BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,938,222	A→G	S112G (AGC→GGC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,938,222	0	A	G	99.8%	1914.7 / ‑5.3	557	S112G (AGC→GGC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (284/271); total (285/272)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 557 total aligned reads displayed.

CTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAG  >  NZ_CP025268/2938085‑2938357

cTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGc                                                                                                                                       <  1:9044046/140‑1 (MQ=255)

 tGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCa                                                                                                                                      <  2:10840734/140‑1 (MQ=255)

     gCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACCTGGATCCAACCCGCCCGAAGGCCAGTTGGGGCTTTAACGGCACTGa                                                                                                                                  >  2:4778735/1‑140 (MQ=255)

     gCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGa                                                                                                                                  >  1:10040025/1‑140 (MQ=255)

     gCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGa                                                                                                                                  >  1:2732511/1‑140 (MQ=255)

      ccGCTGCTTGCGAAGATAAATTCAGCAGTGAGAATGTAGGCCTAAACAGTACGGTAACGCCTTTCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCAGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                 <  2:5427445/140‑1 (MQ=255)

      ccGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGaa                                                                                                                                 <  1:7884707/140‑1 (MQ=255)

          tgctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGcc                                                                                                                             >  1:11438526/1‑140 (MQ=255)

          tgctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGcc                                                                                                                             >  2:5044571/1‑140 (MQ=255)

           gctTGCGAAGATCAATTCAGCAGTCAGAATGTAGGCCTAACCAGTACGGTAACGCCTTGCTGGTGCTATGGCTGTGAAACCATCGACATGGATCCAAACCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGccc                                                                                                                            <  2:7127652/140‑1 (MQ=255)

           gctTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGccc                                                                                                                            <  1:5451983/140‑1 (MQ=255)

             ttGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCg                                                                                                                          <  1:5760881/140‑1 (MQ=255)

                cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                       <  2:5925592/140‑1 (MQ=255)

                cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                       <  2:1463080/140‑1 (MQ=255)

                cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                       <  1:10885911/140‑1 (MQ=255)

                cGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCGTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGACGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGgcg                                                                                                                       <  2:2820922/140‑1 (MQ=255)

                     aaaaaTTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGtt                                                                                                                  <  1:2855532/140‑1 (MQ=255)

                     aaaaaTTCAGCAGTCAGAAAGTAGGCCTCACCATGACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGtt                                                                                                                  <  2:269069/140‑1 (MQ=255)

                        aagtCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTAc                                                                                                               <  1:6740906/137‑1 (MQ=255)

                                agTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGc                                                                                                       <  1:8782062/140‑1 (MQ=255)

                                agTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGc                                                                                                       <  2:2355481/140‑1 (MQ=255)

                                  tCAGAATTTAGGACTAACCATAAACTTAACGCCTTGCTAGTTCAATGGCAGAGAAACCATCGACATGGATCCAACCCGCAAGAAGGACATTTGGGGCTTTAACGTCACTGAACGCCACGGCGCTGTTTACCTGGCAGCgg                                                                                                     >  2:5176126/1‑140 (MQ=255)

                                        tGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACCTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTGTACCTGGCAGCGGCTCTgg                                                                                               <  2:2389555/140‑1 (MQ=255)

                                         gTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGc                                                                                              >  1:7157585/1‑140 (MQ=255)

                                         gTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGc                                                                                              <  2:23053/140‑1 (MQ=255)

                                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCCTTTGGGGCTGTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                           >  2:8220139/1‑140 (MQ=255)

                                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                           >  1:1757349/1‑140 (MQ=255)

                                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                           >  1:11569173/1‑140 (MQ=255)

                                            ggCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGc                                                                                           >  1:6001131/1‑140 (MQ=255)

                                              ccTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTc                                                                                         >  2:8032843/1‑140 (MQ=255)

                                               cTCACCATTACGGTAACGCCTTGCTGGTGCTGTGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTca                                                                                        <  1:11110941/140‑1 (MQ=255)

                                                tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                       <  1:7021826/140‑1 (MQ=255)

                                                tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                       >  1:325061/1‑140 (MQ=255)

