BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	550,382	A→G	298 (TAA→TAG)	ybcF →	carbamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	550,382	0	A	G	99.7%	2513.9 / NA	720	298 (TAA→TAG)	ybcF	carbamate kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (278/440); minor base T (0/2); total (278/442)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 721 total aligned reads displayed.

GCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTA  >  NZ_CP025268/550245‑550518

 ccGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCg                                                                                                                                       <  1:8123934/140‑1 (MQ=255)

  cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  2:2569698/140‑1 (MQ=255)

  cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      >  1:3336803/1‑140 (MQ=255)

    aTGGTTCGAGGGGGCCGACTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTATACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAg                                                                                                                                    <  2:6266307/140‑1 (MQ=255)

     tGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAgg                                                                                                                                   <  2:3998427/140‑1 (MQ=255)

          cGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTa                                                                                                                              <  1:6226218/140‑1 (MQ=255)

           gATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAg                                                                                                                             >  1:10562016/1‑140 (MQ=255)

                ggCCGAATGTAACGGCGGTGAGTGGTTATGTCAGATGCCGTGGTAAACCCGCGTGGAGTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAtt                                                                                                                        <  2:10651634/140‑1 (MQ=255)

                  ccGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTtg                                                                                                                      >  2:5527794/1‑140 (MQ=255)

                  ccGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTtg                                                                                                                      >  1:1976243/1‑140 (MQ=255)

                      aTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGcc                                                                                                                  <  2:5983101/140‑1 (MQ=255)

                       tGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCt                                                                                                                 >  1:8637861/1‑140 (MQ=255)

                          aaCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                              >  1:2120336/1‑140 (MQ=255)

                          aaCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                              >  1:5259906/1‑140 (MQ=255)

                           aCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                             >  1:1375748/1‑140 (MQ=255)

                            cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                            <  2:8777786/140‑1 (MQ=255)

                               cggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGAc                                                                                                         >  1:6771917/1‑140 (MQ=255)

                                ggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACg                                                                                                        >  2:449929/1‑140 (MQ=255)

                                     gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  1:9486760/140‑1 (MQ=255)

                                     gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  2:10358995/140‑1 (MQ=255)

                                     gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   >  2:4716710/1‑140 (MQ=255)

                                         ttATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGAcgcg                                                                                               >  2:5005695/1‑140 (MQ=255)

                                              tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTa                                                                                          >  1:1772279/1‑140 (MQ=255)

                                              tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTa                                                                                          >  1:4640175/1‑140 (MQ=255)

                                                aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  1:1122848/140‑1 (MQ=255)

                                                aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  1:10444498/140‑1 (MQ=255)

                                                aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  1:10143629/140‑1 (MQ=255)

                                                aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTNGACGCGTCTTATc                                                                                        <  1:10006198/140‑1 (MQ=255)

                                                aGAAGCAGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAATTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:9127220/140‑1 (MQ=255)

                                                 gAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCTGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCGGATGCGCCGCTTGACGCGTCTTATCa                                                                                       <  2:53338/140‑1 (MQ=255)

                                                 gAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                       <  2:10005289/140‑1 (MQ=255)

                                                 gAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                       <  1:5612464/140‑1 (MQ=255)

                                                  aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTATGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  2:6463696/1‑140 (MQ=255)

                                                  aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAg                                                                                                                             >  1:7864414/1‑101 (MQ=255)

                                                  aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  1:11032564/1‑140 (MQ=255)

                                                  aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  1:7660462/1‑140 (MQ=255)

                                                   aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAgg                                                                                     <  2:2879163/140‑1 (MQ=255)

                                                   aGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAgg                                                                                     <  1:2367901/140‑1 (MQ=255)

                                                       gTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTa                                                                                 >  2:10859562/1‑140 (MQ=255)

                                                        tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                                >  1:6860072/1‑140 (MQ=255)

                                                        tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                                >  1:11479761/1‑140 (MQ=255)

                                                          gTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                              >  2:22875/1‑140 (MQ=255)

                                                           tAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAc                                                                             <  1:4917843/140‑1 (MQ=255)

                                                           tAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAc                                                                             <  1:3020172/140‑1 (MQ=255)

                                                           tAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAc                                                                             <  1:4296465/140‑1 (MQ=255)

                                                            aaaCCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAcc                                                                            <  2:5778507/140‑1 (MQ=255)

                                                             aaCCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCNAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCg                                                                           >  1:10784124/1‑140 (MQ=255)

                                                                ccGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTg                                                                        >  1:3997915/1‑140 (MQ=255)

                                                                   cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCg                                                                     <  1:8869810/140‑1 (MQ=255)

                                                                    gTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGc                                                                    >  1:10304150/1‑140 (MQ=255)

                                                                    gTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGc                                                                    >  2:11403662/1‑140 (MQ=255)

