BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,940,107	G→A	G112D (GGC→GAC)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,940,107	0	G	A	99.6%	2746.4 / ‑4.7	727	G112D (GGC→GAC)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (306/415); total (309/418)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 727 total aligned reads displayed.

TAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939970‑2940243

tAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACa                                                                                                                                        >  2:5240370/1‑140 (MQ=255)

tAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACa                                                                                                                                        >  2:3948196/1‑140 (MQ=255)

tAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACa                                                                                                                                        <  1:1757550/140‑1 (MQ=255)

     aaCTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTCTGGACAATAtt                                                                                                                                   >  2:8432604/1‑140 (MQ=255)

      actgactgAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTg                                                                                                                                  <  1:197665/140‑1 (MQ=255)

        tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCTCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCAGCGAACAGCAGCGTTTATGGACAATATTGaa                                                                                                                                <  2:1069778/140‑1 (MQ=255)

        tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  2:3486594/140‑1 (MQ=255)

        tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  1:9265352/140‑1 (MQ=255)

        tgactgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGaa                                                                                                                                <  1:5492128/140‑1 (MQ=255)

           ctgaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACgg                                                                                                                             <  2:7850448/140‑1 (MQ=255)

             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:10528601/140‑1 (MQ=255)

             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           >  1:4520428/1‑140 (MQ=255)

             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           >  1:8601579/1‑140 (MQ=255)

             gaATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGtt                                                                                                                           <  2:5783663/140‑1 (MQ=255)

                tGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAt                                                                                                                        >  1:5725142/1‑140 (MQ=255)

                tGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAt                                                                                                                        >  1:6717235/1‑140 (MQ=255)

                tGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAt                                                                                                                        <  2:2915595/140‑1 (MQ=255)

                      aTCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGc                                                                                                                  >  2:7563669/1‑140 (MQ=255)

                        ccGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGcac                                                                                                                <  2:3081329/140‑1 (MQ=255)

                                tCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTg                                                                                                        >  1:10793781/1‑140 (MQ=255)

                                    cGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGTTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAgg                                                                                                    <  1:7417469/140‑1 (MQ=255)

                                    cGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAAAGGTTAATCTCCGCACAGCGGTTGCAgg                                                                                                    <  1:7913267/140‑1 (MQ=255)

                                      tCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCt                                                                                                  >  1:518093/1‑140 (MQ=255)

                                        accaccTTTGGTGTGGATGGCGCTCTGGTAGGTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTAt                                                                                                >  1:6624539/1‑140 (MQ=255)

                                        accaccTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTAt                                                                                                >  1:7142460/1‑140 (MQ=255)

                                        accaccTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTAt                                                                                                >  1:9880220/1‑140 (MQ=255)

                                                tGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTCTCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCCGGCTATTTCTGGCg                                                                                        >  2:2717538/1‑140 (MQ=255)

                                                  gtgtGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTc                                                                                      <  2:2292499/140‑1 (MQ=255)

                                                    gtgGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATACGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCgg                                                                                    <  2:7225101/140‑1 (MQ=255)

                                                       gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                                 >  1:4151068/1‑140 (MQ=255)

                                                       gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                                 >  2:10020677/1‑140 (MQ=255)

                                                       gATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGc                                                                                 >  1:8026707/1‑140 (MQ=255)

                                                         tcgcgcTCTGGTAGATAAGCAAGGCATTCTGCTCTATCAGATTATTAGCTGGACATGTCCGCGAAAAGCAGCTGTTATGTACAATATTGAACGGTTAATCTACGCACAGAGGTAGCAGGATATTTCTGTCTTGGGAGCCt                                                                               >  2:10244996/3‑140 (MQ=255)

                                                          ggCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCtt                                                                              <  1:4667496/140‑1 (MQ=255)

