Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,235,822 G→A A216T (GCT→ACT)  ycgM → fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,235,8220GA99.0% 2313.4 / NA 618A216T (GCT→ACT) ycgMfumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (283/327);  minor base C (3/3);  total (287/331)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 618 total aligned reads displayed.

TTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAA  >  NZ_CP025268/1235685‑1235958
                                                                                                                                         |                                                                                                                                        
ttCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTNACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACt                                                                                                                                        >  1:6302735/1‑140 (MQ=255)
ttCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACt                                                                                                                                        >  2:1568158/1‑140 (MQ=255)
ttCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACt                                                                                                                                        >  1:5454764/1‑140 (MQ=255)
ttCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACt                                                                                                                                        >  2:8774935/1‑140 (MQ=255)
  ccGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTcg                                                                                                                                      <  1:4792587/140‑1 (MQ=255)
    gCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTcgcg                                                                                                                                    <  1:1003528/140‑1 (MQ=255)
    gCTGATCGCTAATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTcgcg                                                                                                                                    <  2:8478813/140‑1 (MQ=255)
      tGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGTGCATTCTTTGACAACTCGCGtt                                                                                                                                  <  2:3467756/140‑1 (MQ=255)
        aTCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGtttt                                                                                                                                <  1:8060080/140‑1 (MQ=255)
        aTCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGtttt                                                                                                                                >  1:6735072/1‑140 (MQ=255)
          cGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGt                                                                                                                              >  2:2566067/1‑140 (MQ=255)
           gCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTa                                                                                                                             <  2:9295997/140‑1 (MQ=255)
               atatGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATAc                                                                                                                         >  2:11170317/1‑140 (MQ=255)
                  tGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACttt                                                                                                                      >  2:4325084/1‑140 (MQ=255)
                    aGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACttttt                                                                                                                    >  2:6862010/1‑140 (MQ=255)
                     gCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGTCAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACTACTCGCGTTTTGTAATACTTTTTg                                                                                                                   <  1:4977668/140‑1 (MQ=255)
                      cacgTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACTGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgc                                                                                                                  <  2:7207240/137‑1 (MQ=255)
                      cAAGTTTTTTACCCTCATGGCCGGTGACGTTGTGTTGTCAGGCACGGCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCTGTGTGTGACAAGTCGCGTTGTGTAATAGTTTTTgc                                                                                                                  >  2:10027401/1‑140 (MQ=255)
                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgc                                                                                                                  <  2:8339328/140‑1 (MQ=255)
                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgc                                                                                                                  >  1:6926539/1‑140 (MQ=255)
                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgc                                                                                                                  <  2:10522888/140‑1 (MQ=255)
                      cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgc                                                                                                                  >  1:9744218/1‑140 (MQ=255)
                       aaGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTgcc                                                                                                                 >  1:742244/1‑140 (MQ=255)
                                    tCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcggc                                                                                                    >  1:4902787/1‑140 (MQ=255)
                                    tCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcggc                                                                                                    >  2:1744636/1‑140 (MQ=255)
                                    tCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCGGACAGTCACTTTCGATGGGCATTCGGTGACCCCTCGCGTTTTGTAATACTTGTTGCCGCCTGAAAgcggc                                                                                                    >  2:7107168/1‑140 (MQ=255)
                                    tCAAGGCCGGTGACGGTGTGGTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcggc                                                                                                    >  1:3205079/1‑140 (MQ=255)
                                           cGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTGTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCtaac                                                                                             >  2:9493507/1‑139 (MQ=255)
                                            ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAAc                                                                                            >  1:10215703/1‑140 (MQ=255)
                                             gTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACt                                                                                           >  2:2332847/1‑140 (MQ=255)
                                              tGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACtt                                                                                          >  1:3941338/1‑140 (MQ=255)
                                              tGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACtt                                                                                          >  1:2705383/1‑140 (MQ=255)
                                              tGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACtt                                                                                          >  2:601515/1‑140 (MQ=255)
                                                  gTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCAt                                                                                      <  1:10736695/140‑1 (MQ=255)
                                                  gTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCAt                                                                                      >  1:1722991/1‑140 (MQ=255)
                                                   ttGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATc                                                                                     >  1:6079918/1‑140 (MQ=255)
                                                    tgtgTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGTCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                    <  1:7510742/140‑1 (MQ=255)
                                                    tgtgTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                    <  2:8069386/140‑1 (MQ=255)
                                                    tgtgTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                    <  1:5862016/140‑1 (MQ=255)
                                                    tgtgTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                    <  1:3017796/140‑1 (MQ=255)
                                                     gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCAGCCTGAAAGCGGCGGCAAAACTTGCATCGc                                                                                   >  2:5231215/1‑140 (MQ=255)
                                                        ttGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgt                                                                                >  1:7791595/1‑140 (MQ=255)
                                                          gACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGc                                                                              <  2:473398/140‑1 (MQ=255)
                                                           aCAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGcc                                                                             >  2:5546059/1‑140 (MQ=255)
                                                             aGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAg                                                                           >  2:3268779/1‑140 (MQ=255)
                                                               gCACGCCTGCTGGCGTCGGCCCGGTGCAAAGCGGTGATGAGCTGCCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTGTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  2:6385109/140‑1 (MQ=255)
                                                               gCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCTCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  2:1402012/140‑1 (MQ=255)
                                                               gCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  2:4503179/140‑1 (MQ=255)
                                                               gCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  1:10889605/140‑1 (MQ=255)