                                                                      ggATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCAt                                                                  <  2:8430045/140‑1 (MQ=255)

                                                                      ggATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCAt                                                                  <  2:3316891/140‑1 (MQ=255)

                                                                         ttGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCg                                                               >  2:7974910/1‑140 (MQ=255)

                                                                              gCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAt                                                          <  2:6925743/140‑1 (MQ=255)

                                                                                  tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGGTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGt                                                      >  2:11143026/1‑140 (MQ=255)

                                                                                  tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGt                                                      <  2:6103599/140‑1 (MQ=255)

                                                                                      gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                  <  2:6833033/140‑1 (MQ=255)

                                                                                      gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                  >  2:2761648/1‑140 (MQ=255)

                                                                                      gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                  <  1:8041062/140‑1 (MQ=255)

                                                                                       cgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcaca                                                 <  2:8214885/140‑1 (MQ=255)

                                                                                       cgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcaca                                                 <  1:3528457/140‑1 (MQ=255)

                                                                                       agAATTGACGAGACGCTGGCGGGCGAAGCGGGGACCTGTAGTTCGCGGTAGTCGTAGGCATTAGACAGTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcaca                                                 <  2:1246923/139‑1 (MQ=38)

                                                                                        gAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACaa                                                <  2:4760002/140‑1 (MQ=255)

                                                                                         aaTTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAAt                                               >  1:5814199/1‑140 (MQ=255)

                                                                                           ttGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGc                                             >  2:1011946/1‑140 (MQ=255)

                                                                                              aaGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTg                                          >  1:9201808/1‑140 (MQ=255)

                                                                                                 agaCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg                                       <  1:4191269/140‑1 (MQ=255)

                                                                                                   aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcg                                     >  2:7800977/1‑140 (MQ=255)

                                                                                                      cTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAATTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcgatg                                  <  1:8248874/140‑1 (MQ=255)

                                                                                                        ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCNGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCt                                >  1:6307390/1‑140 (MQ=255)

                                                                                                           gggcgTAGCGGGGACCGGTAGTTCGCTATAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCGTGACGCGTCTTATCAGGCCTACAACCGGGGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  2:7299973/140‑1 (MQ=38)

                                                                                                           gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  2:4254376/140‑1 (MQ=255)

                                                                                                           gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  1:9700767/140‑1 (MQ=255)

                                                                                                           gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  1:7383350/140‑1 (MQ=255)

                                                                                                           gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  1:5440491/140‑1 (MQ=255)

                                                                                                           gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  2:1834582/140‑1 (MQ=255)

                                                                                                             gcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACg                           >  1:3728908/1‑140 (MQ=255)

                                                                                                              cgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGc                          <  1:1617198/140‑1 (MQ=255)

                                                                                                              cgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGc                          <  1:8548353/140‑1 (MQ=255)

                                                                                                               gAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCa                         <  1:2612416/140‑1 (MQ=255)

                                                                                                                     gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGCTGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAAGTGGTGCACCATGCCTGATGCGATGCTTGACGCCTCTTAt                   <  2:3093114/140‑1 (MQ=39)

                                                                                                                     gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  1:5098549/140‑1 (MQ=255)

                                                                                                                     gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:9331997/140‑1 (MQ=255)

                                                                                                                     gggACCTGTAGTTCGCTGTAGTCGTAGGCATGAGACATTTGTGCCTGATGCGACGCTGGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:2041981/140‑1 (MQ=255)

                                                                                                                      ggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATc                  <  1:6516390/140‑1 (MQ=255)

                                                                                                                       gACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCa                 >  2:9546361/1‑140 (MQ=255)

                                                                                                                         ccTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTGCAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAgg               <  2:4930013/140‑1 (MQ=255)

                                                                                                                           tGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGcc             >  1:1261235/1‑140 (MQ=255)

                                                                                                                           tGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGcc             >  1:2801739/1‑140 (MQ=255)

                                                                                                                            gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt            <  2:7144029/140‑1 (MQ=255)

                                                                                                                               tttCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACa         <  2:3813116/140‑1 (MQ=255)

                                                                                                                               tttCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACa         <  2:9815136/140‑1 (MQ=255)

                                                                                                                                 tCGCTGTATTCGTAGGCATTATACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  2:3624419/140‑1 (MQ=255)

                                                                                                                                 tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  2:10539133/140‑1 (MQ=255)

                                                                                                                                 tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:7128565/140‑1 (MQ=255)

                                                                                                                                 tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCATACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  2:1457403/140‑1 (MQ=255)

                                                                                                                                    cTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATggg    >  2:10994878/1‑140 (MQ=255)

                                                                                                                                    cTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATggg    >  2:11588907/1‑140 (MQ=255)

                                                                                                                                     tGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  2:9723475/114‑1 (MQ=255)

GCCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTA  >  NZ_CP025268/550245‑550518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`