BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,002,534	T→C	329 (TAA→TAG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,002,534	0	T	C	99.6%	2488.7 / NA	717	329 (TAA→TAG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (362/352); minor base A (3/0); total (365/352)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 717 total aligned reads displayed.

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                        >  1:6378963/1‑140 (MQ=255)

   gagaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc                                                                                                                                     >  1:3542219/1‑140 (MQ=255)

    agcaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACg                                                                                                                                    <  2:8517374/137‑1 (MQ=255)

      aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  <  1:601076/140‑1 (MQ=255)

      aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  <  2:4209754/140‑1 (MQ=255)

      aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  <  1:2814246/140‑1 (MQ=255)

         aaaCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgggg                                                                                                                               <  2:7501514/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                             <  1:8497420/140‑1 (MQ=255)

             ccGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgatga                                                                                                                           <  2:9315388/140‑1 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTCAACGTGGGGATGATAg                                                                                                                        >  1:133141/1‑140 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                        >  1:462183/1‑140 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  2:4655369/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  1:1472206/140‑1 (MQ=255)

                      aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAc                                                                                                                  <  1:811634/140‑1 (MQ=255)

                       aGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCGTTCAGCAGGAACGGCAAGGAATCAATAACCCGGTGTCGCCTTTCTTGCTTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACa                                                                                                                 >  2:1920299/1‑140 (MQ=255)

                                aCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGgcg                                                                                                        <  2:534457/140‑1 (MQ=255)

                                 cATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGgcgc                                                                                                       <  1:10099391/140‑1 (MQ=255)

                                   tGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTGAACGTGGGGATGATAGGCGAAc                                                                                                                  <  2:11411886/127‑1 (MQ=255)

                                   tGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAc                                                                                                     >  1:11279749/1‑140 (MQ=255)

                                     ccgccgATACTGCGTTGCAGCGTAGACCCAGCCCCTTTCCTCAGGAACTGACAGGAATCAATAACCAGTTGTATACTTTCTTGCAGTAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCTAACAGCGAAGGCGAAcag                                                                                                   <  2:559806/140‑3 (MQ=255)

                                         cgctACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:4363864/137‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  1:7176374/140‑1 (MQ=255)

                                          gATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCATGATCGGCAAGGAATCAATAACCAGTTGTAAACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGt                                                                                              <  2:8384379/140‑1 (MQ=255)

                                          gATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGt                                                                                              <  1:6752690/140‑1 (MQ=255)

                                           aTACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATCACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                             <  2:6559459/140‑1 (MQ=255)

                                              cTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                          <  2:3404769/140‑1 (MQ=255)

                                                gAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAAt                                                                                        >  1:315006/1‑140 (MQ=255)

                                                 aGTTGCAGCGTATACCCAGCCCCTTACAGCAGGAACGGCAAGGAAACAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGAGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTAAGAATa                                                                                       >  1:10595538/1‑140 (MQ=255)

                                                   ttGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaaa                                                                                     <  1:6220755/140‑1 (MQ=255)

                                                     gCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCCGTTGGATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTCTGAATAAAcc                                                                                   >  2:10967579/1‑139 (MQ=255)

                                                     gCAGCGTATACCCAGCCCCTATCAGCAGGAACGGCAAGGAATCAATAAACAGTTGTATACTTACTTGCATGATCGTTTCCGGAGCTAAACGAGGTGATGAGAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACa                                                                                   >  2:3869949/1‑140 (MQ=255)

                                                          gTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATc                                                                              <  2:6061851/140‑1 (MQ=255)

                                                           tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                             >  1:1990817/1‑140 (MQ=255)

                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGc                                                                           >  2:5446369/1‑140 (MQ=255)

                                                              aCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGcc                                                                          >  2:7305166/1‑140 (MQ=255)

                                                                ccAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAATGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCt                                                                        >  2:1439520/1‑140 (MQ=255)

                                                                 cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                       <  1:535938/140‑1 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  2:2511020/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:8820372/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  2:118695/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:6596508/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:2463839/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:331607/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:4338168/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  1:5237254/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                     >  2:4932784/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  2:1326995/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  1:6952550/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  1:8970867/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                    >  1:9457808/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGACTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  2:1854843/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                   >  2:3006780/1‑140 (MQ=255)

                                                                       cTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCtt                                                                 >  1:9704430/1‑140 (MQ=255)

                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCttt                                                                >  1:8289184/1‑140 (MQ=255)

