BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	99.4%	2273.7 / NA	657	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (318/335); minor base A (2/2); total (320/337)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 657 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGC‑G‑CCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          <  2:5000272/140‑1 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  1:9290801/1‑140 (MQ=255)

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTCTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                          >  1:4571546/1‑140 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg                                                                                                                                      <  1:4079728/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  2:2308894/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     <  1:5766148/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  1:1211674/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     <  1:5463309/140‑1 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc‑g‑c                                                                                                                                 >  1:2751112/1‑140 (MQ=255)

          aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑G‑ccc‑c                                                                                                                             <  2:6634581/140‑1 (MQ=255)

            aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑G‑ccc‑cca                                                                                                                           <  1:6432717/140‑3 (MQ=255)

            aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑G‑ccc‑cca                                                                                                                           <  1:10450288/140‑3 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑G‑ccc‑ccagcc                                                                                                                        >  1:144270/1‑135 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCg                                                                                                                       <  1:9620545/140‑1 (MQ=255)

                   gAGTGATCACAAAAAAAGGCGTATA‑TTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATCCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGcaa                                                                                                                    <  2:5108088/140‑1 (MQ=255)

                    aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGcaa                                                                                                                    <  1:9024742/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGcaaca                                                                                                                  <  2:10747258/140‑1 (MQ=255)

                                      cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGc                                                                                                  <  2:8396057/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGACTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 <  1:9477711/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCa                                                                                                 >  2:1469301/1‑140 (MQ=255)

                                        tataTTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCaa                                                                                                <  2:4456045/140‑1 (MQ=255)

                                         atatTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCaaa                                                                                               <  1:2045277/140‑1 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                        >  1:4341315/1‑140 (MQ=255)

                                                  cTGTGAATGGTTGACAAAAGATGAATTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                      >  2:6187461/1‑140 (MQ=255)

                                                  cTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                      >  2:10634134/1‑140 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                     <  1:3987918/140‑1 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   <  1:688760/140‑1 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCGTATTGATCATATGGATTTATGGTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCACCg                                                                                   >  2:6853545/1‑140 (MQ=255)

                                                     agAATGGTTGACAACAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                   <  1:7131853/139‑1 (MQ=255)

                                                      gAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGc                                                                                  >  1:7374671/1‑140 (MQ=255)

                                                       aaTGGTTGACAAAAGATGACATAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCCTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                 <  1:7694030/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                <  1:1148895/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                <  1:10621881/140‑1 (MQ=255)

                                                             tGACACAAGATGACAGAGACTCCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTGAGGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCACTCAACGCACCCGTc                                                                           <  2:8810855/140‑1 (MQ=255)

                                                             tGACAAATTATGAAATAGAATTCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTTTGTTCCTACGTGCTC‑CCC‑CAGCCCGCTACAATGATCAACTTTCCTAAAAGCGCAATCAACGCACCCGTc                                                                           <  2:7524103/140‑1 (MQ=255)

                                                             tGACAAAAGATGACATAGAATACCTTTTGCCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:3986729/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  1:8612736/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTGGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                           <  2:9699783/140‑1 (MQ=255)

                                                               acacAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGACGCTAAGCGCAATCAACGCACCCGTCCa                                                                         >  2:8083695/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTcct                                                                         >  2:4300720/1‑139 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  2:8904067/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  1:10980673/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                         >  1:4485743/1‑140 (MQ=255)

                                                                 aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATCATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                       >  1:8157437/1‑140 (MQ=255)

                                                                     gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                   >  2:1942500/1‑140 (MQ=255)

                                                                      aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                  >  1:5264785/1‑140 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                             <  2:4705620/140‑1 (MQ=255)

                                                                               taTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTTCGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                         <  1:10129859/139‑1 (MQ=255)

                                                                               aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                         >  1:3028661/1‑140 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                        >  2:7021401/1‑140 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                        >  2:9866039/1‑140 (MQ=255)

