Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,731,441 A→G *200* (TAA→TAG)  nemR → DNA‑binding transcriptional regulator NemR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,731,4410AG99.8% 2147.3 / NA 616*200* (TAA→TAG) nemRDNA‑binding transcriptional regulator NemR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (275/339);  minor base T (1/0);  total (276/340)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 616 total aligned reads displayed.

GAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTATTGAAC  >  NZ_CP025268/1731304‑1731577
                                                                                                                                         |                                                                                                                                        
gAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCa                                                                                                                                        >  2:345940/1‑140 (MQ=255)
   ccGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGc                                                                                                                                     >  2:1754861/1‑140 (MQ=255)
   ccGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGc                                                                                                                                     >  1:10616614/1‑140 (MQ=255)
   ccGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGc                                                                                                                                     >  1:816086/1‑140 (MQ=255)
    cGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCTGGCAAAAATTTCGCGCATTTTCGAGCCAATGGAAAACGCGCTTGCACATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCa                                                                                                                                    <  1:2067452/140‑1 (MQ=255)
      cTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAtt                                                                                                                                  >  2:5109136/1‑140 (MQ=255)
         cAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCAttttt                                                                                                                               <  1:4926677/140‑1 (MQ=255)
            cAGGCTCAAGTGCTTGACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAATAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTAt                                                                                                                            <  2:1416022/140‑1 (MQ=255)
            cAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTAt                                                                                                                            <  2:4207365/140‑1 (MQ=255)
            cAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTAt                                                                                                                            <  1:11565271/140‑1 (MQ=255)
              ggCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCa                                                                                                                          <  2:7089089/140‑1 (MQ=255)
                cacaAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCAcc                                                                                                                        >  2:52499/1‑140 (MQ=255)
                 acaAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCTCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCa                                                                                                                       >  2:249790/1‑140 (MQ=255)
                 acaAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCa                                                                                                                       >  1:2563499/1‑140 (MQ=255)
                 acaAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCa                                                                                                                       >  2:9305735/1‑140 (MQ=255)
                   aaGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAATCAACAGTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAga                                                                                                                     >  1:4192148/1‑140 (MQ=255)
                        cTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGAcc                                                                                                                >  1:10276510/1‑140 (MQ=255)
                          ttACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCgg                                                                                                              <  2:2983725/140‑1 (MQ=255)
                          ttACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCgg                                                                                                              >  2:3669243/1‑140 (MQ=255)
                          ttACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTGTCGAGCCACTGG‑AAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCggg                                                                                                             <  2:4480079/140‑1 (MQ=255)
                           tACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCggg                                                                                                             <  1:8382252/140‑1 (MQ=255)
                           tACGCACTGGGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACCTTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCggg                                                                                                             <  1:6059009/140‑1 (MQ=255)
                           cccGCACTGGGGCGAGGCGCGAATCTGCAGGCCAATAGTTCGCTCAGTTTCGAGCCACTGGAAAACGCGCTGTCCCATGTAAAAACCAGTATTGCGACTCCTGCCGTTTCGCAGGCATTTTTTATCACCAGACGACCggg                                                                                                             <  2:11116665/138‑1 (MQ=255)
                            aCGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGa                                                                                                            <  1:8058468/140‑1 (MQ=255)
                             cGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAg                                                                                                           >  2:3242883/1‑140 (MQ=255)
                                aCTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGa                                                                                                            <  1:9934705/136‑1 (MQ=255)
                                aCTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCt                                                                                                        >  2:5566767/1‑140 (MQ=255)
                                 cTGGGGCTTGGCGCGAATCTGCCGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTACTAAACATTATTGCGACGCCTGCCGTTTAGCAGGCCTTTTTTATCACCAGACGACCGGGAGCCtg                                                                                                       >  2:5761105/1‑139 (MQ=255)
                                  tgtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCttt                                                                                                      >  1:799252/1‑140 (MQ=255)
                                  tgtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCttt                                                                                                      >  2:3931484/1‑140 (MQ=255)
                                  tgtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCttt                                                                                                      >  1:2842823/1‑140 (MQ=255)
                                  tgtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCtat                                                                                                      >  2:9203157/1‑138 (MQ=255)
                                   gtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTa                                                                                                     >  2:11537419/1‑140 (MQ=255)
                                   gtgGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTa                                                                                                     >  2:9759361/1‑140 (MQ=255)
                                       cTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTc                                                                                                 <  1:1117038/140‑1 (MQ=255)
                                       cTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTc                                                                                                 <  1:10906579/140‑1 (MQ=255)
                                         ttgcgcGAATCTGCAGGCCAATATGTCGCGCAGTTTCGCTCCACTGGAAAACGCGCTGGCCCATGTAAAAAACAGTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCAt                                                                                               <  2:10912324/138‑1 (MQ=255)
                                          ggCGCGAAACTGCAGGCCCAAATTTCGCGCAGTTTCGAGCCACGGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCTACGCCTGCCGTTTAGCAGGCCTTTTTTATCACCAGACGACCGGGAGCCTGTATGTCATc                                                                                              >  2:2304153/1‑140 (MQ=255)
                                            cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                            >  2:6926409/1‑140 (MQ=255)
                                            cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                            >  2:9922807/1‑140 (MQ=255)
                                            cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                            >  1:1438322/1‑140 (MQ=255)
                                            cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                            >  1:3327992/1‑140 (MQ=255)
                                              cgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaa                                                                                          >  1:307058/1‑140 (MQ=255)
