                                                             cgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGccc                                                           <  2:116235/140‑2 (MQ=255)
                                                                                tttACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTNGAACCTGCCTGCCGATCTCGCCCGGt                                                        >  1:7118631/1‑140 (MQ=255)
                                                                                tttACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGt                                                        >  1:5319909/1‑140 (MQ=255)
                                                                                    cGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCCATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTaa                                                    <  1:10531756/140‑1 (MQ=255)
                                                                                        ttACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGc                                                >  1:1083013/1‑140 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               <  1:3354458/140‑1 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               <  1:11230185/140‑1 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               <  2:4555544/140‑1 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               <  2:4807729/140‑1 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               >  1:3014358/1‑140 (MQ=255)
                                                                                         tACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCa                                               <  1:5791717/140‑1 (MQ=255)
                                                                                          aCGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCaa                                              <  1:4057316/140‑1 (MQ=255)
                                                                                          aCGTATAGTGGCGACAATTTTTTTGATCGGGAAATCTCAATGATCAGTCGGATGGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGCAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCaa                                              <  2:1360896/140‑1 (MQ=255)
                                                                                              ataGTGGCGACAATTTTTTTTATCGGGAAATCTCCAGGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACAcc                                          >  2:6093961/1‑140 (MQ=255)
                                                                                                 gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTta                                       >  1:4163019/1‑140 (MQ=255)
                                                                                                 gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTta                                       >  1:8317273/1‑140 (MQ=255)
                                                                                                 gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTta                                       >  1:9689553/1‑140 (MQ=255)
                                                                                                   ggCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                                                                                             <  1:7739007/52‑1 (MQ=255)
                                                                                                     cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaata                                   >  2:8925513/1‑140 (MQ=255)
                                                                                                     cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaata                                   >  1:8041826/1‑140 (MQ=255)
                                                                                                     cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaata                                   >  1:460022/1‑140 (MQ=255)
                                                                                                       aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataaa                                 >  1:10351524/1‑140 (MQ=255)
                                                                                                       aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataaa                                 >  1:698692/1‑140 (MQ=255)
                                                                                                       aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTtaaataaa                                 <  2:5705922/140‑1 (MQ=255)
                                                                                                                   aTCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                     <  2:10994856/140‑1 (MQ=255)
                                                                                                                    tCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATgg                    <  2:4163018/140‑1 (MQ=255)
                                                                                                                        gAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                <  1:1639604/140‑1 (MQ=255)
                                                                                                                        gAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                <  1:7257762/140‑1 (MQ=255)
                                                                                                                          aaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc              <  1:10204088/140‑1 (MQ=255)
                                                                                                                          aaTCTCAATGATCAGTCTGAGTGCGGCGTTAGCGGGAGATCGCGTTATCGGCATGGAAAACTCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc              <  2:8751535/140‑1 (MQ=255)
                                                                                                                               cAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGACCCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATAcc         <  1:2818310/140‑1 (MQ=255)
                                                                                                                                aaTGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt        >  1:6342993/1‑140 (MQ=255)
                                                                                                                                    aTCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa    <  1:8844192/140‑1 (MQ=255)
                                                                                                                                      cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCCGGTTTAAACGCAACACCTTAAATAAACCCGGGATTATGGGCCGCCATACCTGGGaat  >  1:6123166/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAAT  >  NZ_CP025268/49695‑49968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