                                                           gcgcTCTGGTAGATAAGCAAGGCAATCTTCTCTATCCGAGTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAAGCTCCGCACAGCGGTTGCAGGCTATTTCTGTCGTCGGAGCCttt                                                                             <  2:5143937/140‑1 (MQ=255)

                                                            cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTCTCCGAGTATTCGCTGGAAATGTCCGCGAACAGCAGCGGGTTTGGACACGATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTGTGGCGTCGGAGGCtgtg                                                                            >  2:8090742/1‑137 (MQ=255)

                                                            cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTa                                                                            >  2:5560956/1‑140 (MQ=255)

                                                            cgcTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTa                                                                            >  1:4369728/1‑140 (MQ=255)

                                                                 tGGTAGATAAGCAAGGCAATCTGCTCTCTCCGATTATTAGCTGGAAATGTCAGCGAACAGCAGCGGTTATGGTCAATATTGAACGGGTCATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTc                                                                       >  2:10792736/1‑140 (MQ=255)

                                                                 tGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTc                                                                       >  1:5014342/1‑140 (MQ=255)

                                                                 tGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTc                                                                       >  2:4642424/1‑140 (MQ=255)

                                                                  ggTAGATAAGCAAGGCAATCTGCTCTCTCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGGTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  2:3832386/140‑1 (MQ=255)

                                                                  ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  2:9372511/140‑1 (MQ=255)

                                                                  ggTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCa                                                                      <  1:10573193/140‑1 (MQ=255)

                                                                   gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                     <  1:11569152/140‑1 (MQ=255)

                                                                   gTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCaa                                                                     >  1:11233777/1‑140 (MQ=255)

                                                                     aGATAAGCAGGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:6692357/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCTTTTAGTTTCAATa                                                                   <  1:10017554/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:3803133/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  2:3224521/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:2812377/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:2362573/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:219581/140‑1 (MQ=255)

                                                                     aGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATa                                                                   <  1:1424386/140‑1 (MQ=255)

                                                                       aTAAGCAATGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:4918128/140‑1 (MQ=255)

                                                                       aTAAGCAAGGCATTCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:10978838/140‑1 (MQ=255)

                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:656505/140‑1 (MQ=255)

                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:3271015/140‑1 (MQ=255)

                                                                       aTAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  1:3996707/140‑1 (MQ=255)

                                                                       aTAAGCAACGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACg                                                                 <  2:1860412/140‑1 (MQ=255)

                                                                         aaGCCAGGCACTCTGCTCTAGCCGATTAGTAGCTGGAAATGTCCGCGAACCGCAGCGGTTATGGACACTATTGAACGGTTAAGCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGtt                                                               <  2:9142713/140‑1 (MQ=255)

                                                                         aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGtt                                                               >  1:6002490/1‑140 (MQ=255)

                                                                         aaGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGtt                                                               >  2:11122774/1‑140 (MQ=255)

                                                                           gcaatgcaatCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             <  1:2955237/140‑1 (MQ=255)

                                                                           gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             <  1:9145322/140‑1 (MQ=255)

                                                                           gCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtat                                                             >  1:3479954/1‑140 (MQ=255)

                                                                            cAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTtata                                                            <  2:4383012/140‑1 (MQ=255)

                                                                                   aTCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTg                                                     <  1:8983667/140‑1 (MQ=255)

                                                                                    tCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGGTCTATAAGTTgg                                                    >  2:2556888/1‑140 (MQ=255)

                                                                                      tGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtg                                                  <  1:10389549/140‑1 (MQ=255)

                                                                                      tGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGgtg                                                  <  1:10501570/140‑1 (MQ=255)

                                                                                            aTCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACTGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTg                                            >  2:3568740/1‑140 (MQ=255)

                                                                                             tCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCGGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTga                                           >  1:6908962/1‑140 (MQ=255)

                                                                                              ccGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaa                                          <  2:3332449/140‑1 (MQ=255)

                                                                                                 attattAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaaga                                       <  2:11474417/140‑1 (MQ=255)