                                                tCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcac                                                                                       <  1:7722364/140‑1 (MQ=255)

                                                 cACCATTAAGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCAACGGCCATTTGGGGATTTAACGGCACTGAACGCCCAGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTcaca                                                                                      <  2:643018/140‑1 (MQ=255)

                                                  aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                     >  1:7414458/1‑140 (MQ=255)

                                                  aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                     >  1:4342793/1‑140 (MQ=255)

                                                  aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                     >  1:4230357/1‑140 (MQ=255)

                                                  aCCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAg                                                                                     >  1:4043854/1‑140 (MQ=255)

                                                       tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCCTTTGGGGCTTTAACGGCACTGAACGCCCCGGCGGTGTTTACCTGGCAGCGGCTCTGGCTGCTCACAGCCAGa                                                                                >  2:11061742/1‑140 (MQ=255)

                                                       tACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                                                >  1:8131802/1‑140 (MQ=255)

                                                        aCGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaa                                                                               <  1:11167628/140‑1 (MQ=255)

                                                        aCGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGaa                                                                               <  1:243686/140‑1 (MQ=255)

                                                           gTAACGCCTTGCTGGTGCTATGGCAGTGAACCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCATTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAgg                                                                            <  1:3797446/140‑1 (MQ=255)

                                                            tAACGCCTTGCTGGTGCTATGGCAGTGTAACCATCGACATGGATCCAACCCGCCCGAAGGCCATGTGGGGCTTTATCGGCACTGATCGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                           >  2:6646107/1‑140 (MQ=255)

                                                            tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                           >  2:347044/1‑140 (MQ=255)

                                                            tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                           >  2:4902982/1‑140 (MQ=255)

                                                            tAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGc                                                                           >  2:93551/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCGGTGAAACCATCGACTTGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTGAACGGCACTGAACGCCCCGGCGCTGTTTAGCTGGCAGCGGCTCTGGGTGCTCACAGCCAGAAAGGCAt                                                                         >  2:5804945/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCAt                                                                         >  1:10258582/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCAt                                                                         >  2:1940654/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCAt                                                                         >  1:3395384/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCAt                                                                         >  1:1970538/1‑140 (MQ=255)

                                                              aCGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGGTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGcct                                                                         >  2:11260217/1‑138 (MQ=255)

                                                                 ccTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATccc                                                                      >  1:1369203/1‑140 (MQ=255)

                                                                  cTTGCTGGTGCTATGGCAGTGAAACCATCGGCATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCa                                                                     <  2:3083891/140‑1 (MQ=255)

                                                                   ttGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAg                                                                    <  2:3166175/140‑1 (MQ=255)

                                                                     gatggtggTATGGCATTGAAACAATCGACATGGATCCATCCCGCCCGAAGGCCATTTGGGGCTTTATCGGCAGTGAACGCCACGGCGATGTTTACCTGGCAGCGGCTCTGGCAGCTGAATGCCAGTAAGGCATCCCatct                                                                  <  2:11466401/138‑4 (MQ=255)

                                                                     gCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCa                                                                  >  1:9217259/1‑140 (MQ=255)

                                                                      cTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGTACGCCCCGGCGCTGTTTACCTGGCCGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAt                                                                 >  2:1240993/1‑140 (MQ=255)

                                                                         gTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATtct                                                              <  2:11421985/140‑1 (MQ=255)

                                                                         gTGATATGGCAGTGAAACCATCGTCATGGATCCAACCCGCCCGAAGGCCTTTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATtct                                                              <  2:9259851/140‑1 (MQ=255)

                                                                              aTGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAtt                                                         <  1:480881/140‑1 (MQ=255)

                                                                              aTGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCAtt                                                         >  1:4670140/1‑140 (MQ=255)

                                                                                ggCAGTGAAACCATCGACATGGATCCAACCCTCCCGAAGGCCATTTGGGGATTTAACGGCACTGAACGCCCCGGCGATGTTTACCTGGAAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCAATTTa                                                       >  2:4122777/1‑140 (MQ=255)

                                                                                   aGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACgg                                                    <  1:189208/140‑1 (MQ=255)

