BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,622,099	T→C	234 (TAA→TAG)	hda ←	DnaA inactivator Hda

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,622,099	0	T	C	99.9%	2419.4 / NA	698	234 (TAA→TAG)	hda	DnaA inactivator Hda
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (357/340); minor base G (0/1); total (357/341)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 698 total aligned reads displayed.

GATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621962‑2622235

gATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTa                                                                                                                                        <  2:5619537/140‑1 (MQ=255)

   gtgCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAANACCTTATCTACaa                                                                                                                                     <  1:10006916/140‑1 (MQ=255)

    tgCTCCATCGCTAGTTTAAGCACATTGCCGGATGCGACGCTTGCCACGTATTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAAc                                                                                                                                    <  2:1597140/140‑1 (MQ=255)

      cTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACtt                                                                                                                                  >  1:8147184/1‑140 (MQ=255)

              cTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAttt                                                                                                                          >  1:11301403/1‑140 (MQ=255)

              cTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAttt                                                                                                                          >  1:383864/1‑140 (MQ=255)

                     aaGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTcac                                                                                                                   <  1:958145/140‑1 (MQ=255)

                      aGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTcaca                                                                                                                  <  2:7084506/140‑1 (MQ=255)

                          caTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGACTACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACg                                                                                                              <  2:725045/140‑1 (MQ=255)

                            ttGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAACCGGa                                                                                                            <  2:4130461/140‑1 (MQ=255)

                            ttGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGa                                                                                                            <  1:7578642/140‑1 (MQ=255)

                            ttGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGa                                                                                                            <  1:4257578/140‑1 (MQ=255)

                            ttGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACATCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACAACAGAAATTCTTTCACAAACGGa                                                                                                            <  2:2850118/140‑1 (MQ=39)

                             tGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGaa                                                                                                           >  1:6348090/1‑140 (MQ=255)

                               tcGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTTTGGATTAACTTCGCGGAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                         >  2:4403504/2‑140 (MQ=255)

                               ccGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                         >  1:6574071/1‑140 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTCCGAATACTATGGATGAACGTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgt                                                                                                        <  2:2769282/140‑2 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:1433591/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:778425/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:2117073/140‑1 (MQ=255)

                                cGGATGCGACGCTTGCCACGTCTGATCCGGCCTACGAATACTATGTATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:10808080/140‑1 (MQ=255)

                                  gATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTc                                                                                                      <  1:5843570/140‑1 (MQ=255)

                                            ttGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGtt                                                                                            <  1:5241863/140‑1 (MQ=255)

                                            ttGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCGTACGtt                                                                                            >  2:5225144/1‑140 (MQ=255)

                                             tGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCATCTTACGTTg                                                                                           >  2:347025/1‑140 (MQ=255)

                                                   gTCTTATCCGGCCTACGAATACTTTGGCTTAACTTCGCGTCGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATACTCACCTAATCTACATCTTCAGAATTTCTGTGAGAATCGGAGTGGTCAGATTACGTTGCGCGGt                                                                                     >  2:8055595/1‑140 (MQ=255)

                                                      ttATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACACTAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                                  >  2:6440134/1‑140 (MQ=255)

                                                      ttATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                                  >  1:1143233/1‑140 (MQ=255)

                                                        aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCTCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                <  2:2647431/140‑1 (MQ=255)

                                                        aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                >  2:5831721/1‑140 (MQ=255)

                                                         accagCCTACGAAAACTAAGGATTAACAACTCCAAGATCGGATAACGCGTTCGCGCCGCATCCGACAATAAACACCAAATCTACAACTTCAGAATTTCTATCACACACGGAATGCTCAGCTTACGTTTCGCGGTAAACGa                                                                               >  2:6210724/5‑140 (MQ=255)

                                                           cGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                             <  1:6237276/140‑1 (MQ=255)

                                                           cGGCCTACGAATACTATGGATGAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                             <  2:11553457/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:6833718/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:1528608/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:6339914/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:391977/140‑1 (MQ=255)

                                                             gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:4734050/140‑1 (MQ=255)

                                                             gCCTACGAAGACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:1166991/140‑1 (MQ=255)

                                                             gCCTACGAAAACTATGGATTAAATTCGCGTAGTTCGGATAATGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGTATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  2:8830047/140‑1 (MQ=255)

                                                              ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATATCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:9461119/1‑140 (MQ=255)

                                                               cTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACg                                                                         >  1:607936/1‑140 (MQ=255)

                                                                      tACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAAc                                                                  <  1:10858147/140‑1 (MQ=255)

                                                                        cgaTGGAGTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  2:3072086/3‑140 (MQ=255)

                                                                        cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  1:7369338/1‑140 (MQ=255)

                                                                        cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  2:10537548/1‑140 (MQ=255)

                                                                           tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  1:8539036/1‑140 (MQ=255)

                                                                            ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  2:9011070/1‑140 (MQ=255)

