BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.5%	2204.0 / NA	637	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (296/338); minor base A (1/2); total (297/340)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 637 total aligned reads displayed.

ATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414951‑2415222

  cAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCa                                                                                                                                    >  1:3532712/1‑140 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCATACGTGACGGCGACTTATGCATACGCATTGCAACGCTTAACACCACGGGAATAAAGATAGAGACAACGCCTGAAGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  2:5239927/1‑140 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:5185033/1‑140 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 <  2:6064609/140‑1 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  2:129260/1‑140 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:2584194/1‑140 (MQ=255)

     cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGGTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 >  1:6774982/1‑140 (MQ=255)

             aaCTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAAcc                                                                                                                         <  1:6964507/140‑1 (MQ=255)

                       catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCTACGCTCAGCATCAACAAAACCAACATTTTGa                                                                                                               >  1:8362008/1‑140 (MQ=255)

                        atcatcAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAt                                                                                                              <  1:7304371/140‑1 (MQ=255)

                         tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGAtt                                                                                                             <  1:9405711/140‑1 (MQ=255)

                            tcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCa                                                                                                          <  1:1823672/140‑1 (MQ=255)

                            tcaGACGTGACGGCGACGTGTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCa                                                                                                          <  1:9945876/140‑1 (MQ=255)

                               gACGTGACGGCGACGTTGGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTa                                                                                                       <  2:9030324/140‑1 (MQ=255)

                                 cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACTCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTAtt                                                                                                     >  2:419289/1‑140 (MQ=255)

                                 cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACCTTTTGATTTCACTAtt                                                                                                     >  1:3815836/1‑140 (MQ=255)

                                     aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGccc                                                                                                 <  1:3140623/140‑1 (MQ=255)

                                      cGGCTACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGTTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  2:10862383/140‑1 (MQ=255)

                                      cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:2676109/140‑1 (MQ=255)

                                      cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCg                                                                                                <  1:11444033/140‑1 (MQ=255)

                                         cGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCCAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCaa                                                                                             <  1:7183227/140‑1 (MQ=255)

                                          gACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAAc                                                                                            >  1:7805611/1‑140 (MQ=255)

                                                tGCATTCGCATGGGCTCGCGTAACCCCACGGGAATAATGATAGCGACATCGCCGGTTGAACTCTTAATCGCGCCAAGCCCCGCGTCGCTCAGCATCATCACAACCACCATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  2:9648770/140‑1 (MQ=255)

                                                tGCATACGCATGGCAACGCTTCACACCACGGGAAGAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  2:7783505/140‑1 (MQ=255)

                                                tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  1:7502685/140‑1 (MQ=255)

                                                tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCAGCGACGCTCAGCATCAACAAAACCAACATTTTTATTTCACTATTGCCCGCAACTTTGAc                                                                                      <  1:10380270/140‑1 (MQ=255)

                                                 gCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAcc                                                                                     <  2:11425418/140‑1 (MQ=255)

                                                     aCGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAACCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGcc                                                                                 <  2:7060179/140‑1 (MQ=255)

                                                       tcATGGAAACGCTTAACACCACGGGAAAAAAGATAGCGACAACGCCTGATGAACTCATAAACGCGCCAAGCCCCGAGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACAATAGCCCGCAACATTGACCAGACAt                                                                               >  2:5080907/2‑140 (MQ=255)

                                                        cATGGCAACGCTTAACACCACGGGAATACAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATg                                                                              <  2:6653937/140‑1 (MQ=255)

                                                         aTGGCATCGCTTCCCACCACGGGAAGAAAGATTGCGTCAACGCCGGTTGAACTCATAAACGCGCCAAGCCACGCGACGCTCAGCATCTAAAAAACCAACGTTTTGATTTCACTTTTGCCCGGAACTTTGACCAGCCATGt                                                                             <  2:3794678/140‑1 (MQ=255)

                                                         aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                             <  1:10576110/140‑1 (MQ=255)

                                                          tGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGtt                                                                            >  1:1239433/1‑140 (MQ=255)

                                                          tGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGtt                                                                            >  1:4409995/1‑140 (MQ=255)

                                                           ggCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTc                                                                           >  1:5504870/1‑140 (MQ=255)

                                                            gCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTcc                                                                          >  1:5911713/1‑140 (MQ=255)

                                                                cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                      >  1:9586913/1‑140 (MQ=255)

                                                                 gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAGTa                                                                     >  2:6355874/1‑140 (MQ=255)

                                                                  cTTAACACCACGGGCATAAAGCTAGCGACAACGCCTGTTGAACTCCTAAACGCGCCTAGCCCCGCGACGCTCAGCATCAACAAAACCAACAATTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    <  2:2743401/140‑1 (MQ=255)

                                                                  cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                    >  2:11262962/1‑140 (MQ=255)

                                                                   ttAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACg                                                                   <  1:10473215/140‑1 (MQ=255)

                                                                   ttAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACg                                                                   <  1:10089153/140‑1 (MQ=255)

                                                                   ttAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACg                                                                   <  1:877112/140‑1 (MQ=255)

                                                                     aaCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGt                                                                 >  2:6857658/1‑140 (MQ=255)

                                                                        accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  2:5411192/140‑1 (MQ=255)

