BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,675,399	A→G	R295G (AGA→GGA)	ynfM →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,675,399	0	A	G	99.7%	2546.2 / NA	733	R295G (AGA→GGA)	ynfM	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (410/321); minor base T (1/1); total (411/322)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 733 total aligned reads displayed.

TGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATT  >  NZ_CP025268/1675262‑1675535

 gCTGATGGGGTCGTTCCACACGCAGATTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGCTTGGCTTATTATCGCTGGCTTCTTTGACCGGTACATGGAGCTCACCCAAAGCCGGaa                                                                                                                                       >  1:4051520/1‑140 (MQ=255)

    gATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATTCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCa                                                                                                                                    >  1:4123478/1‑140 (MQ=255)

     aTGGGGTCGTTCGTAACGCTGTTTAATTACATCGGCTATCTGTTGTTGCTCTCCCCCTGGCTTGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGGTTATTTGACCGGTACATGGAGCTCACCCAAAGCAGGAACAAt                                                                                                                                   >  2:10695373/1‑140 (MQ=255)

        gggTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGac                                                                                                                                <  1:8330599/140‑1 (MQ=255)

           tcgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccac                                                                                                                             <  1:206702/140‑1 (MQ=255)

           tcgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCACGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccac                                                                                                                             >  2:3586191/1‑140 (MQ=255)

            cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCGGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                            <  2:7603159/140‑1 (MQ=255)

            cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                            <  1:1309094/140‑1 (MQ=255)

                       cTGTTTAATTACCTCGGCTATCGGTTGATGCTCGCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                 <  1:11574691/140‑1 (MQ=255)

                       cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTTGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACAACCCGCTATGGGCg                                                                                                                 <  2:2800868/140‑1 (MQ=255)

                       cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                 <  2:5504186/140‑1 (MQ=255)

                       cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                 >  2:1534245/1‑140 (MQ=255)

                       cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                 <  1:11436721/140‑1 (MQ=255)

                       cTGTTTAATTACATCGGCTAACGGTTGATGCTCTCCCCCTGGCATGTCAGACAGGCCGTGGGTGGCTTATTATCGCTGGCGTATTTGACCGGGACACGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                                 <  2:4635573/140‑1 (MQ=255)

                        tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGt                                                                                                                >  1:1430867/1‑140 (MQ=255)

                        tGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCAGGACCACCCGCTATGGGCGt                                                                                                                >  2:6402275/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCTTGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTGATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  2:4889345/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCATTCAGGCCGTGGTTGGCTTATTNTCGCTGGCTTATTTGACCGGTACATGGAGATCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGAGGTCCAGAGAtg                                                                                                    >  1:6308452/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  1:1647469/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  1:5549441/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  2:3952473/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  2:1033879/1‑140 (MQ=255)

                                    tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTAGTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                                    >  1:925775/1‑140 (MQ=255)

                                         tATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATgttgtt                                                                                               >  2:11013149/1‑140 (MQ=255)

                                         tATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATgttgtt                                                                                               <  1:4642002/140‑1 (MQ=255)

                                         tATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATgttgtt                                                                                               >  1:7670891/1‑140 (MQ=255)

                                          aTCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGttt                                                                                              >  1:5995530/1‑140 (MQ=255)

                                               ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                         <  2:8574191/140‑1 (MQ=255)

                                               ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATGTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                         <  2:6295478/140‑1 (MQ=255)

                                               ttGATGCTCTCCCCCTGGCAGGTCAGTCAGGCCGTGGGTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACACAAAGCCGGAACCAGGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                         <  2:8404775/140‑1 (MQ=255)

                                                  aTGATCTCCCCCTGGCATGTCAGTAAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGAAAGGTAAATGGAGCTCACCCAAAGACGGAAACATGAACACACGCTAAGGGCGTGGTCCAGTGATGTAGTTTTCGACtgg                                                                                      >  2:7224011/1‑140 (MQ=255)

                                                     ctctCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGt                                                                                   >  1:1752031/1‑140 (MQ=255)

                                                        tcCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTAt                                                                                >  1:4922726/1‑140 (MQ=255)

                                                         cccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                               >  2:3381559/1‑140 (MQ=255)

                                                         cccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCAGGACCCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTagg                                                                               >  2:9187314/1‑138 (MQ=255)

                                                             cTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGt                                                                           <  1:4509727/140‑1 (MQ=255)

                                                              tGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGCCCGGGACATGGAGCTCACCCAACGCCGGAACCCTGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                          <  1:3030610/140‑1 (MQ=255)

                                                              tGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                          >  1:9822335/1‑140 (MQ=255)

                                                              tGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGtt                                                                          <  1:10661418/140‑1 (MQ=255)

                                                               ggCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGttt                                                                         >  1:6787932/1‑140 (MQ=255)

                                                               ggCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCGTATGTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCGATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGttt                                                                         >  2:668690/1‑140 (MQ=255)

                                                                gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTg                                                                        >  2:9364276/1‑140 (MQ=255)

                                                                gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTg                                                                        >  2:1348219/1‑140 (MQ=255)

                                                                  ctgacagtcagGCCGTGGTTGGATTATTATCGCTGTCTTAATTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACAACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTTTGCTGTTTGGt                                                                      <  2:3102092/136‑1 (MQ=255)

                                                                   tGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGtt                                                                     <  1:10017765/140‑1 (MQ=255)

                                                                    gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                                    >  2:5375589/1‑140 (MQ=255)

                                                                    gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTGAGTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGAGGTCCAGTGATGGTGTGTTCGACGGGGGGGAGGCTGTTTGGttt                                                                    >  2:7780430/1‑140 (MQ=255)

                                                                     tcagtcagGCCGTGGTTGTCTTATTATCGCTGGCGTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGTTTTa                                                                   >  2:5150598/1‑140 (MQ=255)

