BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,496,208	G→A	R163K (AGG→AAG)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,496,208	0	G	A	98.8%	1502.0 / NA	410	R163K (AGG→AAG)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (111/294); minor base T (0/5); total (111/299)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 410 total aligned reads displayed.

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496072‑1496344

aCGATGCCTTACTCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  2:1379564/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  2:157051/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  1:8593965/140‑1 (MQ=255)

aCGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGa                                                                                                                                       <  1:348737/140‑1 (MQ=255)

   aTGCCTTACGCTTCAGAGGCCAATTTCGACCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaa                                                                                                                                    <  2:7155652/140‑1 (MQ=255)

   aTGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaa                                                                                                                                    <  1:2448618/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCACTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:7359836/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  >  2:3860988/1‑140 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  2:328583/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:1171239/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  <  1:1230269/140‑1 (MQ=255)

     gCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGacaaca                                                                                                                                  >  2:9691058/1‑140 (MQ=255)

      ccTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacac                                                                                                                                 >  1:6265181/1‑140 (MQ=255)

       cTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAacaca                                                                                                                                <  2:4278955/140‑1 (MQ=255)

          aCGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCTGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACtt                                                                                                                             >  2:11300925/1‑140 (MQ=255)

          aCGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACtt                                                                                                                             <  1:4034381/140‑1 (MQ=255)

            gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           >  1:10284003/1‑140 (MQ=255)

            gCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCt                                                                                                                           <  2:10455642/140‑1 (MQ=255)

                cAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGGCAACACACTTCTACGc                                                                                                                       >  1:11435139/1‑140 (MQ=255)

                   agGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatg                                                                                                                    >  1:10552612/1‑140 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGTAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  2:12486/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  1:8762585/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  2:3888401/140‑1 (MQ=255)

                      ccAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCatgatg                                                                                                                 <  1:4984141/140‑1 (MQ=255)

                        aaTTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGcc                                                                                                               >  2:6572676/1‑140 (MQ=255)

                          tttCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCcgc                                                                                                             <  1:7619727/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        >  1:9089873/1‑140 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:9707742/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        >  1:8530555/1‑140 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:5803249/140‑1 (MQ=255)

                               aaCGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTaaa                                                                                                        <  1:4260369/140‑1 (MQ=255)

                                 cGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATAGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAAct                                                                                                      <  2:4117233/140‑2 (MQ=255)

                                    gTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTc                                                                                                   >  2:743532/1‑140 (MQ=255)

                                      aGATAGCATAAAGTCGTGGCAAGAAACGCTGGCGAATCTGGGATAGTTCGTCCTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTACCt                                                                                                 <  2:10891159/140‑1 (MQ=255)

                                          aGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAg                                                                                             <  2:4593298/140‑1 (MQ=255)

                                            cTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGa                                                                                           >  2:3166407/1‑140 (MQ=255)

                                             cttaaGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGat                                                                                          >  1:3872545/4‑140 (MQ=255)

                                              tAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGata                                                                                         >  2:9426690/1‑140 (MQ=255)

                                                aaGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATAtt                                                                                       <  1:8408818/140‑1 (MQ=255)

                                                  gTCGTGGCATGAAACGCGGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTa                                                                                     <  2:11623225/140‑1 (MQ=255)

                                                      tGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGa                                                                                 >  2:10398450/1‑140 (MQ=255)

                                                            gAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGATGCACTGGCACACCACTATTTCTGGAATGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGc                                                                           <  2:6843033/140‑1 (MQ=255)

                                                             caaCGCTGGCGAAACTGGGACAATTTGTCACCGGGTAGCAGGCTGCGGCTGCACTGGCACACCACGATTTCTGGAAGGACAAAACACTTCTACGCATGATGCCCCGTAAACGTTCCTGAAGGATATTTAAAGAAACCGcc                                                                          <  2:11057764/139‑1 (MQ=255)

                                                             aatcGCTGGCGATTCTTGGTTATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTTTTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTATACGTTCCTGAAGGATATTTTTAGAAAACGcc                                                                          <  2:10586858/137‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGATGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:2448815/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:10143412/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:9772028/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:11270554/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  2:6423742/140‑1 (MQ=255)

                                                             aaaCGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          <  1:3049317/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGTATATTTTGTAATCGGGTTGCACGCTGCGGATGCACTGGCACACAACTATTTCTGTAAGGACAACACAATTCTACGCATGATGCCGCTTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc                                                                     <  2:4167913/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTAc                                                                     <  2:7909041/140‑1 (MQ=255)

                                                                  cTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCAAACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAATAAAACGCCTGTAc                                                                     <  2:5514257/140‑1 (MQ=255)

                                                                   tGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACt                                                                    >  1:4534106/1‑140 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  1:6127861/140‑1 (MQ=255)

                                                                          tCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCg                                                             <  2:11352406/140‑1 (MQ=255)

                                                                            tGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAc                                                           <  2:1725330/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  1:8900132/140‑1 (MQ=255)

                                                                             gggATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGAcc                                                          <  1:11120777/140‑1 (MQ=255)

                                                                               gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTTTTGCTGGAAGGACAACACACGTCTACGCATGATGCCGCGTAAACGTTCCTGATGGATATTTAAAGAAACCGCCTGTACTAAAACCGACCCg                                                        <  2:10774432/140‑1 (MQ=255)

