BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	308,410	T→C	223 (TAA→TAG)	ecpB ←	fimbrial chaperone EcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	308,410	0	T	C	99.8%	2739.3 / NA	789	223 (TAA→TAG)	ecpB	fimbrial chaperone EcpB
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (285/501); minor base A (1/1); total (287/502)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 806 total aligned reads displayed.

CTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGG  >  NZ_CP025268/308273‑308546

cTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCtatcta                                                                                                                                        <  1:8963409/140‑1 (MQ=255)

         gcgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACggg                                                                                                                               >  1:7372885/1‑140 (MQ=255)

         gcgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACggg                                                                                                                               >  1:4305414/1‑140 (MQ=255)

          cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGa                                                                                                                              <  1:10536897/140‑1 (MQ=255)

          cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGa                                                                                                                              >  1:950173/1‑140 (MQ=255)

          cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGa                                                                                                                              <  1:9857795/140‑1 (MQ=255)

          cgcACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGa                                                                                                                              >  2:6560837/1‑140 (MQ=255)

          cgcACGTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGAACATGCCGCAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCCTCTGCACAGCTATCTCGTTCACGGGa                                                                                                                              >  2:2083766/1‑140 (MQ=255)

             aCTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATg                                                                                                                           <  1:8472105/140‑1 (MQ=255)

             aCTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATg                                                                                                                           <  2:8857344/140‑1 (MQ=255)

             aCTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATg                                                                                                                           <  1:7012530/140‑1 (MQ=255)

             aCTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATg                                                                                                                           >  1:4674468/1‑140 (MQ=255)

                 aTGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACt                                                                                                                       >  1:7682632/1‑140 (MQ=255)

                 aTGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCAGTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACt                                                                                                                       >  1:9144265/1‑140 (MQ=255)

                  tGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCAAGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTAACGATTTCACGGGAATGAACtg                                                                                                                      <  2:9637595/140‑2 (MQ=255)

                    tCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTAt                                                                                                                    <  2:5338903/140‑1 (MQ=255)

                      agcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCa                                                                                                                  >  1:1518136/1‑140 (MQ=255)

                      agcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCa                                                                                                                  >  2:1207030/1‑140 (MQ=255)

                       gcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAc                                                                                                                 >  1:9225807/1‑140 (MQ=255)

                        cagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGTGAGAAACGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATATATTTCACGGGAATGAACTTATCAcc                                                                                                                <  2:1665009/140‑1 (MQ=255)

                        cagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTGTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGGTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAcc                                                                                                                <  2:11245741/140‑1 (MQ=255)

                         agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAccc                                                                                                               <  1:11235114/140‑1 (MQ=255)

                         agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAccc                                                                                                               >  1:749245/1‑140 (MQ=255)

                         agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAccc                                                                                                               >  2:4405763/1‑140 (MQ=255)

                         agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCAccc                                                                                                               >  1:198374/1‑140 (MQ=255)

                           cgcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTg                                                                                                             >  1:3969597/1‑140 (MQ=255)

                             cTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGcc                                                                                                           >  1:5291662/1‑140 (MQ=255)

                              tGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCa                                                                                                          <  1:3738723/140‑1 (MQ=255)

                              tGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCa                                                                                                          <  2:10916957/140‑1 (MQ=255)

                              tGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCa                                                                                                          <  1:11148294/140‑1 (MQ=255)

                              tGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCa                                                                                                          <  1:6835748/140‑1 (MQ=255)

                                   tCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTg                                                                                                     <  1:8668503/140‑1 (MQ=255)

                                   tCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTg                                                                                                     <  1:2214602/140‑1 (MQ=255)

                                     gggCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCa                                                                                                   >  1:3049691/1‑140 (MQ=255)

                                      ggCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCaa                                                                                                  <  2:10385329/140‑1 (MQ=255)

                                       gCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                 <  2:1498277/140‑1 (MQ=255)