                                                                        accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                              <  1:2290908/140‑1 (MQ=255)

                                                                          cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:1934707/1‑140 (MQ=255)

                                                                          cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:2248949/1‑140 (MQ=255)

                                                                          cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  2:5510643/1‑140 (MQ=255)

                                                                          cacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCaa                                                            >  1:4023960/1‑140 (MQ=255)

                                                                           acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                           >  1:2056546/1‑140 (MQ=255)

                                                                            cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:9233145/1‑140 (MQ=255)

                                                                            cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:6209373/1‑140 (MQ=255)

                                                                            cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  2:3458982/1‑140 (MQ=255)

                                                                            cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                          >  1:4539641/1‑140 (MQ=255)

                                                                             gggAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAacac                                                         >  1:1457591/1‑140 (MQ=255)

                                                                               gAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCg                                                       >  1:2872310/1‑140 (MQ=255)

                                                                                 aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  1:7954183/140‑1 (MQ=255)

                                                                                 aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                     <  2:6533927/140‑1 (MQ=255)

                                                                                  tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCTCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:1762051/140‑1 (MQ=255)

                                                                                  tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                    <  2:4345753/140‑1 (MQ=255)

                                                                                    aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACg                                                  <  1:11229424/140‑1 (MQ=255)

                                                                                       aTAGCGACAACGCCGGTTGTACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAc                                               >  1:11566741/1‑140 (MQ=255)

                                                                                        tAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGAcc                                              <  2:5727092/140‑1 (MQ=255)

                                                                                           cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  1:8980482/1‑140 (MQ=255)

                                                                                           cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                           >  1:8186065/1‑140 (MQ=255)

                                                                                             aCAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAAcc                                         >  1:9763695/1‑140 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAATCCCCGCGACGCTCAGCATCAACAAAACCAACATATTGATTTCACTATTTCCCGCAACTTGGACCAGCCATGTTCCCATGACGGTGGCAACACCGGTACGGACCAAACCCTCGc                                    <  2:1607797/140‑1 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:1156637/140‑1 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:7229497/140‑1 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  1:5259732/140‑1 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:1029059/140‑1 (MQ=255)

                                                                                                  gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                    <  2:11018556/140‑1 (MQ=255)

                                                                                                      gTTTAACTCATAAACGCGCCAAGCCCCGCGACGCTCTGCATCAACAAATCCAACTGTTTGATTTGACTATTGCCCGCAACTTTGACAAGCCATGTTCACAGAACGGTGGCAACACCGGAACGGACCAAACCATCGACaat                                >  2:3107133/1‑140 (MQ=255)

                                                                                                      gTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaat                                >  2:8561036/1‑140 (MQ=255)

                                                                                                                 aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                     >  2:10616656/1‑140 (MQ=255)

                                                                                                                 aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                     <  1:6504337/140‑1 (MQ=255)

                                                                                                                 aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                     >  1:4785733/1‑140 (MQ=255)

                                                                                                                   aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  2:4855404/140‑1 (MQ=255)

                                                                                                                   aCGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                   <  1:7717341/140‑1 (MQ=255)

                                                                                                                      cgcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCCTCGCCACTAATAAACACGGCGGCa                >  2:7582872/1‑140 (MQ=255)

                                                                                                                      cgcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCa                >  2:8484380/1‑140 (MQ=255)

                                                                                                                       gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa               >  2:2597324/1‑140 (MQ=255)

                                                                                                                       gcCAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa               >  2:8909684/1‑140 (MQ=255)

                                                                                                                         cAAGCCCCGCGACGCTCAGCATCCACAAAACCAACATTTTGATTTCACTATTGCCCTCAACTTTGACCAGCCATGTTCCCCTTACGGTGGCCACACCGGTACGGACCAAACCATCGCCAATAAGACACACGGCGGCACTc             >  2:7461816/1‑140 (MQ=255)

                                                                                                                         cAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATc             >  1:10948620/1‑140 (MQ=255)

                                                                                                                         cAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATc             >  1:5766319/1‑140 (MQ=255)

                                                                                                                         cAAGCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATATCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCACCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATc             >  2:4553381/1‑140 (MQ=255)

                                                                                                                            gCCCCGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAgg          <  1:7094614/140‑1 (MQ=255)

                                                                                                                             ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:8243307/140‑1 (MQ=255)

                                                                                                                             ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  2:9755198/140‑1 (MQ=255)

                                                                                                                             ccccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa         <  1:8483662/140‑1 (MQ=255)

                                                                                                                              cccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc        <  1:3323968/140‑1 (MQ=255)

                                                                                                                               ccGCGACGCTCAGCATCAACAAACCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  1:1607847/140‑1 (MQ=255)

                                                                                                                               ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  1:2983508/140‑1 (MQ=255)

                                                                                                                               ccGCGACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa       <  1:4935969/140‑1 (MQ=255)

                                                                                                                                 gcgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAAc     >  1:5592346/1‑140 (MQ=255)

                                                                                                                                 gcgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAAc     >  2:94456/1‑140 (MQ=255)

                                                                                                                                  cgACGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACg    <  1:10850643/140‑1 (MQ=255)

                                                                                                                                    aCGCTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGtt  <  1:1351630/140‑1 (MQ=255)

ATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAACAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTT  >  NZ_CP025268/2414951‑2415222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`