BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	833,226	T→C	378 (TAA→TAG)	ybhS ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	833,226	0	T	C	99.7%	2362.7 / NA	681	378 (TAA→TAG)	ybhS	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (311/368); minor base A (1/1); total (312/369)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 681 total aligned reads displayed.

GCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NZ_CP025268/833089‑833361

     gcggcgAACGGGACCAGGATCCCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGGTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGAGTCACGTCCATAAGCGATGAAACCTGCTCTTCTCCCTCATCCa                                                                                                                                  >  2:10393351/1‑140 (MQ=255)

     gcggcgAACGGGAACAGGATCACCTGAATTAGCACGTGAAAAATCAGAATCGCTCGGTTTTGCGGATCGCGCAGCAACGACTGCAAATATATGCGCATTAACGACCATAAGCGAAGAAACATGCTCTTCTCCCTAAACCa                                                                                                                                  >  2:7319045/1‑140 (MQ=255)

     gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:8788093/1‑140 (MQ=255)

     gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                  >  1:1762068/1‑140 (MQ=255)

       ggcgAACGGGAACAGGATCACCTGAATTCGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGc                                                                                                                                <  1:6797035/140‑1 (MQ=255)

                    aGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCATCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTCATCCAGCCGACGTTTGGttt                                                                                                                   <  1:2011916/140‑1 (MQ=255)

                    aGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGttt                                                                                                                   <  1:7725562/140‑1 (MQ=255)

                    aGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGttt                                                                                                                   <  1:8180000/140‑1 (MQ=255)

                      gATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCCCCCTAATCCAGCCGACGTTTGGTTTTc                                                                                                                 >  2:4283609/1‑140 (MQ=255)

                      gATCACCTGAATAAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTc                                                                                                                 >  1:477972/1‑140 (MQ=255)

                       aTCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGATCGCGCAGCAACGACTGCAACTCTTAGCTGATAAACGACCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCa                                                                                                                >  2:6217468/1‑140 (MQ=255)

                        tCACCTGAATTAGCTCTGGTAAAATCAGCATCGCGCGGGTGTTCGGTTCGCGCAGCAAAGACTTCTACTCTTTTCGGATTAACTTCCATCAGCTATGAAAAATGCTCTTCTACCTAAAACAGCCGACGTTTGGTTTTCAg                                                                                                               <  2:1504001/140‑1 (MQ=255)

                        tCACCTGAATTAGCACGGGTAAACTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                               <  1:4961924/140‑1 (MQ=255)

                        tCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAg                                                                                                               <  1:6232178/140‑1 (MQ=255)

                          aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                             >  2:7441606/1‑140 (MQ=255)

                          aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                             >  1:9275431/1‑140 (MQ=255)

                          aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACAGCAACTCTTTGAGGATTAACGACCATAAGCGATGAAACATGATCTTCTCACTAAACCAGACGACGTTTGGTTTTCAGcc                                                                                                             >  2:7569015/1‑140 (MQ=255)

                           ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                            >  1:5911115/1‑140 (MQ=255)

                           ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                            >  1:9825865/1‑140 (MQ=255)

                              gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                         >  1:1361218/1‑140 (MQ=255)

                               aaTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTc                                                                                                        <  2:1692529/140‑1 (MQ=255)

                                   aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGc                                                                                                    <  1:5640025/140‑1 (MQ=255)

                                    gCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGcc                                                                                                   >  2:3667606/1‑140 (MQ=255)

                                     cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  >  2:842213/1‑140 (MQ=255)

                                     cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                  >  2:10704503/1‑140 (MQ=255)

                                      aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                 >  2:1336608/1‑140 (MQ=255)

                                      aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                 >  1:1465316/1‑140 (MQ=255)

                                      aCGGGTAAAATCAGAATCGCGCGGGTTTGAGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                 >  2:5341797/1‑140 (MQ=255)

                                          gTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaaa                                                                                             >  1:603540/1‑140 (MQ=255)

                                          gTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaaa                                                                                             >  2:5692061/1‑140 (MQ=255)

                                             cactCAGAAAAGAGCGGGTTTGCGGGTCTCGCAGCAACGACTGCAAGTCTTAGCGGCTGAACGTCCATCATCGATGAAACATGCTCTTCTCCCTAATCCATCCGACGTTTGGTTTTAAGCCACGTCAGGCCGATAAACAt                                                                                          <  2:5554798/137‑1 (MQ=255)

                                              aaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATc                                                                                         >  2:40772/1‑140 (MQ=255)

                                                        gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  2:2682856/140‑1 (MQ=255)

                                                        gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  1:2714572/140‑1 (MQ=255)

                                                        gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  1:11014432/140‑1 (MQ=255)

                                                        gcgagGGTTTGCGGTTCGCGCAGCAAAGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                               <  2:9854374/140‑1 (MQ=255)

                                                                   cGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAAc                                                                    <  1:257638/140‑1 (MQ=255)

                                                                   cGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAAc                                                                    <  1:1613097/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  2:1101970/140‑1 (MQ=255)

                                                                       tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                                <  1:6481468/140‑1 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAGCATCTCCGCCGATGCGATCAAAAACAGCAc                                                               >  1:8500801/1‑140 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGctc                                                               >  2:4472693/1‑138 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  1:10826409/1‑140 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  1:8853262/1‑140 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                               >  1:4577708/1‑140 (MQ=255)

                                                                        cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGAGCAAAAACAGCAc                                                               >  2:5091651/1‑140 (MQ=255)

                                                                           gcagcaACGACTGCATCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGtt                                                            <  2:11194590/140‑1 (MQ=255)

                                                                            cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                           <  2:2829419/140‑1 (MQ=255)

