BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,620,861	T→C	A93A (GCT→GCC)	yneK →	putative protein YneK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,620,861	0	T	C	99.7%	1260.1 / NA	363	A93A (GCT→GCC)	yneK	putative protein YneK
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (188/174); minor base A (0/1); total (188/175)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

ACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGC  >  NZ_CP025268/1620724‑1620997

aCGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAg                                                                                                                                        <  2:4487161/140‑1 (MQ=255)

  gTCTTTAACCATCAACTCAGCCACAAAAAAGAGATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGa                                                                                                                                      <  1:1472440/140‑1 (MQ=255)

  gTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGa                                                                                                                                      >  2:3430340/1‑140 (MQ=255)

  gTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGa                                                                                                                                      >  1:10667928/1‑140 (MQ=255)

  gTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGa                                                                                                                                      >  1:5715097/1‑140 (MQ=255)

      atAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATgg                                                                                                                                  <  1:1108300/139‑1 (MQ=255)

       tAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGa                                                                                                                                 >  1:3953977/1‑140 (MQ=255)

        aaaCATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGaa                                                                                                                                <  1:7269994/140‑1 (MQ=255)

             tCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGacac                                                                                                                           <  2:1855139/140‑1 (MQ=255)

              cAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGAcaca                                                                                                                          >  2:7103239/1‑140 (MQ=255)

              cAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGAcaca                                                                                                                          >  1:10123410/1‑140 (MQ=255)

                     gCCACAAAAAAGATATTTAATAACCTTGACAACTTCTCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGaaaa                                                                                                                   >  1:7587357/1‑140 (MQ=255)

                        acaAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAatat                                                                                                                <  2:301104/140‑1 (MQ=255)

                         caAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGa                                                                                                                              <  2:2172969/125‑1 (MQ=255)

                         caAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATAtt                                                                                                               <  1:2394462/140‑1 (MQ=255)

                          aaaaaaGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTg                                                                                                              >  2:6991593/1‑140 (MQ=255)

                               aGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAAttt                                                                                                         <  2:4776089/140‑1 (MQ=255)

                               aGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAAttt                                                                                                         <  1:10623068/140‑1 (MQ=255)

                               aGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAAttt                                                                                                         <  1:4195417/140‑1 (MQ=255)

                                   atataataaCCTTGACAACTTATCATATATTCAGAGGACTTCTTAGCGGCGTAACGACACGCTATACAATTAAAAGGTTCAGTTTAAACTCACTTATCCCGCCATGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:6726242/137‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:5068615/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:5460388/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:11140252/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  1:8266743/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  1:5148501/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  1:6690697/140‑1 (MQ=255)

                                   atTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:9287566/140‑1 (MQ=255)

                                    tttAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                                    <  1:1929091/140‑1 (MQ=255)

                                           aCCTTGTCAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaata                                                                                             <  1:4924665/140‑1 (MQ=255)

                                           aCCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaata                                                                                             >  1:247936/1‑140 (MQ=255)

                                           aCCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaata                                                                                             <  2:5759690/140‑1 (MQ=255)

                                           aCCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaata                                                                                             <  1:3655518/140‑1 (MQ=255)

                                            ccTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaataa                                                                                            >  1:8485776/1‑140 (MQ=255)

                                            ccTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaataa                                                                                            >  2:5312978/1‑140 (MQ=255)

                                                gACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAAttt                                                                                        <  1:6053472/140‑1 (MQ=255)

                                                gACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAAttt                                                                                        <  2:3990228/140‑1 (MQ=255)

                                                gACAACTTATCATATATTCAGAGGACTTATTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAAttt                                                                                        <  1:8754573/140‑1 (MQ=255)

                                                   aaCTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAg                                                                                     <  2:2506413/140‑1 (MQ=255)

                                                   aaCTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAg                                                                                     <  2:1533168/140‑1 (MQ=255)

                                                    aCTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTCTCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGc                                                                                    <  1:3305686/140‑1 (MQ=255)

                                                    aCTTATCATATATTCACATGACTTATTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGc                                                                                    <  2:8973433/140‑1 (MQ=255)

                                                       tATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGacac                                                                                                                           >  2:5511590/1‑98 (MQ=255)

                                                       tATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGacac                                                                                                                           <  1:5511472/98‑1 (MQ=255)

                                                       tATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCt                                                                                 >  1:5919671/1‑140 (MQ=255)

                                                       tATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCt                                                                                 >  1:3800523/1‑140 (MQ=255)

                                                       tATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCt                                                                                 >  1:4435674/1‑140 (MQ=255)

                                                        aTCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCta                                                                                <  2:10748364/140‑1 (MQ=255)

                                                         tCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCtat                                                                               <  1:9209283/140‑1 (MQ=255)

                                                           atatatTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCtattt                                                                             <  1:10792445/140‑1 (MQ=255)

                                                               atTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTctttattt                                                                         >  2:10281804/1‑140 (MQ=255)

                                                               atTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTatttattt                                                                         >  1:9307713/1‑140 (MQ=255)

                                                                ttCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTAtttattta                                                                        >  1:11210171/1‑140 (MQ=255)

                                                                   agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                                     <  2:4172892/108‑1 (MQ=255)

                                                                   agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGAt                                                                     >  1:9723209/1‑140 (MQ=255)

                                                                   agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGAt                                                                     >  2:10697025/1‑140 (MQ=255)