                                       gCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                 <  1:10757022/140‑1 (MQ=255)

                                       gCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAAc                                                                                                 <  1:7189429/140‑1 (MQ=255)

                                          gaacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACGTATCACCCTGCCAAAGTGCAACCCg                                                                                              <  2:6967662/140‑1 (MQ=255)

                                           aacgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGt                                                                                             <  2:8047139/140‑1 (MQ=255)

                                            acgaacAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTc                                                                                            <  1:2868782/140‑1 (MQ=255)

                                                acAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt                                                                                        <  2:10021291/140‑1 (MQ=255)

                                                acAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCAGTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCttt                                                                                        <  2:9376297/140‑1 (MQ=255)

                                                acAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCATGGGGAGAACCGTCGTAATGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCCCGGGAATGAACTTATCACCCTGCCAAAGTGCTACCCGTCCttg                                                                                        >  2:774895/1‑139 (MQ=255)

                                                        aCCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGGGAACCGGCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                                <  1:4466387/140‑1 (MQ=255)

                                                        aCCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCg                                                                                <  1:5648065/140‑1 (MQ=255)

                                                          cATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCa                                                                              <  1:1356590/140‑1 (MQ=255)

                                                            tGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAgt                                                                            >  1:10303212/1‑140 (MQ=255)

                                                                 aaGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCa                                                                       >  2:8608176/1‑140 (MQ=255)

                                                                 aaGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGGCGTAACGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCa                                                                       <  2:9182471/140‑1 (MQ=255)

                                                                  aGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                      <  2:4183266/140‑1 (MQ=255)

                                                                  aGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                      <  2:6195403/140‑1 (MQ=255)

                                                                  aGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                      <  2:7559329/140‑1 (MQ=255)

                                                                      aaaCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCgg                                                                  >  2:4646320/1‑140 (MQ=255)

                                                                                 tCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCCACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTTAAacgacg                                                       <  1:6329689/140‑1 (MQ=255)

                                                                                   aGTCCTGGGGAGAACCGTCGTACAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAACGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGAcgcg                                                     <  2:5685714/140‑1 (MQ=255)

                                                                                     tCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGAc                                                   <  1:7812393/140‑1 (MQ=255)

                                                                                          gggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                              <  1:1001228/140‑1 (MQ=255)

                                                                                          gggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                              <  2:8402785/140‑1 (MQ=255)

                                                                                          gggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGcc                                              <  1:6496890/140‑1 (MQ=255)

                                                                                               aaacgtcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACTCGGGTAAAACTACGCGACTTGCCCGGCa                                         >  2:2283552/4‑140 (MQ=255)

                                                                                               aaCCGTCGTCAAGGCATTGTCGTTATCCATCGGCACAGCTATCTCTTTCACGGGACTGAACTTATCACCCTGCCAACGAGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCa                                         <  2:8255408/140‑1 (MQ=255)

                                                                                               aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCa                                         >  1:3457170/1‑140 (MQ=255)

                                                                                               aaCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTGTGTTATCCGCAGTGTCCACGCGGGTACATCGACGCGTCTTGCCCGGCa                                         >  1:9567479/1‑140 (MQ=255)

                                                                                                aCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                        <  1:2398862/140‑1 (MQ=255)

                                                                                                aCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                        <  1:3633226/140‑1 (MQ=255)

                                                                                                 ccGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATc                                       >  1:5491225/1‑140 (MQ=255)

                                                                                                  cgtcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTCTTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGGTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCa                                      <  2:2670894/140‑1 (MQ=255)

                                                                                                    tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg                                    <  1:5845620/140‑1 (MQ=255)

                                                                                                    tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg                                    >  1:119234/1‑140 (MQ=255)

                                                                                                    tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg                                    >  1:5093948/1‑140 (MQ=255)

                                                                                                    tcgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAgg                                    <  1:7932722/140‑1 (MQ=255)