                                                                             gATTCACTTCGCGTAGTTCGGATACGGCGATCGCGCCGCATCCGACACGACACACCTCATCGCCAACGTCAGACGTTCTGTCACCCCCGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCACCTGATCCa                                                           <  2:817647/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:7089295/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:2303372/140‑1 (MQ=255)

                                                                             gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  1:6713371/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:3598917/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:1943447/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:4038085/140‑1 (MQ=255)

                                                                               ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:1763049/140‑1 (MQ=255)

                                                                               taaaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:10527247/138‑1 (MQ=255)

                                                                                 aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  2:3330436/140‑1 (MQ=255)

                                                                                   cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  1:5448950/1‑140 (MQ=255)

                                                                                   cTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCa                                                     >  2:2115546/1‑140 (MQ=255)

                                                                                     tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTTGAACTTCAGAATTTCTTTCACCAACGGAATGGTCAGCTTTCGTTGCGCGGTTATCGACGCACGATCCAACTGATCCAACGTCATa                                                   >  2:4039771/1‑140 (MQ=39)

                                                                                     tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACATTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCTACGCACGATCCAACTGATCCAACGTCATa                                                   >  1:3361155/1‑140 (MQ=255)

                                                                                      agcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaa                                                  <  2:11301402/139‑1 (MQ=255)

                                                                                       gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTGCTATCACAAACGGACTGGTCAGCTAACGGTGCGCGGTAATCGACGCACGATCCAGCGGATCCCACGTCagaca                                                 >  2:8578345/1‑136 (MQ=38)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  2:11097730/1‑140 (MQ=255)

                                                                                        cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  1:10716185/1‑140 (MQ=255)

                                                                                           aGTTCGGATAAGGCTTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGATTGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCTACGTCATAAATAGc                                             <  1:2907665/140‑1 (MQ=255)

                                                                                           aGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGc                                             <  2:9331046/140‑1 (MQ=255)

                                                                                           aGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGc                                             <  2:1308547/140‑1 (MQ=255)

                                                                                             ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAATTAGCGt                                           >  2:6661456/1‑140 (MQ=255)

                                                                                             ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           <  1:9464924/140‑1 (MQ=255)

                                                                                             ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           >  1:4553191/1‑140 (MQ=255)

                                                                                             ttCGGATAAGGCGTTCGCGCCGCATCCGACAAGAAACACCTTATCTACAACTTCCGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAGCCAACGGCGTAATTCGCGt                                           >  2:6000274/1‑140 (MQ=255)

                                                                                             ttCCGATAAGGCGTTCGCGCCGCAACCGACAATACACACCTTATCTACAACTTCCGAATTTCTTTCACAAACGGAAGGGTCAGCTTACGTTGCGCGGTACTCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           <  2:7862928/140‑1 (MQ=255)

                                                                                                ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  2:4607981/140‑1 (MQ=255)

                                                                                                ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  1:2770368/140‑1 (MQ=255)

                                                                                                       gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  1:11014084/1‑140 (MQ=255)

                                                                                                       gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  1:4983413/1‑140 (MQ=255)

                                                                                                           tCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGt                             >  1:1609092/1‑140 (MQ=255)

                                                                                                            cgcgCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTc                            >  1:2442658/1‑140 (MQ=255)

                                                                                                            cgcgCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTc                            >  2:3702641/1‑140 (MQ=255)

                                                                                                                 cgcATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGcc                       >  1:3079569/1‑140 (MQ=255)

                                                                                                                   cATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGc                     <  2:4898244/140‑1 (MQ=255)

                                                                                                                    aTCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCt                    >  1:4468127/1‑140 (MQ=255)

                                                                                                                        gACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCACTGTCGAGCCGCTTCAg                >  2:10326460/1‑140 (MQ=255)

                                                                                                                         aCAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGc               <  1:8397443/140‑1 (MQ=255)

                                                                                                                             tAAACACCTTATCTACAAGTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGa           >  2:971233/1‑140 (MQ=255)

                                                                                                                             tAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           >  1:8941816/1‑92 (MQ=255)

                                                                                                                              aaaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa          <  1:2615479/140‑1 (MQ=255)

                                                                                                                              aaaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaa          <  2:8370751/140‑1 (MQ=255)

                                                                                                                               aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  1:10372716/140‑1 (MQ=255)

                                                                                                                               aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:5423534/140‑1 (MQ=255)

                                                                                                                                acacCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAAc        <  2:1880035/140‑1 (MQ=255)

                                                                                                                                    cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    >  2:7588363/1‑140 (MQ=255)

                                                                                                                                    cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    >  1:5739803/1‑140 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAACTAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  1:8004258/140‑1 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  2:10974906/140‑1 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  1:9299312/140‑1 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGAAGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  >  1:8747760/1‑140 (MQ=255)

                                                                                                                                      tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGCTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  >  2:10034931/1‑140 (MQ=255)

GATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621962‑2622235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`