                                               gAATCTGCAGGCCTAAATTTCGCGCAGTTTCGAGCCACTGGACAACGCGCTGGCCCCTGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTGATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaa                                                                                         <  2:9015047/140‑1 (MQ=255)
                                                aaTCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaaa                                                                                        <  1:2390764/140‑1 (MQ=255)
                                                aaTCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaaa                                                                                        >  1:6849260/1‑140 (MQ=255)
                                                         gCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTTAAAAACTGTATTc                                                                               <  1:10018371/140‑1 (MQ=255)
                                                         gCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                               <  2:4296354/140‑1 (MQ=255)
                                                         gCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                               <  2:2680264/140‑1 (MQ=255)
                                                         gCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                               <  1:6591842/140‑1 (MQ=255)
                                                           cAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                             >  1:10460543/1‑140 (MQ=255)
                                                           cAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTccc                                                                             <  2:7616581/140‑1 (MQ=255)
                                                           cAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTGTATGTCATCTGAAAAACTGTATTccc                                                                             >  1:9761174/1‑140 (MQ=255)
                                                                     cgcAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGt                                                                   <  2:10630962/140‑1 (MQ=255)
                                                                        aGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTggg                                                                <  1:4518704/140‑1 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCCTGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcggc                                                    >  1:5193741/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcggc                                                    >  1:3528385/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcggc                                                    >  2:4759179/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcggc                                                    >  1:547546/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACGGTATTCCCCACTGACTGTGGGCGCGATCAcggc                                                    >  1:11074224/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCAGCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcggc                                                    >  1:6989774/1‑140 (MQ=255)
                                                                                    cTGGAAAACGCGCTGGCCCATGTAAAAAACAGTATTGCGACGCCTGCCGGTTAGCAGGCATTTTTTATCACCAGACGACAGGGAGCCTTGATGTCATCTGAAAAACTGTATTCCCCACGGAAAGTGGGCGCGATCAcggc                                                    >  2:641419/1‑140 (MQ=255)
                                                                                       gAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                                 <  2:6661341/140‑1 (MQ=255)
                                                                                        aaaaCGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCa                                                <  2:8619388/140‑1 (MQ=255)
                                                                                          aaCGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCaaa                                              <  1:7304492/140‑1 (MQ=255)
                                                                                           aCGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAAc                                             <  1:9305736/140‑1 (MQ=255)
                                                                                             gcgcTGGCCCATGTAAAAAACATTAGTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCg                                           <  1:1563336/140‑1 (MQ=255)
                                                                                               gcTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGTCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTa                                         <  2:6860918/140‑1 (MQ=255)
                                                                                                 tGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAATACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTAtt                                       >  2:6079067/1‑140 (MQ=255)
                                                                                                     ccATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTa                                   <  1:4845395/140‑1 (MQ=255)
                                                                                                        tGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATgg                                <  1:1862294/140‑1 (MQ=255)
                                                                                                             aaaaCATTATTGCGACGCCTGCCGTTTAGCAGGGATTTTTTATCACCAGACGACCGGGAGCCTTTATGGACGCTGAAGTACTGTTTTGCCCAGTGAACGGGGGGGCGTTCACGGCGGCAAAGCGGATTTTTATGGCAcgg                           >  2:8353201/1‑138 (MQ=255)
                                                                                                             aaaaCATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCg                           >  2:7787896/1‑140 (MQ=255)
                                                                                                             aaaaCATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCg                           >  1:9582194/1‑140 (MQ=255)
                                                                                                                  attattGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAc                      >  1:2235877/1‑140 (MQ=255)
                                                                                                                  attattGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAc                      >  1:7621597/1‑140 (MQ=255)
                                                                                                                  attattGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAc                      >  1:4070843/1‑140 (MQ=255)
                                                                                                                  attattGCGACGCCAGCCGTTTAGCAGGCATTTTGTATCACCAGACGACCGGGAGCCTGTCAGGCAGCTGTCTTACTGTATTCGCCACTGTACGTGGGCGCGCTCTCGGCGGCACACGGGAGGTGTATGGGACCggggtg                      >  2:10295125/1‑135 (MQ=255)
                                                                                                                      ttGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGt                  >  2:7261081/1‑140 (MQ=255)
                                                                                                                      ttGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGt                  <  1:5626728/140‑1 (MQ=255)
                                                                                                                      ttGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGt                  >  2:10781192/1‑140 (MQ=255)
                                                                                                                         cGACGCCTGCCGTTGAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTg               <  2:1137235/140‑1 (MQ=255)
                                                                                                                           ccGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:7442316/139‑1 (MQ=255)
                                                                                                                           ccGCCAGCCGTGTAGCAGGCATATTTTATCACCAGCCGACCGGGAGCCTTGATGTCATCTGAAAAACTGTATACCCCCCTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  2:3519182/139‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:8274582/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:8552571/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  2:9582193/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  2:9425362/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:8761350/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:1138227/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:8963457/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             >  2:7924739/1‑140 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:6338493/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  2:2138040/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:1259139/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  2:2842823/140‑1 (MQ=255)
                                                                                                                           aCGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTCTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg             <  1:3868438/140‑1 (MQ=255)
                                                                                                                            cGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcgc            <  1:5771138/140‑1 (MQ=255)
                                                                                                                              ccTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAg          >  2:10112348/1‑140 (MQ=255)
                                                                                                                                 gCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTAt       >  1:9078459/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GAACCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCGCAGTATTGAAC  >  NZ_CP025268/1731304‑1731577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