                                                                                                  ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  1:6596970/1‑140 (MQ=255)

                                                                                                  ttattaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  2:1451029/1‑140 (MQ=255)

                                                                                                  ttattaGCTGGAAATGTCCGCGAACAGAAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTgaaagaa                                      >  2:6271554/1‑140 (MQ=255)

                                                                                                    attaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGaaaa                                    <  1:6190270/140‑1 (MQ=255)

                                                                                                    attaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTCTATAAGTGGGGGTGGTTGAAAGaaaa                                    <  2:1766142/140‑1 (MQ=255)

                                                                                                    attaGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGaaaa                                    <  1:9632069/140‑1 (MQ=255)

                                                                                                       aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGGACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                 >  1:1558761/1‑140 (MQ=255)

                                                                                                       aGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAatca                                 >  1:2595703/1‑140 (MQ=255)

                                                                                                            gccaTGGCCGCGCACATCAGCGGAGATGGACAAGATGGCACGGTTAATCGCCGCACAGCGGTTGCAGGCGATTTCTGGAGTCGGAGCCTTTCGTTTCAATACGTTACTTACGTTGGTGAGGGTGAAAGAACATCATCcac                            <  2:1619519/137‑1 (MQ=255)

                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGTACAATATTGAACGGATAATCTCCGCACAGCGGTTGCAGGCTATTTCTGTAGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            <  2:6890385/140‑1 (MQ=255)

                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCcac                            >  1:3062498/1‑140 (MQ=255)

                                                                                                            gAAATGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCaac                            >  2:9710030/1‑140 (MQ=255)

                                                                                                              aaTGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACaa                          <  2:8760136/140‑1 (MQ=255)

                                                                                                              aaTGTCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTATAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACaa                          <  1:8356465/140‑1 (MQ=255)

                                                                                                                  tCCGCGAACAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAActgc                      >  2:2117197/1‑140 (MQ=255)

                                                                                                                         aCAGCAGCGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTNTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcg               <  1:6307309/140‑1 (MQ=255)

                                                                                                                            gcagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCATTACGTTATATAAGTTGGTGTGGGTGAAAGAAAATCATCCACAACTGCTGGAAcgcgc            >  1:9435389/1‑140 (MQ=255)

                                                                                                                             cagcGGTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAAcgcgcg           <  1:8203939/140‑1 (MQ=255)

                                                                                                                              agcGGTTATCGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGCCAATTTCTGGCGTCGGAGCCTTTAGTTACAATACGTTATATAAGATGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACgcgcgc          <  2:1188778/140‑1 (MQ=255)

                                                                                                                                  gTTATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCGTTTAGTTTCATTACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGc      >  1:4912112/1‑140 (MQ=255)

                                                                                                                                   ttATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGcc     >  1:7668143/1‑140 (MQ=255)

                                                                                                                                   ttATGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGcc     <  2:602766/140‑1 (MQ=255)

                                                                                                                                     aTGGACAATATTGATCGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTg   <  2:4236302/140‑1 (MQ=255)

                                                                                                                                      tGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTgg  <  2:10433809/140‑1 (MQ=255)

                                                                                                                                      tGGACAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTgg  <  2:96241/140‑1 (MQ=255)

TAGTGAACTGACTGAATGCCACATCCGCGGTATCGCCGTCACCACCTTTGGTGTGGATGGCGCTCTGGTAGATAAGCAAGGCAATCTGCTCTATCCGATTATTAGCTGGAAATGTCCGCGAACAGCAGCGGTTATGGGCAATATTGAACGGTTAATCTCCGCACAGCGGTTGCAGGCTATTTCTGGCGTCGGAGCCTTTAGTTTCAATACGTTATATAAGTTGGTGTGGTTGAAAGAAAATCATCCACAACTGCTGGAACGCGCGCACGCCTGG  >  NZ_CP025268/2939970‑2940243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`