                                                                                   aGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACgg                                                    >  1:2320556/1‑140 (MQ=255)

                                                                                    gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                   >  1:4495479/1‑140 (MQ=255)

                                                                                    gTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGt                                                   >  1:6513764/1‑140 (MQ=255)

                                                                                     tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTc                                                  >  1:8607880/1‑140 (MQ=255)

                                                                                     tGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTc                                                  >  2:8974358/1‑140 (MQ=255)

                                                                                      gAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGa                                                                                >  2:5724392/1‑109 (MQ=255)

                                                                                      gAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCa                                                 <  2:7517782/140‑1 (MQ=255)

                                                                                        aaCCATCGACATGGATCCAACCCGCCCGAAGGCCCTTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATg                                               <  1:6368337/140‑1 (MQ=255)

                                                                                         aCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGa                                              >  1:836695/1‑140 (MQ=255)

                                                                                             tCGACCTGGATCCAACCCGCCCGCAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCAGGACGtt                                          <  2:9708503/140‑1 (MQ=255)

                                                                                             tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                          <  1:9788299/140‑1 (MQ=255)

                                                                                             tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                          >  1:6804778/1‑140 (MQ=255)

                                                                                             tCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                          >  1:3587050/1‑140 (MQ=255)

                                                                                             tCGACATGGAGCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGtt                                          <  2:10131464/140‑1 (MQ=255)

                                                                                                 cATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                      <  1:5363440/140‑1 (MQ=255)

                                                                                                 cATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAgg                                      <  2:3840817/140‑1 (MQ=255)

                                                                                                       tCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCg                                <  2:7835455/140‑1 (MQ=255)

                                                                                                       tCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCACGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCg                                >  2:6036952/1‑140 (MQ=255)

                                                                                                        ccAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGa                               <  2:691230/140‑1 (MQ=255)

                                                                                                           aCCCGCCCGCAGGCCATGTGGGGCTGTAACGGCCCTGAACGCCCCGGCGCTGTGTACCGGGCAGCGGCTCTGGCAGCTCACAGCCAGCAAGGCATCCCCGCATGCTCCATGTCCGGTCATGACGGTCAGGATGCCGAGGa                            <  2:3641817/140‑1 (MQ=255)

                                                                                                           aCCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGa                            >  2:8457520/1‑140 (MQ=255)

                                                                                                            cccgcccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGac                           <  2:10553722/140‑1 (MQ=255)

                                                                                                            cccgcccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGac                           <  1:5015293/140‑1 (MQ=255)

                                                                                                                cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCCGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCGTTTACGGTCATGACGTTCAGGATGCCGATGAcacag                       >  2:4584766/1‑139 (MQ=255)

                                                                                                                cccgAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACAt                       >  1:9622481/1‑140 (MQ=255)

                                                                                                                cccgAAGGCCATGTGGGGCTTTCACGGCACTGAACGCCCCGGCGCTGTGTACCTGGCAGGGGCTGTGGCAGCTCTCAGCCAGGAGGGCAGCCCGGCATTCGCCAGTTTCGGTCATGACGTGCTGGAGGCCGATGACTCAt                       >  2:2075174/1‑140 (MQ=255)

                                                                                                                    aaGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGAt                   >  1:10475943/1‑140 (MQ=255)

                                                                                                                     cggCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGAtt                  <  1:2053241/139‑1 (MQ=255)

                                                                                                                     aGGCCATTTGGGGCTTTACCGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCAGTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGAtt                  <  2:8750491/140‑1 (MQ=255)

                                                                                                                            ttGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCg           <  2:4287413/140‑1 (MQ=255)

                                                                                                                                   tttAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTga    <  1:5127674/140‑1 (MQ=255)

                                                                                                                                     tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaag  >  1:8664366/1‑140 (MQ=255)

                                                                                                                                     tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaag  >  1:1662123/1‑140 (MQ=255)

                                                                                                                                     tAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTgaag  >  1:566666/1‑140 (MQ=255)

CTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACAGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAG  >  NZ_CP025268/2938085‑2938357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`