                                                                               gATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCg                                                        <  1:6204575/140‑1 (MQ=255)

                                                                                   ttttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGt                                                    >  1:7011007/1‑140 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGATGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:5377838/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:10736179/140‑1 (MQ=255)

                                                                                    tttGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACAACTCTATTTATGGAATGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTTTACTAAAACCGACCCGTGGTa                                                   <  2:8517140/140‑1 (MQ=255)

                                                                                    tttGTCAGCGGGTTGCACGCGGCGGCTGCACTGGCACACCACTATGTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAACCGCCTGTACTAAAACCGACCCGTGGTa                                                   <  2:3723295/140‑1 (MQ=255)

                                                                                    ttggTCATCGGGTTGCACGCGGCGGCTGAACTGGCACACCACTATTTCTGCAAGGACAACACACTTCTACGCATGATGCCGCTTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTa                                                   <  1:2099928/137‑1 (MQ=255)

                                                                                      tGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAGACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACa                                                 <  1:6558786/140‑1 (MQ=255)

                                                                                       gTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAg                                                <  2:8826706/140‑1 (MQ=255)

                                                                                       gTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCAATATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAg                                                <  1:2694798/140‑1 (MQ=255)

                                                                                        tCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAgg                                               >  1:1552111/1‑140 (MQ=255)

                                                                                        acATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAgg                                               <  1:9322169/139‑1 (MQ=255)

                                                                                          atggggTTGCACGCTGCGGCTGCACTGGCACACAAATATTTCTTTAAGGAAAAAACACTTATACGCTTTATGCCGCTTAAAGTTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                             <  1:3166408/137‑1 (MQ=255)

                                                                                          aTCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCg                                             <  1:1882435/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGTAAGGAAAACACAATTATACGCTTGTTGCCGCTTAAACGTTCCTGAAGTATTTTTAAATAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:2370478/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCTTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  1:536264/140‑1 (MQ=255)

                                                                                                 tGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATa                                      <  2:2636270/140‑1 (MQ=255)

                                                                                                     cGCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACggg                                  >  2:9147686/1‑140 (MQ=255)

                                                                                                      gCTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACAAACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGt                                 <  2:9829883/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCTTGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:300101/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATTATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:602133/140‑1 (MQ=255)

                                                                                                       cTGCGGCTGCACTGCCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTc                                <  1:7148804/140‑1 (MQ=255)

                                                                                                        tGCGGCTGCACTGGCACACCACTCTTTCTGGAAGGACAACACACTTCTACGCAGGATGCCGCGTAATCGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCt                               >  2:5077273/1‑140 (MQ=255)

                                                                                                            gCTGCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGTATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACAt                           <  1:9269279/140‑1 (MQ=255)

                                                                                                               gCACTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCgg                        <  2:8237333/140‑1 (MQ=255)

                                                                                                                  cTGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGTTCTACATCGGAAg                     <  1:10013296/140‑1 (MQ=255)

                                                                                                                   tGGCACACCACTATTTCTGGAATGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgc                    <  1:7917577/140‑1 (MQ=255)

                                                                                                                   tGGCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCTTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgc                    <  1:5609535/140‑1 (MQ=255)

                                                                                                                    ggCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcg                   >  1:2839877/1‑140 (MQ=255)

                                                                                                                     gCACACCACTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  <  2:3500499/140‑1 (MQ=255)

                                                                                                                        caccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGACGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta               <  1:7250005/140‑1 (MQ=255)

                                                                                                                        caccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta               <  2:61061/140‑1 (MQ=255)

                                                                                                                        caccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCta               <  1:4762441/140‑1 (MQ=255)

                                                                                                                        caccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGCCGAAGAATACGGGTCTACATCGGAAGCGCCta               <  1:9628931/140‑1 (MQ=255)

                                                                                                                          ccacTATTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCtatt             >  2:2140492/1‑140 (MQ=255)

                                                                                                                               aTTTCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAgcgc                  >  1:6689462/1‑130 (MQ=255)

                                                                                                                                tttCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtattt       >  1:4161138/1‑140 (MQ=255)

                                                                                                                                  tCTGGAAGGACAACACACTTCTACGCATGATGCCGCGTATACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTAtatttat     <  2:7764391/140‑1 (MQ=255)

                                                                                                                                     ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGcc                                                                          >  2:8168676/1‑68 (MQ=255)

                                                                                                                                     ggAAGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGTAAGCGCCTATTATATTTATTTg  >  2:5005138/1‑140 (MQ=255)

ACGATGCCTTACGCTTCAGAGGCCAATTTCGAACGGGTAGATAGCTTAAAGTCGTGGCATGAAACGCTGGCGAATCTGGGATATTTTGTCATCGGGTTGCACGCTGCGGCTGCACTGGCACACCACTATTTCTGGAGGGACAACACACTTCTACGCATGATGCCGCGTAAACGTTCCTGAAGGATATTTAAAGAAAACGCCTGTACTAAAACCGACCCGTGGTACAGGCGAAGAATACGGGTCTACATCGGAAGCGCCTATTATATTTATTTG  >  NZ_CP025268/1496072‑1496344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`