                                                                                                     cgtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgt                                   <  1:11345793/140‑1 (MQ=255)

                                                                                                       tAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtag                                 <  1:8290718/140‑1 (MQ=255)

                                                                                                       tAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtag                                 <  2:1064748/140‑1 (MQ=255)

                                                                                                         aaGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtagta                               >  2:5557906/1‑140 (MQ=255)

                                                                                                           ggCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAt                             <  1:11028464/140‑1 (MQ=255)

                                                                                                           ggCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAt                             <  1:4876025/140‑1 (MQ=255)

                                                                                                             cATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGAGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtc                           <  2:6873374/140‑1 (MQ=255)

                                                                                                              cttGTCGTTATCCATCTGCACAGCTATCTATTTCCCGGGAATGAACTGATCACCCTGCCAAAGTGCAACCCGTCCTTGGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct                          <  2:452506/139‑1 (MQ=255)

                                                                                                              aTTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct                          >  1:6099992/1‑140 (MQ=255)

                                                                                                              aTTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATtct                          <  2:7220605/140‑1 (MQ=255)

                                                                                                                   cGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTAGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTa                     <  2:2402077/140‑1 (MQ=255)

                                                                                                                   cGTCATCCATCTGCACAGCTATCTATAACACGGCAATGAACGTATCACCCTGCCAACGTGCCACCCGTCCTTTGTAAACCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGAAGTAATTCTCTTTa                     <  2:9835724/140‑1 (MQ=255)

                                                                                                                    gTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTac                    <  2:5091684/140‑1 (MQ=255)

                                                                                                                    gTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACATATCACCCTGCCAAAGTGCAACCCGTCCTATGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATGCTCTTTac                    <  2:3852558/140‑1 (MQ=255)

                                                                                                                     ttATCCATCTGCACAGCTATCTATTTCACGTGAATGATCTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca                   <  2:7386303/140‑1 (MQ=255)

                                                                                                                     ttATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca                   <  1:1737507/140‑1 (MQ=255)

                                                                                                                     ttATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTaca                   <  1:8368233/140‑1 (MQ=255)

                                                                                                                      tATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTacac                  >  2:11374619/1‑140 (MQ=255)

                                                                                                                       aTCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACt                 >  1:2654050/1‑140 (MQ=255)

                                                                                                                            tCTGCACAGCTCTCTATTTCACGGGAATGAACTTCTCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCCTCAGGTAGTAATTCTCTGTACACTCCtga            >  2:8514505/1‑138 (MQ=255)

                                                                                                                            tCTGCACAGCTATCTCTTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAGTCTCTTTACACTCCTTa            >  2:11575145/1‑140 (MQ=255)

                                                                                                                            tCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCAGCCAATGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTa            <  2:3089971/140‑1 (MQ=255)

                                                                                                                            gatcaacaTCTATCTATTTAACCGGAATTAAAAAAACACACTGCCAAAGTGCAACCCGAAATTTTTTATCCGAAGTGTACACGCGTGTAAAACGACGCGACTTGCACTGCATCTTTAAGTAATTCTCTTTACACTCATTa            >  2:584077/6‑140 (MQ=255)

                                                                                                                             cTGCACAGCTATCTATTTCACGGGAATGACCTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTCTCCGCAGTGTCCACGCGGGGAAAACGACGCGACTTGCCCGGCATCAGGGAGTAATTCTCTTTACACTGCTTAc           >  2:3360149/1‑140 (MQ=255)

                                                                                                                                   aGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTgg     <  1:3350861/140‑1 (MQ=255)

                                                                                                                                     ctatctatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg   >  2:1866987/1‑140 (MQ=255)

                                                                                                                                     ctatctatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg   <  1:663513/140‑1 (MQ=255)

                                                                                                                                     ctatctatTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg   >  1:6898287/1‑140 (MQ=255)

CTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATTTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGG  >  NZ_CP025268/308273‑308546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`