BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,201	T→C	pseudogene (667/667 nt)	CXP41_RS03085 ←	class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,201	0	T	C	99.4%	2670.3 / NA	774	pseudogene (667/667 nt)	CXP41_RS03085	class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (446/323); minor base A (1/4); total (447/327)
Fisher's exact test for biased strand distribution p-value = 1.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 774 total aligned reads displayed.

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGT  >  NZ_CP025268/581069‑581337

atatCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTAtttt                                                                                                                                    >  2:4645189/1‑140 (MQ=255)

       gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTg                                                                                                                             <  1:9275231/140‑1 (MQ=255)

            gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCg                                                                                                                        <  1:3315836/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCCCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  2:8985927/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGTCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  2:3511664/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                     <  1:1536358/140‑1 (MQ=255)

               gAACCGTGTTGATACATCAACTGCACCTGATAGTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCTAGAACATGCGGC‑GGT‑TGGATGCTATTTGTTTCCTGAAGCGGaa                                                                                                                     <  2:6947967/140‑1 (MQ=255)

                aaCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaa                                                                                                                    >  2:9543252/1‑140 (MQ=255)

                 aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                   <  1:8679258/140‑1 (MQ=255)

                 aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                   <  1:9146005/140‑1 (MQ=255)

                 aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                                   >  1:10054835/1‑140 (MQ=255)

                  ccGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAAc                                                                                                                  >  2:7533401/1‑140 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                <  1:9663529/140‑1 (MQ=255)

                    gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                                <  1:3631358/140‑1 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  1:8241264/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  1:3828494/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  2:9095389/1‑140 (MQ=255)

                     tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                               >  1:10231231/1‑140 (MQ=255)

                     tgatGATACATCAACTGCACCTGAAATTGAGTGGCCTACGAACCCAGACAGGGAGTAATCATTGGGATTATGACGCAGCACGTATTAAGCAAGAACATGCTGC‑GCT‑TCGATCCTATTTATTACCTGAAGCCGAAAAAAta                                                                                                               >  2:7317163/4‑139 (MQ=255)

                      gtTGATACATCTACTGCACCTGATATTGAGGGGCCTACGAACCCTGTCAGTGAGCAATCATTGGGAATATGCCGCAGCACGACAGCAGCACGCCCATCCTGCGGGT‑G‑GACGCTAATTGGTTCCCGAAGCGGAAAACATta                                                                                                              <  2:2746341/140‑1 (MQ=255)

                      gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                              <  1:2036950/140‑1 (MQ=255)

                      gtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                              <  1:11211693/140‑1 (MQ=255)

                       ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                                             >  1:2273492/1‑140 (MQ=255)

                       ttGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtac                                                                                                             >  1:10428234/1‑140 (MQ=255)

                         gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                           <  1:4700262/140‑1 (MQ=255)

                          aTACATCAACTGCACCTGATATTGAGTGGCCTACCTACCCTGTCAGGGAGTAATCATTGGGTTTATGCCGCAGCACGTCTTAAGCAAGTACATGCTGC‑GGT‑TGGATGCTAATTTTTTCCTTAAGCGGAAAACATtactac                                                                                                          <  2:1710694/140‑1 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                         <  2:130175/140‑1 (MQ=255)

                           tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                         <  1:4968545/140‑1 (MQ=255)

                            aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGGG‑G‑GATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                        >  2:608077/1‑140 (MQ=255)

                            aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                        <  1:9815890/140‑1 (MQ=255)

                            aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                        <  1:9989067/140‑1 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTCTTTTTTTACTGAAGCGGAAAACATTACTAAAGTAc                                                                                                     >  2:10557315/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATta                                                                                                              <  1:5662912/131‑1 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  1:6659961/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  2:10806316/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGAATATGCCGCAGCTCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  2:6147997/1‑140 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCAGTGGGATTCTGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGTGG‑GATGCTATTTTTTTCCTTAAGCGGAAAACATTACTACAGttc                                                                                                     >  2:10112879/1‑138 (MQ=255)

                               tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCAGTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                     >  2:5325649/1‑140 (MQ=255)

                                    tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                                >  1:7276464/1‑140 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGTACCCTGTCAGGGAGTAATCTTTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCGATTTTTTTCCTGAAGCGGAAAACATTACTACAGGACCTTGACCCt                                                                                            <  2:4137850/140‑1 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            <  1:4348955/140‑1 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            <  1:2911599/140‑1 (MQ=255)

                                        ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                            >  1:5547686/1‑140 (MQ=255)

                                            atatTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGt                                                                                        >  2:11166231/1‑140 (MQ=255)

                                               ttGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGACGCGGAAAACATTACTACAGTACCTTGAACCTTGGtttt                                                                                     <  2:676915/140‑1 (MQ=255)

                                                tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCTGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                                    <  2:2476057/140‑1 (MQ=255)

                                                tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                                    >  1:8465314/1‑140 (MQ=255)