                                                                     tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTa                                                                   >  2:6925790/1‑140 (MQ=255)

                                                                        gtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtg                                                                >  1:3922494/1‑140 (MQ=255)

                                                                         tcagTCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  2:8234290/140‑1 (MQ=255)

                                                                         tcagGCCGTTGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  1:3737609/140‑1 (MQ=255)

                                                                         tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTGTGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  1:1019777/140‑1 (MQ=255)

                                                                         tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGTTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  2:8771432/140‑1 (MQ=255)

                                                                         tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  1:3337544/140‑1 (MQ=255)

                                                                         tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  1:9478771/140‑1 (MQ=255)

                                                                         tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               <  2:2366231/140‑1 (MQ=255)

                                                                          cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTcc                                                                                                           >  1:7554259/1‑95 (MQ=255)

                                                                          cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCGATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTCTGCTGGTTGGTTTACtgat                                                              >  1:6139959/1‑140 (MQ=255)

                                                                          cagGCCGTGGTTGGCGTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTAGGGGCGTGGTCCAGTGATGTTGGTGGCGACGGGGGTTATGCTGTTTGGTTTACtgat                                                              >  2:2231290/1‑140 (MQ=255)

                                                                           agGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGac                                                                                                                                <  2:10580793/73‑1 (MQ=255)

                                                                              ccGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGAcc                                                          <  1:1043884/140‑1 (MQ=255)

                                                                              ccGTGGTTGGCTTATTATCGCTGGCTTATTTGACCAGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGAcc                                                          <  2:4254930/140‑1 (MQ=255)

                                                                                 tGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTa                                                       >  1:8044185/1‑140 (MQ=255)

                                                                                   gTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTAtt                                                     >  2:6036562/1‑140 (MQ=255)

                                                                                       gCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAATGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGc                                                 >  1:5699137/1‑140 (MQ=255)

                                                                                            ttatCGCTGGCTTATTTGACCGGTACATGGAGCGCACCCAAAGCCGGACCCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGGGTCCTGAGGACCTTATACAGCTCGcg                                            <  2:4446863/140‑2 (MQ=255)

                                                                                             tatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                           >  1:3170477/1‑140 (MQ=255)

                                                                                             tatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                           >  2:974627/1‑140 (MQ=255)

                                                                                                 gCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAGGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGCCGGGGGTGATGCTGTTTGGTTGACTGATGACCTTAGTCAGCTCGCTGTGGc                                       >  2:8964079/1‑140 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                           >  1:2579571/1‑135 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCt                                      >  1:5822771/1‑140 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCt                                      >  1:9698797/1‑140 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGGTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCt                                      >  2:2536710/1‑140 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGGGATGTTGTTTTCGACGGGGGTTATGCTGTGTGGTTTACTGATGACCTTCTTCAGCTCGCTGTGGCt                                      >  2:7946092/1‑140 (MQ=255)

                                                                                                  cTGGCTTATTTGACCGGTACATGGAGCTAACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCtg                                           >  1:352687/1‑135 (MQ=255)

                                                                                                     gCTTATTGGACCGGTACATGGAGCTCTCCAAAATCCGGAACCAGGACCACCCGATATTGGCGTTGTCCATTGATGTTTTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGAACTTATTCAGCTCGATGTGGCTGAt                                   <  2:181301/140‑1 (MQ=255)

                                                                                                          tttGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTGATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTg                              >  2:3720935/1‑140 (MQ=255)

                                                                                                             gACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                           <  1:7852166/140‑1 (MQ=255)

                                                                                                             gACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                           <  2:1681579/140‑1 (MQ=255)

                                                                                                             gACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                           <  1:11223434/140‑1 (MQ=255)

                                                                                                                cGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGaa                        <  2:5693354/140‑1 (MQ=255)

                                                                                                                     cATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTa                   >  1:10930745/1‑140 (MQ=255)

                                                                                                                            cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTTTTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  1:8997757/1‑140 (MQ=255)

                                                                                                                            cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  2:4180467/1‑140 (MQ=255)

                                                                                                                            cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  1:6636441/1‑140 (MQ=255)

                                                                                                                            cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct            >  2:1233594/1‑140 (MQ=255)

                                                                                                                             tctcccAAAGCCGTAACCATGACAACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGGTTACTGATGACCTTATTCAGCTCGCTGTGGAGGATCTTTGCGTGATTGTTACTCTtctc           >  2:10087245/4‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGACCCATGACCACCCGCTATGGTCGTGGACCAGTGAGTTTGTTTTCGACGTGTCTTAGGCTGTTTGTTTTACTGATGACCTTATTCAGCTCTCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:11384270/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                               >  1:81668/1‑88 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGACTGTTACTCTtctc           >  1:7409887/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:2296955/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:6953258/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  1:10843430/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:11259268/1‑140 (MQ=255)

                                                                                                                             tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTGGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc           >  2:2019947/1‑140 (MQ=255)

                                                                                                                               aCCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcag         >  1:5036188/1‑140 (MQ=255)

                                                                                                                               aCCCAAAGCCGGAACCATGACCACCCGATATGGGCGTGGTCCAGTTATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTTATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcag         <  1:7224012/140‑1 (MQ=255)

                                                                                                                                 ccAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcagca       <  1:8706206/140‑1 (MQ=255)

                                                                                                                                 ccAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGAGATGTTGTGGTCGACGGGGGTTATGCTGTTTGGTTTACTGATGCCCTTATTCAGCTCGCGGTGGCTGATCTTTGCCGGAATGGTACTCTTCTcagca       <  2:9856924/140‑1 (MQ=255)

TGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCAGGATT  >  NZ_CP025268/1675262‑1675535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`