                                                                                  aCCTTTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                      <  2:5297593/140‑1 (MQ=255)

                                                                                   ccTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCa                                                     >  1:2456683/1‑140 (MQ=255)

                                                                                    cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                    >  2:3552704/1‑140 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGCTCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                   <  2:3099268/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                   <  2:10858194/140‑1 (MQ=255)

                                                                                      tttGTCAGCTGACGCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  1:9998876/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAg                                                                                              >  1:6568640/1‑96 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  >  2:5953745/1‑140 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                  <  2:123821/140‑1 (MQ=255)

                                                                                        tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCa                                                <  1:3668501/140‑1 (MQ=255)

                                                                                        tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCa                                                >  1:1315929/1‑140 (MQ=255)

                                                                                             gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                           >  1:7825371/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                          >  1:3567225/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGACCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                          >  2:7826222/1‑140 (MQ=255)

                                                                                                 acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                 <  1:1752544/130‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGCTCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:7282758/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:4818364/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:5837782/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:10825384/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGAAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  1:622567/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGGTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:9215516/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTGAGTGATACAATGGATTGATGGTCCTACGTGC‑GCCCC‑CAGCCCGCAACAGTGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                 <  2:6455471/140‑1 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCACAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  2:3420017/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                >  1:6002034/1‑140 (MQ=255)

                                                                                                        ctctTATCTTATTGATAAAATGGATTTATGTCCCTACGTGC‑G‑CCCACAGCCCGCAACAATGATCAACATGACGCAAAGCGCAATCAACGAACCCGTCCAGTCATAAAGAGTCAGTTTCACTCCATCCAAAACGCGCAGcc                                >  2:1135761/1‑140 (MQ=255)

                                                                                                               cttatTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                         >  2:9522499/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                       <  2:3737337/140‑1 (MQ=255)

                                                                                                                  atTGATAAATTGTATTTATCTTCCGACGTGC‑GACCA‑CAGCACGCAACAATGATCAACAAGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCTCTCCATCCACAACGCTCAGCAACATCAAAGc                      >  2:10454952/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      <  2:9083954/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  2:2026455/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  1:6146643/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  1:8378901/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                      >  2:11074242/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACAACAACGc                      >  2:10279613/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                     >  1:1902673/1‑140 (MQ=255)

                                                                                                                         aaaTGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAg               <  1:11100545/140‑1 (MQ=255)

                                                                                                                          aaTGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGa              >  2:2812776/1‑140 (MQ=255)

                                                                                                                           aTGGATTTATGTTCCTACGAGC‑GCCCC‑CAGCCCGCAACACTGATCAACATGCCGCCAAGCGCAATCAACTCACCCGTCCAGTCATAAAGAGTCAGATTCACTCCACCCACAACTCGCAGCCACATCAACGCCGTGCAGAc             <  2:1650218/140‑1 (MQ=255)

                                                                                                                            tGGATTTATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACa            <  1:4894924/140‑1 (MQ=255)

                                                                                                                              gATTTATGTTCCTACGTGCTC‑CCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCAAATAAACGCCGTGCAGACATa          <  2:2454745/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa        <  1:9832199/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa        <  1:10377680/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:5575687/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  1:8020471/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:9374305/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:10104503/140‑1 (MQ=255)

                                                                                                                                  gctGTTCCAACGAGC‑GCCCC‑CAGCCCGCACCCATGCTCACCCGGCCGCACAGCGCCATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg      <  2:8575584/138‑1 (MQ=255)

                                                                                                                                   aTGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCTCAGCGCAATCCACGCACACGTCCAGTCAGATAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAAAGACGTGCAGACAGAAACGc     >  2:2525930/1‑140 (MQ=255)

                                                                                                                                   aTGTTCCTACGTGC‑GCCCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCTTCCACAACGCGCAGCCACATCAACGCCGTNCAGACATAAACGc     <  1:10009431/140‑1 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGC‑G‑CCC‑CAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCAC  >  NZ_CP025268/1660029‑1660301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`