                                                               gCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  1:5331186/140‑1 (MQ=255)
                                                               gCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  1:1150959/140‑1 (MQ=255)
                                                                cACGCCTGATGGCGTCGGCCCGTTTCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACt                                                                        >  1:4067080/1‑140 (MQ=255)
                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACt                                                                        >  2:5860298/1‑140 (MQ=255)
                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACt                                                                        >  1:7681359/1‑140 (MQ=255)
                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACt                                                                        >  1:1955314/1‑140 (MQ=255)
                                                                cACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACt                                                                        >  2:1163643/1‑140 (MQ=255)
                                                                  cGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTgg                                                                      >  2:4982181/1‑140 (MQ=255)
                                                                   gCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGt                                                                     <  1:1211866/140‑1 (MQ=255)
                                                                       gATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTtata                                                                 <  1:9054757/140‑1 (MQ=255)
                                                                             gTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCAATTTAGATGGGCATTATTTTACAACTCGCGTTTTGTAATACTTTTTGACGCGTGATAGCGGCGGCTCAAATTGAATCGCTGTGACAGAATGGTTATAAgggga                                                           >  2:7573015/1‑137 (MQ=255)
                                                                              tCGGCCCGTTGCAAAGCGGTGATGATCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCTGACTGGATATAAGGTGCg                                                          >  2:10327734/1‑140 (MQ=255)
                                                                               cGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGAc                                                                         <  1:3653096/124‑1 (MQ=255)
                                                                               cGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGt                                                         >  2:3833982/1‑140 (MQ=255)
                                                                                      ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACg                                                  >  2:1992952/1‑140 (MQ=255)
                                                                                      ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACg                                                  >  2:5710755/1‑140 (MQ=255)
                                                                                       tGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGt                                                 >  2:1891406/1‑140 (MQ=255)
                                                                                       tGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGt                                                 <  1:8775575/140‑1 (MQ=255)
                                                                                          aaaGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAAt                                              <  2:3623850/140‑1 (MQ=255)
                                                                                            aGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATgg                                            >  1:2130474/1‑140 (MQ=255)
                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCCTTCTTTGACAACTCGCGTTGTGGAATACTTTTTGCCGCCTGAAAGCGGCGGCTAGTCTTGCATCGCTGTGGCAGCGTGGTGATAAGGTGCGTTTTAGCGTAATGGCGGaa                                       >  2:6132161/1‑140 (MQ=255)
                                                                                                 tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGaa                                       >  2:2794234/1‑140 (MQ=255)
                                                                                                  gatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAac                                      <  1:11311105/140‑1 (MQ=255)
                                                                                                    tgaGCTGACAGGCACTTTCGCTGGGCATTCTTTGACAACTCGCGCTTTGTACTACTTTTTGCCGCCGGACAGCGGCGGCAAAACTAGCATCGCTGTGCCAGACTGTTTATAAGGTGCGTTTTACCGGAATGGCGGCacac                                    <  2:962534/140‑1 (MQ=255)
                                                                                                      aGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCt                                  <  1:2303642/140‑1 (MQ=255)
                                                                                                        cTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAAt                                              <  2:9131381/126‑1 (MQ=255)
                                                                                                        cTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtga                                >  1:8633308/1‑140 (MQ=255)
                                                                                                         tGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgat                               <  2:4277771/140‑1 (MQ=255)
                                                                                                         tGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACGTTTTGCCGCCAGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgat                               <  2:3105250/140‑1 (MQ=255)
                                                                                                         tGACAGTCACTTTCGATGGGCATTCGTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgat                               <  2:7909649/140‑1 (MQ=255)
                                                                                                          gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATATGGTGCGTTTTAACGTAATGGCGGAACACCtgatg                              <  1:11065238/140‑1 (MQ=255)
                                                                                                          gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatg                              <  1:9218763/140‑1 (MQ=255)
                                                                                                          gACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatg                              <  1:6487926/140‑1 (MQ=255)
                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatga                             >  1:7263495/1‑140 (MQ=255)
                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatga                             >  2:6636926/1‑140 (MQ=255)
                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatga                             >  1:8197554/1‑140 (MQ=255)
                                                                                                           aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCtgatga                             >  1:9572737/1‑140 (MQ=255)
                                                                                                            cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAg                            <  1:6639357/140‑1 (MQ=255)
                                                                                                            cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTGTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAg                            <  1:5147549/140‑1 (MQ=255)
                                                                                                                  cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTACTGGCGGAACACCTGATGAGCGATGt                      >  2:10692256/1‑140 (MQ=255)
                                                                                                                  cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAg                            <  2:11377134/134‑1 (MQ=255)
                                                                                                                  cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGt                      <  1:10476780/140‑1 (MQ=255)
                                                                                                                  cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGt                      >  1:2462685/1‑140 (MQ=255)
                                                                                                                  cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACGTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGt                      >  1:1287454/1‑140 (MQ=255)
                                                                                                                         tGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTc               >  1:5598370/1‑140 (MQ=255)
                                                                                                                         tGGGCATTCATTGACAACGCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATTGCGGAACAACTGATGAGAGATGTACCTTTc               >  2:8893030/1‑140 (MQ=255)
                                                                                                                             cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGTCTGGTGATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGTTGTTCCTTTCTGGc           >  2:10938323/1‑140 (MQ=255)
                                                                                                                              aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa          <  1:3411793/140‑1 (MQ=255)
                                                                                                                              aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa          <  1:1568158/140‑1 (MQ=255)
                                                                                                                              aTTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa          <  1:5728317/140‑1 (MQ=255)
                                                                                                                                     gACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTaaa   >  1:4441114/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTCCGCTGATCGCTTATATGAGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAA  >  NZ_CP025268/1235685‑1235958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