                                                 gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                   <  2:5598558/140‑1 (MQ=255)

                                                 gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                   <  2:8557078/140‑1 (MQ=255)

                                                  aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                                  >  1:6050318/1‑140 (MQ=255)

                                                   gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                                 >  2:11519120/1‑140 (MQ=255)

                                                    tGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAt                                                                                >  1:7659096/1‑140 (MQ=255)

                                                     ggCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAtt                                                                               >  2:5207233/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTTC‑GGT‑TGGATGCTATTTTTTTCATGAAGCGGAAAACATTACTACAGTAACATGAACCTTGGTTTTAACAtta                                                                              >  2:10336325/1‑139 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                              >  2:11003768/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                              >  1:10919623/1‑140 (MQ=255)

                                                      gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                              >  2:5071499/1‑140 (MQ=255)

                                                       ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                             >  2:824388/1‑140 (MQ=255)

                                                       ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                             >  2:3877517/1‑140 (MQ=255)

                                                           cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                         <  2:4284229/140‑1 (MQ=255)

                                                            gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                        <  1:2530402/140‑1 (MQ=255)

                                                              aCCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATg                                                                      <  2:5707001/140‑1 (MQ=255)

                                                                 cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                                   >  1:6219098/1‑140 (MQ=255)

                                                                  tGTCTGTGAGTAATCATAGGGATTATTCCGCAGCACGTATTAACCAAGAACAATCTGC‑GGT‑TGGATGCTAATTATTACCTGAAGCTGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  <  2:6497561/140‑1 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  <  1:1060379/140‑1 (MQ=255)

                                                                  tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  <  1:8818562/140‑1 (MQ=255)

                                                                    tCAGGGAGTACTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGGACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                                <  2:7639806/140‑1 (MQ=255)

                                                                       gggAGTAATCAGTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTGTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGagag                                                             <  1:7006921/140‑1 (MQ=255)

                                                                        ggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGt                                                            >  1:9467141/1‑140 (MQ=255)

                                                                         gAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGta                                                           <  2:3653558/140‑1 (MQ=255)

                                                                          aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                          <  2:5513063/140‑1 (MQ=255)

                                                                           gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                         <  1:7877110/140‑1 (MQ=255)

                                                                           gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                         <  1:174254/140‑1 (MQ=255)

                                                                           gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                         <  1:9063369/140‑1 (MQ=255)

                                                                            tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                                  <  1:5174724/130‑1 (MQ=255)

                                                                             aaTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtg                                                       >  2:304593/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                      >  1:3218853/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                      >  2:8757642/1‑140 (MQ=255)

                                                                              aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAGTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgg                                                      >  1:8080295/1‑139 (MQ=255)

                                                                                    gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                                >  2:7863385/1‑140 (MQ=255)

                                                                                    gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                                >  2:8034415/1‑140 (MQ=255)

                                                                                      gcttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGca                                              >  2:488221/3‑139 (MQ=255)

                                                                                      gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCGGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTGGGTGTTAACATTCTCGAAATGCTCTGAGAGTATATTTGTTAAGcc                                              >  2:7124731/1‑140 (MQ=255)

                                                                                        ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCctt                                            <  1:4095095/140‑1 (MQ=255)

                                                                                           tGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                         >  2:5834436/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAATCATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                    >  2:977908/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                                    >  1:6370285/1‑140 (MQ=255)

                                                                                                cagcaCGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGGGTTAAGCCTTCTTCGGaa                                    >  2:2189656/1‑140 (MQ=255)

                                                                                                 agcaCGTCTTAAGCAAGAATATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAAt                                   >  1:1278441/1‑140 (MQ=255)

                                                                                                     cGTCTTAAGCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCttt                               >  1:11600333/1‑140 (MQ=255)

                                                                                                             gCAAGAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTg                       >  1:5279162/1‑140 (MQ=255)

                                                                                                                aGAACATGCGGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGATAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa                    >  2:939594/1‑140 (MQ=255)

                                                                                                                 gAACATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaaa                   >  1:5470695/1‑140 (MQ=255)

                                                                                                                  aaCATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                                   <  2:1882722/75‑1 (MQ=255)

                                                                                                                  aaCATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg                  <  1:3478461/140‑1 (MQ=255)

                                                                                                                    cATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGAt                <  1:11410693/140‑1 (MQ=255)

                                                                                                                    cATGCTGC‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGAt                <  2:9166760/140‑1 (MQ=255)

                                                                                                                      tgctgc‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc              >  1:6856616/1‑140 (MQ=255)

                                                                                                                           c‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgaga                                                              >  1:8982678/1‑87 (MQ=255)

                                                                                                                           c‑GGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTGTTGTGTTTGAAAAGATGCCTTTc         >  2:3199175/1‑140 (MQ=255)

ATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGC‑GGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTGATTGT  >  NZ_CP025268/581069‑581337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`