                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                               >  1:7654301/1‑140 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  1:2367180/140‑1 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCGTCTTTGAAg                                                            <  1:8694951/140‑1 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAg                                                            <  1:10000746/140‑1 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAg                                                            <  1:7553183/140‑1 (MQ=255)

                                                                                 gAACGGCAAGGAATCAATCACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                       <  2:4768756/140‑1 (MQ=255)

                                                                                 gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                       >  1:4071990/1‑140 (MQ=255)

                                                                                 gAAAGGCAAGGAATCAATAACAAGTTGTATACATTATTGCATGATCGATACCGGAGCTCAACGTGGTGATGAACGGCGAACAGCGCAGGCGCACCGCCGGTATGAATCTACAGAATCGGACCTGCCTCTGTGACGCGatt                                                       >  2:9459518/1‑140 (MQ=255)

                                                                                     ggCACGGAATCACTAACCAGTGGAATACTTTCATGCAAGAGCGTTTCCCGAGCTAAACGTGGGGATGAACGGCGAACAGCGCAGGCGCACCGCCGGTAGGAATAAACAGAACCGGCCCTACATCTATGCAGCGATTctga                                                   <  2:5934865/140‑1 (MQ=255)

                                                                                             aTCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATAcc                                           <  2:1679118/140‑1 (MQ=255)

                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAg                                  >  1:224061/1‑140 (MQ=255)

                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAg                                  >  2:7058932/1‑140 (MQ=255)

                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAg                                  >  2:321653/1‑140 (MQ=255)

                                                                                                       aGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGc                                 <  1:9793950/140‑1 (MQ=255)

                                                                                                       aGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGc                                 <  1:11507072/140‑1 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                                >  1:7445833/1‑140 (MQ=255)

                                                                                                         ttGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGccc                               >  1:10998132/1‑140 (MQ=255)

                                                                                                          tGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcccc                              >  1:4206237/1‑140 (MQ=255)

                                                                                                          tGTATAATTTCTTGCATGAGCGTTTCCGGAGCTACACGTGGGGATGAAAGGAGAACAGCTCAGGCGCACCTCCGGTATGAATAAAAAGAAACGGCCCGTCATCTGTGAAGCGTTTCTGACTGAAACCGTCAATCAGaccc                              >  2:2043390/1‑140 (MQ=255)

                                                                                                           gTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCg                             >  2:8144757/1‑140 (MQ=255)

                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGc                            >  2:9574694/1‑140 (MQ=255)

                                                                                                            tataCATTCTAGCAAGAGCCTATCCCGAGCTAAACGAGGGGATGATAGCCCAACAGCGCAGCCGCACCCCCGGAATGAATAAACAGAATCGGCCCATCATCATTGAAGCGTTTCTAACTGATACCTTAAAACAGCCACGc                            >  2:10374826/1‑140 (MQ=255)

                                                                                                                cTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc                        >  1:1029538/1‑140 (MQ=255)

                                                                                                                cTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc                        >  1:7855019/1‑140 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                       <  1:1916980/140‑1 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGTCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:3634930/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  1:5554513/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:6267468/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:5149910/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCGTCATCGTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAGCGCt                     >  1:4759835/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTGTCCGGAGCAAAACGGGGGGATGATCGGCGAACAGCGCAGGCGCACCGCCGGTATGACTAAAAAGAATCGGCCCTGCATCTTTGAAGCGGTTCTGACTGATACCGTCAATCCGCCCCGCCATCGCt                     >  2:10605463/1‑140 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  1:10456452/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  1:1758577/1‑140 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  1:995982/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  2:7678980/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  2:8834499/1‑140 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  2:8038931/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAAACGGCCCTTCAACTTTCAAGCGATTCTCACTGATACCGACAATCACCCCCGCCATCGCattt                  <  2:4077907/140‑1 (MQ=255)

                                                                                                                       gCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                 <  2:230858/140‑1 (MQ=255)

                                                                                                                       gCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                 <  2:7819990/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCGTCTTTGAAGCGTTTCTGACTGATACCGTCAAGCAGCCCCGCCATCGCTTTTCCggg             >  2:6857921/1‑139 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:3731989/140‑1 (MQ=255)

                                                                                                                            aGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGAATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGta            >  1:107335/1‑140 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca        <  2:9095860/140‑1 (MQ=255)

                                                                                                                                   ccGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAg     >  2:10168704/1‑140 (MQ=255)

                                                                                                                                      gAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAt  >  1:5021343/1‑140 (MQ=255)

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`