                                                                     agGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGACTGGAAGACACAAAGAAAATATGGAAGTTCAGGTAGTAATAAATTGTAGCCCTAGTTATTTAGATaa                                                                   >  2:6765577/1‑140 (MQ=255)

                                                                     agGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATaa                                                                   >  1:2136641/1‑140 (MQ=255)

                                                                     agGACTTCTTTTCGGGGTAAAGACAAGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAACACACAAAGAAAATATTGAATTACAGGTCGTAATAAATGTCAGCCCTATTTAGTTCGATaa                                                                   >  2:7812586/1‑140 (MQ=255)

                                                                       gACTACTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAAtt                                                                 >  2:6483340/1‑140 (MQ=255)

                                                                        aCTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAAttt                                                                >  1:6285297/1‑140 (MQ=255)

                                                                         cttcttTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaata                                                                                             >  2:4545034/1‑110 (MQ=255)

                                                                          ttcttTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATCTTGAATTTCAGGTAGTAATCAATTTAAGCCCTATTTCTTTAGATAATTTTc                                                              >  2:7166995/1‑140 (MQ=255)

                                                                          ttcttTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTc                                                              >  2:2737622/1‑140 (MQ=255)

                                                                              tttCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCa                                                          <  1:7019301/140‑1 (MQ=255)

                                                                                    ggTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGAggg                                                    >  1:1568606/1‑140 (MQ=255)

                                                                                       aaCGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgta                                                                                                   <  2:9870894/90‑1 (MQ=255)

                                                                                                cTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAatat                                        <  1:7134670/140‑1 (MQ=255)

                                                                                                   taCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGACTTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATAtttt                                     >  2:8074035/1‑140 (MQ=255)

                                                                                                   taCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATAtttt                                     >  1:5426518/1‑140 (MQ=255)

                                                                                                   taCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATAtttt                                     >  2:8487136/1‑140 (MQ=255)

                                                                                                    aCAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATAttttt                                    >  2:173675/1‑140 (MQ=255)

                                                                                                       aTGAACAGGTTCAGTTTAAACGCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgc                                 <  2:9625332/140‑1 (MQ=255)

                                                                                                       aTGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgc                                 >  1:9527270/1‑140 (MQ=255)

                                                                                                       aTGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgc                                 <  1:1664991/140‑1 (MQ=255)

                                                                                                       aTGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgc                                 <  1:7900979/140‑1 (MQ=255)

                                                                                                        tgcaaaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATGTCTTTAGATAATTTTCGCCATGATGGCGAAATTAATATTTTTTgcg                                >  2:2068404/4‑140 (MQ=255)

                                                                                                        tGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGtaa                                                                                               >  1:3062579/1‑77 (MQ=255)

                                                                                                        tGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgcg                                >  2:2406940/1‑140 (MQ=255)

                                                                                                        tGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgcg                                >  1:11552261/1‑140 (MQ=255)

                                                                                                        tGAAAAGGTTCAGTTTAAACTCACTTATCACGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgag                                >  1:299760/1‑138 (MQ=255)

                                                                                                         gAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgcgc                               >  2:10551260/1‑140 (MQ=255)

                                                                                                         gAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTgcgc                               >  1:8571198/1‑140 (MQ=255)

                                                                                                           aaaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTc                             >  2:319273/1‑140 (MQ=255)

                                                                                                           aaaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTc                             >  2:3298085/1‑140 (MQ=255)

                                                                                                           aaaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTc                             >  1:6544796/1‑140 (MQ=255)

                                                                                                           aaaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTAGTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTc                             >  1:4349509/1‑140 (MQ=255)

                                                                                                            aaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcc                            <  1:10237404/140‑1 (MQ=255)

                                                                                                            aaGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcc                            <  2:8167013/140‑1 (MQ=255)

                                                                                                             aGGTTCTGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTATGCCGTATTTATTGAGATAATTTTCGCCTGGATGGAGTCATTATTTTTTTTTGAGCTccc                           >  2:11618548/1‑140 (MQ=255)

                                                                                                             aGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTccc                           >  1:8093748/1‑140 (MQ=255)

                                                                                                             aGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTccc                           >  1:2309426/1‑140 (MQ=255)

                                                                                                             aGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCAccc                           >  2:9382802/1‑140 (MQ=255)

                                                                                                                      ttAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAg                  <  1:9805309/140‑1 (MQ=255)

                                                                                                                       tAAACTCACTTATCCCGCCAGGAATGGAAGACACAAACAAAATATTTAATTTCAGGTAGTACTAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGc                 <  2:10123409/140‑1 (MQ=255)

                                                                                                                        aaaCTCACTTATCCCTCCAGGAAAGGAAGACACAAAGAAAATATTGCATTTAAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATTATTTGCGCCATGATGGAGAAATTAATATTTTTTGCTCTCCCAACCCAAAGcc                <  2:10897557/140‑1 (MQ=255)

                                                                                                                          aCTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTg              <  1:4625599/140‑1 (MQ=255)

                                                                                                                          aCTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTg              <  2:5825248/140‑1 (MQ=255)

                                                                                                                          aCTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTg              <  2:10019971/140‑1 (MQ=255)

                                                                                                                            tCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTc            <  1:3431690/140‑1 (MQ=255)

                                                                                                                                    cccGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATggg    <  1:8961312/140‑1 (MQ=255)

ACGTCTTTAAACATCAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCCTGTCACTATGGGGC  >  NZ_CP025268/1620724‑1620997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`