                                                                            cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                           <  1:4173078/140‑1 (MQ=255)

                                                                                 aCGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTaccac                                                      <  2:9337949/140‑1 (MQ=255)

                                                                                    aCTGCATCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                   <  2:11434752/140‑1 (MQ=255)

                                                                                      tGCAACTCTTTGCGGATTAACGTCCTTCCGCGATGAAACCTGCTCTACTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGca                                                 <  2:1823236/140‑1 (MQ=255)

                                                                                       gCAACTCTTTGCGGATTAACGTCCTTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  2:10828738/140‑1 (MQ=255)

                                                                                       gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  1:4384204/140‑1 (MQ=255)

                                                                                       gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                >  2:4624089/1‑140 (MQ=255)

                                                                                       gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  1:5525102/140‑1 (MQ=255)

                                                                                       gCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  2:8048406/140‑1 (MQ=255)

                                                                                       gCAACACTTTGCGGATTAACGTCCATAAGCGATGACACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGATTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcac                                                <  2:8586058/140‑1 (MQ=255)

                                                                                         aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                              >  1:10736583/1‑140 (MQ=255)

                                                                                         aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCGTCTCCCTAATCCAGCCGACGGTTGGTTTTCAGCCACGTCAGGCCGATACACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                              >  1:3536451/1‑140 (MQ=255)

                                                                                           ctctTTGCGGAGTAACGTCCATACGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTATGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGATGCGATCAAAAACAGCACGATTACCACCAGcaccacg                                            >  2:3927077/1‑139 (MQ=255)

                                                                                            tctTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTg                                           >  1:6674137/1‑140 (MQ=255)

                                                                                             ctTTGCGGATTAANGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTgg                                          >  1:10007804/1‑140 (MQ=255)

                                                                                                tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                       >  1:7307052/1‑140 (MQ=255)

                                                                                                tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                       >  1:7986440/1‑140 (MQ=255)

                                                                                                     aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTcc                                  >  2:6839473/1‑140 (MQ=255)

                                                                                                     aTTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTcc                                  <  1:2458417/140‑1 (MQ=255)

                                                                                                        aaCGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCgg                               >  1:11491506/1‑140 (MQ=255)

                                                                                                         aCGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                              <  1:7001116/140‑1 (MQ=255)

                                                                                                          cGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCg                             <  2:124948/140‑1 (MQ=255)

                                                                                                          cGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCg                             <  2:8837661/140‑1 (MQ=255)

                                                                                                          cGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCg                             <  1:296427/140‑1 (MQ=255)

                                                                                                              cATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa                         <  1:751473/140‑1 (MQ=255)

                                                                                                               cgaaGCGATGAAACATGCTCTACTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAACACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                        <  2:10162986/138‑1 (MQ=255)

                                                                                                               aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGac                        >  2:10565361/1‑139 (MQ=255)

                                                                                                               aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                        >  1:389589/1‑140 (MQ=255)

                                                                                                               aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                        >  2:6465873/1‑140 (MQ=255)

                                                                                                                tAAGCTATGAATCATGCTCTTCTCCCTAATCCAGCCGACGATTGGTTTTCAGCCACGTCAGGCCTATAAACATCACCGCCGAAGCGATCAAAAACAGCCCGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                       <  2:343396/140‑1 (MQ=255)

                                                                                                                 aaGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACa                      <  1:105231/140‑1 (MQ=255)

                                                                                                                  aGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                     <  1:3200112/140‑1 (MQ=255)

                                                                                                                  aGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                     <  1:11272434/140‑1 (MQ=255)

                                                                                                                  aGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                     <  2:4802908/140‑1 (MQ=255)

                                                                                                                  aGCGATGAAACATGCTCTTCGCCCTAATCCCGCCGACGTTTGGTGTTCAGCCACGTCAGGCCGAGAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAg                     <  2:10283934/140‑1 (MQ=255)

                                                                                                                   gCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAgg                    <  1:3766838/140‑1 (MQ=255)

                                                                                                                   gCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAgg                    <  2:8206721/140‑1 (MQ=255)

                                                                                                                      aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGTGGAACAGGCtt                 >  1:10139946/1‑140 (MQ=255)

                                                                                                                      aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGACCAGGCtt                 >  1:10636730/1‑140 (MQ=255)

                                                                                                                        gAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACCGGCTTTg               >  1:8981625/1‑140 (MQ=255)

                                                                                                                         aaaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGc              <  1:1246416/140‑1 (MQ=255)

                                                                                                                         aaaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGc              <  1:5139364/140‑1 (MQ=255)

                                                                                                                          caCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGATTTCAGCCACGACAGGCCGATAAACAACACCGCCGAAGCGATCAAAAACAGCACGTTGACCACCACCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa             <  2:5193507/139‑1 (MQ=255)

                                                                                                                          aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAACCATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa             <  2:5977857/140‑1 (MQ=255)

                                                                                                                          aaCAGGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTGACCACCAGCACCACTGGAAGATTCCCGGCGAGGAACAGGCTTTGCa             <  2:2195700/140‑1 (MQ=255)

                                                                                                                               gCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGTCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTg        >  2:9065248/1‑140 (MQ=255)

                                                                                                                               gCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTg        >  1:951224/1‑140 (MQ=255)

                                                                                                                                 tcttctCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCt      >  2:7106950/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg  <  2:7777441/140‑1 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg  >  2:2546234/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACg  >  2:3106428/1‑140 (MQ=255)

                                                                                                                                     ctcCCTAATCCAGCCGACGGTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGGGAGGACCAGGCTTTGCAGGGTGCTGACg  >  1:8342078/1‑140 (MQ=255)

GCGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGACG  >  NZ_CP025268/833089‑833361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`